Objective: To observe whether there is evidence for vascular channel formation by osteosarcoma cellsin vitro and to illustrate mechanism of vasculogenic mimicry in osteosarcoma.Methods: Osteosarcoma cell lines (U-2OS)...Objective: To observe whether there is evidence for vascular channel formation by osteosarcoma cellsin vitro and to illustrate mechanism of vasculogenic mimicry in osteosarcoma.Methods: Osteosarcoma cell lines (U-2OS) were tested for their ability to form tubular networks in three-dimensional culture containing type I collagen. The structures of the tubular networks were observed under a phase contrast microscope and an electron microscope.Results: Observation under light microscopy and electron microscopy showed that high aggressive osteosarcoma cells line (U-2OS) formed networks containing channels when grown in three-dimensional culture containing type I collagen in the absence of endothelial cells or fibroblasts.Conclusion: These observations strongly suggest that aggressive osteosarcoma cells may generate vascular channels that facilitate tumor perfusion independent of tumor angiogenesis and have the ability of vasculogenic mimicry. Key words osteosarcoma cells line - vasculogenesis mimicry - angiogenesis - 3-dimensional cultures This study was supported in part by the National Natural Sciences Foundation of China (No. 30271314).展开更多
As a result of environmental degradation,urban green space has become a key issue for urban sustainable development.This paper takes Liaoyang City in Northeast China as an example to develop green space planning using...As a result of environmental degradation,urban green space has become a key issue for urban sustainable development.This paper takes Liaoyang City in Northeast China as an example to develop green space planning using the computational fluid dynamics (CFD) model,landscape ecological principles and Geographical Information System (GIS).Based on the influencing factors of topography,building density and orientation,Shou Mountain,Longding Mountain and the Taizi River were selected as the urban ventilation paths to promote wind and oxygen circulation.Oxygen concentration around the green spaces gradually decreased with wind speed increase and wind direction change.There were obvious negative correlation relationships between the oxygen dispersion concentration and urban layout factors such as the building plot ratio and building density.Comparison with the field measurements found that there was significant correlation relationship between simulated oxygen concentration and field measurements (R 2=0.6415,p<0.001),moreover,simulation precision was higher than 92%,which indicated CFD model was effective for urban oxygen concentration simulation.Only less than 10% areas in Liaoyang City proper needed more green space urgently to improve oxygen concentration,mainly concentrated in Baitai and west Wensheng districts.Based on land-scape ecology principle,green space planning at different spatial scales were proposed to create a green space network system for Liaoyang City,including features such as green wedges,green belts and parks.Totally,about 2012 ha of green space need to be constructed as oxygen sources and ventilation paths.Compared with the current green space pattern,proposed green space planning could improve oxygen concentration obviously.The CFD model and research results in this paper could provide an effective way and theory support for sustainable development of urban green space.展开更多
Permafrost degradation is prevalent on the Qinghai-Tibet Plateau.This may lead to changes in water and heat transition in soils and thus affect the structure and function of ecosystems.In this paper,using the measured...Permafrost degradation is prevalent on the Qinghai-Tibet Plateau.This may lead to changes in water and heat transition in soils and thus affect the structure and function of ecosystems.In this paper,using the measured data of alpine steppe in Wudaoliang assessed the model performance in simulating soil freezing and thawing processes.Comparison of the simulated results by simultaneous heat and water(SHAW) model to the measured data showed that SHAW model performed satisfactorily.Based on analyzing the simulated and predicted results,two points were obtained:(1) freezing and thawing of the active layer proceeded both from the soil surface downward.Compared with the freezing process,the thawing process was slower.The freezing period persisted in the surface layer(4 cm depth) for about 5 months;(2) in the next 50 years,frozen period would be shorten about 20 days in the top 100 cm depth while the thawing would start earlier 40 days than present.Soil water storage in the 0-60 cm would decrease by 22% averagely,especially from June to August when the vegetation is at the dominating water consumed stage.Therefore,this kind of permafrost degradation as active layer freezing and thawing processes changes will reduce soil water content and thus influence those ecosystems above it.展开更多
Based on the Terrestrial Ecosystem Model(TEM 5.0), together with the data of climate(temperature, precipitation and solar radiation) and environment(grassland vegetation types, soil texture, altitude, longitude and la...Based on the Terrestrial Ecosystem Model(TEM 5.0), together with the data of climate(temperature, precipitation and solar radiation) and environment(grassland vegetation types, soil texture, altitude, longitude and latitude, and atmospheric CO2 concentration data), the spatiotemporal variations of carbon storage and density, and their controlling factors were discussed in this paper. The results indicated that:(1) the total carbon storage of China's grasslands with a total area of 394.93×104 km2 was 59.47 Pg C. Among them, there were 3.15 Pg C in vegetation and 56.32 Pg C in soil carbon. China's grasslands covering 7.0–11.3% of the total world's grassland area had 1.3–11.3% of the vegetation carbon and 9.7–22.5% of the soil carbon in the world grasslands. The total carbon storage increased from 59.13 to 60.16 Pg C during 1961–2013 with an increasing rate of 19.4 Tg C yr^(-1).