Beta Ti−35Nb sandwich-structured composites with various reinforcing layers were designed and produced using additive manufacturing(AM)to achieve a balance between light weight and high strength.The impact of reinforc...Beta Ti−35Nb sandwich-structured composites with various reinforcing layers were designed and produced using additive manufacturing(AM)to achieve a balance between light weight and high strength.The impact of reinforcing layers on the compressive deformation behavior of porous composites was investigated through micro-computed tomography(Micro-CT)and finite element method(FEM)analyses.The results indicate that the addition of reinforcement layers to sandwich structures can significantly enhance the compressive yield strength and energy absorption capacity of porous metal structures;Micro-CT in-situ observation shows that the strain of the porous structure without the reinforcing layer is concentrated in the middle region,while the strain of the porous structure with the reinforcing layer is uniformly distributed;FEM analysis reveals that the reinforcing layers can alter stress distribution and reduce stress concentration,thereby promoting uniform deformation of the porous structure.The addition of reinforcing layer increases the compressive yield strength of sandwich-structured composite materials by 124%under the condition of limited reduction of porosity,and the yield strength increases from 4.6 to 10.3 MPa.展开更多
The microbiotic crust study is among new focuses in investigating on the desertification control. Based on determination of algal crusts with different successive ages (4-, 8-, 17-, 34-, 42-year-old) and unconsolidate...The microbiotic crust study is among new focuses in investigating on the desertification control. Based on determination of algal crusts with different successive ages (4-, 8-, 17-, 34-, 42-year-old) and unconsolidated sand in the desert area, species composition and clustering analyses were carried out in this study. Results on successional orientation revealed that (1) the abundance of Cyanophyta, specially of Scytonema javanicum gradually decreased; (2) the abundance of Chlorophyta, Bacillariophyta and a species of Cyanophyta, Phormidium tenue increased; (3) the biodiversity increased gradually with the community succession; and (4) biomass of microalgae increased at the early stage, but decreased at the later stage due to the abundance of lichens and mosses. But, the speed of natural succession was so slow that the community-building species was still the first dominant species after 42 years, except that its dominant degree decreased just slightly. However, successive speed and trend were affected by water, vegetation coverage, terrain, time and soil physico-chemical properties as well, especially Mn content in the soil appeared to have a threshold effect.展开更多
The microstructure of the 18R-type long period stacking ordered (LPSO) phase in Mg 97 Y 2 Zn 1 alloy was investigated by the first principles calculation. The arrangement rule of Zn and Y atoms in the LPSO structure...The microstructure of the 18R-type long period stacking ordered (LPSO) phase in Mg 97 Y 2 Zn 1 alloy was investigated by the first principles calculation. The arrangement rule of Zn and Y atoms in the LPSO structure is determined theoretically. The calculation results reveal that the additive atoms are firstly located in the fault layers at the two ends of the 18R-type LPSO structure, and then extend to fault layers in the interior, which is in good agreement with the experimental observations. This feature also implies the microstructural relationship between 18R and other LPSO structures. The cohesive energy and the formation heat indicate the dependence of the stability of 18R LPSO structure on contents of Y and Zn atoms. The calculated electronic structures reveal the underlying mechanism of microstructure and the stability of 18R LPSO structure.展开更多
First-principles calculations were carried out to investigate the structural stabilities and electronic properties of RhZr.The plane wave based pseudopotential method was used,in which both the local density approxima...First-principles calculations were carried out to investigate the structural stabilities and electronic properties of RhZr.The plane wave based pseudopotential method was used,in which both the local density approximation(LDA) and the generalized gradient approximation(GGA) implanted in the CASTEP code were employed.The internal positions of atoms in the unit cell were optimized and the ground state properties such as lattice parameter,density of state,cohesive energies and enthalpies of formation of ortho-RhZr and cubic-RhZr were calculated.The calculation results indicate that ortho-RhZr can form more easily than cubic-RhZr and the ortho-RhZr is more stable than cubic-RhZr.The density of states(DOS) reveals that the strong bonding in the Rh-Zr and Rh-Rh or Zr-Zr interaction chains accounts for the structural stability of ortho-RhZr and the hybridization between Rh-4d states and Zr-4d states is strong.展开更多
