目的 本研究旨在探讨细胞外基质刚度变化对神经干细胞(neural stem cells,NSCs)分化的影响及其作用机制。方法 本研究基于成功构建脊髓损伤大鼠模型,并制备不同刚度(0.7 k Pa、40 k Pa)的聚丙烯酰胺凝胶基底,将大鼠原代NSCs于不同刚度...目的 本研究旨在探讨细胞外基质刚度变化对神经干细胞(neural stem cells,NSCs)分化的影响及其作用机制。方法 本研究基于成功构建脊髓损伤大鼠模型,并制备不同刚度(0.7 k Pa、40 k Pa)的聚丙烯酰胺凝胶基底,将大鼠原代NSCs于不同刚度基底上培养。压电型机械敏感离子通道组件1 (piezo type mechanosensitive ion channel component 1,Piezo1) sh RNA质粒转染NSCs细胞。免疫荧光染色检测神经元标志物双皮质醇(doublecortion,DCX)和星形胶质细胞标志物胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)阳性细胞百分比。免疫组织化学及蛋白质免疫印迹(Western blot)法检测损伤组织及NSCs细胞中Piezo1蛋白的表达水平。结果 与0.7 k Pa基质刚度组相比,40 k Pa基质刚度组中DCX阳性细胞数增加,而GFAP阳性细胞数减少,Piezo1蛋白表达量上升。脊髓损伤大鼠损伤组织Piezo1蛋白表达显著高于空白对照(sham)组。40 k Pa基质刚度条件下沉默Piezo1后,DCX阳性细胞数减少,而GFAP阳性细胞数增加,差异具有统计学意义(P<0.05)。机制研究发现,沉默Piezo1导致IV型胶原及纤连蛋白表达下降。重组纤连蛋白逆转了Piezo1 sh RNA对NSCs分化的影响,即DCX阳性细胞数增加,而GFAP阳性细胞数减少。结论 综上可见,硬基底刚度通过促进Piezo1蛋白表达,上调IV型胶原及纤连蛋白表达,从而调控NSCs细胞分化。本研究为基于生物材料治疗脊髓损伤提供了新的视角。展开更多
This paper proposes a voltage-based hot-spot detection method for defective cells in PV module using projector. The presence of internal crystal defects is one of the main causes of hot-spot phenomenon in PV modules. ...This paper proposes a voltage-based hot-spot detection method for defective cells in PV module using projector. The presence of internal crystal defects is one of the main causes of hot-spot phenomenon in PV modules. Authors previously investigated the physical characteristics of hot-spot phenomenon referring to internal crystal defect. Based on it, a hot-spot detection method named as current-based SRC （self reverse current） detection method is developed. However, it becomes extraordinarily complicated to determine the defective cells under low illumination. In order to avoid this disadvantage, authors improve the SRC detection method by applying voltage. From the feasibility experiment results, it is confirmed that by calculating cell HSI （hotspots index） with voltage, the PV modules with defective cells can be prospectively excluded even under low illumination.展开更多
HPR1000 is an advanced nuclear power plant(NPP)with the significant feature of an active and passive safety design philosophy,developed by the China National Nuclear Corporation.On one hand,it is an evolutionary desig...HPR1000 is an advanced nuclear power plant(NPP)with the significant feature of an active and passive safety design philosophy,developed by the China National Nuclear Corporation.On one hand,it is an evolutionary design based on proven technology of the existing pressurized water reactor NPP;on the other hand,it incorporates advanced design features including a 177-fuel-assembly core loaded with CF3 fuel assemblies,active and passive safety systems,comprehensive severe accident prevention and mitigation measures,enhanced protection against external events,and improved emergency response capability.Extensive verification experiments and tests have been performed for critical innovative improvements on passive systems,the reactor core,and the main equipment.The design of HPR1000fulfills the international utility requirements for advanced light water reactors and the latest nuclear safety requirements,and addresses the safety issues relevant to the Fukushima accident.Along with its outstanding safety and economy,HPR1000 provides an excellent and practicable solution for both domestic and international nuclear power markets.展开更多
According to performance analysis of a three-phase grid-connected inverter mathematical model of a directly-driven wind turbine with a permanent magnet synchronous generator (D-PMSG) under unbalanced network voltage c...According to performance analysis of a three-phase grid-connected inverter mathematical model of a directly-driven wind turbine with a permanent magnet synchronous generator (D-PMSG) under unbalanced network voltage conditions, a dual current-loop control strategy (DCC) oriented on positive voltage and negative current is proposed to inhibit the DC voltage fluctuation. Meanwhile, a notch filter is introduced into the conventional control strategy of a phase-locked loop to complete the low voltage ride through (LVRT) ability of the wind generator. A 1.5-MW D-PMSG with a back-to-back IGBT frequency converter was simulated in the PSCAD/EMTDC environment, and simulation results showed that: the maximum wind power tracking was achieved in this system and the proposed DCC strategy could successfully inhibit the rising aging of DC voltage and enhance the ride-through capability of D-PMSG wind generation system under unbalanced network voltage conditions.展开更多
