Inflammatory bowel disease(IBD)is a serious disorder,and exploration of active compounds to treat it is necessary.An acidic polysaccharide named SUSP-4 was purified from Selaginella uncinata(Desv.)Spring,which contain...Inflammatory bowel disease(IBD)is a serious disorder,and exploration of active compounds to treat it is necessary.An acidic polysaccharide named SUSP-4 was purified from Selaginella uncinata(Desv.)Spring,which contained galacturonic acid,galactose,xylose,arabinose,and rhamnose with the main chain structure of→4)-α-d-GalAp-(1→and→6)-β-d-Galp-(1→and the branched structure of→5)-α-l-Araf-(1→.Animal experiments showed that compared with Model group,SUSP-4 significantly improved body weight status,disease activity index(DAI),colonic shortening,and histopathological damage,and elevated occludin and zonula occludens protein 1(ZO-1)expression in mice induced by dextran sulfate sodium salt(DSS).16S ribosomal RNA(rRNA)sequencing indicated that SUSP-4 markedly downregulated the level of Akkermansia and Alistipes.Metabolomics results confirmed that SUSP-4 obviously elevated thiamine levels compared with Model mice by adjusting thiamine metabolism,which was further confirmed by a targeted metabolism study.Fecal transplantation experiments showed that SUSP-4 exerted an anti-IBD effect by altering the intestinal flora in mice.A mechanistic study showed that SUSP-4 markedly inhibited macrophage activation by decreasing the levels of phospho-nuclear factor kappa-B(p-NF-κB)and cyclooxygenase-2(COX-2)and elevating NF-E2-related factor 2(Nrf2)levels compared with Model group.In conclusion,SUSP-4 affected thiamine metabolism by regulating Akkermania and inhibited macrophage activation to adjust NF-κB/Nrf2/COX-2-mediated inflammation and oxidative stress against IBD.This is the first time that plant polysaccharides have been shown to affect thiamine metabolism against IBD,showing great potential for in-depth research and development applications.展开更多
卷柏(Selaginella tamariscina)含有丰富的黄酮类成分,以双黄酮为主。利用AB-8大孔树脂对脂溶性成分的吸附作用和聚酰胺树脂对黄酮类成分吸附和解吸的特点,将二者串联对卷柏提取物进行分离纯化,建立垫状卷柏总黄酮(Total flavonoids of ...卷柏(Selaginella tamariscina)含有丰富的黄酮类成分,以双黄酮为主。利用AB-8大孔树脂对脂溶性成分的吸附作用和聚酰胺树脂对黄酮类成分吸附和解吸的特点,将二者串联对卷柏提取物进行分离纯化,建立垫状卷柏总黄酮(Total flavonoids of selaginella pulvinata,TFSP)提取纯化工艺。采用紫外分光光度法测定TFSP含量,并通过MTT法,检测TFSP对神经细胞过氧化氢损伤的保护与修复作用。结果表明,垫状卷柏提取物先通过AB-8大孔树脂吸附脂溶性成分,再经聚酰胺吸附和解吸卷柏总黄酮,在聚酰胺树脂上样量1∶2(卷柏与树脂的体积比)、80%乙醇洗脱5∶1(洗脱液与聚酰胺柱的体积比)等最佳纯化工艺条件下,TFSP的平均含量达81.4%,工艺过程科学合理、简便易行。将TFSP与SH-SY5Y细胞共孵育,可显著减轻神经细胞被过氧化氢损伤的程度,并对已经受损的细胞具有明显的修复作用。TFSP具有为神经退行性疾病开发治疗药物的潜力。展开更多
基金the funding from the Spring City Plan of the High-Level Talent Promotion and Training Project of Kunming,China(Grant No.:2022SCP008)the Independent Research Fund of Yunnan Characteristic Plant Extraction Laboratory,China(Grant No.:2022YKZY001).
文摘Inflammatory bowel disease(IBD)is a serious disorder,and exploration of active compounds to treat it is necessary.An acidic polysaccharide named SUSP-4 was purified from Selaginella uncinata(Desv.)Spring,which contained galacturonic acid,galactose,xylose,arabinose,and rhamnose with the main chain structure of→4)-α-d-GalAp-(1→and→6)-β-d-Galp-(1→and the branched structure of→5)-α-l-Araf-(1→.Animal experiments showed that compared with Model group,SUSP-4 significantly improved body weight status,disease activity index(DAI),colonic shortening,and histopathological damage,and elevated occludin and zonula occludens protein 1(ZO-1)expression in mice induced by dextran sulfate sodium salt(DSS).16S ribosomal RNA(rRNA)sequencing indicated that SUSP-4 markedly downregulated the level of Akkermansia and Alistipes.Metabolomics results confirmed that SUSP-4 obviously elevated thiamine levels compared with Model mice by adjusting thiamine metabolism,which was further confirmed by a targeted metabolism study.Fecal transplantation experiments showed that SUSP-4 exerted an anti-IBD effect by altering the intestinal flora in mice.A mechanistic study showed that SUSP-4 markedly inhibited macrophage activation by decreasing the levels of phospho-nuclear factor kappa-B(p-NF-κB)and cyclooxygenase-2(COX-2)and elevating NF-E2-related factor 2(Nrf2)levels compared with Model group.In conclusion,SUSP-4 affected thiamine metabolism by regulating Akkermania and inhibited macrophage activation to adjust NF-κB/Nrf2/COX-2-mediated inflammation and oxidative stress against IBD.This is the first time that plant polysaccharides have been shown to affect thiamine metabolism against IBD,showing great potential for in-depth research and development applications.
