老化参池刺参腐皮综合征致病原的分离与鉴定

从患病刺参体表病灶组织分离出菌株060330B,其优势度高达90%,人工回接感染试验证实其具有较强的致病性,可导致健康刺参出现与自然患病刺参相同的症状。通过形态学观察、API半自动化鉴定和常规生理生化试验的结果表明,菌株060330B具有假单胞菌属的特征,其表型特征与恶臭假单胞菌相似。对该菌株进行16SrRNA基因序列分析和构建系统发育树,结果显示其与恶臭假单胞菌的亲缘关系最近,相似率达到99.5%。菌株060330B可鉴定为恶臭假单胞菌,并视为养殖刺参腐皮综合征的致病原之一。通过对多起不同来源患病刺参的研究表明,使用年限长、淤泥层厚呈黑色并伴有腥味的养殖池塘在该时期容易导致刺参腐皮综合征的发生。

不同水质调控方式对刺参养殖池沉降颗粒中碳氮磷沉降通量影响的季节变化

在相邻且放养规格与密度及投喂等饲养方式相同的600 m×85 m×1.5 m常规刺参(Apostichopus japonicus)养殖池塘中,分别采用3种水质调控方式:自然纳潮水质调控方式(自然池塘)、进水口底部配置微孔曝气增氧设备(微孔曝气池塘)和配置养水机设备的参池(养水机池塘),探究不同水质调控方式对参池沉降颗粒中碳氮磷沉降通量影响的季节变化。2015年10月—2016年9月期间,每月换水后1 d,在各池底部中轴线的进水口、中部、排水口分别用沉降颗粒收集装置(实用新型专利:CN 203178112 U)收集沉降颗粒物样品,测定总碳、总氮和总磷沉降通量。结果表明,夏季自然池塘水中总碳和总氮沉降通量显著低于另两种参池(P<0.05);秋季养水机池塘显著低于另两种参池(P<0.05);冬季养水机池塘总磷沉降通量显著低于另两种参池,与自然池塘差异显著(P<0.05)。结果表明,选择合适的水质辅助设备有助于改善参池水质。

潮间带养鲍池垒石养参

由于养鲍的效益一度较高,近年来,沿海各地在潮间带建有大量鲍池养鲍,但由于后来养鲍效益下降等多种原因,养鲍池的利用率及效益很低.不少地方的池子几近废弃,导致生产物资、设施的极大浪费.若在潮间带养鲍池堆石养参能够有效改变这种被动局面,实现养鲍养参一举两得.现将操作中的主要技术介绍如下,供参考.

Working Condition Real-Time Monitoring Model of Lithium Ion Batteries Based on Distributed Parameter System and Single Particle Model

Lithium ion batteries are complicated distributed parameter systems that can be described preferably by partial differential equations and a field theory. To reduce the solution difficulty and the calculation amount, if a distributed parameter system is described by ordinary differential equations （ODE） during the analysis and the design of distributed parameter system, the reliability of the system description will be reduced, and the systemic errors will be introduced. Studies on working condition real-time monitoring can improve the security because the rechargeable LIBs are widely used in many electronic systems and electromechanical equipment. Single particle model （SPM） is the simplification of LIB under some approximations, and can estimate the working parameters of a LIB at the faster simulation speed. A LIB modelling algorithm based on PDEs and SPM is proposed to monitor the working condition of LIBs in real time. Although the lithium ion concentration is an unmeasurable distributed parameter in the anode of LIB, the working condition monitoring model can track the real time lithium ion concentration in the anode of LIB, and calculate the residual which is the difference between the ideal data and the measured data. A fault alarm can be triggered when the residual is beyond the preset threshold. A simulation example verifies that the effectiveness and the accuracy of the working condition real-time monitoring model of LIB based on PDEs and SPM.

Online SOC estimation based on modified covariance extended Kalman filter for lithium batteries of electric vehicles

To offset the defect of the traditional state of charge(SOC)estimation algorithm of lithium battery for electric vehicle and considering the complex working conditions of lithium batteries,an online SOC estimation algorithm is proposed by combining the online parameter identification method and the modified covariance extended Kalman filter(MVEKF)algorithm.Based on the parameters identified on line with the multiple forgetting factors recursive least squares methods,the newly-established algorithm recalculates the covariance in the iterative process with the modified estimation and updates the process gain which is used for the next state estimation to decrease errors of the filter.Experiments including constant pulse discharging and the dynamic stress test(DST)demonstrate that compared with the EKF algorithm,the MVEKF algorithm produces fewer estimation errors and can reduce the errors to 5%at most under the complex charging and discharging conditions of batteries.In the charging process under the DST condition,the EKF produces a larger deviation and lacks stability,while the MVEKF algorithm can estimate SOC stably and has a strong robustness.Therefore,the established MVEKF algorithm is suitable for complex and changeable working conditions of batteries for electric vehicles.

