Shewanella marisflavi strain AP629 was certified as a novel pathogen of the sea cucumber Apostichopusjaponicus. In this study, four monoclonal antibodies （MAbs） （3C1, 3D9, 2F2, 2A8） against strain AP629 were devel...Shewanella marisflavi strain AP629 was certified as a novel pathogen of the sea cucumber Apostichopusjaponicus. In this study, four monoclonal antibodies （MAbs） （3C1, 3D9, 2F2, 2A8） against strain AP629 were developed by immunizing Balb/C mice. 3C1 and 3D9 recognized S. marisflavi only, showing no cross reactivity to other gram-negative bacteria, However, 2F2 and 2A8 showed cross reactivity to all tested bacteria. Indirect immunofluorescence, and immunogold electron microscopy, showed the binding antigens of 3C1 and 3D9 were located at the secretion on the surface of strain AP629. The binding antigens of 2F2 and 2A8 were noted on the membrane of the cells. MAbs 3C1 and 3D9 recognized the lipopolysaccharide fraction of strain AP629, and 2F2 and 2A8 recognized in western-blotting protein antigens with molecular weights of 113 and 128 kDa respectively. MAbs 3C1 and 3D9 have the potential for use in pathogen diagnosis, epidemiology and studies on the mechanism of how S. marisflavi infects A. japonicus. Imrnunohistochemistry with 3C1 or 3D9 identified strain AP629 in the body wall of infected A. japonicus. In the adult sea cucumbers that were infected via body wall injection, positive signals were observed at the site of skin ulceration, and at the connective tissue of the non-ulcerated body wall. In addition, some large blue-stained cells aggregated at the connective tissue colonized by large numbers of bacteria. In juveniles infected via immersion infection, positive signals were observed at the cuticle of the body wall only. Our results suggest that 3C1 and 3D9 could be used in various immunological assays to study the invasion mechanism of strain AP629 in A. japonicus, the law of bacterial colonization, proliferation in different tissues of A. japonicus, and correlation between secretion on the surface of strain AP629 and its pathogenesis to A. japonicus.展开更多
Direct writing of graphene patterns and devices may significantly facilitate the application of graphene-based flexible electronics. In terms of scalability and cost efficiency, inkjet printing is very competitive ove...Direct writing of graphene patterns and devices may significantly facilitate the application of graphene-based flexible electronics. In terms of scalability and cost efficiency, inkjet printing is very competitive over other existing direct- writing methods. However, it has been challenging to obtain highly stable and clog-free graphene-based ink. Here, we report an alternative and highly efficient technique to directly print a reducing reagent on graphene oxide film to form conductive graphene patterns. By this ＂inkjet reduction＂ method, without using any other microfabrication technique, conductive graphene patterns and devices for various applications are obtained. The ionic nature of the reductant ink makes it clog-free and stable for continuous and large-area printing. The method shows self-limited reduction feature, which enables electrical conductivity of graphene patterns to be tuned within 5 orders of magnitude, reaching as high as 8,000 S.m-1. Furthermore, this method can be extended to produce noble metal/graphene composite patterns. The devices, including transistors, biosensors, and surface- enhanced Raman scattering substrates, demonstrate excellent functionalities. This work provides a new strategy to prepare large-area graphene-based devices that is low-cost and highly efficient, promising to advance research on graphene- based flexible electronics.展开更多
Surface texture patterns have great potential for improving tribological performance in terms of reducing friction and wear. The most common methods for surface texatring are laser and injection molding. The 3D printi...Surface texture patterns have great potential for improving tribological performance in terms of reducing friction and wear. The most common methods for surface texatring are laser and injection molding. The 3D printing method is also used to build parts, patterns, and molds that feature fine details for a wide range of applications because texture manufacturing by 3D printing is faster, more flexible, and less expensive than traditional techniques. To date, there has been no research on textured surfaces produced by 3D printing. Therefore, a new fabrication method using 3D printing to improve friction and wear properties is a topic worth exploring. In this study, a reciprocating friction tester was used to evaluate the friction and wear properties of different surface textures produced by 3D printing. The surface of specimens was examined by electron microscope and scanning electron microscope before and after the test. The results show that surface texturing can be applied to 3D printed parts to improve their friction and wear performance.展开更多
To observe the therapeutic efficacy of music electric stimulation of points in treating anxiety. Methods： By adopting a design of multi-centered randomized controlled trial （RCT）, a total of 270 patients with anxie...To observe the therapeutic efficacy of music electric stimulation of points in treating anxiety. Methods： By adopting a design of multi-centered randomized controlled trial （RCT）, a total of 270 patients with anxiety were randomized into a treatment group and a medication group. The treatment group was intervened by music electric stimulation of points, while the medication group was intervened by oral administration of doxepin. The two groups were evaluated by using Hamilton anxiety rating scale （HAMA） and Chinese revised edition of self-rating anxiety scale （SAS-CR） before and after the intervention. The therapeutic efficacies were also compared. Results： The total effective rate was 93.6% in the treatment group versus 92.3% in the medication group, and the between-group difference was statistically insignificant （P〉O.05）. After the treatment, the aggregate scores of HAMA and SAS-CR were significantly changed in both groups （both P〈0.001）, and the inter-group differences were statistically insignificant （P〉O.05）. Conclusion： Music electric stimulation of points can produce equivalent efficacy in treating anxiety compared to doxepin. Thus, it can be taken as a choice in the treatment of anxiety.展开更多
