OBJECTIVE To identify metastasis-related biomarkers in humanovarian cancer cell lines and in serum.METHODS We isolated total protein from cell lysis solutionsand cultured supernatants from 2 human ovarian cancer cell ...OBJECTIVE To identify metastasis-related biomarkers in humanovarian cancer cell lines and in serum.METHODS We isolated total protein from cell lysis solutionsand cultured supernatants from 2 human ovarian cancer cell linesand used SELDI-TOF-MS to detect the differential expressionof the proteins in the 2 cell lines.The proteomic spectra weregenerated using weak cation exchange chips.The biomarkerswere validated by analyzing serum proteins or peptides in ovariancancer patients,relapsed ovarian cancer patients,patients withbenign ovarian tumors,and healthy people.RESULTS Four proteins in the culture supernatant fromHO-8910PM cells were up-regulated,relative to the culturesupernatant of HO-8910 cells.One protein (3,144 Da m/z value)was up-regulated in both the cell lysis solution and in the culturesupernatant of HO-8910PM cells.In addition,expression of the3,144 Da m/z protein differed significantly between serum fromthe 26 ovarian cancer patients,from the 22 relapsed ovarianpatients and from the 37 healthy women (P<0.01).However,therewas no difference between patients with benign ovarian tumorsand healthy people (P>0.5).CONCLUSION Ovarian cancer cell lines with high or lowmetastatic potential have distinct protein profiles.Protein 3,144Da m/z could be a useful biomarker for diagnosing ovarian cancermetastasis.展开更多
Epithelial ovarian cancer(EOC) is the leading cause of death among all gynecological malignancies. Despite the technological and medical advances over the past four decades, such as the development of several biologic...Epithelial ovarian cancer(EOC) is the leading cause of death among all gynecological malignancies. Despite the technological and medical advances over the past four decades, such as the development of several biological markers(mRNA and proteins biomarkers), the mortality rate of ovarian cancer remains a challenge because of its late diagnosis, which is specifically attributed to low specificities and sensitivities. Under this compulsive scenario, recent advances in expression biology have shifted in identifying and developing specific and sensitive biomarkers, such as micro RNAs(miRNAs) for cancer diagnosis and prognosis. MiRNAs are a novel class of small non-coding RNAs that deregulate gene expression at the posttranscriptional level, either by translational repression or by mRNA degradation. These mechanisms may be involved in a complex cascade of cellular events associated with the pathophysiology of many types of cancer. MiRNAs are easily detectable in tissue and blood samples of cancer patients. Therefore, miRNAs hold good promise as potential biomarkers in ovarian cancer. In this review, we attempted to provide a comprehensive profile of key miRNAs involved in ovarian carcinoma to establish mi RNAs as more reliable non-invasive clinical biomarkers for early detection of ovarian cancer compared with protein and DNA biomarkers.展开更多
基金supported by grants from the Medical Scientific Research Foundation and Bureau of Health Care for Senior Officials of Zhejiang Province,China (No.2007B026)the National Natural Science Foundation of China (No.0471819)
文摘OBJECTIVE To identify metastasis-related biomarkers in humanovarian cancer cell lines and in serum.METHODS We isolated total protein from cell lysis solutionsand cultured supernatants from 2 human ovarian cancer cell linesand used SELDI-TOF-MS to detect the differential expressionof the proteins in the 2 cell lines.The proteomic spectra weregenerated using weak cation exchange chips.The biomarkerswere validated by analyzing serum proteins or peptides in ovariancancer patients,relapsed ovarian cancer patients,patients withbenign ovarian tumors,and healthy people.RESULTS Four proteins in the culture supernatant fromHO-8910PM cells were up-regulated,relative to the culturesupernatant of HO-8910 cells.One protein (3,144 Da m/z value)was up-regulated in both the cell lysis solution and in the culturesupernatant of HO-8910PM cells.In addition,expression of the3,144 Da m/z protein differed significantly between serum fromthe 26 ovarian cancer patients,from the 22 relapsed ovarianpatients and from the 37 healthy women (P<0.01).However,therewas no difference between patients with benign ovarian tumorsand healthy people (P>0.5).CONCLUSION Ovarian cancer cell lines with high or lowmetastatic potential have distinct protein profiles.Protein 3,144Da m/z could be a useful biomarker for diagnosing ovarian cancermetastasis.
基金the ICMR New Delhi for financial support (Grant No. 3/2/2/136/2012/NCD-Ⅲ)
文摘Epithelial ovarian cancer(EOC) is the leading cause of death among all gynecological malignancies. Despite the technological and medical advances over the past four decades, such as the development of several biological markers(mRNA and proteins biomarkers), the mortality rate of ovarian cancer remains a challenge because of its late diagnosis, which is specifically attributed to low specificities and sensitivities. Under this compulsive scenario, recent advances in expression biology have shifted in identifying and developing specific and sensitive biomarkers, such as micro RNAs(miRNAs) for cancer diagnosis and prognosis. MiRNAs are a novel class of small non-coding RNAs that deregulate gene expression at the posttranscriptional level, either by translational repression or by mRNA degradation. These mechanisms may be involved in a complex cascade of cellular events associated with the pathophysiology of many types of cancer. MiRNAs are easily detectable in tissue and blood samples of cancer patients. Therefore, miRNAs hold good promise as potential biomarkers in ovarian cancer. In this review, we attempted to provide a comprehensive profile of key miRNAs involved in ovarian carcinoma to establish mi RNAs as more reliable non-invasive clinical biomarkers for early detection of ovarian cancer compared with protein and DNA biomarkers.