Optimization of Stocking Density for the Sea Cucumber, Apostichopus japonicus Selenka, Under Feed-Supplement and Non-Feed-Supplement Regimes in Pond Culture

Optimal stocking densities were investigated for the sea cucumber Apostichopusjaponicus Selenka under feed-supplement and non-feed-supplement regimes in net enclosures for 333 d. Substantial weight loss occurred during the aestivation phase （AE）. Decreased growth rates were also observed during the winter phase （WT）. In contrast, sea cucumbers showed rapid growth during the spring （SP） and autumn （AU） phases. Feeding regimes considerably influenced the growtb performance, i.e., sea cucumbers grew faster under feed-supplement regime than under non-feed-supplement regime （P 〈 0.05）. The average survival rates of sea cucumbers under feed-supplement regime were higher than those under non-feed-supplement regime for both the autumn phase and spring phase, but the differences were only significant for the latter phase （P〈 0.05）. The fitted B-N curves showed that the optimal stocking densities, in terms of net production, were 22.3 ind. m^-2 for feed-supplement regime and 14.1 ind. m^-2 for non-feed-supplement regime.

Seasonal Changes in Food Uptake by the Sea Cucumber Apostichopus japonicus in a Farm Pond: Evidence from C and N Stable Isotopes

This study investigated the seasonal changes in carbon (C) and nitrogen (N) stable isotope values of several typical food sources of Apostichopus japonicus in a farm pond, including particulate organic matter (POM), macroalgae, benthic microalgae and animals such as nematode and copepod. The stable isotope technique was used to quantify relative contributions of various sources to the food uptake by A. japonicus. The results showed that significant changes occurred in the C and N stable isotope values of sea cucumber food sources due to the seasonality of micro-or macroalgae prosperity and the fluctuation of environmental conditions. The sea cucumber A. japonicus exhibited corresponding alterations in feeding strategy in response to the changes in food conditions. Calculation with a stable isotope mixing model showed that macroalgae was the principal food source for A. japonicus throughout the 1-yr investigation, with the relative contribution averaging 28.1% - 63.2%. The relative contributions of other food sources such as copepod and nematode, POM, benthic microalgae to the total food uptake by sea cucumber averaged 22.6% - 39.1%, 6.3% - 22.2%, 2.8% - 6.5%, and 2.8% - 4.2%, respectively. Together these results indicated that the seasonal changes in food sources led to the obvious temporal differences in the relative contribution of various food sources utilized by A. japonicus. Such findings provide the basic scientific information for improving the aquaculture techniques of A. japonicus, particularly for optimizing the food environment of A. japonicus culture in farm ponds.

Simulation of second-order RC equivalent circuit model of lithium battery based on variable resistance and capacitance

With the rise of the electric vehicle industry,as the power source of electric vehicles,lithium battery has become a research hotspot.The state of charge(SOC)estimation and modelling of lithium battery are studied in this paper.The ampere-hour(Ah)integration method based on external characteristics is analyzed,and the open-circuit voltage(OCV)method is studied.The two methods are combined to estimate SOC.Considering the accuracy and complexity of the model,the second-order RC equivalent circuit model of lithium battery is selected.Pulse discharge and exponential fitting of lithium battery are used to obtain corresponding parameters.The simulation is carried out by using fixed resistance capacitance and variable resistance capacitor respectively.The accuracy of variable resistance and capacitance model is 2.9%,which verifies the validity of the proposed model.

