From beginning of 90＇s, the Brazilian nuclear policy has been changed radically. This determined the interruption of most R＆D fuel cycle activities and the facilities shutdown at Nuclear and Energetic Research Insti...From beginning of 90＇s, the Brazilian nuclear policy has been changed radically. This determined the interruption of most R＆D fuel cycle activities and the facilities shutdown at Nuclear and Energetic Research Institute （IPEN）. The existence of those facilities also implicated in the need of constant surveillance, representing additional obligations, costs and problems. The reasons to promote the dismantling of the IPEN＇s Nuclear Fuel Cycle Pilot Plants elapsed mainly from the need of physical space for new activities, since the R＆D in the nuclear fuel cycle area were interrupted. In the last decade, IPEN has changed its ＂nuclear profile＂ to a ＂comprehensive and multidisciplinary profile＂. With the end of most nuclear fuel cycle activities, the former facilities were distributed in four different centers. Each center has adopted a different strategy and priority to face the D＆D problem. The available resources depend on the specific program in each area＇s development （resources available from other sources, not only from Brazilian National Nuclear Energy Commission （CNEN）. One of those new activities is the IPEN＇s Environmental Program. This paper describes the procedures, problems faced and results related to the reintegration of the former pilot plant areas as new laboratories of the Chemical and Environmental Technology Center-CQMA of the IPEN.展开更多
In this report, the author describes and compares two innovative processes for producing thermal energy based on cavitation and nuclear fusion reactions in the indoor environment. Experiments conducted in the laborato...In this report, the author describes and compares two innovative processes for producing thermal energy based on cavitation and nuclear fusion reactions in the indoor environment. Experiments conducted in the laboratory IHS （Intensive Heating System） of IE ＂Revinov N. M.＂ indicate that the process of lasso-vortex cavitation, which generates by means of an electric arc obtained HV-EI, gas-liquid plasma state in the EHH-CTC （electro-hydraulic heater with cavitation thermal camera） is not inferior and sometimes even superior in heat transfer to NF-AC （nuclear fusion reactions in ambient conditions）.展开更多
The study was conducted to demonstrate the performance of the large-scale commercial plant of the hydrothermal drying of sewage sludge. Its performance was compared with that of the small-scale pilot facility to confi...The study was conducted to demonstrate the performance of the large-scale commercial plant of the hydrothermal drying of sewage sludge. Its performance was compared with that of the small-scale pilot facility to confirm the scaling effect of the facility. It has been shown that the optimum reaction temperature was 190℃ and reaction time was 30 minutes for both facilities. The dehydration performance of the products of the hydrothermal treatment and the natural drying performance of the dehydrated residue were almost the same for both facilities, which demonstrated that the small-scale pilot facility could simulate the commercial-scale plant well. With these optimum hydrothermal reaction conditions, the moisture content of the dehydrated product was less than 40% by the use of the frame filter, which had better dehydration performance than the centrifuge dehydrator. The moisture content of the dehydrated solid residue could be reduced less than 20% and 10% after 24 hours and 48 hours natural drying, respectively. The solubilization rate of sludge into the dehydrated liquid was 10% and 90% of the solid content in the raw sludge could be recovered as a dry solid fuel by this process with reduced energy requirement compared with conventional thermal drying processes. Too high reaction temperature or too long reaction time increased the content of solid material in the dehydrated liquid （solubilization rate of sludge） and reduced the yield of the dry solid fuel.展开更多
Usually the water head of the pumped storage hydro-plant is high, generally up to 400-500 m, therefore the rock mass under the high-pressure bifurcation pipe have to bear as high as millions Pascal water pressure, in ...Usually the water head of the pumped storage hydro-plant is high, generally up to 400-500 m, therefore the rock mass under the high-pressure bifurcation pipe have to bear as high as millions Pascal water pressure, in according with the requirements of high water head pumped storage hydro-plant should be 1.2 times of the water head special high-pressure packer permeability test compared with normal to test the permeability of rock and rock cleavage pressure value. The test results on the choice of design options often play a decisive role. Based on the engineering practice, the authors studied the drillhole high-pressure packer permeability test in the pumped storage hydro-plant's underground powerhouse, by the analysis of test results, this article offers a demonstration of the deformation of rock fracture witch under building in the condition of high-pressure water head, it provides a more detailed engineering geological background.展开更多