基金Supported by the National Natural Science Foundation of China (Nos. 30800853 and 30901107)the National Key Projects, National Science and Technology Pillar Program during the 12th Five-Year-Plan (No. 2011BAD13B03)
文摘Shewanella marisflavi strain AP629 was certified as a novel pathogen of the sea cucumber Apostichopusjaponicus. In this study, four monoclonal antibodies （MAbs） （3C1, 3D9, 2F2, 2A8） against strain AP629 were developed by immunizing Balb/C mice. 3C1 and 3D9 recognized S. marisflavi only, showing no cross reactivity to other gram-negative bacteria, However, 2F2 and 2A8 showed cross reactivity to all tested bacteria. Indirect immunofluorescence, and immunogold electron microscopy, showed the binding antigens of 3C1 and 3D9 were located at the secretion on the surface of strain AP629. The binding antigens of 2F2 and 2A8 were noted on the membrane of the cells. MAbs 3C1 and 3D9 recognized the lipopolysaccharide fraction of strain AP629, and 2F2 and 2A8 recognized in western-blotting protein antigens with molecular weights of 113 and 128 kDa respectively. MAbs 3C1 and 3D9 have the potential for use in pathogen diagnosis, epidemiology and studies on the mechanism of how S. marisflavi infects A. japonicus. Imrnunohistochemistry with 3C1 or 3D9 identified strain AP629 in the body wall of infected A. japonicus. In the adult sea cucumbers that were infected via body wall injection, positive signals were observed at the site of skin ulceration, and at the connective tissue of the non-ulcerated body wall. In addition, some large blue-stained cells aggregated at the connective tissue colonized by large numbers of bacteria. In juveniles infected via immersion infection, positive signals were observed at the cuticle of the body wall only. Our results suggest that 3C1 and 3D9 could be used in various immunological assays to study the invasion mechanism of strain AP629 in A. japonicus, the law of bacterial colonization, proliferation in different tissues of A. japonicus, and correlation between secretion on the surface of strain AP629 and its pathogenesis to A. japonicus.
文摘Direct writing of graphene patterns and devices may significantly facilitate the application of graphene-based flexible electronics. In terms of scalability and cost efficiency, inkjet printing is very competitive over other existing direct- writing methods. However, it has been challenging to obtain highly stable and clog-free graphene-based ink. Here, we report an alternative and highly efficient technique to directly print a reducing reagent on graphene oxide film to form conductive graphene patterns. By this ＂inkjet reduction＂ method, without using any other microfabrication technique, conductive graphene patterns and devices for various applications are obtained. The ionic nature of the reductant ink makes it clog-free and stable for continuous and large-area printing. The method shows self-limited reduction feature, which enables electrical conductivity of graphene patterns to be tuned within 5 orders of magnitude, reaching as high as 8,000 S.m-1. Furthermore, this method can be extended to produce noble metal/graphene composite patterns. The devices, including transistors, biosensors, and surface- enhanced Raman scattering substrates, demonstrate excellent functionalities. This work provides a new strategy to prepare large-area graphene-based devices that is low-cost and highly efficient, promising to advance research on graphene- based flexible electronics.
基金supported by the Basic Science Research Program of the National Research Foundation of Korea(NRF)funded by the Ministry of Education,Science and Technology of the Korean government(Grant No.NRF-2015R1D1A1A09060901)Ministry of Trade,Industry and Energy via FY 2015 Korea Institute for the Advancement of technology through Construction Machine R&D Expert Cultivation Program
文摘Surface texture patterns have great potential for improving tribological performance in terms of reducing friction and wear. The most common methods for surface texatring are laser and injection molding. The 3D printing method is also used to build parts, patterns, and molds that feature fine details for a wide range of applications because texture manufacturing by 3D printing is faster, more flexible, and less expensive than traditional techniques. To date, there has been no research on textured surfaces produced by 3D printing. Therefore, a new fabrication method using 3D printing to improve friction and wear properties is a topic worth exploring. In this study, a reciprocating friction tester was used to evaluate the friction and wear properties of different surface textures produced by 3D printing. The surface of specimens was examined by electron microscope and scanning electron microscope before and after the test. The results show that surface texturing can be applied to 3D printed parts to improve their friction and wear performance.
基金supported by Scientific Project of the Third Hospital of Mianyang,Sichuan Province,No.(12) 2013~~
文摘To observe the therapeutic efficacy of music electric stimulation of points in treating anxiety. Methods： By adopting a design of multi-centered randomized controlled trial （RCT）, a total of 270 patients with anxiety were randomized into a treatment group and a medication group. The treatment group was intervened by music electric stimulation of points, while the medication group was intervened by oral administration of doxepin. The two groups were evaluated by using Hamilton anxiety rating scale （HAMA） and Chinese revised edition of self-rating anxiety scale （SAS-CR） before and after the intervention. The therapeutic efficacies were also compared. Results： The total effective rate was 93.6% in the treatment group versus 92.3% in the medication group, and the between-group difference was statistically insignificant （P〉O.05）. After the treatment, the aggregate scores of HAMA and SAS-CR were significantly changed in both groups （both P〈0.001）, and the inter-group differences were statistically insignificant （P〉O.05）. Conclusion： Music electric stimulation of points can produce equivalent efficacy in treating anxiety compared to doxepin. Thus, it can be taken as a choice in the treatment of anxiety.
