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人胸腺组织原代培养细胞与人类免疫缺陷病毒间的相互作用
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作者 高楠竣 陈英杰 +2 位作者 李雪涛 孔维云 周轶平 《中国组织工程研究与临床康复》 CAS CSCD 北大核心 2011年第50期9344-9347,共4页
背景:人类免疫缺陷病毒能够结合人胸腺的细胞CD4受体,从而导致CD4细胞活性降低。目的:观察人胚胎胸腺组织原代培养与人类免疫缺陷病毒相互作用关系。方法:取人胎胸腺组织进行原代细胞培养,取胸腺的细胞与人类免疫缺陷病毒1ⅢB混合培养... 背景:人类免疫缺陷病毒能够结合人胸腺的细胞CD4受体,从而导致CD4细胞活性降低。目的:观察人胚胎胸腺组织原代培养与人类免疫缺陷病毒相互作用关系。方法:取人胎胸腺组织进行原代细胞培养,取胸腺的细胞与人类免疫缺陷病毒1ⅢB混合培养设为实验组,设置不加人类免疫缺陷病毒培养的胸腺的细胞为对照组。结果与结论:透射电镜观察显示,培养40h,人类免疫缺陷病毒诱导的胸腺的细胞内即可发现大量艾滋病病毒颗粒。MTT法和免疫组织化学染色检测结果显示,培养40h^22d,HIV诱导胸腺的细胞吸光度值均显著低于对照组(r=0.9733,P<0.05),人类免疫缺陷病毒诱导胸腺的细胞膜上和细胞浆Fas-L阳性表达率均升高(P<0.05)。说明人类免疫缺陷病毒可致原代培养的人胚胎胸腺的细胞活性降低。 展开更多
关键词 人类免疫缺陷病毒 胸腺 代培:细胞活性 组织构建
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Effect of lead on ERK activity and the protective function of bFGF in rat primary culture astroglia 被引量:3
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作者 ZHANG Ying YE Li-ping WANG Biao CAO Shi-cheng SUN Li-guang 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2007年第6期422-427,共6页
Objective: To observe the effects of lead on levels ofphosphorylated extracellular signal regulated kinase (p-ERK) in the cytoplasm of primary cultures of rat astroglial cells and the possible protective effect of ... Objective: To observe the effects of lead on levels ofphosphorylated extracellular signal regulated kinase (p-ERK) in the cytoplasm of primary cultures of rat astroglial cells and the possible protective effect of basic fibroblast growth factor (bFGF) on lead-induced effects. Methods: The primary astroglia cells from 1-6 d old Wistar rats were cultured. The cells pretreated with the MEK1 (mitogen-activated protein kinase kinase 1) inhibitor PD98059 and bFGF, respectively, were exposed to Pb acetate of different concentrations for different times. Western blotting and reverse transcription polymerase chain reaction (RT-PCR) methods were used to detect the protein and mRNA expressions of ERK. Results: mRNA expression for ERK peaked 15 min after initiation of lead exposure (P〈0.05) and protein expression of p-ERK peaked at 30 min (P〈0.05). ERK mRNA levels and p-ERK protein levels returned to baseline after 60 and 120 min of lead exposure, respectively (P〉0.05). The increase in p-ERK levels in lead-treated cells could be inhibited by PD098059. Activation of ERK in the cells by lead was prevented by pretreatment with bFGF. Total ERK protein levels did not change under the same experimental conditions (P〉0.05). Conclusion: Low-level lead exposure resulted in transient activation of ERK through the MEK pathway, which then returned to basal levels in the continued presence of lead. Exogenous bFGF protected ERK signaling components in astroglia from lead poisoning. 展开更多
关键词 LEAD ASTROGLIA Extracellular signal regulated kinase (ERK) Basic fibroblast growth factor (bFGF)
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