Regarding the studies of other authors about energy performance of a reverse osmosis desalination plant operating with variable pressure; about desalination efficiency in their different recent articles, we continued ...Regarding the studies of other authors about energy performance of a reverse osmosis desalination plant operating with variable pressure; about desalination efficiency in their different recent articles, we continued working in this way researching in a real seawater reverse osmosis plant following the items below. The objectives of this test are to get the minimum electrical consumption and energy costs for the operation of a sea water desalination plant using membranes. We need to compare the performance of the different membranes manufacturer＇s and to determine, under this new thermal scenario, the optimal configuration to get the water quality and quantity needs with the minimum energy consumption.展开更多
This study, carried out in a sugarcane mill located in the center-west region of Sao Paulo state, Brazil, aimed to evaluate the association of sucrose amount （POL%） with sugarcane varieties and cutting stages using ...This study, carried out in a sugarcane mill located in the center-west region of Sao Paulo state, Brazil, aimed to evaluate the association of sucrose amount （POL%） with sugarcane varieties and cutting stages using mechanized harvesting. A two-way analysis of variance （ANOVA）, complemented by multiple comparison tests, was used in order to identify the effects of variety types and cutting stages on the sugarcane POL% content. An interaction between the sugarcane variety and the cutting stage was observed; therefore, both two factors cannot be independently evaluated regarding POL%. In addition, it was found that the sucrose amount from the plant production in the evaluated period presented significant differences between the cutting stages only for one variety, namely CTC2, which was different from the others in most of the cutting stages. Considering the observed results, it can be concluded that analyzing sucrose amount statistically in the mill production can allow producers to posteriorly better monitor information on the sugarcane varieties harvested under mechanized processes.展开更多
The sipping test devices are used to identify the defective fuel. The defective fuel can be identified by detecting the occurrence of the fission products that are entrained by the medium rising around the fuel rods. ...The sipping test devices are used to identify the defective fuel. The defective fuel can be identified by detecting the occurrence of the fission products that are entrained by the medium rising around the fuel rods. This project is the setting of the two kinds of the three sipping test devices to inspect the tightness of the irradiated fuel assembly from two units NPP （nuclear power plant）. The in-mast sipping shared by two units is used for qualitative tightness test of each fuel assembly during refueling operation above the reactor. The two poolside sipping is used for quantitatively confirming the diagnosis of the in-mast sipping and identifying the tightness of the fuel at the side of the fuel storage pools of each unit after refueling. The project was implemented by research, design manufacture and calibration of these three devices and completed successfully with serious quality assurance and quality control. The performance of these devices is well demonstrated.展开更多
Ammonia corrosion in copper tube will affect the safety of boiler running in power plant. Therefore, no copper in heating system has become a technical orientation in heat exchanger reconstruction, This paper analy...Ammonia corrosion in copper tube will affect the safety of boiler running in power plant. Therefore, no copper in heating system has become a technical orientation in heat exchanger reconstruction, This paper analyzes the condition and mechanism of ammonia corrosion occurring in copper tube used in coal-fired power plants. Using a general steam condensation testing equipment only for horizontal single tube, with water vapor and water as working fluid, on two types of steel tube with 2-side enhancement heat transfer, namely, a spirally fluted tube and a ratchet tube with internal spiral groove （RISG tube） which was developed recently, a set of experimental tests are conducted to investigate the characteristics of heat transfer and hydromeehanics. In order to compare easily, both one copper smooth tube and one steel smooth tube are also used in the experiment. The experimental results, which get from single horizontal tube, show that the overall heat transfer coefficient of steel spirally fluted tube are improved by 10%o to 17%, and that of the steel RISG tube（22%-28%） is better than steel spirally fluted tube, its flow resistance coefficient is only increased by 22% to 66% when compared with smooth tube. Based on a lot of experimental data, the steel spirally fluted tube and the steel RISG tube were applied in a low pressure preheater and an oil-cooler of some or other power plant respectively. The field testing results showed that their heat transfer coefficient with each types of enhancement heat transfer tubes were improved by 2.5% and 21%-45% comparing with copper smooth tube heat exchangers. Both basic and field experiment indicates that the steel tube with 2-side enhancement heat transfer is an ideal choice for heat exchanger reconstruction in no copper issue in power plants.展开更多
