为了准确测定原料和产品中的总大豆异黄酮含量,建立了通过碱水解法将不稳定形式的大豆异黄酮(含丙二酰基和乙酰基)转化为稳定形式(含葡萄糖苷和苷元)的液相色谱-三重串联四极杆液质联用仪(liquid chromatography-triple tandem quadrupo...为了准确测定原料和产品中的总大豆异黄酮含量,建立了通过碱水解法将不稳定形式的大豆异黄酮(含丙二酰基和乙酰基)转化为稳定形式(含葡萄糖苷和苷元)的液相色谱-三重串联四极杆液质联用仪(liquid chromatography-triple tandem quadrupole liquid mass spectrometer,LC-MS/MS)测定方法,并进行了验证。去酰基化后总大豆异黄酮由大豆苷、黄豆苷、染料木苷、大豆苷元、黄豆苷元、染料木素这六种化合物组成。它们的线性范围分别为0.1~4.1μg/mL、0.11~4.36μg/mL、0.12~4.84μg/mL、0.1~4μg/mL、0.11~4.58μg/mL、0.11~4.37μg/mL。大豆异黄酮原料和产品药片的相对标准偏差(RSD)分别为4%和2%,原料和片剂中各成分的回收率均在90%~110%之间,且所有组分分析完成时间不超过10分钟。大豆异黄酮量的准确测定保证了大豆异黄酮类原料中规定的异黄酮量,以及产品标签上的正确异黄酮量。展开更多
The initiation and progression of cancer not only involves genetic abnormalities, but also epigenetic alterations, such as DNA methylation and histone modifications. Epigenetics refers to the heritable changes that do...The initiation and progression of cancer not only involves genetic abnormalities, but also epigenetic alterations, such as DNA methylation and histone modifications. Epigenetics refers to the heritable changes that do not involve any structural changes in the target gene, i.e., DNA sequence and protein sequence. Thus, these epigenetic aberrations are potentially reversible, allowing the malignant cells to revert to a state with more normal characteristics. The use of epigenetics is emerging as an effective and promising approach to treat cancer. Epigenetic drugs, which target two well- known epigenetic pathways, namely, DNA methyltransferases and histone deacetylases, are already being applied for the cancer treatment. In the current study, an overview regarding the under-standing of epigenetic alterations in the development of cancer and the current state of epigenetic drug discovery is provided.展开更多
A convenient and effective procedure was developed for the conversion of alcohol to olefin by tosylation and subsequent β-elimination promoted by silica gel in this study. Treatment of the alcohols with p-toluenesulf...A convenient and effective procedure was developed for the conversion of alcohol to olefin by tosylation and subsequent β-elimination promoted by silica gel in this study. Treatment of the alcohols with p-toluenesulfonyl chloride in pyridine at 0℃ af- fords tosylates which undergo β-elimination with silica gel in dichloromethane or chloroform at room temperature, yielding olefins with high productivity.展开更多
Objective： To investigate the anti-proliferative effect of histone deacetylases-1 （HDAC-1） knockdown in Hela cells. Methods： The HDAC-1 protein was knockdowned using siRNA. The expression of HDAC-1 was detected by...Objective： To investigate the anti-proliferative effect of histone deacetylases-1 （HDAC-1） knockdown in Hela cells. Methods： The HDAC-1 protein was knockdowned using siRNA. The expression of HDAC-1 was detected by Western blotting. Apoptosis was assessed by flow cytometry. The inhibition of cell growth was assesses by MTT assay. Results： HDAC-1 siRNA knockdowned the expression of HDAC-1 protein. HDAC siRNA inhibited the proliferation of Hela cells. HDAC- 1 siRNA induced apoptosis. Conclusion： HDAC-1 siRNA may inhibit the growth of Hela cells by inducing apoptosis.展开更多
Lymphomas enconlpass a group of malignancies that originate in the lymph nodes or other lymphoid tissues. Epigenetic modification, especially by histone deacetylase （HDACs）, plays a key role during the occurrence an...Lymphomas enconlpass a group of malignancies that originate in the lymph nodes or other lymphoid tissues. Epigenetic modification, especially by histone deacetylase （HDACs）, plays a key role during the occurrence and development of lymphomas. Consequently, HDAC inhibitors （HDACIs）, a class of gene expression-modulating drugs, have emerged as promising mechanism-based agents for the treatment of lymphomas. This review presents the rationale of HDAC inhibition, describes the epigenetic-based mechanisms of action of HDACIs, discusses their clinical efficiency, and summarizes the current and future developments in this field.展开更多
The sea cucumber, Apostichopusjaponicus, undergoes aestivation to improve survival during periods of high-temperature. During aestivation, the metabolic rate is depressed to reduce the consumption of reserved energy. ...The sea cucumber, Apostichopusjaponicus, undergoes aestivation to improve survival during periods of high-temperature. During aestivation, the metabolic rate is depressed to reduce the consumption of reserved energy. We evaluated the role of epigenetic modification on global gene silencing during metabolic rate depression in the sea cucumber. We compared the expression of epigenetic modifiers in active and aestivating sea cucumbers. The expression of three genes involved in DNA methylation and chromatin remodeling （DNA （cytosine-5）-methyltransferase l, Methyl-CpG-binding domain protein 2）, and Chromodomain-helicase-DNA-binding protein 5） was significantly higher during aestivation （Days 20 and 40）. Similarly, we observed an increase in the expression of genes involved in histone acetylation （Histone deacetylase 3） and Histone-binding protein RBBP4） during the early （Days 5 and 10） and late phases （Days 20 and 40） of aestivation. There was no change in the expression of KAT2B, a histone acetyltransferase. However, the expression of histone methylation associated modifiers （Histone-arginine methyltransferase CARMER and Histone-lysine N-methyltransferase MLL5） was significantly higher after 5 d in the aestivating group. The results suggest that the expression of epigenetic modifiers involved in DNA methylation, chromatin remodeling, histone acetylation, and histone methylation is upregulated during aestivation. We hypothesize that these changes regulate global gene silencing during aestivation in A. japonicus.展开更多
