To develop a HPIX-UV-MS method for identifying the constituents in theChinese drug Notoginseng (the root of Panax notoginseng). Methods A Phenomenex Luna C_(18) column(250 mm x 4.6 mm ID, 5 μm) was utilized. Water co...To develop a HPIX-UV-MS method for identifying the constituents in theChinese drug Notoginseng (the root of Panax notoginseng). Methods A Phenomenex Luna C_(18) column(250 mm x 4.6 mm ID, 5 μm) was utilized. Water containing 0.005% formic acid (A) and acetonitrilecontaining 0.005% formic acid (B) were used as gradient eluents. UV spectra were recorded in range195 - 400 nm. Both positive and negative ion ESI modes were used. Results The constituents inNotoginseng were well separated and detected. Fourteen compounds were identified by comparing theirretention time and ESI-MS data with those obtained from the reference compounds. Forty-one compoundswere deduced by data analysis of MS and literature; among them, yesanchinosides-H and -E,chikusetsusaponin-L_5, malonyl-ginsenoside-R_(g_1), the isomers of notoginsenosides-J, -A, -R_1, -G,-R_2, and ginsenoside-Rh_3 were discovered in Notoginseng for the first time. Conclusion Thismethod gives high sensitivity and good separation, and is suitable for identifying the constituentsin Notoginseng. This result is helpful for further phytochemical research on Notoginseng. Based onthis result, further quality control can be studied.展开更多
[Objective] The aim was to study the feasibility of using colchicine to induce polyploidy from the buds of Shandong Salvia miltiorrhiza.[Method] The buds of Shandong Salvia miltiorrhiza were induced by colchicine.[Res...[Objective] The aim was to study the feasibility of using colchicine to induce polyploidy from the buds of Shandong Salvia miltiorrhiza.[Method] The buds of Shandong Salvia miltiorrhiza were induced by colchicine.[Result] When the concentration of colchicine supplemented in culture medium was 12 mg/L,the induction rate of polyploidy was the highest(36.6%),but its survival rate was only 16.7%.When the concentration of colchicine was 8 mg/L,the induction rate of polyploidy was 15.9%,and its survival rate was up to 40.0%.Polyploidy plant of Shandong Salvia miltiorrhiza was strong and had thick,large and dark leaves,and long stomata on the lower epidermis of leaves had small density.[Conclusion] Colchicine could induce buds effectively,and the polyploidy breeding material of Salvia miltiorrhiza was obtained,which laid a foundation for shortening breeding process.展开更多
A novel maglev transportation system was proposed for large travel range ultra precision motion.The system consists of a levitation subsystem and a propulsion subsystem.During the propulsion subsystem driving the movi...A novel maglev transportation system was proposed for large travel range ultra precision motion.The system consists of a levitation subsystem and a propulsion subsystem.During the propulsion subsystem driving the moving platform along the guideway,the levitation subsystem uses six pairs of electromagnets to steadily suspend the moving platform over the guideway.The model of the levitation system,which is a typical nonlinear multi-input multi-output coupling system and has many inner nonlinear coupling characteristics,was deduced.For testifying the model,the levitation mechanism was firstly controlled by proportional-integral-differential(PID) control,and then a lot of input-output data were collected for model parameter identification.The least-square parameter identification method was used.The identification results prove that the model is feasible and suitable for the real system.展开更多
In this article, we propose a novel, multilevel, dynamic factor model, to determine endogenously clustered regions for the investigation of regional clustering and synchronization of provincial business fluctuations i...In this article, we propose a novel, multilevel, dynamic factor model, to determine endogenously clustered regions for the investigation of regional clustering and synchronization of provincial business fluctuations in China. The parameter identification and model estimation was conducted using the Markov Chain Monte Carlo method. We then conducted an empirical study of the provincial business fluctuations in China(31 Chinese provinces are considered except Hong Kong, Macao, and Taiwan due to the data unavailability), which were sampled from January 2000 to December 2015. Our results indicated that these provinces could be clustered into four regions: leading, coincident, lagging, and overshooting. In comparison with traditional geographical divisions, this novel clustering into four regions enabled the regional business cycle synchronization to be more accurately captured. Within the four regional clusters it was possible to identify substantial heterogeneities among regional business cycle fluctuations, especially during the periods of the 2008 financial crisis and the ‘four-trillion economic stimulus plan'.展开更多
Gut microorganisms play an important role in the digestion of their host animals. The purpose of this research was to isolate and assess the enzyme-producing microbes from the Apostichopusjaponicus gut. Thirty-nine st...Gut microorganisms play an important role in the digestion of their host animals. The purpose of this research was to isolate and assess the enzyme-producing microbes from the Apostichopusjaponicus gut. Thirty-nine strains that can produce at least one of the three digestive enzymes (protease, amylase, and cellulase) were qualitatively screened based on their extracellular enzyme-producing abilities. The enzyme-producing strains clustered into eight groups at the genetic similarity level of 100% by analyzing the restriction patterns of 16S rDNA amplified with Mbo L Phylogenetic analysis revealed that 37 strains belonged to the genus Bacillus and two were members of the genus Virgibacillus. Enzyme-producing capability results indicate that the main enzyme-producing microflora in the A.japonicus gut was Bacillus, which can produce protease, amylase, and cellulase. Virgibacillus, however, can only produce protease. The high enzyme-producing capability of the isolates suggests that the gut microbiota play an important role in the sea cucumber digestive process.展开更多