This study sought to assess Gweru＇s water supply system from the source to the consumer to find out whether the water is safe for domestic purposes, mainly consumption. Water samples were taken from Gwenoro dam （raw...This study sought to assess Gweru＇s water supply system from the source to the consumer to find out whether the water is safe for domestic purposes, mainly consumption. Water samples were taken from Gwenoro dam （raw water）, Gwenoro treatment plant （treated water） as well as from GIS-generated random points of residential areas closest to Gwenoro and furthest from both the dam and the treatment plant. These were taken for laboratory testing where parameters like pH, hardness, turbidity and DO （dissolved oxygen） were tested. Questionnaires were used to solicit the views of the consumers on water quality and water service delivery from eight randomly selected residential suburbs. Interviews were also administered to Gwenoro Water Treatment Plant superintendent and deputy to get insight into the day to day challenges that they have to grapple with. Results generally show that treated water from Gwenoro treatment plant is relatively safe to drink for those who have access to it. However, some consumer points like Mkoba 13 and Mkoba 18 receive water whose DO is out of the recommended range. Suburbs which lie on higher ground like Mkoba 19 and Mkoba 14 however rarely receive water from Gwenoro with some residents of Mkoba 19 having had no water from their taps for more than three years now. The study recommends that new equipment be bought for the treatment plant so that treated water reaches all parts of the city. Newly resettled farmers in the Upper Runde Catchment must be resettled elsewhere while urban stream bank cultivation must be banned to reduce water pollution as well as possible sedimentation of Gwenoro dam.展开更多
The load-bearing characters of hydraulic-powered roof support with dual telescopic legs were analyzed. With a specific type hydraulic-powered roof support with dual telescopic legs for research object, the inside load...The load-bearing characters of hydraulic-powered roof support with dual telescopic legs were analyzed. With a specific type hydraulic-powered roof support with dual telescopic legs for research object, the inside load test problems in factories was analyzed, and the correct test methods were given, which can enhance the test efficiency and make the factories away from the error design of hydraulic-powered roof supports and legs.展开更多
The electric energy which is generated by wind power plants depends on the wind speed and exceeds with strong permissible wind speed the electric energy requirements of the country. In order not to reduce this electri...The electric energy which is generated by wind power plants depends on the wind speed and exceeds with strong permissible wind speed the electric energy requirements of the country. In order not to reduce this electrical energy, it must be stored. The sensible energy storage is currently the pumped storage power plants. As the mountain ranges for conventional pumped storage power plants with drop heights of H 〉 600 m are strictly limited, the development of low potential pumped storage power plants has begun. Increasing the capacity of pumped storage power plants with regard to the wind power plants is urgently needed. In this paper, it is shown using the example of an unneeded port facility, how a port facility can be used after low conversion as a test facility for low potential pumped storage power plants and at the same time for the testing of hydro-kinetic turbines. This type of pump storage power plants does not save the energy due to large drop heights, but primarily due to the large volume flow of water.展开更多
The research object was high 120 m concrete gravity dam of the Angara Bratsk hydroelectric power plant. The state of the concrete dam is estimated based on the results of continuous supervision performed by site perso...The research object was high 120 m concrete gravity dam of the Angara Bratsk hydroelectric power plant. The state of the concrete dam is estimated based on the results of continuous supervision performed by site personnel as well as periodic monitoring. According to the classification of the interrelations in the system ＂concrete-environment＂, there were selected some important parts of dams and a comprehensive analysis of concrete was executed on these parts. Concerning the complex research of concrete, a combination of full-scale tests with core-sampling has been proposed. Core samples tests had an object to study the deep concrete layers and to determine the specific indicators such as strength, density, porosity, comparative diameter of capillary pores, CaO content in cement stone and others. Obtained findings and recommended criteria can be applied when selecting technologies for constructing dams that guarantee their durability in the north.展开更多
The spatial distribution of extracellular polymeric substances （EPS） in a pilot-scale membrane bioreactor （MBR） was studied. The sampling points on top of and inside the membrane module were measured and analyzed ...The spatial distribution of extracellular polymeric substances （EPS） in a pilot-scale membrane bioreactor （MBR） was studied. The sampling points on top of and inside the membrane module were measured and analyzed by the experimental variant function. The content of EPS was spatially interpolated by ordinary Kriging method, and il- lustrated with SURFER software. A case study was carried out in an MBR with membrane aperture of 0.4 ~tm and handling capacity of 120 ma/d in Jizhuangzi sewage treatment plant, Tianjin. From the visualization of EPS distribu- tion, it is seen that on the horizontal plane, the content of EPS was the lowest at the center; and on the vertical plane, the content of EPS decreased with the increase of depth. The shearing force caused by aeration of perforated pipe and the influent mode are the main influencing factors for this distribution.展开更多