The dynamic changes of land system in Huang - Huai - Hai Plain between 1988 and 2000 were researched in this paper. Spatial dominance econometric model was estabilished on 1 km cell to quantificafionally analyze the d...The dynamic changes of land system in Huang - Huai - Hai Plain between 1988 and 2000 were researched in this paper. Spatial dominance econometric model was estabilished on 1 km cell to quantificafionally analyze the driving-force for the dynamic change mechanism of land system, such as natural, social and economic factors. The future dynamic changes of land system in Huang - Huai - Hai Plain on each 1 km cell during 2000 to 2020 were stimulated by combining the dynamic changes of land system on each 1 km cell with different situations. The research indicated that the dynamic changes of land system structure changed mainly from the cultivated areas to building areas and industrial areas, and forest areas increased during this period. Although the revolutions of land system structure were different during 2000 to 2020 with the different referrence standard, ecological protection and economic development, the primary dynamic changes of land system structure were that the increase of building land areas with the decline cuhivaled land areas and the increase of woodlands.展开更多
The potential energies, volumes and electronic structures of characteristic atoms coordinated by neighboring configurations were obtained from the experimental heats of formation and lattice parameters of disordered A...The potential energies, volumes and electronic structures of characteristic atoms coordinated by neighboring configurations were obtained from the experimental heats of formation and lattice parameters of disordered Au1-xCux alloys. From characteristic atom occupation (CAO) patterns of L12-Au3Cu, L12-AuCu3 and Llo-AuCu compounds, their electronic structures, volumetric and energetic properties were calculated. The CAO pattern of Johasson-Linde(J-L) model shows that the transition AuCuI→AuCulI is an exothermic and volume contraction reaction, which is opposite from experimental phenomena. According to CAO pattern of Guymont-Feutelais-Legendre(G-F-L) model, the AuCulI cell consists of two periodic antidirection (PAD) AuCuI regions and two PAD boundary regions. The equations derived from CAO pattern of G-F-L model can be used to calculate energetic properties, volumetric properties and ordering degrees of the PAD AuCuI region and PAD boundary region, as well as corresponding average properties of the AuCulI phase. The results are consistent with experimental phenomena.展开更多
The electronic structures,chemical bonding,elastic and optical properties of the ternary stannide phase Na2MgSn were investigated by using density-fimctional theory(DFT) within generalized gradient approximation(GG...The electronic structures,chemical bonding,elastic and optical properties of the ternary stannide phase Na2MgSn were investigated by using density-fimctional theory(DFT) within generalized gradient approximation(GGA).The calculated energy band structures show that Na2MgSn is an indirect semiconductor material with a narrow band gap 0.126 eV.The density of state(DOS)and the partial density of state(PDOS) calculations show that the DOS near the Fermi level is mainly from the Na 2p,Mg 3p and Sn5 p states.Population analysis suggests that there are strongly bonded Mg-Sn honeycomb layers in Na2MgSn.Basic physical properties,such as lattice constant,bulk modulus,shear modulus,elastic constants c(ij) were calculated.The elastic modulus E and Poisson ratio v were also predicted.The results show that Na2MgSn is mechanically stable soft material and behaves in a brittle manner.Detailed analysis of all optical functions reveals that Na2MgSn is a better dielectric material,and reflectivity spectra show that Na2MgSn promise as good coating materials in the energy regions 6.24-10.49 eV.展开更多
