Comparative genetic studies have shown that there are widespread synteny and colinearity of the genes among different species within grass family. Rice ( Oryza sativa L.) is a model plant, and analysis of its genome a...Comparative genetic studies have shown that there are widespread synteny and colinearity of the genes among different species within grass family. Rice ( Oryza sativa L.) is a model plant, and analysis of its genome allows us to reveal the common features and the evolutionary rules of the gramineous genomes and accumulate the data for establishment of a common genetic system in the Poaceae. In this study, a rice gene Pib ( 10.3 kb), a map-based cloned gene, and RFLP markers linked with it are used as the tested probes to investigate their homology and physical location among the tested species. Southern blotting analysis showed that there were sequences homologous to Pib in maize genome. Further, Pib was localized onto the chromosomes of O. sativa ssp. indica cv. Guangluai 4, O. officinalis Wall ex Watt and the inbred line of Zea mays cv. Huangzao 4. The results of fluorescence in situ hybridization (FISH) and double-color FISH indicated that a synteny of Pib and RFLP markers linked with Pib existed among the genomes of the three tested species.展开更多
The goal of this research is to investigate the ideas that functioned in shaping thought of Radif in Iranian Dastgahi music. In this regard by preparing the reasons, we pay attention to the function of education in co...The goal of this research is to investigate the ideas that functioned in shaping thought of Radif in Iranian Dastgahi music. In this regard by preparing the reasons, we pay attention to the function of education in codification of Radif. Although Iranian traditional music has long history but its codification to seven Dastgah-s and five Avaz-es in the format of Radif is attributed to Qajar Dynasty in the 19th century. In this transformation, Mirza Abdollah, Tar-Player of the Nasser al-Din Shah Period, has played an important role in final codification of Radif. Due to the truth that the past masters scrimped in music education, and Mirza Abdollah had encountered with problems to learn music, hence he has mostly cared about music education in adulthood, and it is probable that one of the reasons in collecting Gusheh-s （approximately a small piece of the Iranian Dastgahi music） and organizing them into coherent sets was making simplicity for the students to learn. Furthermore, masters who seriously and continuously have taught after Mirza Abdollah, left a narration （version） of Radif. On the other hand, definite patterns in placement of Gusheh-s and phrases of the Radif have been organized in a manner that makes educational function for it.展开更多
The safeguarding of Venice and the Venetian lagoon （Italy） from rising water is of great national interest. Besides the normal tidal regime, the effects of an increase in the frequency and intensity of floods in the...The safeguarding of Venice and the Venetian lagoon （Italy） from rising water is of great national interest. Besides the normal tidal regime, the effects of an increase in the frequency and intensity of floods in the lagoon have been accentuated by a reduction in the elevation of the land in respect to the actual average sea level. With the aim of reducing the vulnerability of the lagoon, the Italian Ministry of University and Scientific and Technological Research financed the research project ＂The geotechnical model of the subsurface for the safeguarding of Venice and its lagoon＂, to study the compressibility of the lagoon deposits, both by natural causes and as a consequence of overloading with heavy structures. The project involved the construction of a large experimental embankment in the Treporti area of the lagoon, in order to perform a full-scale long-term compression test on the lagoon soils. By using various geomatic and geotechnical monitoring techniques, it was possible to appraise very small vertical movements of the ground induced by the compressibility of the highly heterogeneous silty formations. High-precision data was acquired by means of classical topographic methodologies integrated with continuous GPS （Global Positioning System） measurements. In addition, vertical displacements of the ground were obtained using geotechnical instrumentation. This cross-monitoring approach provided a valuable tool for analyzing both the natural and anthropic causes of settlement.展开更多
To evaluate the Plasmodium falciparum CTL epitope vaccines in HLA class I allele specific human cell lines that have high frequency among Chinese population. Methods. Synthesized oligonucleotides encoding for P.f. CTL...To evaluate the Plasmodium falciparum CTL epitope vaccines in HLA class I allele specific human cell lines that have high frequency among Chinese population. Methods. Synthesized oligonucleotides encoding for P.f. CTL epitope genes, constructed eukaryotic expression plasmids, transfected the minigenes into HLA class I allele specific human cell lines and identified endogenous expressing of the minigenes by RT- PCR and HLA stabilization assay. Results. Two mini- genes encoding Plasmodium falciparum CTL epitopes were designed and cloned, respectively, into an eukaryotic expressing vector to form TR26 which was restricted to HLA- B51, SH6 which was restricted to HLA- A2.1, and TS, which had the two aforementioned mini- genes fused in tandem. All of these CTL epitope genes were transfected and endogenously expressed in respective cell lines containing appropriate HLA molecules. The obviously increased expressions of HLA class I molecules were detected in the transfected cell lines. It was demonstrated that the two discrete Plasmodium falciparum epitope genes were effectively processed and presented, and the close proximity of the two epitope genes in one chain as in mini- gene TS did not interfere with the processing and presenting of each epitope gene in corresponding cell line. Conclusion. A successful expression and presentation of multiple CTL epitope mini- gene in MHC class I allele specific human cell lines were demonstrated by an in vitro assay, which could be corresponding to the vaccination of CTL vaccines in people with different MHC I molecules. This work also suggested the possibility of constructing a multiple CTL epitope plasmodium falciparum DNA vaccine that could cover most of Chinese population.展开更多
文摘Comparative genetic studies have shown that there are widespread synteny and colinearity of the genes among different species within grass family. Rice ( Oryza sativa L.) is a model plant, and analysis of its genome allows us to reveal the common features and the evolutionary rules of the gramineous genomes and accumulate the data for establishment of a common genetic system in the Poaceae. In this study, a rice gene Pib ( 10.3 kb), a map-based cloned gene, and RFLP markers linked with it are used as the tested probes to investigate their homology and physical location among the tested species. Southern blotting analysis showed that there were sequences homologous to Pib in maize genome. Further, Pib was localized onto the chromosomes of O. sativa ssp. indica cv. Guangluai 4, O. officinalis Wall ex Watt and the inbred line of Zea mays cv. Huangzao 4. The results of fluorescence in situ hybridization (FISH) and double-color FISH indicated that a synteny of Pib and RFLP markers linked with Pib existed among the genomes of the three tested species.
