目的:细胞基质蛋白FRP(Follistatin related protein,FRP)具有调节细胞增殖与迁移的功能,探讨诱导体外培养血管平滑肌细胞表达FRP的机制研究。方法:分离培养脐动脉和人乳内动脉血管平滑肌细胞(vascularsmooth muscle cell,VSMC);确定植...目的:细胞基质蛋白FRP(Follistatin related protein,FRP)具有调节细胞增殖与迁移的功能,探讨诱导体外培养血管平滑肌细胞表达FRP的机制研究。方法:分离培养脐动脉和人乳内动脉血管平滑肌细胞(vascularsmooth muscle cell,VSMC);确定植物雌激素类药物大豆苷元抑制VSMC增殖的最佳作用时间与浓度;鉴定雌激素受体α、β;给予大豆苷元、17-β雌二醇和雌激素受体完全阻断剂ICI182780刺激细胞,进行竞争性反转录PCR、Northern Blot和原位杂交探讨药物诱导FRP表达途径。结果:大豆苷元可抑制两种VSMC的增殖;脐动脉VSMC表达雌激素受体α,人乳内动脉VSMC表达受体α、β;大豆苷元和雌激素可显著诱导FRP在两种不同VSMC中的表达,ICI182780可抑制大豆苷元的诱导作用。结论:细胞基质蛋白FRP可被雌激素类药物通过其受体诱导。展开更多
Planting date is a critical component of soybean [Glycine max （L.） Merr.] production, under dry land conditions in the Southeastern Coastal Plain. The objectives of this study were to 1. Evaluate the effect of plant...Planting date is a critical component of soybean [Glycine max （L.） Merr.] production, under dry land conditions in the Southeastern Coastal Plain. The objectives of this study were to 1. Evaluate the effect of planting date on plant leaf area index （LAI） and normalized difference vegetation index （NDVI） at 60 and 90 days after planting （DAP）, plant height and grain yield, and 2. Determine the optimum planting period by integrating the responses from vegetation growth to yield for soybean maturity group （MG） IV-VIII under dry land conditions in the Southeastern Coastal Plain. Planting dates were scheduled about 14-days intervals from late April to mid-July （2008） or late July （2009）. Greatest grain yield for MG IV was obtained from planting in around mid-May in both years. The yield was greater for MG V planted in May and greater for MG VI-VIII planted in late April and May, but started to decline for planting in early June. Plant LAI and NDVI at 60 DAP were affected by both planting date and precipitation, but were poorly correlated with grain yield. However, plant LAI and NDVI were well correlated with yield and were greater for May planting dates at 90 DAP. These indiccs declined for soybean planted after May. Mature plant height decreased more rapidly with delayed planting. These results indicate that plant growth and yield decreased after May planting. Optimum planting period for all MGs was early to mid-May.展开更多
文摘Planting date is a critical component of soybean [Glycine max （L.） Merr.] production, under dry land conditions in the Southeastern Coastal Plain. The objectives of this study were to 1. Evaluate the effect of planting date on plant leaf area index （LAI） and normalized difference vegetation index （NDVI） at 60 and 90 days after planting （DAP）, plant height and grain yield, and 2. Determine the optimum planting period by integrating the responses from vegetation growth to yield for soybean maturity group （MG） IV-VIII under dry land conditions in the Southeastern Coastal Plain. Planting dates were scheduled about 14-days intervals from late April to mid-July （2008） or late July （2009）. Greatest grain yield for MG IV was obtained from planting in around mid-May in both years. The yield was greater for MG V planted in May and greater for MG VI-VIII planted in late April and May, but started to decline for planting in early June. Plant LAI and NDVI at 60 DAP were affected by both planting date and precipitation, but were poorly correlated with grain yield. However, plant LAI and NDVI were well correlated with yield and were greater for May planting dates at 90 DAP. These indiccs declined for soybean planted after May. Mature plant height decreased more rapidly with delayed planting. These results indicate that plant growth and yield decreased after May planting. Optimum planting period for all MGs was early to mid-May.