(2) The grasslands in the Qinghai-Tibetan Plateau contributed most to the total carbon storage during 1961–2013, accounting for 63.2% of the total grassland carbon storage, followed by Xinjiang grasslands(15.8%) and Inner Mongolia grasslands(11.1%).(3) The vegetation carbon storage showed an increasing trend, with the average annual growth rate of 9.62 Tg C yr^(-1) during 1961–2013, and temperature was the main determinant factor, explaining approximately 85% of its variation. The vegetation carbon storage showed an increasing trend in most grassland regions, however, a decreasing trend in the central grassland in the southern China, the western and central parts of the Inner Mongolian grasslands as well as some parts on the Qinghai-Tibetan Plateau. The soil carbon storage showed a significantly increasing trend with a rate of 7.96 Tg C yr^(-1), which resulted from the interaction of more precipitation and low temperature in the 1980 s and 1990 s. Among them, precipitation was the main determinant factor of increasing soil carbon increases of China's grasslands.展开更多
Aboveground biomass in grasslands of the Qinghai-Tibet Plateau has displayed an overall increasing trend during 2003–2016, which is profoundly influenced by climate change. However, the responses of different biomes ...Aboveground biomass in grasslands of the Qinghai-Tibet Plateau has displayed an overall increasing trend during 2003–2016, which is profoundly influenced by climate change. However, the responses of different biomes show large discrepancies, in both size and magnitude. By applying partial least squares regression, we calculated the correlation between peak aboveground biomass and mean monthly temperature and monthly total precipitation in the preceding 12 months for three different grassland types(alpine steppe, alpine meadow, and temperate steppe) on the central and eastern Qinghai-Tibet Plateau. The results showed that mean temperature in most preceding months was positively correlated with peak aboveground biomass of alpine meadow and alpine steppe, while mean temperature in the preceding October and February to June was significantly negatively correlated with peak aboveground biomass of temperate steppe. Precipitation in all months had a promoting effect on biomass of alpine meadow, but its correlations with biomass of alpine steppe and temperate steppe were inconsistent. It is worth noting that, in a warmer, wetter climate, peak aboveground biomass of alpine meadow would increase more than that of alpine steppe, while that of temperate steppe would decrease significantly, providing support for the hypothesis of conservative growth strategies by vegetation in stressed ecosystems.展开更多
基金This study was supported in part by the National Natural Sciences Foundation of China(No.30271314).
文摘Objective: To observe whether there is evidence for vascular channel formation by osteosarcoma cellsin vitro and to illustrate mechanism of vasculogenic mimicry in osteosarcoma.Methods: Osteosarcoma cell lines (U-2OS) were tested for their ability to form tubular networks in three-dimensional culture containing type I collagen. The structures of the tubular networks were observed under a phase contrast microscope and an electron microscope.Results: Observation under light microscopy and electron microscopy showed that high aggressive osteosarcoma cells line (U-2OS) formed networks containing channels when grown in three-dimensional culture containing type I collagen in the absence of endothelial cells or fibroblasts.Conclusion: These observations strongly suggest that aggressive osteosarcoma cells may generate vascular channels that facilitate tumor perfusion independent of tumor angiogenesis and have the ability of vasculogenic mimicry. Key words osteosarcoma cells line - vasculogenesis mimicry - angiogenesis - 3-dimensional cultures This study was supported in part by the National Natural Sciences Foundation of China (No. 30271314).
基金Under the auspices of National Key Technology Research and Development Program of China (No.2008BAJ10B01-01)National Natural Science Foundation of China (No.40801069)
文摘As a result of environmental degradation,urban green space has become a key issue for urban sustainable development.This paper takes Liaoyang City in Northeast China as an example to develop green space planning using the computational fluid dynamics (CFD) model,landscape ecological principles and Geographical Information System (GIS).Based on the influencing factors of topography,building density and orientation,Shou Mountain,Longding Mountain and the Taizi River were selected as the urban ventilation paths to promote wind and oxygen circulation.Oxygen concentration around the green spaces gradually decreased with wind speed increase and wind direction change.There were obvious negative correlation relationships between the oxygen dispersion concentration and urban layout factors such as the building plot ratio and building density.Comparison with the field measurements found that there was significant correlation relationship between simulated oxygen concentration and field measurements (R 2=0.6415,p<0.001),moreover,simulation precision was higher than 92%,which indicated CFD model was effective for urban oxygen concentration simulation.Only less than 10% areas in Liaoyang City proper needed more green space urgently to improve oxygen concentration,mainly concentrated in Baitai and west Wensheng districts.Based on land-scape ecology principle,green space planning at different spatial scales were proposed to create a green space network system for Liaoyang City,including features such as green wedges,green belts and parks.Totally,about 2012 ha of green space need to be constructed as oxygen sources and ventilation paths.Compared with the current green space pattern,proposed green space planning could improve oxygen concentration obviously.The CFD model and research results in this paper could provide an effective way and theory support for sustainable development of urban green space.
