[Objective] The aim is to develop the piezoelectric immunosensor to detect H9-subtype avian influenza virus（AIV）.[Method] The immunosensor chip was constructed by self-assembling mercaptopmpionic acid（MPA） to be m...[Objective] The aim is to develop the piezoelectric immunosensor to detect H9-subtype avian influenza virus（AIV）.[Method] The immunosensor chip was constructed by self-assembling mercaptopmpionic acid（MPA） to be monolayer on the silver-coated electrode of quartz crystal and coupling the monoclonal antibody to H9 subtype AIV with N-ethy-N＇-（3-dimethyl aminopropyl）carbodiimide hydrochloride（EDC） and N-hydroxysuccinimide（NHS）.The immunosensor to detect H9 subtype AIV was established.[Result] The results showed that the immunosensor displayed better specificity to H9 AIV and had no response to H5AIV and NDV when it was used for detection.The sensitivity test indicated the detection sensitivity for the H9 subtype AIV could reach 20-100 EID50.[Conclusion] The research provided a foundation for further research on the immunosensor for detecting AIV and it could be a new approach to detect other related viruses.展开更多
The performance of a Venturi tube used in wet gas flow have been explored mainly under higher-pressure condition, but very often, low-pressure test exists in some oil and gas fields in Tianjin Dagang Oil and Gas Field...The performance of a Venturi tube used in wet gas flow have been explored mainly under higher-pressure condition, but very often, low-pressure test exists in some oil and gas fields in Tianjin Dagang Oil and Gas Field in China. In this study, the performance of horizontally mounted Venturi meters in low-pressure wet gas flow is discussed. Three 50 mm Venturi meters were tested systematically, with fl values of 0.4048, 0.55 and 0.70, the opera- tion pressure of 0.15 MPa, 0.20 MPa, 0.25 MPa, the gas densiometric Froude number from 0.6 to 2.0, the modified Lockhart-Maretinelli parameter from 0.0022 to 0.06, and the ratio of the gas liquid mass flow rate from 0.5 to 0.99. The effects of modified Lockhart-Maretinelli parameter, pressure, gas densiometric Froude number, diameter ratio, and gas-liquid mass flow rate ratio to the Venturi tube are analyzed with new independent data. Furthermore, low-pressure performance was compared with that under high pressure.展开更多
The three-dimensional damage constitutive relationship of coal is established and distribution law of the abutment pressure of the integrated coal beside the road-in packing for gob-side entry retaining in fully-mecha...The three-dimensional damage constitutive relationship of coal is established and distribution law of the abutment pressure of the integrated coal beside the road-in packing for gob-side entry retaining in fully-mechanized caving face under the effect of given deformation of the main roof is analyzed by the damage mechanics theory. And the relationship between distribution of the abutment pressure and thickness of coal seam is explored. The presented result is of great theoretical significance and practical value to the study on stability control of the surrounding rock of road-in packing for gob-side entry retaining in fully-mechanized caving face.展开更多
基金Supported by the Supporting Program of the"Eleventh Five-year Plan"for Sci&Tech Research of China(2006BAK20A29)Strategical Project for Science and Technology of Guangdong Province(2004A2090102)~~
文摘[Objective] The aim is to develop the piezoelectric immunosensor to detect H9-subtype avian influenza virus（AIV）.[Method] The immunosensor chip was constructed by self-assembling mercaptopmpionic acid（MPA） to be monolayer on the silver-coated electrode of quartz crystal and coupling the monoclonal antibody to H9 subtype AIV with N-ethy-N＇-（3-dimethyl aminopropyl）carbodiimide hydrochloride（EDC） and N-hydroxysuccinimide（NHS）.The immunosensor to detect H9 subtype AIV was established.[Result] The results showed that the immunosensor displayed better specificity to H9 AIV and had no response to H5AIV and NDV when it was used for detection.The sensitivity test indicated the detection sensitivity for the H9 subtype AIV could reach 20-100 EID50.[Conclusion] The research provided a foundation for further research on the immunosensor for detecting AIV and it could be a new approach to detect other related viruses.
基金the National High Technology Research and Development Program of China(2006AA04Z167,2007AA04Z180)
文摘The performance of a Venturi tube used in wet gas flow have been explored mainly under higher-pressure condition, but very often, low-pressure test exists in some oil and gas fields in Tianjin Dagang Oil and Gas Field in China. In this study, the performance of horizontally mounted Venturi meters in low-pressure wet gas flow is discussed. Three 50 mm Venturi meters were tested systematically, with fl values of 0.4048, 0.55 and 0.70, the opera- tion pressure of 0.15 MPa, 0.20 MPa, 0.25 MPa, the gas densiometric Froude number from 0.6 to 2.0, the modified Lockhart-Maretinelli parameter from 0.0022 to 0.06, and the ratio of the gas liquid mass flow rate from 0.5 to 0.99. The effects of modified Lockhart-Maretinelli parameter, pressure, gas densiometric Froude number, diameter ratio, and gas-liquid mass flow rate ratio to the Venturi tube are analyzed with new independent data. Furthermore, low-pressure performance was compared with that under high pressure.
基金Supported by the National Science Foundation of China (50874042, 50674046)National Science Important Foundation (50634050)Hunan Science Foundation (06JJ50092)
文摘The three-dimensional damage constitutive relationship of coal is established and distribution law of the abutment pressure of the integrated coal beside the road-in packing for gob-side entry retaining in fully-mechanized caving face under the effect of given deformation of the main roof is analyzed by the damage mechanics theory. And the relationship between distribution of the abutment pressure and thickness of coal seam is explored. The presented result is of great theoretical significance and practical value to the study on stability control of the surrounding rock of road-in packing for gob-side entry retaining in fully-mechanized caving face.