Selectivity of escape-hole size in tube traps for white-spotted conger Conger myriaster

Comparative fishing experiments were carried out in 2010 using tube traps with five hole diameters (8, 15, 18, 20 and 22 mm) to establish the size selectivity of escape holes for white-spotted conger. Selectivity and split parameters of the SELECT model were calculated using the estimated-split and equal-spilt model. From likelihood ratio tests and AIC (Akaike's Information Criterion) values, the estimated-split model was selected as the best-fit model. Size selectivity of escape holes in the tube traps was expressed as a logistic curve, similar to mesh selectivity. The 50% selection length of white-spotted conger in the estimated-split model was 28.26, 33.35, 39.31 and 47.30 cm for escape-hole diameters of 15, 18, 20 and 22 mm, respectively. The optimum escape-hole size is discussed with respect to management of the white-spotted conger fishery. The results indicate that tube traps with escape holes of 18 mm in diameter would benefit this fishery.

Identification of dynamic model parameters for lithium-ion batteries used in hybrid electric vehicles

An electrical equivalent circuit model for lithium-ion batteries used for hybrid electric vehicles （HEV） is presented. The model has two RC networks characterizing battery activation and concentration polarization process. The parameters of the model are identified using combined experimental and extended Kalman filter （EKF） recursive methods. The open-circuit voltage and ohmic resistance of the battery are directly measured and calculated from experimental measurements, respectively. The rest of the coupled dynamic parameters, i.e. the RC network parameters, are estimated using the EKF method. Experimental and simulation results are presented to demonstrate the efficacy of the proposed circuit model and parameter identification techniques for simulating battery dynamics.

Design and parametric optimization of thermal management of lithium-ion battery module with reciprocating air-flow

Single cell temperature difference of lithium-ion battery(LIB) module will significantly affect the safety and cycle life of the battery. The reciprocating air-flow module created by a periodic reversal of the air flow was investigated in an effort to mitigate the inherent temperature gradient problem of the conventional battery system with a unidirectional coolant flow with computational fluid dynamics(CFD). Orthogonal experiment and optimization design method based on computational fluid dynamics virtual experiments were developed. A set of optimized design factors for the cooling of reciprocating air flow of LIB thermal management was determined. The simulation experiments show that the reciprocating flow can achieve good heat dissipation, reduce the temperature difference, improve the temperature homogeneity and effectively lower the maximal temperature of the modular battery. The reciprocating flow improves the safety, long-term performance and life span of LIB.

Eco-drainage Pond in Non Irrigated Land for the Conservation of Land and Water Based on Community Participation

There are some bad impacts from a critical land such as flood in rainy season and dryness in dry season. Long dryness could cause shortage of water for human consumption, industry and agriculture. Prevention has been made to avoid the both bad impacts by building hydraulic structures like reservoirs and ponds in order to reduce the peak flow during the rainy season and to store the water for use during the dry season. However, they gradually become full of sediment. This will make them work insufficiently and will cause high maintenance cost. Moreover, wells in agriculture field used as retention also suffer dryness to make crop failure. This study aims to develop a model of pond for conservation of land and water in non irrigated land. Community participation is crucial in order to solve the flood and dryness problems. The community, especially the farmers will participate actively in the implementation and maintenance of the model. There are benefits in using the pond model such as： （1） to control sediment transport to the rivers, reservoir or other hydraulic structures; （2） to reduce flood rate; （3） to increase ground water recharge; （4） to contain water for agriculture and （5） to control the fertility of land. The cost of making a unit of model （sedrainpond） of 1.5 m diameter and 1.5 meter depth is relatively cheap between $10 to $20 （US）, and the maintenance is done by the farmers. When the pond model becomes functional, besides containing water in the rainy season, the water can be used to sustain water for agriculture in the dry season to avoid any crop failure. When the pond becomes dry, the farmers can harvest the suspended load sediment such as clay or silt to spread onto their land to keep its fertility.

Experimental Investigation of Environmental Hydraulic Parameters for Dual Mixed Media Biofilter for Greywater Treatment

Laboratory scale model of DMMBF （dual mixed media biofilter） were designed and installed in AI-Mustansiriya University Environmental Hydraulic Lab. Experiments were conducted using two mixed layers through PVR column--2.2 m height and 300 mm diameter. The first mixed media filter of depth 640mm mixed of sand, rice husk and granular activated carbon. The percentage volume mix is 1：1：1. While the other mixed media of depth 740 mm, consisting of coal, crash porcelinaite, rock and granite with equally percentage volume. Fifty samples were collected during the experiments, which was spread over a period of forty two weeks. The obtained results indicate that when the flow loading raised from 0.15 L/min to 2.7 L/rain, the removal efficiency of BOD decreased 8%-11%, and the removal efficiency of COD deceased 3%-4%, while the removal efficiency of turbidity increased with the decreasing of hydraulic loading. The results showed that the removal efficiency of turbidity is more than 95% at the lower discharge （0.15 L/min）. Therefore, infiltration should be conservatively designed using low loading rates.