Synthetic biology is an interdisciplinary field that takes top-down approaches to understand and engineer biological systems through design-build-test cycles. A number of advances in this relatively young field have g...Synthetic biology is an interdisciplinary field that takes top-down approaches to understand and engineer biological systems through design-build-test cycles. A number of advances in this relatively young field have greatly accelerated such engineering cycles. Specifically, various innovative tools were developed for in silico biosystems design, DNA de novo synthesis and assembly, construct verification, as well as metabolite analysis, which have laid a solid foundation for building biological foundries for rapid prototyping of improved or novel biosystems. This review summarizes the state-of-the-art technologies for synthetic biology and discusses the challenges to establish such biological foundries.展开更多
文摘From beginning of 90＇s, the Brazilian nuclear policy has been changed radically. This determined the interruption of most R＆D fuel cycle activities and the facilities shutdown at Nuclear and Energetic Research Institute （IPEN）. The existence of those facilities also implicated in the need of constant surveillance, representing additional obligations, costs and problems. The reasons to promote the dismantling of the IPEN＇s Nuclear Fuel Cycle Pilot Plants elapsed mainly from the need of physical space for new activities, since the R＆D in the nuclear fuel cycle area were interrupted. In the last decade, IPEN has changed its ＂nuclear profile＂ to a ＂comprehensive and multidisciplinary profile＂. With the end of most nuclear fuel cycle activities, the former facilities were distributed in four different centers. Each center has adopted a different strategy and priority to face the D＆D problem. The available resources depend on the specific program in each area＇s development （resources available from other sources, not only from Brazilian National Nuclear Energy Commission （CNEN）. One of those new activities is the IPEN＇s Environmental Program. This paper describes the procedures, problems faced and results related to the reintegration of the former pilot plant areas as new laboratories of the Chemical and Environmental Technology Center-CQMA of the IPEN.
文摘In this report, the author describes and compares two innovative processes for producing thermal energy based on cavitation and nuclear fusion reactions in the indoor environment. Experiments conducted in the laboratory IHS （Intensive Heating System） of IE ＂Revinov N. M.＂ indicate that the process of lasso-vortex cavitation, which generates by means of an electric arc obtained HV-EI, gas-liquid plasma state in the EHH-CTC （electro-hydraulic heater with cavitation thermal camera） is not inferior and sometimes even superior in heat transfer to NF-AC （nuclear fusion reactions in ambient conditions）.
文摘The study was conducted to demonstrate the performance of the large-scale commercial plant of the hydrothermal drying of sewage sludge. Its performance was compared with that of the small-scale pilot facility to confirm the scaling effect of the facility. It has been shown that the optimum reaction temperature was 190℃ and reaction time was 30 minutes for both facilities. The dehydration performance of the products of the hydrothermal treatment and the natural drying performance of the dehydrated residue were almost the same for both facilities, which demonstrated that the small-scale pilot facility could simulate the commercial-scale plant well. With these optimum hydrothermal reaction conditions, the moisture content of the dehydrated product was less than 40% by the use of the frame filter, which had better dehydration performance than the centrifuge dehydrator. The moisture content of the dehydrated solid residue could be reduced less than 20% and 10% after 24 hours and 48 hours natural drying, respectively. The solubilization rate of sludge into the dehydrated liquid was 10% and 90% of the solid content in the raw sludge could be recovered as a dry solid fuel by this process with reduced energy requirement compared with conventional thermal drying processes. Too high reaction temperature or too long reaction time increased the content of solid material in the dehydrated liquid （solubilization rate of sludge） and reduced the yield of the dry solid fuel.
文摘Usually the water head of the pumped storage hydro-plant is high, generally up to 400-500 m, therefore the rock mass under the high-pressure bifurcation pipe have to bear as high as millions Pascal water pressure, in according with the requirements of high water head pumped storage hydro-plant should be 1.2 times of the water head special high-pressure packer permeability test compared with normal to test the permeability of rock and rock cleavage pressure value. The test results on the choice of design options often play a decisive role. Based on the engineering practice, the authors studied the drillhole high-pressure packer permeability test in the pumped storage hydro-plant's underground powerhouse, by the analysis of test results, this article offers a demonstration of the deformation of rock fracture witch under building in the condition of high-pressure water head, it provides a more detailed engineering geological background.