The microstructure of asphalt is investigated by atomic force microscopy (AFM). In order to analyze the impacts of asphalt types on microstructures, two neat asphalts with different penetration grades (50# and 70#...The microstructure of asphalt is investigated by atomic force microscopy (AFM). In order to analyze the impacts of asphalt types on microstructures, two neat asphalts with different penetration grades (50# and 70#) and one styrene-butadiene-styrene (SBS) modified asphalt are chosen. The influence of short-term aging is also studied. Based on the knowledge of asphalt's microproperties, the relationship between microstructures and healing property is analyzed. The results indicate that the microstructures of three asphalts are quite different and the effects of aging on the surface characteristics for different asphalts are also different. It is proposed that the bee structure is a type of wax crystal and it has a close relationship with the "bridge-healing" mechanism. The findings may reveal the formation mechanism of microstructure and the healing property for asphalts.展开更多
The electric pulse modification (EP, EPM) of liquid metal is a novel method for grain refinement. The structure of EP-modified Al-5%Cu melt was characterized by high-temperature X-ray diffractometry. The results sho...The electric pulse modification (EP, EPM) of liquid metal is a novel method for grain refinement. The structure of EP-modified Al-5%Cu melt was characterized by high-temperature X-ray diffractometry. The results show that the Cu-containing Al clusters remarkably increase in the EP-modified melt, furthermore, these clusters in that case tend to contract due to the decrease of relevant atomic radius and the co-ordination number. This kind of liquid-phase structure leads to a more homogeneous Cu-rich phase distribution in the final solidification structure. Differential scanning calorimetry (DSC) tests indicate that the solidification super-cooling degree of the EP-modified liquid phase is 2.36 times that of the unmodified. These facts suggest that the atom cluster changes in EP-modified Al-5%Cu melt would disagree with that by EPM model previously proposed in liquid pure metal.展开更多
The structural stability, elastic and electronic properties under pressure at 0 K for β-Ti have been investigated by per-forming first-principles calculations. With the increase of pressure, the structure of β-Ti b...The structural stability, elastic and electronic properties under pressure at 0 K for β-Ti have been investigated by per-forming first-principles calculations. With the increase of pressure, the structure of β-Ti becomes stabler, which is further con-firmed by the calculation for density of state (DOS). The phase transition pressure of is about 64. 3 GPa, which is consist-ent with other theoretical predictions (63. 7 GPa) and the experimental result (50 GPa). The pressure dependence of elastic constants shows that the low-pressure limit for a mechanically stable β-Ti is about 50 GPa with low Young?s modulus value of about 30. 01 GPa, which approaches the value of a human bone (30 GPa). In addition, the pressure dependence of bulk modu-lus B, shear modulus G, Young’s modulus E,Poisson’s ratio σ,aggregate sound velocities,and ductility/brittleness under different pressures were also discussed. B, G and E ascend monotonously with increasing pressure, while a descends. β-Ti re-mains ductile by analysis of B/G under considered pressures.展开更多
[Objective] The research aimed to provide reference for increasing the genetic transformation efficiency of Ginkgo biloba mediated by Agrobacterium.[Method] Taking the mature embryos of Ginkgo biloba seeds as explants...[Objective] The research aimed to provide reference for increasing the genetic transformation efficiency of Ginkgo biloba mediated by Agrobacterium.[Method] Taking the mature embryos of Ginkgo biloba seeds as explants,after 48 hours' pre-cultivation on MS medium in the absence of phytohormone,GUS gene was transmitted into embryos of Ginkgo biloba mediated by three kinds of Agrobacterium.Transient expression of GUS gene activity was observed through histochemical staining,and the influencing factors of the expression of GUS gene were analyzed.And the expression vector of 1-deoxy-D-xylulose-5-phosphate reductoisomerase in the biosynthesis approach of biobalide precursor of Ginkgo biloba was constructed.[Result] A more suitable genetic transformation scheme was obtained as follows:taking embryos of Ginkgo biloba as explants,using EHA105 Agrobacterium with pCAMBIA1304+ for infection,co-culture for 3 days and GUS staining.The results showed that transient expression rate of GUS after transformation was higher.[Conclusion] The research provide a more effective method for further study on the transgene of Ginkgo biloba.展开更多
