The main purpose of this study was to investigate the protective actions of captopril and cicaprost on changes of membrane fluidity of cultured neonatal rat myocardial cells exposed to anoxia and sugar deprivation.Lip...The main purpose of this study was to investigate the protective actions of captopril and cicaprost on changes of membrane fluidity of cultured neonatal rat myocardial cells exposed to anoxia and sugar deprivation.Lipid peroxidation level estimated by determining the thiobarbituric acid reactive substance(TBARS)content and lactate dehydrogenase(LDH)released in culture medium was also observed in order to examine other membrane-related changes due to anoxia.Membrane fluidity was monitored by measuring changes in the steady state fluorescence anisotropy(r_s)by fluorescence spectroscopy.The r_s value,TBARS level and LDH release were significantly increased after 3 h anoxia.Captopril(180 μmol/L),cicaprost(30 nmol/L)and indomethacin(1μmol/L)did not alter r_s, TBARS level and LDH activity of normal cultured neonatal rat myocardial cells.However,both captopril and cicaprost significantly prevented the increases of r_s,TBARS content and LDH release in those cells exposed to anoxia and sugar deprivation.lndomethacin abolished the actions of captopril on TBARS production and LDH release,but maintained its membrane fluidity protection.These results indicate that captopril and cicaprost protect membrane fluidity and lipid peroxidation changes in anoxia- injured myocardial cells.The action mechanism of captopril may be due,in part,to stimulation of prostacyclin synthesis and/or release.展开更多
An ensemble adjustment Kalman filter system is developed to assimilate Argo profiles into the Northwest Pacific MASNUM wave-circulation coupled model, which is based on the Princeton Ocean Model (POM). This model was ...An ensemble adjustment Kalman filter system is developed to assimilate Argo profiles into the Northwest Pacific MASNUM wave-circulation coupled model, which is based on the Princeton Ocean Model (POM). This model was recoded in FORTRAN-90 style, and some new data types were defined to improve the efficiency of system design and execution. This system is arranged for parallel computing by using UNIX shell scripts: it is easier with single models running separately with the required information exchanged through input/output files. Tests are carried out to check the performance of the system: one for checking the ensemble spread and another for the performance of assimilation of the Argo data in 2005. The first experiment shows that the assimilation system performs well. The comparison with the Satellite derived sea surface temperature (SST) shows that modeled SST errors are reduced after assimilation; at the same time, the spatial correlation between the simulated SST anomalies and the satellite data is improved because of Argo assimilation. Furthermore, the temporal evolution/trend of SST becomes much better than those results without data assimilation. The comparison against GTSPP profiles shows that the improvement is not only in the upper layers of ocean, but also in the deeper layers. All these results suggest that this system is potentially capable of reconstructing oceanic data sets that are of high quality and are temporally and spatially continuous.展开更多
基金Supported by a grant for young researcher from Ministry of Public Health of P.R.C.
文摘The main purpose of this study was to investigate the protective actions of captopril and cicaprost on changes of membrane fluidity of cultured neonatal rat myocardial cells exposed to anoxia and sugar deprivation.Lipid peroxidation level estimated by determining the thiobarbituric acid reactive substance(TBARS)content and lactate dehydrogenase(LDH)released in culture medium was also observed in order to examine other membrane-related changes due to anoxia.Membrane fluidity was monitored by measuring changes in the steady state fluorescence anisotropy(r_s)by fluorescence spectroscopy.The r_s value,TBARS level and LDH release were significantly increased after 3 h anoxia.Captopril(180 μmol/L),cicaprost(30 nmol/L)and indomethacin(1μmol/L)did not alter r_s, TBARS level and LDH activity of normal cultured neonatal rat myocardial cells.However,both captopril and cicaprost significantly prevented the increases of r_s,TBARS content and LDH release in those cells exposed to anoxia and sugar deprivation.lndomethacin abolished the actions of captopril on TBARS production and LDH release,but maintained its membrane fluidity protection.These results indicate that captopril and cicaprost protect membrane fluidity and lipid peroxidation changes in anoxia- injured myocardial cells.The action mechanism of captopril may be due,in part,to stimulation of prostacyclin synthesis and/or release.
基金Supported by the Project of National Basic Research Program of China (No. 2007CB816002)Special Fund for Fundamental Scientific Research (No. 2008G08)
文摘An ensemble adjustment Kalman filter system is developed to assimilate Argo profiles into the Northwest Pacific MASNUM wave-circulation coupled model, which is based on the Princeton Ocean Model (POM). This model was recoded in FORTRAN-90 style, and some new data types were defined to improve the efficiency of system design and execution. This system is arranged for parallel computing by using UNIX shell scripts: it is easier with single models running separately with the required information exchanged through input/output files. Tests are carried out to check the performance of the system: one for checking the ensemble spread and another for the performance of assimilation of the Argo data in 2005. The first experiment shows that the assimilation system performs well. The comparison with the Satellite derived sea surface temperature (SST) shows that modeled SST errors are reduced after assimilation; at the same time, the spatial correlation between the simulated SST anomalies and the satellite data is improved because of Argo assimilation. Furthermore, the temporal evolution/trend of SST becomes much better than those results without data assimilation. The comparison against GTSPP profiles shows that the improvement is not only in the upper layers of ocean, but also in the deeper layers. All these results suggest that this system is potentially capable of reconstructing oceanic data sets that are of high quality and are temporally and spatially continuous.
