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茶皂素对奶牛瘤胃原虫区系的影响 被引量:4
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作者 赵士萍 严淑红 +5 位作者 蒋琦晖 常肖肖 王炳 方洛云 蒋林树 熊本海 《动物营养学报》 CAS CSCD 北大核心 2017年第2期620-627,共8页
本研究旨在研究茶皂素对奶牛瘤胃原虫区系的影响。选取12头健康荷斯坦奶牛[体重(550±30)kg,产奶量35 kg/(头·d),胎次2~4],随机分为4组,每组3头,均饲喂基础饲粮,分别于晨饲前灌服0(对照)、20、30、40 g/(头·d)的茶皂素。... 本研究旨在研究茶皂素对奶牛瘤胃原虫区系的影响。选取12头健康荷斯坦奶牛[体重(550±30)kg,产奶量35 kg/(头·d),胎次2~4],随机分为4组,每组3头,均饲喂基础饲粮,分别于晨饲前灌服0(对照)、20、30、40 g/(头·d)的茶皂素。试验期49 d,其中预试期14 d,正试期35 d,正试期内每7 d于晨饲前1 h用口腔采样器采集瘤胃液。采用聚丙烯酰氨凝胶电泳(DGGE)结合18S r DNA序列分析技术研究瘤胃原虫区系的变化。结果表明:1)添加茶皂素可以选择性地抑制瘤胃中原虫的生长。2)与对照组相比,30、40 g/(头·d)茶皂素组的丰富度指数和香农多样性指数都显著降低(P<0.05),优势度指数显著增加(P<0.05),但是均一性指数无显著变化(P>0.05);茶皂素显著减少了瘤胃中前庭亚纲内毛目的原虫数量。综上所述,饲粮中添加30、40 g/(头·d)茶皂素均可抑制奶牛瘤胃原虫的生长,并且降低瘤胃原虫的多样性。 展开更多
关键词 奶牛 原虫区系 茶皂素
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Nematode Faunal Response to Grassland Degradation in Horqin Sandy Land 被引量:9
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作者 LIANG Wen-Ju ZHONG Shuang +2 位作者 HUA Jian-Feng CAO Cheng-You JIANG Yong 《Pedosphere》 SCIE CAS CSCD 2007年第5期611-618,共8页
The responses of soil nematode communities to grassland degradation were studied under undegraded grassland (UG), degraded grassland (DG),and improved grassland (IG),in Horqin Sandy Land,Inner Mongolia,Northeast China... The responses of soil nematode communities to grassland degradation were studied under undegraded grassland (UG), degraded grassland (DG),and improved grassland (IG),in Horqin Sandy Land,Inner Mongolia,Northeast China.Soil samples were collected at depths of 0-10,10-20,and 20-30 cm.Total organic carbon (TOC) and total nitrogen (TN) exhibited positive effects on the total number of nematodes and trophic groups.Significant treatment effects were found in the total number of nematodes,plant parasites,and omnivores-predators.Measures taken in the improved grassland could improve the number of omnivore-predators,especially in the deeper soil layers.Nematode richness was lower in the DG treatment than in the IG and UG treatments.The food web structure index (SI) was significantly higher in the UG and IG treatments than in the DG treatment.A higher SI suggested a food web with more trophic linkages and relatively healthy ecosystems. 展开更多
关键词 functional groups grassland degradation Horqin Sandy Land soil nematodes
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