The structural stability, electronic structures, elastic properties and thermodynamic properties of the main binary phases Mg_(17)Al_(12), Al_2Ca, Mg_2 Sn and Mg_2 Ca in Mg-Al-Ca-Sn alloy were determined from the ...The structural stability, electronic structures, elastic properties and thermodynamic properties of the main binary phases Mg_(17)Al_(12), Al_2Ca, Mg_2 Sn and Mg_2 Ca in Mg-Al-Ca-Sn alloy were determined from the first-principles calculation. The calculated lattice parameters are in good agreement with the experimental and literature values. The calculated heats of formation and cohesive energies show that Al_2Ca has the strongest alloying ability and structural stability. The densities of states(DOS), Mulliken electron occupation number, metallicity and charge density difference of these compounds are given. The elastic constants of Mg_(17)Al_(12), Al_2Ca, Mg_2 Sn and Mg_2 Ca phases are calculated, and the bulk moduli, shear moduli, elastic moduli and Poisson ratio are derived. The calculations of thermodynamic properties show that the Gibbs free energies of Al_2Ca and Mg_2 Sn are lower than that of Mg_(17)Al_(12), which indicates that Al_2Ca and Mg_2 Sn are more stable than Mg_(17)Al_(12) phase. Hence, the heat resistance of Mg-Al-based alloys can be improved by adding Ca and Sn additions.展开更多
The electronic structures and mechanical properties of Al4Sr, Mg2Sr and Mg23Sr6 phases were determined by the use of first-principles calculations. The calculated heat of formation and cohesive energy indicate that Al...The electronic structures and mechanical properties of Al4Sr, Mg2Sr and Mg23Sr6 phases were determined by the use of first-principles calculations. The calculated heat of formation and cohesive energy indicate that Al4Sr has the strongest alloying ability as well as the highest structural stability. The elastic parameters were calculated, and then the bulk modulus, shear modulus, elastic modulus and Poisson ratio were derived. The ductility and plasticity were discussed. The results show that Al4Sr and Mg2Sr phases both are ductile, on the contrary, Mg23Sr6 is brittle, and among the three phases, Mg2Sr is a phase with the best plasticity.展开更多
基金the Hunan Young Scientific Innovative Talents Program,China(No.2020RC3040)Outstanding Youth Fund of Hunan Natural Science Foundation,China(Nos.2021JJ20011,2021JJ40600,2021JJ40590)the National Natural Science Foundation of China(Nos.52001030,52204371)..
文摘Beta Ti−35Nb sandwich-structured composites with various reinforcing layers were designed and produced using additive manufacturing(AM)to achieve a balance between light weight and high strength.The impact of reinforcing layers on the compressive deformation behavior of porous composites was investigated through micro-computed tomography(Micro-CT)and finite element method(FEM)analyses.The results indicate that the addition of reinforcement layers to sandwich structures can significantly enhance the compressive yield strength and energy absorption capacity of porous metal structures;Micro-CT in-situ observation shows that the strain of the porous structure without the reinforcing layer is concentrated in the middle region,while the strain of the porous structure with the reinforcing layer is uniformly distributed;FEM analysis reveals that the reinforcing layers can alter stress distribution and reduce stress concentration,thereby promoting uniform deformation of the porous structure.The addition of reinforcing layer increases the compressive yield strength of sandwich-structured composite materials by 124%under the condition of limited reduction of porosity,and the yield strength increases from 4.6 to 10.3 MPa.
文摘The microbiotic crust study is among new focuses in investigating on the desertification control. Based on determination of algal crusts with different successive ages (4-, 8-, 17-, 34-, 42-year-old) and unconsolidated sand in the desert area, species composition and clustering analyses were carried out in this study. Results on successional orientation revealed that (1) the abundance of Cyanophyta, specially of Scytonema javanicum gradually decreased; (2) the abundance of Chlorophyta, Bacillariophyta and a species of Cyanophyta, Phormidium tenue increased; (3) the biodiversity increased gradually with the community succession; and (4) biomass of microalgae increased at the early stage, but decreased at the later stage due to the abundance of lichens and mosses. But, the speed of natural succession was so slow that the community-building species was still the first dominant species after 42 years, except that its dominant degree decreased just slightly. However, successive speed and trend were affected by water, vegetation coverage, terrain, time and soil physico-chemical properties as well, especially Mn content in the soil appeared to have a threshold effect.