文摘Regarding the studies of other authors about energy performance of a reverse osmosis desalination plant operating with variable pressure; about desalination efficiency in their different recent articles, we continued working in this way researching in a real seawater reverse osmosis plant following the items below. The objectives of this test are to get the minimum electrical consumption and energy costs for the operation of a sea water desalination plant using membranes. We need to compare the performance of the different membranes manufacturer＇s and to determine, under this new thermal scenario, the optimal configuration to get the water quality and quantity needs with the minimum energy consumption.
文摘This study, carried out in a sugarcane mill located in the center-west region of Sao Paulo state, Brazil, aimed to evaluate the association of sucrose amount （POL%） with sugarcane varieties and cutting stages using mechanized harvesting. A two-way analysis of variance （ANOVA）, complemented by multiple comparison tests, was used in order to identify the effects of variety types and cutting stages on the sugarcane POL% content. An interaction between the sugarcane variety and the cutting stage was observed; therefore, both two factors cannot be independently evaluated regarding POL%. In addition, it was found that the sucrose amount from the plant production in the evaluated period presented significant differences between the cutting stages only for one variety, namely CTC2, which was different from the others in most of the cutting stages. Considering the observed results, it can be concluded that analyzing sucrose amount statistically in the mill production can allow producers to posteriorly better monitor information on the sugarcane varieties harvested under mechanized processes.
文摘The sipping test devices are used to identify the defective fuel. The defective fuel can be identified by detecting the occurrence of the fission products that are entrained by the medium rising around the fuel rods. This project is the setting of the two kinds of the three sipping test devices to inspect the tightness of the irradiated fuel assembly from two units NPP （nuclear power plant）. The in-mast sipping shared by two units is used for qualitative tightness test of each fuel assembly during refueling operation above the reactor. The two poolside sipping is used for quantitatively confirming the diagnosis of the in-mast sipping and identifying the tightness of the fuel at the side of the fuel storage pools of each unit after refueling. The project was implemented by research, design manufacture and calibration of these three devices and completed successfully with serious quality assurance and quality control. The performance of these devices is well demonstrated.
文摘Ammonia corrosion in copper tube will affect the safety of boiler running in power plant. Therefore, no copper in heating system has become a technical orientation in heat exchanger reconstruction, This paper analyzes the condition and mechanism of ammonia corrosion occurring in copper tube used in coal-fired power plants. Using a general steam condensation testing equipment only for horizontal single tube, with water vapor and water as working fluid, on two types of steel tube with 2-side enhancement heat transfer, namely, a spirally fluted tube and a ratchet tube with internal spiral groove （RISG tube） which was developed recently, a set of experimental tests are conducted to investigate the characteristics of heat transfer and hydromeehanics. In order to compare easily, both one copper smooth tube and one steel smooth tube are also used in the experiment. The experimental results, which get from single horizontal tube, show that the overall heat transfer coefficient of steel spirally fluted tube are improved by 10%o to 17%, and that of the steel RISG tube（22%-28%） is better than steel spirally fluted tube, its flow resistance coefficient is only increased by 22% to 66% when compared with smooth tube. Based on a lot of experimental data, the steel spirally fluted tube and the steel RISG tube were applied in a low pressure preheater and an oil-cooler of some or other power plant respectively. The field testing results showed that their heat transfer coefficient with each types of enhancement heat transfer tubes were improved by 2.5% and 21%-45% comparing with copper smooth tube heat exchangers. Both basic and field experiment indicates that the steel tube with 2-side enhancement heat transfer is an ideal choice for heat exchanger reconstruction in no copper issue in power plants.
