A sulfate reducing bacteria was isolated from mining sewage of Daqing Oilfield by Hungate anaerobic technology. Physiological-biochemical analysis showed that the strain could utilize polyacrylamide as sole carbon and...A sulfate reducing bacteria was isolated from mining sewage of Daqing Oilfield by Hungate anaerobic technology. Physiological-biochemical analysis showed that the strain could utilize polyacrylamide as sole carbon and nitrogen source. The sequence analysis of 16S rDNA illustrated that the similarity of F8 and Desulfovibrio desulfuricans (AF192153) was 99%, and the similarity sequence of dissimilatory sulfite reductase gene (DSR) cloned from the strain and Desulfovibrio desulfuricans (AF273034) was 98%. Their phylogenitic analysis was basically anastomosed, and thus temporarily named as Desulfovibrio desulfuricans F8. The DSR cloned from F8 strain was 2740 bp in length consisting of three ORF, DSRA, DSRB and DSRD as a single operon (DSRABD) regulated by the same operator. DSRA contained typical conservative box of sulfate—sulfite reducing enzyme (SiteⅠand SiteⅡ), which could bind siroheme and [Fe4S4]. DSRB retained a [Fe4S4] binding site, with an uncomplimentary structure for siroheme binding. There was no conservative box in DSRD. Sequence analysis of DSR will provide a theoretical basis for quantitative detection, metabolic pathway modification through gene engineering, and sulfate reducing bacteria (SRB) suppression.展开更多
The issue of the building materials biocorrosion has a significant economic dimension because it results in the costly repair. The start and the course of corrosion are conditioned by many factors which undoubtedly in...The issue of the building materials biocorrosion has a significant economic dimension because it results in the costly repair. The start and the course of corrosion are conditioned by many factors which undoubtedly include biological effects like the influence of vegetation and microorganisms causing the so called microbial corrosion. Microorganisms have also a considerable share in the decay and degradation of different building materials. The activity of sulphuratum is the keystone of many processes in nature and in industry. The sulphuric bacteria oxidize hydrogen sulphide produced by sulphate-reducing bacteria into sulphuric acid resulting in the acid or sulphate corrosion of cement stone. The paper is aimed on evaluation of porosity and roughness of concrete samples surface as elementary characteristics of microbial corrosion. After 4 months exposure to the real conditions in sewers deposition the changes were observed by confocal laser microscopy and consequently analyzed and interpreted.展开更多
The foaming phenomenon of N-methyldiethanolamine(MDEA) solution used in desulfurization process occurs frequently in the natural-gas purification plant. The foaming phenomenon has a strong impact on operation of the p...The foaming phenomenon of N-methyldiethanolamine(MDEA) solution used in desulfurization process occurs frequently in the natural-gas purification plant. The foaming phenomenon has a strong impact on operation of the process unit. The salt impurities are the main reason for causing the foaming of MDEA solution, so the full analysis of salt impurities is necessary. A method for comprehensive analysis of salt impurities in MDEA solution used in desulfurization process was established. Anions and non-metallic cations of MDEA solution were determined by different conditions of ion chromatograph, respectively. Metallic cations of the solution were detected by atomic absorption spectrophotometer with the N2O-C2H2 flame absorption. The analytical results of salt impurities in the desulfurization solution can provide a theoretical basis for an accurate analysis of the factors affecting the foaming of MDEA to unveil further control measures.展开更多
基金Sponsored by the National Basic Research and Development (973) Program of China(Grant No.2004CB418505)
文摘A sulfate reducing bacteria was isolated from mining sewage of Daqing Oilfield by Hungate anaerobic technology. Physiological-biochemical analysis showed that the strain could utilize polyacrylamide as sole carbon and nitrogen source. The sequence analysis of 16S rDNA illustrated that the similarity of F8 and Desulfovibrio desulfuricans (AF192153) was 99%, and the similarity sequence of dissimilatory sulfite reductase gene (DSR) cloned from the strain and Desulfovibrio desulfuricans (AF273034) was 98%. Their phylogenitic analysis was basically anastomosed, and thus temporarily named as Desulfovibrio desulfuricans F8. The DSR cloned from F8 strain was 2740 bp in length consisting of three ORF, DSRA, DSRB and DSRD as a single operon (DSRABD) regulated by the same operator. DSRA contained typical conservative box of sulfate—sulfite reducing enzyme (SiteⅠand SiteⅡ), which could bind siroheme and [Fe4S4]. DSRB retained a [Fe4S4] binding site, with an uncomplimentary structure for siroheme binding. There was no conservative box in DSRD. Sequence analysis of DSR will provide a theoretical basis for quantitative detection, metabolic pathway modification through gene engineering, and sulfate reducing bacteria (SRB) suppression.
文摘The issue of the building materials biocorrosion has a significant economic dimension because it results in the costly repair. The start and the course of corrosion are conditioned by many factors which undoubtedly include biological effects like the influence of vegetation and microorganisms causing the so called microbial corrosion. Microorganisms have also a considerable share in the decay and degradation of different building materials. The activity of sulphuratum is the keystone of many processes in nature and in industry. The sulphuric bacteria oxidize hydrogen sulphide produced by sulphate-reducing bacteria into sulphuric acid resulting in the acid or sulphate corrosion of cement stone. The paper is aimed on evaluation of porosity and roughness of concrete samples surface as elementary characteristics of microbial corrosion. After 4 months exposure to the real conditions in sewers deposition the changes were observed by confocal laser microscopy and consequently analyzed and interpreted.
基金Financial support received from the Major National Science and Technology Projects of China (No. 2011ZX05017)SWPU Science & Technology Innovation Youth Team for Pollution Control of Oil & Gas Fields (No. 2013XJZT003)
文摘The foaming phenomenon of N-methyldiethanolamine(MDEA) solution used in desulfurization process occurs frequently in the natural-gas purification plant. The foaming phenomenon has a strong impact on operation of the process unit. The salt impurities are the main reason for causing the foaming of MDEA solution, so the full analysis of salt impurities is necessary. A method for comprehensive analysis of salt impurities in MDEA solution used in desulfurization process was established. Anions and non-metallic cations of MDEA solution were determined by different conditions of ion chromatograph, respectively. Metallic cations of the solution were detected by atomic absorption spectrophotometer with the N2O-C2H2 flame absorption. The analytical results of salt impurities in the desulfurization solution can provide a theoretical basis for an accurate analysis of the factors affecting the foaming of MDEA to unveil further control measures.
