The fabrication of a new type of one-dimensional Au-Ag porous nanotube（NPT） structure was presented based on a facile combination of nanocrystal growth and surface modification.Ag nanowires with various diameters we...The fabrication of a new type of one-dimensional Au-Ag porous nanotube（NPT） structure was presented based on a facile combination of nanocrystal growth and surface modification.Ag nanowires with various diameters were firstly served as the chemical plating templates via a polyol-process.Then,one-dimensional（1D） Au-Ag porous nanostructures with tailored structural features could be prepared by controlling the individual steps involved in this process,such as nanowire growth,surface modification,thermal diffusion,and dealloying.Structural characterizations reveal these Au-Ag porous nanotubes,non-porous nanotubes and porous nanowires possess novel nano-architectures with multimodal open porosity and excellent structural continuity and integrity,which make them particularly desirable as novel 1D nanocarriers for biomedical,drug delivery and sensing applications.展开更多
AIM:To identify the novel methylation-silenced gene pentraxin 3(PTX3) in esophageal squamous cell carcinoma(ESCC).METHODS:PTX3 mRNA expression was examined in six human ESCC cell lines,one human immortalized normal es...AIM:To identify the novel methylation-silenced gene pentraxin 3(PTX3) in esophageal squamous cell carcinoma(ESCC).METHODS:PTX3 mRNA expression was examined in six human ESCC cell lines,one human immortalized normal esophageal epithelial cell line,primary ESCC tumor tissue,and paired adjacent nontumor tissue using reverse transcription polymerase chain reaction(RTPCR).Semi-quantitative immunohistochemistry was used to examine cellular localisation and protein levels.Methylation specific PCR and bisulphite genomic sequencing were employed to investigate the methylation of the candidate gene.RESULTS:In the majority of ESCC cell lines,we found that PTX3 expression was down-regulated due to gene promoter hypermethylation,which was further confirmed by bisulphite genomic sequencing.Demethylation treatment with 5-aza-2'-deoxycytidine restored PTX3 mRNA expression in ESCC cell lines.Methylation was more common in tumor tissues(85%) than in adjacent nontumor tissues(25%)(P < 0.01).CONCLUSION:PTX3 is down-regulated through promoter hypermethylation in ESCC,and could potentially serve as a biomarker of ESCC.展开更多
Although the pathogenesis of cardio-cerebrovascular disease （CCVD） is multifactorial, an increasing number of experimental and clinical studies have highlighted the importance of histone deacetylase （HDAC）-mediate...Although the pathogenesis of cardio-cerebrovascular disease （CCVD） is multifactorial, an increasing number of experimental and clinical studies have highlighted the importance of histone deacetylase （HDAC）-mediated epigenetic processes in the development of cardio-cerebrovascular injury. HDACs are a family of enzymes to balance the acetylation activities of histone acetyltransferases on chromatin remodeling and play essential roles in regulating gene transcription. To date, 18 mammalian HDACs are identified and grouped into four classes based on similarity to yeast orthologs. The zinc-dependent HDAC family currently consists of 11 members divided into three classes （class I, II, and IV） on the basis of structure, sequence homology, and domain organization. In comparison, class III HDACs （also known as the sirtuins） are composed of a family of NAD＋-dependent protein-modifying enzymes related to the Sir2 gene. HDAC inhibitors are a group of compounds that block HDAC activities typically by binding to the zinc-containing catalytic domain of HDACs and have displayed an- ti-inflammatory and antifibrotic effects in the cardio-cerebrovascular system. In this review, we summarize the current knowledge about classifications, functions of HDACs and their roles and regulatory mechanisms in the cardio-cerebrovascular system. Pharmacological tar- geting of HDAC-mediated epigenetic processes may open new therapeutic avenues for the treatment of CCVD.展开更多
基金Project (2012CB932800) supported by the National Basic Research Program of ChinaProject (2012M521330) supported by China Postdoctoral Science Foundation
文摘The fabrication of a new type of one-dimensional Au-Ag porous nanotube（NPT） structure was presented based on a facile combination of nanocrystal growth and surface modification.Ag nanowires with various diameters were firstly served as the chemical plating templates via a polyol-process.Then,one-dimensional（1D） Au-Ag porous nanostructures with tailored structural features could be prepared by controlling the individual steps involved in this process,such as nanowire growth,surface modification,thermal diffusion,and dealloying.Structural characterizations reveal these Au-Ag porous nanotubes,non-porous nanotubes and porous nanowires possess novel nano-architectures with multimodal open porosity and excellent structural continuity and integrity,which make them particularly desirable as novel 1D nanocarriers for biomedical,drug delivery and sensing applications.
基金Supported by National High Technology Research and Development Program of China (863 Program),No. 2007AA02Z4Z4China Postdoctoral Science Foundation,No. 20090460394Beijing Municipal Natural Science Foundation,No. 7072022
文摘AIM:To identify the novel methylation-silenced gene pentraxin 3(PTX3) in esophageal squamous cell carcinoma(ESCC).METHODS:PTX3 mRNA expression was examined in six human ESCC cell lines,one human immortalized normal esophageal epithelial cell line,primary ESCC tumor tissue,and paired adjacent nontumor tissue using reverse transcription polymerase chain reaction(RTPCR).Semi-quantitative immunohistochemistry was used to examine cellular localisation and protein levels.Methylation specific PCR and bisulphite genomic sequencing were employed to investigate the methylation of the candidate gene.RESULTS:In the majority of ESCC cell lines,we found that PTX3 expression was down-regulated due to gene promoter hypermethylation,which was further confirmed by bisulphite genomic sequencing.Demethylation treatment with 5-aza-2'-deoxycytidine restored PTX3 mRNA expression in ESCC cell lines.Methylation was more common in tumor tissues(85%) than in adjacent nontumor tissues(25%)(P < 0.01).CONCLUSION:PTX3 is down-regulated through promoter hypermethylation in ESCC,and could potentially serve as a biomarker of ESCC.
基金This study was supported by grants from the National 973 Basic Research Program of China，the National Nature Science Foundation of China，Foundation of Program for New Century Excellent Talents in University (NCET-11-0311) to Yi F，Program for Changjiang Scholars and Innovative Research Team in University，the Special Financial Grant from the China Postdoctoral Science Foundation，the China Postdoctoral Science Foundation，the Shandong Province Post-doctoral Innovation Foundation
文摘Although the pathogenesis of cardio-cerebrovascular disease （CCVD） is multifactorial, an increasing number of experimental and clinical studies have highlighted the importance of histone deacetylase （HDAC）-mediated epigenetic processes in the development of cardio-cerebrovascular injury. HDACs are a family of enzymes to balance the acetylation activities of histone acetyltransferases on chromatin remodeling and play essential roles in regulating gene transcription. To date, 18 mammalian HDACs are identified and grouped into four classes based on similarity to yeast orthologs. The zinc-dependent HDAC family currently consists of 11 members divided into three classes （class I, II, and IV） on the basis of structure, sequence homology, and domain organization. In comparison, class III HDACs （also known as the sirtuins） are composed of a family of NAD＋-dependent protein-modifying enzymes related to the Sir2 gene. HDAC inhibitors are a group of compounds that block HDAC activities typically by binding to the zinc-containing catalytic domain of HDACs and have displayed an- ti-inflammatory and antifibrotic effects in the cardio-cerebrovascular system. In this review, we summarize the current knowledge about classifications, functions of HDACs and their roles and regulatory mechanisms in the cardio-cerebrovascular system. Pharmacological tar- geting of HDAC-mediated epigenetic processes may open new therapeutic avenues for the treatment of CCVD.
