The taxonomic relationship of Chinese GeBdium tsengii and Gelidium johnstonii was ambiguous. For almost 20 years they have been regarded as distinct taxa and until 2002 G.johnstonii was considered as a misapplied name...The taxonomic relationship of Chinese GeBdium tsengii and Gelidium johnstonii was ambiguous. For almost 20 years they have been regarded as distinct taxa and until 2002 G.johnstonii was considered as a misapplied name of G. tsengii. In this study, herbarium specimens that initially attributed to G. tsengii and fresh G. tsengii specimens were used to address the taxonomic issues. In phylogenetic studies, G. tsengii from Dayawan, China, near the type locality of G. tsengii and G.johnstonii from Sonora, Mexico, the type locality of G. johnstonii, formed a monophyletic group with maximum support in rbcL and COl genes analyses, indicating that they were genetically identical. In morphological studies, G. tsengii was similar to G. johnstonii in branching pattern, inner structures and fructiferous organs. Consequently, we considered that semi-circular outline of G. tsengii could no longer be treated as a discrimating fea^re. G.johnstonii had priority of publication and according to the International Code of Botanical Nomenclature, G. tsengii was proposed as a synonym of G. johnstonii. Gelidium honghaiwanense sp. nov. was described from Guangdong, China on the basis of morphological and molecular data. For vegetative structures, it was characterized by flattened upright frond, regular two-three times branches pinnate or alternate and clavate ultimate branchlets. For reproductive structures, the tetrasporangial sori were in the apical part of branches and the tetrasporangial branchlets were distichously distributed along second order branches. The present study clarified the relationship between G. tsengii and G. johnstonii from Guangdong and added a new Gelidium species to the Chinese algal flora.展开更多
目的：分析 EDTA 依赖性血小板计数假性偏低（EDP）患者的临床分布并探讨其实验室影响因素及处理方法。方法回顾性分析86例 EDP 患者的临床分布，并对其中20例 EDTA 抗凝管再次采血（方法1）、原分析标本37℃水浴15 min 震荡20 s 后（方...目的：分析 EDTA 依赖性血小板计数假性偏低（EDP）患者的临床分布并探讨其实验室影响因素及处理方法。方法回顾性分析86例 EDP 患者的临床分布，并对其中20例 EDTA 抗凝管再次采血（方法1）、原分析标本37℃水浴15 min 震荡20 s 后（方法2），于室温下即刻、15 min、30 min 时血小板计数并作推片镜检复核，分别与未抗凝血即刻检测结果比较分析相关性及误差可否接受性。结果方法1室温下即刻、15 min 时，与未抗凝血即刻检测结果比较相关性好（r =0.999、0.976），推片镜检血小板未发生聚集；30 min 时相关性较差（r =0.508），推片镜检血小板聚集成团、成簇分布；即刻血小板计数误差可接受，15 min 时临床低水平下误差可接受、高水平下误差不可接受，30 min 时误差不可接受。方法2室温下三个时间点与未抗凝血即刻检测结果相关性均好（r =0.999、0.998、0.996）、推片镜检血小板均未发生聚集，误差均可接受。结论 EDP 患者原分析标本37℃水浴15 min 时震荡20 s 后再次血小板计数可作为报告依据。展开更多
基金Supported by the Strategic Leading Science and Technology Projects of Chinese Academy of Sciences(Nos.XDA11020404,XDA11020304)the China Postdoctoral Science Foundation(No.2016M592260)+1 种基金the National Natural Science Foundation of China(No.41376164)the Scientific and Technological Innovation Project fi nancially supported by Qingdao National Laboratory for Marine Science and Technology(No.2016ASKJ02)
文摘The taxonomic relationship of Chinese GeBdium tsengii and Gelidium johnstonii was ambiguous. For almost 20 years they have been regarded as distinct taxa and until 2002 G.johnstonii was considered as a misapplied name of G. tsengii. In this study, herbarium specimens that initially attributed to G. tsengii and fresh G. tsengii specimens were used to address the taxonomic issues. In phylogenetic studies, G. tsengii from Dayawan, China, near the type locality of G. tsengii and G.johnstonii from Sonora, Mexico, the type locality of G. johnstonii, formed a monophyletic group with maximum support in rbcL and COl genes analyses, indicating that they were genetically identical. In morphological studies, G. tsengii was similar to G. johnstonii in branching pattern, inner structures and fructiferous organs. Consequently, we considered that semi-circular outline of G. tsengii could no longer be treated as a discrimating fea^re. G.johnstonii had priority of publication and according to the International Code of Botanical Nomenclature, G. tsengii was proposed as a synonym of G. johnstonii. Gelidium honghaiwanense sp. nov. was described from Guangdong, China on the basis of morphological and molecular data. For vegetative structures, it was characterized by flattened upright frond, regular two-three times branches pinnate or alternate and clavate ultimate branchlets. For reproductive structures, the tetrasporangial sori were in the apical part of branches and the tetrasporangial branchlets were distichously distributed along second order branches. The present study clarified the relationship between G. tsengii and G. johnstonii from Guangdong and added a new Gelidium species to the Chinese algal flora.
文摘目的：分析 EDTA 依赖性血小板计数假性偏低（EDP）患者的临床分布并探讨其实验室影响因素及处理方法。方法回顾性分析86例 EDP 患者的临床分布，并对其中20例 EDTA 抗凝管再次采血（方法1）、原分析标本37℃水浴15 min 震荡20 s 后（方法2），于室温下即刻、15 min、30 min 时血小板计数并作推片镜检复核，分别与未抗凝血即刻检测结果比较分析相关性及误差可否接受性。结果方法1室温下即刻、15 min 时，与未抗凝血即刻检测结果比较相关性好（r =0.999、0.976），推片镜检血小板未发生聚集；30 min 时相关性较差（r =0.508），推片镜检血小板聚集成团、成簇分布；即刻血小板计数误差可接受，15 min 时临床低水平下误差可接受、高水平下误差不可接受，30 min 时误差不可接受。方法2室温下三个时间点与未抗凝血即刻检测结果相关性均好（r =0.999、0.998、0.996）、推片镜检血小板均未发生聚集，误差均可接受。结论 EDP 患者原分析标本37℃水浴15 min 时震荡20 s 后再次血小板计数可作为报告依据。