基金Projects(50861002,51071053)supported by the National Natural Science Foundation of ChinaProject(0991051)supported by NaturalScience Foundation of Guangxi Province,China+1 种基金Project(KF0803)supported by Open Project of Key Laboratory of Materials Design and Preparation Technology of Hunan Province,ChinaProject(X071117)supported by Scientific Research Foundation of Guangxi University,China
文摘The microstructure of the 18R-type long period stacking ordered (LPSO) phase in Mg 97 Y 2 Zn 1 alloy was investigated by the first principles calculation. The arrangement rule of Zn and Y atoms in the LPSO structure is determined theoretically. The calculation results reveal that the additive atoms are firstly located in the fault layers at the two ends of the 18R-type LPSO structure, and then extend to fault layers in the interior, which is in good agreement with the experimental observations. This feature also implies the microstructural relationship between 18R and other LPSO structures. The cohesive energy and the formation heat indicate the dependence of the stability of 18R LPSO structure on contents of Y and Zn atoms. The calculated electronic structures reveal the underlying mechanism of microstructure and the stability of 18R LPSO structure.
基金Project(u0837601)supported by the National Natural Science Foundation of China
文摘First-principles calculations were carried out to investigate the structural stabilities and electronic properties of RhZr.The plane wave based pseudopotential method was used,in which both the local density approximation(LDA) and the generalized gradient approximation(GGA) implanted in the CASTEP code were employed.The internal positions of atoms in the unit cell were optimized and the ground state properties such as lattice parameter,density of state,cohesive energies and enthalpies of formation of ortho-RhZr and cubic-RhZr were calculated.The calculation results indicate that ortho-RhZr can form more easily than cubic-RhZr and the ortho-RhZr is more stable than cubic-RhZr.The density of states(DOS) reveals that the strong bonding in the Rh-Zr and Rh-Rh or Zr-Zr interaction chains accounts for the structural stability of ortho-RhZr and the hybridization between Rh-4d states and Zr-4d states is strong.
基金Supported by the National Natural Science Foundation of China(70503025)the Knowledge Innovation Project of Chinese Academy of Sci-ences(KZCX2-YW-305-2+3 种基金KSCX2-YW-N-039)the National Project of Scientific and Technical Supporting Programs(2006BAC08B032006BAC08B06)international cooperation program of science and technolo-gy ministry(2006DFB919201).~~
文摘The dynamic changes of land system in Huang - Huai - Hai Plain between 1988 and 2000 were researched in this paper. Spatial dominance econometric model was estabilished on 1 km cell to quantificafionally analyze the driving-force for the dynamic change mechanism of land system, such as natural, social and economic factors. The future dynamic changes of land system in Huang - Huai - Hai Plain on each 1 km cell during 2000 to 2020 were stimulated by combining the dynamic changes of land system on each 1 km cell with different situations. The research indicated that the dynamic changes of land system structure changed mainly from the cultivated areas to building areas and industrial areas, and forest areas increased during this period. Although the revolutions of land system structure were different during 2000 to 2020 with the different referrence standard, ecological protection and economic development, the primary dynamic changes of land system structure were that the increase of building land areas with the decline cuhivaled land areas and the increase of woodlands.
基金Project (50711181) supported by the National Natural Science Foundation of China Project (2009FJ4016) supported by Natural Science Foundation of Hunan Province,China
文摘The potential energies, volumes and electronic structures of characteristic atoms coordinated by neighboring configurations were obtained from the experimental heats of formation and lattice parameters of disordered Au1-xCux alloys. From characteristic atom occupation (CAO) patterns of L12-Au3Cu, L12-AuCu3 and Llo-AuCu compounds, their electronic structures, volumetric and energetic properties were calculated. The CAO pattern of Johasson-Linde(J-L) model shows that the transition AuCuI→AuCulI is an exothermic and volume contraction reaction, which is opposite from experimental phenomena. According to CAO pattern of Guymont-Feutelais-Legendre(G-F-L) model, the AuCulI cell consists of two periodic antidirection (PAD) AuCuI regions and two PAD boundary regions. The equations derived from CAO pattern of G-F-L model can be used to calculate energetic properties, volumetric properties and ordering degrees of the PAD AuCuI region and PAD boundary region, as well as corresponding average properties of the AuCulI phase. The results are consistent with experimental phenomena.