Ecological effects of co-culturing sea cucumber Apostichopus japonicus (Selenka) with scallop Chlamys farreri in earthen ponds

Monthly changes in sedimentation and sediment properties were studied for three different culture treatments： sea cucumber monoculture （Mc）, sea cucumber and scallop polyculture （Ps-c） and scallop monoculture （Ms）. Results indicated that the survival rate of sea cucumber was significantly higher in Ps-c cultures than in Mc cultures. Sea cucumber yield was 69.6% higher in Ps-c culture than in Mc culture. No significant differences in body weight and scallop shell length were found between Ps-c and Ms cultures. The mean sedimentation rate of total particulate matter （TPM） was 72.2 g/（m^2.d） in Ps-c cultures, with a maximum of 119.7 g/（mE.d）, which was markedly higher than that of Mc （mean value）. Sedimentation rates of organic matter （OM）, total organic carbon （TOC）, total nitrogen （TN） and total phosphorus （TP） in Ps-c cultures were also significantly higher than those in Mc cultures. TOC and TN contents of sediment increased rapidly in the first 5 months in Ms cultures and remained at a high level. TOC and TN contents in Mc and Ps-c cultures decreased during sea cucumber feeding seasons and increased during sea cucumber dormancy periods （summer and winter）. The study demonstrates that co-culture of sea cucumber and scallop in earthen ponds is an alternative way to alleviate nutrient loads and improve water quality in coastal aquaculture systems. Moreover, it provides the additional benefit of an increased sea cucumber yield.

Impact of Korean Ginseng （Panax Ginseng） on Power Generation of Microbial Fuel Cells

This study reports an increase in power generation of a MFC （microbial fuel cell） by the addition of Korean ginseng （Panax ginseng）. It was noted that the use of ginseng enhances the microbial anaerobic degradation of cellobiose, a disaccharide that was used as a substrate in the anode chamber of the MFC. The power output of the MFC where ginseng was added showed noticeable enhancement compared to the control MFC. The increase slowly ramped at the initial days and became appreciably higher after the 11th day of incubation in an experiment set up for 16 days duration. It is attributed that the ginseng increases the CO2 production by accelerating the fermentation process. Decrease in CH4/CO2 ratio was observed also due to decrease in methane production per digested cellobiose, the proton donor in the current study. Four ring steroid-like structural moiety Ginsenoside of Panax ginseng seemed to play a beneficial role in the electron transfer from ceilobiose to the anode, perhaps by rendering easier electron transfer due to favorable energy level alignments.

Effect of Temperature on the Organic Solar Cells Parameters

The dependence of the organic solar cells parameters, e.g., the shirt circuit current Isc, open circuit voltage Vo,., the fill factor FF and the efficiency eta on temperature is investigated. By expressing the different equations which link the parameters with temperature, it is observed that the short circuit current Isc increases so monotonous with temperature and then saturates to a maximum value before decreasing at high temperatures. The open circuit voltage V∝ decreases linearly with the increasing of the temperature. The fill factor FF and the efficiency eta which are directly related with short circuit current Isc and the open circuit voltage V∝ follow their variations.

First-principles investigation on structural and electrochemical properties of NaCoO_2 for rechargeable Na-ion batteries

NaxCoO2 is a commonly used cathode material for sodium ion batteries because of its easy synthesis, high reversible capacity and good cyclability. The structural and electrochemical properties of NaxCoO2 during sodium ion insertion/extraction process are studied based on first principles calculations. The calculation results of crystal structure parameters and average intercalation voltage are in good agreement with experiment data. Through calculation of the geometric structure and charge transfer in charging and discharging processes of NaxCoO2, it is found that the oxygen atom surrounding Co of the CoO6 octahedral screens the coulomb potential produced by sodium vacancy in NaxCoO2, and the charge is removed from the entire Co-O layer instead of the Co atom adjacent to sodium vacancy when sodium ions are extracted from the Na CoO2 lattice. Thus, during the insertion/extraction of sodium ion from Na CoO2, the CoO6 octahedral structure undergoes small lattice distortion, which makes the local structure quite stable and is beneficial to the cycling stability of the material for the application of sodium ion batteries.