文摘The goal of this research is to investigate the ideas that functioned in shaping thought of Radif in Iranian Dastgahi music. In this regard by preparing the reasons, we pay attention to the function of education in codification of Radif. Although Iranian traditional music has long history but its codification to seven Dastgah-s and five Avaz-es in the format of Radif is attributed to Qajar Dynasty in the 19th century. In this transformation, Mirza Abdollah, Tar-Player of the Nasser al-Din Shah Period, has played an important role in final codification of Radif. Due to the truth that the past masters scrimped in music education, and Mirza Abdollah had encountered with problems to learn music, hence he has mostly cared about music education in adulthood, and it is probable that one of the reasons in collecting Gusheh-s （approximately a small piece of the Iranian Dastgahi music） and organizing them into coherent sets was making simplicity for the students to learn. Furthermore, masters who seriously and continuously have taught after Mirza Abdollah, left a narration （version） of Radif. On the other hand, definite patterns in placement of Gusheh-s and phrases of the Radif have been organized in a manner that makes educational function for it.
文摘The safeguarding of Venice and the Venetian lagoon （Italy） from rising water is of great national interest. Besides the normal tidal regime, the effects of an increase in the frequency and intensity of floods in the lagoon have been accentuated by a reduction in the elevation of the land in respect to the actual average sea level. With the aim of reducing the vulnerability of the lagoon, the Italian Ministry of University and Scientific and Technological Research financed the research project ＂The geotechnical model of the subsurface for the safeguarding of Venice and its lagoon＂, to study the compressibility of the lagoon deposits, both by natural causes and as a consequence of overloading with heavy structures. The project involved the construction of a large experimental embankment in the Treporti area of the lagoon, in order to perform a full-scale long-term compression test on the lagoon soils. By using various geomatic and geotechnical monitoring techniques, it was possible to appraise very small vertical movements of the ground induced by the compressibility of the highly heterogeneous silty formations. High-precision data was acquired by means of classical topographic methodologies integrated with continuous GPS （Global Positioning System） measurements. In addition, vertical displacements of the ground were obtained using geotechnical instrumentation. This cross-monitoring approach provided a valuable tool for analyzing both the natural and anthropic causes of settlement.
基金This project was supported by the National Natural Sciences Foun-dation of China, grant# 39770670 and China Medical Bo
文摘To evaluate the Plasmodium falciparum CTL epitope vaccines in HLA class I allele specific human cell lines that have high frequency among Chinese population. Methods. Synthesized oligonucleotides encoding for P.f. CTL epitope genes, constructed eukaryotic expression plasmids, transfected the minigenes into HLA class I allele specific human cell lines and identified endogenous expressing of the minigenes by RT- PCR and HLA stabilization assay. Results. Two mini- genes encoding Plasmodium falciparum CTL epitopes were designed and cloned, respectively, into an eukaryotic expressing vector to form TR26 which was restricted to HLA- B51, SH6 which was restricted to HLA- A2.1, and TS, which had the two aforementioned mini- genes fused in tandem. All of these CTL epitope genes were transfected and endogenously expressed in respective cell lines containing appropriate HLA molecules. The obviously increased expressions of HLA class I molecules were detected in the transfected cell lines. It was demonstrated that the two discrete Plasmodium falciparum epitope genes were effectively processed and presented, and the close proximity of the two epitope genes in one chain as in mini- gene TS did not interfere with the processing and presenting of each epitope gene in corresponding cell line. Conclusion. A successful expression and presentation of multiple CTL epitope mini- gene in MHC class I allele specific human cell lines were demonstrated by an in vitro assay, which could be corresponding to the vaccination of CTL vaccines in people with different MHC I molecules. This work also suggested the possibility of constructing a multiple CTL epitope plasmodium falciparum DNA vaccine that could cover most of Chinese population.