基金Project (11271121) supported by the National Natural Science Foundation of ChinaProject (11JJ2002) supported by the Natural Science Foundation of Hunan Province,China+1 种基金Project (11K038) supported by Key Laboratory of High Performance Computing and Stochastic Information Processing of Ministry of Education of ChinaProjects (2013GK3130,2014GK3090) supported by the Scientific and Techrnological Plan of Hunan Province,China
文摘The electronic structures,chemical bonding,elastic and optical properties of the ternary stannide phase Na2MgSn were investigated by using density-fimctional theory(DFT) within generalized gradient approximation(GGA).The calculated energy band structures show that Na2MgSn is an indirect semiconductor material with a narrow band gap 0.126 eV.The density of state(DOS)and the partial density of state(PDOS) calculations show that the DOS near the Fermi level is mainly from the Na 2p,Mg 3p and Sn5 p states.Population analysis suggests that there are strongly bonded Mg-Sn honeycomb layers in Na2MgSn.Basic physical properties,such as lattice constant,bulk modulus,shear modulus,elastic constants c(ij) were calculated.The elastic modulus E and Poisson ratio v were also predicted.The results show that Na2MgSn is mechanically stable soft material and behaves in a brittle manner.Detailed analysis of all optical functions reveals that Na2MgSn is a better dielectric material,and reflectivity spectra show that Na2MgSn promise as good coating materials in the energy regions 6.24-10.49 eV.
基金The Ph.D.Programs Foundation of Ministry of Education of China(No.20120092110053)
文摘The microstructure of asphalt is investigated by atomic force microscopy (AFM). In order to analyze the impacts of asphalt types on microstructures, two neat asphalts with different penetration grades (50# and 70#) and one styrene-butadiene-styrene (SBS) modified asphalt are chosen. The influence of short-term aging is also studied. Based on the knowledge of asphalt's microproperties, the relationship between microstructures and healing property is analyzed. The results indicate that the microstructures of three asphalts are quite different and the effects of aging on the surface characteristics for different asphalts are also different. It is proposed that the bee structure is a type of wax crystal and it has a close relationship with the "bridge-healing" mechanism. The findings may reveal the formation mechanism of microstructure and the healing property for asphalts.
基金Project(51074087)supported by the National Natural Science Foundation of ChinaProject(201102088)supported by the Natural Science Foundation of Liaoning Province,China+1 种基金Project(LJQ2011065)supported by Liaoning Excellent Talents in University,ChinaProject(2010921096)supported by Liaoning Baiqianwan Talents Program,China
文摘The electric pulse modification (EP, EPM) of liquid metal is a novel method for grain refinement. The structure of EP-modified Al-5%Cu melt was characterized by high-temperature X-ray diffractometry. The results show that the Cu-containing Al clusters remarkably increase in the EP-modified melt, furthermore, these clusters in that case tend to contract due to the decrease of relevant atomic radius and the co-ordination number. This kind of liquid-phase structure leads to a more homogeneous Cu-rich phase distribution in the final solidification structure. Differential scanning calorimetry (DSC) tests indicate that the solidification super-cooling degree of the EP-modified liquid phase is 2.36 times that of the unmodified. These facts suggest that the atom cluster changes in EP-modified Al-5%Cu melt would disagree with that by EPM model previously proposed in liquid pure metal.