文摘为了准确测定原料和产品中的总大豆异黄酮含量,建立了通过碱水解法将不稳定形式的大豆异黄酮(含丙二酰基和乙酰基)转化为稳定形式(含葡萄糖苷和苷元)的液相色谱-三重串联四极杆液质联用仪(liquid chromatography-triple tandem quadrupole liquid mass spectrometer,LC-MS/MS)测定方法,并进行了验证。去酰基化后总大豆异黄酮由大豆苷、黄豆苷、染料木苷、大豆苷元、黄豆苷元、染料木素这六种化合物组成。它们的线性范围分别为0.1~4.1μg/mL、0.11~4.36μg/mL、0.12~4.84μg/mL、0.1~4μg/mL、0.11~4.58μg/mL、0.11~4.37μg/mL。大豆异黄酮原料和产品药片的相对标准偏差(RSD)分别为4%和2%,原料和片剂中各成分的回收率均在90%~110%之间,且所有组分分析完成时间不超过10分钟。大豆异黄酮量的准确测定保证了大豆异黄酮类原料中规定的异黄酮量,以及产品标签上的正确异黄酮量。
文摘The initiation and progression of cancer not only involves genetic abnormalities, but also epigenetic alterations, such as DNA methylation and histone modifications. Epigenetics refers to the heritable changes that do not involve any structural changes in the target gene, i.e., DNA sequence and protein sequence. Thus, these epigenetic aberrations are potentially reversible, allowing the malignant cells to revert to a state with more normal characteristics. The use of epigenetics is emerging as an effective and promising approach to treat cancer. Epigenetic drugs, which target two well- known epigenetic pathways, namely, DNA methyltransferases and histone deacetylases, are already being applied for the cancer treatment. In the current study, an overview regarding the under-standing of epigenetic alterations in the development of cancer and the current state of epigenetic drug discovery is provided.
文摘A convenient and effective procedure was developed for the conversion of alcohol to olefin by tosylation and subsequent β-elimination promoted by silica gel in this study. Treatment of the alcohols with p-toluenesulfonyl chloride in pyridine at 0℃ af- fords tosylates which undergo β-elimination with silica gel in dichloromethane or chloroform at room temperature, yielding olefins with high productivity.
文摘Objective： To investigate the anti-proliferative effect of histone deacetylases-1 （HDAC-1） knockdown in Hela cells. Methods： The HDAC-1 protein was knockdowned using siRNA. The expression of HDAC-1 was detected by Western blotting. Apoptosis was assessed by flow cytometry. The inhibition of cell growth was assesses by MTT assay. Results： HDAC-1 siRNA knockdowned the expression of HDAC-1 protein. HDAC siRNA inhibited the proliferation of Hela cells. HDAC- 1 siRNA induced apoptosis. Conclusion： HDAC-1 siRNA may inhibit the growth of Hela cells by inducing apoptosis.
文摘Lymphomas enconlpass a group of malignancies that originate in the lymph nodes or other lymphoid tissues. Epigenetic modification, especially by histone deacetylase （HDACs）, plays a key role during the occurrence and development of lymphomas. Consequently, HDAC inhibitors （HDACIs）, a class of gene expression-modulating drugs, have emerged as promising mechanism-based agents for the treatment of lymphomas. This review presents the rationale of HDAC inhibition, describes the epigenetic-based mechanisms of action of HDACIs, discusses their clinical efficiency, and summarizes the current and future developments in this field.
基金Supported by the National Key Technology Research and Development Program(No.2006AA10A411)the Agricultural Seed Project of Shandong Province
文摘The sea cucumber, Apostichopusjaponicus, undergoes aestivation to improve survival during periods of high-temperature. During aestivation, the metabolic rate is depressed to reduce the consumption of reserved energy. We evaluated the role of epigenetic modification on global gene silencing during metabolic rate depression in the sea cucumber. We compared the expression of epigenetic modifiers in active and aestivating sea cucumbers. The expression of three genes involved in DNA methylation and chromatin remodeling （DNA （cytosine-5）-methyltransferase l, Methyl-CpG-binding domain protein 2）, and Chromodomain-helicase-DNA-binding protein 5） was significantly higher during aestivation （Days 20 and 40）. Similarly, we observed an increase in the expression of genes involved in histone acetylation （Histone deacetylase 3） and Histone-binding protein RBBP4） during the early （Days 5 and 10） and late phases （Days 20 and 40） of aestivation. There was no change in the expression of KAT2B, a histone acetyltransferase. However, the expression of histone methylation associated modifiers （Histone-arginine methyltransferase CARMER and Histone-lysine N-methyltransferase MLL5） was significantly higher after 5 d in the aestivating group. The results suggest that the expression of epigenetic modifiers involved in DNA methylation, chromatin remodeling, histone acetylation, and histone methylation is upregulated during aestivation. We hypothesize that these changes regulate global gene silencing during aestivation in A. japonicus.