文摘This study sought to assess Gweru＇s water supply system from the source to the consumer to find out whether the water is safe for domestic purposes, mainly consumption. Water samples were taken from Gwenoro dam （raw water）, Gwenoro treatment plant （treated water） as well as from GIS-generated random points of residential areas closest to Gwenoro and furthest from both the dam and the treatment plant. These were taken for laboratory testing where parameters like pH, hardness, turbidity and DO （dissolved oxygen） were tested. Questionnaires were used to solicit the views of the consumers on water quality and water service delivery from eight randomly selected residential suburbs. Interviews were also administered to Gwenoro Water Treatment Plant superintendent and deputy to get insight into the day to day challenges that they have to grapple with. Results generally show that treated water from Gwenoro treatment plant is relatively safe to drink for those who have access to it. However, some consumer points like Mkoba 13 and Mkoba 18 receive water whose DO is out of the recommended range. Suburbs which lie on higher ground like Mkoba 19 and Mkoba 14 however rarely receive water from Gwenoro with some residents of Mkoba 19 having had no water from their taps for more than three years now. The study recommends that new equipment be bought for the treatment plant so that treated water reaches all parts of the city. Newly resettled farmers in the Upper Runde Catchment must be resettled elsewhere while urban stream bank cultivation must be banned to reduce water pollution as well as possible sedimentation of Gwenoro dam.
文摘The load-bearing characters of hydraulic-powered roof support with dual telescopic legs were analyzed. With a specific type hydraulic-powered roof support with dual telescopic legs for research object, the inside load test problems in factories was analyzed, and the correct test methods were given, which can enhance the test efficiency and make the factories away from the error design of hydraulic-powered roof supports and legs.
文摘The electric energy which is generated by wind power plants depends on the wind speed and exceeds with strong permissible wind speed the electric energy requirements of the country. In order not to reduce this electrical energy, it must be stored. The sensible energy storage is currently the pumped storage power plants. As the mountain ranges for conventional pumped storage power plants with drop heights of H 〉 600 m are strictly limited, the development of low potential pumped storage power plants has begun. Increasing the capacity of pumped storage power plants with regard to the wind power plants is urgently needed. In this paper, it is shown using the example of an unneeded port facility, how a port facility can be used after low conversion as a test facility for low potential pumped storage power plants and at the same time for the testing of hydro-kinetic turbines. This type of pump storage power plants does not save the energy due to large drop heights, but primarily due to the large volume flow of water.
文摘The research object was high 120 m concrete gravity dam of the Angara Bratsk hydroelectric power plant. The state of the concrete dam is estimated based on the results of continuous supervision performed by site personnel as well as periodic monitoring. According to the classification of the interrelations in the system ＂concrete-environment＂, there were selected some important parts of dams and a comprehensive analysis of concrete was executed on these parts. Concerning the complex research of concrete, a combination of full-scale tests with core-sampling has been proposed. Core samples tests had an object to study the deep concrete layers and to determine the specific indicators such as strength, density, porosity, comparative diameter of capillary pores, CaO content in cement stone and others. Obtained findings and recommended criteria can be applied when selecting technologies for constructing dams that guarantee their durability in the north.
基金Supported by Special Fund Project for Technology Innovation of Tianjin (No.06FZZDSH00900)
文摘The spatial distribution of extracellular polymeric substances （EPS） in a pilot-scale membrane bioreactor （MBR） was studied. The sampling points on top of and inside the membrane module were measured and analyzed by the experimental variant function. The content of EPS was spatially interpolated by ordinary Kriging method, and il- lustrated with SURFER software. A case study was carried out in an MBR with membrane aperture of 0.4 ~tm and handling capacity of 120 ma/d in Jizhuangzi sewage treatment plant, Tianjin. From the visualization of EPS distribu- tion, it is seen that on the horizontal plane, the content of EPS was the lowest at the center; and on the vertical plane, the content of EPS decreased with the increase of depth. The shearing force caused by aeration of perforated pipe and the influent mode are the main influencing factors for this distribution.
基金the National Institutes of Health(GM077596)the National Academies Keck Futures Initiative on Synthetic Biology,Defense Advanced Research Program Agency,Roy J.Carver Charitable TrustInstitute for Genomic Biology at the University of Illinois at Urbana-Champaign for financial support in our development and application of DNA assembly technologies
文摘Synthetic biology is an interdisciplinary field that takes top-down approaches to understand and engineer biological systems through design-build-test cycles. A number of advances in this relatively young field have greatly accelerated such engineering cycles. Specifically, various innovative tools were developed for in silico biosystems design, DNA de novo synthesis and assembly, construct verification, as well as metabolite analysis, which have laid a solid foundation for building biological foundries for rapid prototyping of improved or novel biosystems. This review summarizes the state-of-the-art technologies for synthetic biology and discusses the challenges to establish such biological foundries.