基金International Cooperation Project of the Ministry of Science and Technology of China(No.2014DFA50320)National Natural Science Foundation of China(Nos.51674226,51574207,51574206,51274175)+1 种基金International Science and Technology Cooperation Project of Shanxi Province(No.2015081041)Research Project Supported by Shanxi Scholarship Council of China(No.2016-Key 2)
文摘The structural stability, elastic and electronic properties under pressure at 0 K for β-Ti have been investigated by per-forming first-principles calculations. With the increase of pressure, the structure of β-Ti becomes stabler, which is further con-firmed by the calculation for density of state (DOS). The phase transition pressure of is about 64. 3 GPa, which is consist-ent with other theoretical predictions (63. 7 GPa) and the experimental result (50 GPa). The pressure dependence of elastic constants shows that the low-pressure limit for a mechanically stable β-Ti is about 50 GPa with low Young?s modulus value of about 30. 01 GPa, which approaches the value of a human bone (30 GPa). In addition, the pressure dependence of bulk modu-lus B, shear modulus G, Young’s modulus E,Poisson’s ratio σ,aggregate sound velocities,and ductility/brittleness under different pressures were also discussed. B, G and E ascend monotonously with increasing pressure, while a descends. β-Ti re-mains ductile by analysis of B/G under considered pressures.
文摘[Objective] The research aimed to provide reference for increasing the genetic transformation efficiency of Ginkgo biloba mediated by Agrobacterium.[Method] Taking the mature embryos of Ginkgo biloba seeds as explants,after 48 hours' pre-cultivation on MS medium in the absence of phytohormone,GUS gene was transmitted into embryos of Ginkgo biloba mediated by three kinds of Agrobacterium.Transient expression of GUS gene activity was observed through histochemical staining,and the influencing factors of the expression of GUS gene were analyzed.And the expression vector of 1-deoxy-D-xylulose-5-phosphate reductoisomerase in the biosynthesis approach of biobalide precursor of Ginkgo biloba was constructed.[Result] A more suitable genetic transformation scheme was obtained as follows:taking embryos of Ginkgo biloba as explants,using EHA105 Agrobacterium with pCAMBIA1304+ for infection,co-culture for 3 days and GUS staining.The results showed that transient expression rate of GUS after transformation was higher.[Conclusion] The research provide a more effective method for further study on the transgene of Ginkgo biloba.
基金Project(20131083) supported by the Doctoral Starting up Foundation of Liaoning Province,ClhinaProject(LT201304) supported by the Program for Liaoning Innovative Research Team in University,ChinaProject(2013201018) supported by the Key Technologies Research and Development Program of Liaoning Province,China
文摘The structural stability, electronic structures, elastic properties and thermodynamic properties of the main binary phases Mg_(17)Al_(12), Al_2Ca, Mg_2 Sn and Mg_2 Ca in Mg-Al-Ca-Sn alloy were determined from the first-principles calculation. The calculated lattice parameters are in good agreement with the experimental and literature values. The calculated heats of formation and cohesive energies show that Al_2Ca has the strongest alloying ability and structural stability. The densities of states(DOS), Mulliken electron occupation number, metallicity and charge density difference of these compounds are given. The elastic constants of Mg_(17)Al_(12), Al_2Ca, Mg_2 Sn and Mg_2 Ca phases are calculated, and the bulk moduli, shear moduli, elastic moduli and Poisson ratio are derived. The calculations of thermodynamic properties show that the Gibbs free energies of Al_2Ca and Mg_2 Sn are lower than that of Mg_(17)Al_(12), which indicates that Al_2Ca and Mg_2 Sn are more stable than Mg_(17)Al_(12) phase. Hence, the heat resistance of Mg-Al-based alloys can be improved by adding Ca and Sn additions.
基金Project (200805321032) supported by Doctoral Fund of Ministry of Education of ChinaProject (51071065) supported by the National Natural Science Foundation of ChinaProject (71075003) supported by the Science Fund of State Key Laboratory of Advanced Design and Manufacturing for Vehicle Body, China
文摘The electronic structures and mechanical properties of Al4Sr, Mg2Sr and Mg23Sr6 phases were determined by the use of first-principles calculations. The calculated heat of formation and cohesive energy indicate that Al4Sr has the strongest alloying ability as well as the highest structural stability. The elastic parameters were calculated, and then the bulk modulus, shear modulus, elastic modulus and Poisson ratio were derived. The ductility and plasticity were discussed. The results show that Al4Sr and Mg2Sr phases both are ductile, on the contrary, Mg23Sr6 is brittle, and among the three phases, Mg2Sr is a phase with the best plasticity.