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中华蜜蜂越冬期抗寒生理生化指标研究 被引量:3
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作者 苏晓玲 陈道印 +2 位作者 赵东绪 华启云 罗谷辉 《环境昆虫学报》 CSCD 北大核心 2022年第3期586-594,共9页
本文研究活框饲养和原始饲养中华蜜蜂Apis cerana cerana Fabricius越冬期的抗寒生理生化指标变化,为中蜂抗寒生理机制和科学饲养管理提供理论依据。结果表明,两种饲养方式蜜蜂的体重、蛋白质和甘油含量均由越冬初期开始上升,越冬中后... 本文研究活框饲养和原始饲养中华蜜蜂Apis cerana cerana Fabricius越冬期的抗寒生理生化指标变化,为中蜂抗寒生理机制和科学饲养管理提供理论依据。结果表明,两种饲养方式蜜蜂的体重、蛋白质和甘油含量均由越冬初期开始上升,越冬中后期达到最高而后下降;游离水和糖原含量均在越冬中后期出现下降;除原始饲养中蜂的CAT酶外,两种饲养方式中蜂的越冬期超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)酶活均在越冬中期降到最低,而总抗氧化能力呈逐渐升高趋势。但活框饲养中蜂越冬期游离水(P<0.05)和糖原含量(P>0.05)均低于原始饲养中蜂,甘油含量显著高于原始饲养中蜂(P<0.05);活框饲养中蜂与原始饲养中蜂的3种抗氧化酶活性在不同时期存在显著差异,活框饲养中蜂总抗氧化能力显著高于原始饲养中蜂(P<0.05)。蜜蜂在自然越冬时通过降低体内游离水含量,增加甘油含量来增加抗寒能力,并贮备大量糖原和蛋白质供越冬期能量消耗;同时,其体内的抗氧化酶在越冬期间发挥协同作用,减少低温刺激造成的氧化应激反应。 展开更多
关键词 中华蜜蜂 越冬 生理特性 抗氧化 活框饲养 原始饲养
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Screening of Deltamethrin Resistant Strain of Bombyx Mandarina 被引量:3
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作者 赵华强 刘海涛 +3 位作者 王东 李兵 许雅香 沈卫德 《Agricultural Science & Technology》 CAS 2009年第2期138-141,共4页
[ Objective ] The aim of the study was to explore the resistance development of deltamethrin through selection of dettamethrin resistant strain of wild silkworm ( Bombyx Mandarina), thus providing the basis for scie... [ Objective ] The aim of the study was to explore the resistance development of deltamethrin through selection of dettamethrin resistant strain of wild silkworm ( Bombyx Mandarina), thus providing the basis for scientific pesticide application and resistance management. [ Method] The wild silkworms collected from three different regions were reared indoors, and the sensitivity of their parents to deltamethrin was detected by topical application. The larvae in each generation were treated with deltamethdn in median lethal dose or so by.topical application. The mortality of larva was analyzed for the establishment of toxicity regression equations and the calculation of the multiple or increased multiple of deltamethdn resistance.[Result] After the Qidong Bornbyx Mandarina (YQD) fed with mulberry leaves were selected for three generations indoors, the multiple of deltamethdn resistance of F, was 14.26, 1.2 times as great as that of Fo ; after the Bombyx Mandarina from mulberry garden of Soochow University (YSD) fed with mulberry leaves were selected for three generations indoors, the multiple of deltamethrin resistance of F4 was 16.48, 1.9 times as great as that of Fo ; after the Wujiang Bombyx Mandarina (YWJ) fed with artificial diets were selected for three generations indoors during six generations, the multiple of deltamethrin resistance of Fe was 18.67, 1.2 times as great as that of Fo. [ Conclusioa] With the selection in same dose, the resistance multiple of YSD increases more rapidly than that of YQD; under double selection of artificial diets and insecticide, the resistance multiple of YWJ increases more slowly than that of YQD. 展开更多
关键词 Bombyx Mandarina DELTAMETHRIN RESISTANCE
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