Objective To study the cell growth factor secretion and vascular regeneration in acute in-farcted myocardium after autologous skeletal muscle satellite cell implantation. Methods Autologous skeletal muscle satellite c...Objective To study the cell growth factor secretion and vascular regeneration in acute in-farcted myocardium after autologous skeletal muscle satellite cell implantation. Methods Autologous skeletal muscle satellite cells from adult mongrel canine were implanted into the acute myocardial infarct site via the ligated left anterior descending (LAD) artery. Specimens were harvested at 2, 4 , 8 weeks after implantation for the expression of insulin-like growth factor-1 (IGF-1), basic fibroblast growth factor ( bFGF) and the vascular density. Results The expression of IGF-1, bFGF and the vascular density in skeletal muscle satellite cell implant group were higher than that in the control group. Conclusion The skeletal muscle satellite cells, after being implanted into the acute myocardial infarction, not only showed myocardial regeneration, but also showed the ability to secrete the cell factors, hence representing a positive effect on the regeneration of the infarcted myocardium.展开更多
To study the effect of different access routes on autologous satellite cell implantation to stimulate myocardial regeneration Methods Satellite cells were procured from skeletal muscle (gluteus max) of adult mongrel...To study the effect of different access routes on autologous satellite cell implantation to stimulate myocardial regeneration Methods Satellite cells were procured from skeletal muscle (gluteus max) of adult mongrel canine, cultured, proliferated and labeled with 4’,6 diamidino 2 phenylindone (DAPI) in vitro The cells were autologously implanted into the site of acute myocardial infarction by local injection or perfusion through the ligated distal left anterior descending coronary artery Specimens were harvested 2, 4 and 8 weeks later for histological study Results The labeling efficiency of satellite cells with DAPI was close to 100% Fluorescent cells were found at the infarcted zone, papillary muscle and local injection site Some of these cells had progressively differentiated into striated muscle fibers connected to intercalated discs The infant cells appeared different from the mature myocardium under an electron microscope Satellite cells implanted by perfusion through the coronary artery were arranged in order of consistency with host myocardial fibers The satellite cells, implanted by local injection, were found growing in a disordered way Conclusion Satellite cells, implanted by coronary artery perfusion, can progressively differentiate into striated muscle fibers, arranging in order and disseminating over the infarcted zone This approach seems more favorable for the recovery of myocardial contractile function than that of local injection展开更多
基金Supported by grants from the Nature Science Foundation of China(39770735)
文摘Objective To study the cell growth factor secretion and vascular regeneration in acute in-farcted myocardium after autologous skeletal muscle satellite cell implantation. Methods Autologous skeletal muscle satellite cells from adult mongrel canine were implanted into the acute myocardial infarct site via the ligated left anterior descending (LAD) artery. Specimens were harvested at 2, 4 , 8 weeks after implantation for the expression of insulin-like growth factor-1 (IGF-1), basic fibroblast growth factor ( bFGF) and the vascular density. Results The expression of IGF-1, bFGF and the vascular density in skeletal muscle satellite cell implant group were higher than that in the control group. Conclusion The skeletal muscle satellite cells, after being implanted into the acute myocardial infarction, not only showed myocardial regeneration, but also showed the ability to secrete the cell factors, hence representing a positive effect on the regeneration of the infarcted myocardium.
基金ThisstudywassupportedbyagrantfromtheNationalNaturalScienceFoundationofChina (No 39770 735 )
文摘To study the effect of different access routes on autologous satellite cell implantation to stimulate myocardial regeneration Methods Satellite cells were procured from skeletal muscle (gluteus max) of adult mongrel canine, cultured, proliferated and labeled with 4’,6 diamidino 2 phenylindone (DAPI) in vitro The cells were autologously implanted into the site of acute myocardial infarction by local injection or perfusion through the ligated distal left anterior descending coronary artery Specimens were harvested 2, 4 and 8 weeks later for histological study Results The labeling efficiency of satellite cells with DAPI was close to 100% Fluorescent cells were found at the infarcted zone, papillary muscle and local injection site Some of these cells had progressively differentiated into striated muscle fibers connected to intercalated discs The infant cells appeared different from the mature myocardium under an electron microscope Satellite cells implanted by perfusion through the coronary artery were arranged in order of consistency with host myocardial fibers The satellite cells, implanted by local injection, were found growing in a disordered way Conclusion Satellite cells, implanted by coronary artery perfusion, can progressively differentiate into striated muscle fibers, arranging in order and disseminating over the infarcted zone This approach seems more favorable for the recovery of myocardial contractile function than that of local injection
