Verticillium dahliae is a soil-borne phytopathogenic fungus that causes vascular wilt disease in a broad range of hosts. This pathogen survives for many years in soil in the form ofmelanized microsclerotia. To investi...Verticillium dahliae is a soil-borne phytopathogenic fungus that causes vascular wilt disease in a broad range of hosts. This pathogen survives for many years in soil in the form ofmelanized microsclerotia. To investigate the melanin synthesis in V.. dahliae, we identified a polyketide synthase gene in V. dahliae, namely VdPKS1. PKS1 is known to involve in the dihydroxynaphthalene melanin synthesis pathway in many fungi. We found that VdPKS1 was required for melanin formation but not for microsclerotial production in E dahliae. The VdPKS1 gene-disruption mutant （vdpksl） formed melanin-deficient albino microsclerotia, which did not affect the fungal colonization in host tissues but significantly reduced the disease severity. Gene transcription analysis in the wild-type and the vdpks1 strains suggested that VdPKS1 gene-disruption influenced the expression of a series of genes involved in ethylene biosynthesis, microsclerotial formation and pathogenesis. Our results suggest that the VdPKS1-mediated melanin synthesis is important for virulence and developmental traits of E dahliae.展开更多
In paddy soils of Thailand, the addition of organic matter （OM） is used to efficiently limit the effect of salinity on rice culture and production. OM used as an amendment and fertilizer promotes the reduced conditi...In paddy soils of Thailand, the addition of organic matter （OM） is used to efficiently limit the effect of salinity on rice culture and production. OM used as an amendment and fertilizer promotes the reduced condition and increases iron solubilization without provoking ferrous toxicity. In this study, the intricate biogeochemical role of iron-reducing bacteria （IRB） involved in the quality of water and soil of paddy fields, particularly when the paddy fields were subject to salinity and organic matter addition, were studied in paddy fields of Thailand. The results demonstrated that the addition of OM increased the proliferation of cultivable IRB and their specific activity. Cultivable IRB communities decreased in the presence of salt. The presence of salt modified the structure of the bacterial populations by favoring the development of alkaline and moderately halophilic bacteria （Virgibacillus spp., Occanobacillus spp., and PaenibaciUus spp.）. The paddy soils studied contained very diversified （halosensitive, halotolerant, and halophilic） IRB populations that could be adapted to a variety of salinity conditions （0-90 g L-1 NaCI） using different organic substrates （glucose, acetate, and soil organic matter） to maintain significant activities under extreme conditions of salinity. The rhizosphere of rice stimulated IRB community growth without organic matter, whereas organic matter addition limited the rhizosphere effect on IRB cultivable number in saline condition. The interactive action of salinity and organic amendment had a negative impact on the rhizosphere effect. The presence of specific iron-reducing populations （fermentative, iron-respiring, anaerobic, and facultative anaerobic）, having different behaviors under salt and redox stresses, appeared to be a key factor that contributed to the control or enhancement of the quality of water and soil in paddy fields.展开更多
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB11040500)the National Natural Science Foundation of China(31160351,311060350)Basic Research Projects of Xingjiang Production and Construction Corps(2016AG001)
文摘Verticillium dahliae is a soil-borne phytopathogenic fungus that causes vascular wilt disease in a broad range of hosts. This pathogen survives for many years in soil in the form ofmelanized microsclerotia. To investigate the melanin synthesis in V.. dahliae, we identified a polyketide synthase gene in V. dahliae, namely VdPKS1. PKS1 is known to involve in the dihydroxynaphthalene melanin synthesis pathway in many fungi. We found that VdPKS1 was required for melanin formation but not for microsclerotial production in E dahliae. The VdPKS1 gene-disruption mutant （vdpksl） formed melanin-deficient albino microsclerotia, which did not affect the fungal colonization in host tissues but significantly reduced the disease severity. Gene transcription analysis in the wild-type and the vdpks1 strains suggested that VdPKS1 gene-disruption influenced the expression of a series of genes involved in ethylene biosynthesis, microsclerotial formation and pathogenesis. Our results suggest that the VdPKS1-mediated melanin synthesis is important for virulence and developmental traits of E dahliae.
基金Supported by the ECCO Program of the National Institute for Earth Sciences and Astronomy (INSU)French National Center for Scientific Research (CNRS)+1 种基金the Collaboration between the Institute for Research and Development (IRD)the Land Department and Development (LDD) of Thailand International Cooperation Agency (TICA)
文摘In paddy soils of Thailand, the addition of organic matter （OM） is used to efficiently limit the effect of salinity on rice culture and production. OM used as an amendment and fertilizer promotes the reduced condition and increases iron solubilization without provoking ferrous toxicity. In this study, the intricate biogeochemical role of iron-reducing bacteria （IRB） involved in the quality of water and soil of paddy fields, particularly when the paddy fields were subject to salinity and organic matter addition, were studied in paddy fields of Thailand. The results demonstrated that the addition of OM increased the proliferation of cultivable IRB and their specific activity. Cultivable IRB communities decreased in the presence of salt. The presence of salt modified the structure of the bacterial populations by favoring the development of alkaline and moderately halophilic bacteria （Virgibacillus spp., Occanobacillus spp., and PaenibaciUus spp.）. The paddy soils studied contained very diversified （halosensitive, halotolerant, and halophilic） IRB populations that could be adapted to a variety of salinity conditions （0-90 g L-1 NaCI） using different organic substrates （glucose, acetate, and soil organic matter） to maintain significant activities under extreme conditions of salinity. The rhizosphere of rice stimulated IRB community growth without organic matter, whereas organic matter addition limited the rhizosphere effect on IRB cultivable number in saline condition. The interactive action of salinity and organic amendment had a negative impact on the rhizosphere effect. The presence of specific iron-reducing populations （fermentative, iron-respiring, anaerobic, and facultative anaerobic）, having different behaviors under salt and redox stresses, appeared to be a key factor that contributed to the control or enhancement of the quality of water and soil in paddy fields.