文摘目的 本研究旨在探讨细胞外基质刚度变化对神经干细胞(neural stem cells,NSCs)分化的影响及其作用机制。方法 本研究基于成功构建脊髓损伤大鼠模型,并制备不同刚度(0.7 k Pa、40 k Pa)的聚丙烯酰胺凝胶基底,将大鼠原代NSCs于不同刚度基底上培养。压电型机械敏感离子通道组件1 (piezo type mechanosensitive ion channel component 1,Piezo1) sh RNA质粒转染NSCs细胞。免疫荧光染色检测神经元标志物双皮质醇(doublecortion,DCX)和星形胶质细胞标志物胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)阳性细胞百分比。免疫组织化学及蛋白质免疫印迹(Western blot)法检测损伤组织及NSCs细胞中Piezo1蛋白的表达水平。结果 与0.7 k Pa基质刚度组相比,40 k Pa基质刚度组中DCX阳性细胞数增加,而GFAP阳性细胞数减少,Piezo1蛋白表达量上升。脊髓损伤大鼠损伤组织Piezo1蛋白表达显著高于空白对照(sham)组。40 k Pa基质刚度条件下沉默Piezo1后,DCX阳性细胞数减少,而GFAP阳性细胞数增加,差异具有统计学意义(P<0.05)。机制研究发现,沉默Piezo1导致IV型胶原及纤连蛋白表达下降。重组纤连蛋白逆转了Piezo1 sh RNA对NSCs分化的影响,即DCX阳性细胞数增加,而GFAP阳性细胞数减少。结论 综上可见,硬基底刚度通过促进Piezo1蛋白表达,上调IV型胶原及纤连蛋白表达,从而调控NSCs细胞分化。本研究为基于生物材料治疗脊髓损伤提供了新的视角。
文摘This paper proposes a voltage-based hot-spot detection method for defective cells in PV module using projector. The presence of internal crystal defects is one of the main causes of hot-spot phenomenon in PV modules. Authors previously investigated the physical characteristics of hot-spot phenomenon referring to internal crystal defect. Based on it, a hot-spot detection method named as current-based SRC （self reverse current） detection method is developed. However, it becomes extraordinarily complicated to determine the defective cells under low illumination. In order to avoid this disadvantage, authors improve the SRC detection method by applying voltage. From the feasibility experiment results, it is confirmed that by calculating cell HSI （hotspots index） with voltage, the PV modules with defective cells can be prospectively excluded even under low illumination.
文摘HPR1000 is an advanced nuclear power plant(NPP)with the significant feature of an active and passive safety design philosophy,developed by the China National Nuclear Corporation.On one hand,it is an evolutionary design based on proven technology of the existing pressurized water reactor NPP;on the other hand,it incorporates advanced design features including a 177-fuel-assembly core loaded with CF3 fuel assemblies,active and passive safety systems,comprehensive severe accident prevention and mitigation measures,enhanced protection against external events,and improved emergency response capability.Extensive verification experiments and tests have been performed for critical innovative improvements on passive systems,the reactor core,and the main equipment.The design of HPR1000fulfills the international utility requirements for advanced light water reactors and the latest nuclear safety requirements,and addresses the safety issues relevant to the Fukushima accident.Along with its outstanding safety and economy,HPR1000 provides an excellent and practicable solution for both domestic and international nuclear power markets.
文摘According to performance analysis of a three-phase grid-connected inverter mathematical model of a directly-driven wind turbine with a permanent magnet synchronous generator (D-PMSG) under unbalanced network voltage conditions, a dual current-loop control strategy (DCC) oriented on positive voltage and negative current is proposed to inhibit the DC voltage fluctuation. Meanwhile, a notch filter is introduced into the conventional control strategy of a phase-locked loop to complete the low voltage ride through (LVRT) ability of the wind generator. A 1.5-MW D-PMSG with a back-to-back IGBT frequency converter was simulated in the PSCAD/EMTDC environment, and simulation results showed that: the maximum wind power tracking was achieved in this system and the proposed DCC strategy could successfully inhibit the rising aging of DC voltage and enhance the ride-through capability of D-PMSG wind generation system under unbalanced network voltage conditions.