基金supported by the National Basic Research Program of China(Grant No.2005CB422005)the National Basic S&T Project of China(Grant No.2006FY110200)China Postdoctoral Science Foundation(Grant No.20090460506)
文摘Permafrost degradation is prevalent on the Qinghai-Tibet Plateau.This may lead to changes in water and heat transition in soils and thus affect the structure and function of ecosystems.In this paper,using the measured data of alpine steppe in Wudaoliang assessed the model performance in simulating soil freezing and thawing processes.Comparison of the simulated results by simultaneous heat and water(SHAW) model to the measured data showed that SHAW model performed satisfactorily.Based on analyzing the simulated and predicted results,two points were obtained:(1) freezing and thawing of the active layer proceeded both from the soil surface downward.Compared with the freezing process,the thawing process was slower.The freezing period persisted in the surface layer(4 cm depth) for about 5 months;(2) in the next 50 years,frozen period would be shorten about 20 days in the top 100 cm depth while the thawing would start earlier 40 days than present.Soil water storage in the 0-60 cm would decrease by 22% averagely,especially from June to August when the vegetation is at the dominating water consumed stage.Therefore,this kind of permafrost degradation as active layer freezing and thawing processes changes will reduce soil water content and thus influence those ecosystems above it.
基金supported by the Strategic Priority Research Program–Climate Change:Carbon Budget and Related Issues of the Chinese Academy of Sciences(Grant No.XDA-05050408)
文摘Based on the Terrestrial Ecosystem Model(TEM 5.0), together with the data of climate(temperature, precipitation and solar radiation) and environment(grassland vegetation types, soil texture, altitude, longitude and latitude, and atmospheric CO2 concentration data), the spatiotemporal variations of carbon storage and density, and their controlling factors were discussed in this paper. The results indicated that:(1) the total carbon storage of China's grasslands with a total area of 394.93×104 km2 was 59.47 Pg C. Among them, there were 3.15 Pg C in vegetation and 56.32 Pg C in soil carbon. China's grasslands covering 7.0–11.3% of the total world's grassland area had 1.3–11.3% of the vegetation carbon and 9.7–22.5% of the soil carbon in the world grasslands. The total carbon storage increased from 59.13 to 60.16 Pg C during 1961–2013 with an increasing rate of 19.4 Tg C yr^(-1).(2) The grasslands in the Qinghai-Tibetan Plateau contributed most to the total carbon storage during 1961–2013, accounting for 63.2% of the total grassland carbon storage, followed by Xinjiang grasslands(15.8%) and Inner Mongolia grasslands(11.1%).(3) The vegetation carbon storage showed an increasing trend, with the average annual growth rate of 9.62 Tg C yr^(-1) during 1961–2013, and temperature was the main determinant factor, explaining approximately 85% of its variation. The vegetation carbon storage showed an increasing trend in most grassland regions, however, a decreasing trend in the central grassland in the southern China, the western and central parts of the Inner Mongolian grasslands as well as some parts on the Qinghai-Tibetan Plateau. The soil carbon storage showed a significantly increasing trend with a rate of 7.96 Tg C yr^(-1), which resulted from the interaction of more precipitation and low temperature in the 1980 s and 1990 s. Among them, precipitation was the main determinant factor of increasing soil carbon increases of China's grasslands.
基金National Key R&D Program of China,No.2018YFA0606102National Natural Science Foundation of China,No.41771056National Key Technology Support Program,No.2012BAH31B02
文摘Aboveground biomass in grasslands of the Qinghai-Tibet Plateau has displayed an overall increasing trend during 2003–2016, which is profoundly influenced by climate change. However, the responses of different biomes show large discrepancies, in both size and magnitude. By applying partial least squares regression, we calculated the correlation between peak aboveground biomass and mean monthly temperature and monthly total precipitation in the preceding 12 months for three different grassland types(alpine steppe, alpine meadow, and temperate steppe) on the central and eastern Qinghai-Tibet Plateau. The results showed that mean temperature in most preceding months was positively correlated with peak aboveground biomass of alpine meadow and alpine steppe, while mean temperature in the preceding October and February to June was significantly negatively correlated with peak aboveground biomass of temperate steppe. Precipitation in all months had a promoting effect on biomass of alpine meadow, but its correlations with biomass of alpine steppe and temperate steppe were inconsistent. It is worth noting that, in a warmer, wetter climate, peak aboveground biomass of alpine meadow would increase more than that of alpine steppe, while that of temperate steppe would decrease significantly, providing support for the hypothesis of conservative growth strategies by vegetation in stressed ecosystems.