A new reaction system to determine nonlinear chemical fingerprint(NCF)and its use in identification method based on double reaction system was researched.Panax ginsengs,such as ginseng,American ginseng and notoginseng...A new reaction system to determine nonlinear chemical fingerprint(NCF)and its use in identification method based on double reaction system was researched.Panax ginsengs,such as ginseng,American ginseng and notoginseng were identified by the method.The NCFs of the three samples of Panax ginsengs were determined through two nonlinear chemical systems,namely system 1 consisting of sample components,H2SO4,MnSO4,NaBrO3,acetone and the new system,system 2 consisting of sample components,H2SO4,(NH4)4Ce(SO4)2,NaBrO3 and citric acid.The comparison between the results determined through systems 1 and 2 shows that the speed to determine NCF through system 2 is much faster than that through system 1;for systems 1 and 2,the system similarities of the same kind of samples are≥98.09%and 99.78%,respectively,while those of different kinds of samples are≤63.04%and 86.34%,respectively.The results to identify the kinds of some samples by system similarity pattern show that both the accuracies of identification methods based on single system 1 and 2 are≥95.6%,and the average values are 97.1%and 96.3%,respectively;the accuracy of the method based on double system is≥97.8%,and the average accuracy is 99.3%.The accuracy of the method based on double system is higher than that based on any single system.展开更多
A model to describe the hysteresis damper character of rubber material is presented in this paper. It consists of a parallel spring and damper, whose coefficients change with vibration frequencies. In order to acquire...A model to describe the hysteresis damper character of rubber material is presented in this paper. It consists of a parallel spring and damper, whose coefficients change with vibration frequencies. In order to acquire these relations, the force decomposition is carried out according to some sine vibration measurement data about nonlinear forces changing with deformations of the rubber material. The nonlinear force is decomposed into a spring force and a damper force, which are represented by a frcquency-dependent spring and damper coefficient, respectively. Repeating this step for different measurements will give different coefficients corresponding to different frequencies. Then, application of a parameter identification method will provide the requested functions over frequency. Using those formulae, as an example, the dynamic character of a hollow shaft system supported by rubber rings is analyzed and the acceleration response curve in the centroid position is calculated. Comparisons with sine vibration experiments of the real system show a maximal inaccuracy of 8. 8 %. Application of this model and procedure can simplify the modeling and analysis of mechanical systems including rubber materials.展开更多
Sea cucumbers are traditional marine food and Chinese medicine in Asia. The rapid expansion of sea cucumber market has resulted in various problems, such as commercial fraud and mislabeling. Conventionally, sea cucumb...Sea cucumbers are traditional marine food and Chinese medicine in Asia. The rapid expansion of sea cucumber market has resulted in various problems, such as commercial fraud and mislabeling. Conventionally, sea cucumber species could be distinguished by their morphological and anatomical characteristics; however, their identification becomes difficult when they are processed. The aim of this study was to develop a new convenient method of identifying and distinguishing sea cucumber species. Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) analysis of mitochondrial cytochrome oxidase I gene(COI) was used to identifing five sea cucumber species(Apostichopus japonicus, Cucumaria frondosa, Thelenota ananas, Parastichopus californicus and Actinopyga lecanora). A 692 bp fragment of COI was searched for BamHI, KpnI, PstI, XbaI and Eco31 I restriction sites with DNAMAN 6.0, which were then used to PCR-RFLP analysis. These five sea cucumber species can be discriminated from mixed sea cucumbers. The developed PCR-RFLP assay will facilitate the identification of sea cucumbers, making their source tracing and quality controlling feasible.展开更多
In this study,the fatty acids(FAs) of the organs and tissues of sea cucumber(Apostichopus japonicus) were profiled in order to compare the FA composition of sea cucumber collected from natural habitat(wild) and cages(...In this study,the fatty acids(FAs) of the organs and tissues of sea cucumber(Apostichopus japonicus) were profiled in order to compare the FA composition of sea cucumber collected from natural habitat(wild) and cages(cultured).The differences in FA contents in dermomuscular tube,peripharyngeal annulus,gonad and intestine(with or without content) between the wild and the cultured were determined.The main fatty acids in all organs and tissues were 20:5n-3,16:1n-7,20:4n-6,22:6n-3,18:0,and 18:1n-7.The basically different FAs of body wall and digestive tube were 16:1n-7,18:1n-9 and 20:1n-11.The ratio of saturated to mono-and polyunsaturated FAs in digestive tube was independent on inside content while there was a redistribution of the total amount of n-3 and n-6 fatty acids.The comparison of FA composition of the wild and the cultured sea cucumber showed that 20:5n-3,16:1n-7 and 18:1n-7 predominated the wild while 20:4n-6 predominated the cultured.The content of branched-chain fatty acids in the wild was 3%–4% and about 9% in the cultured.The possible FAs for identifying the wild and the cultured sea cucumbers were selected.It was suggested that the indexes such as the ratio of either(n-3:n-6) to(n-7:n-6) or(n-3) +(n-7) to(n-6) may serve as the biomarkers distinguishing the wild and the cultured sea cucumber.展开更多
The paper is devoted to the elastostatic calibration of industrial robots, which is used for precise machining of large-dimensional parts made of composite materials. In this technological process, the interaction bet...The paper is devoted to the elastostatic calibration of industrial robots, which is used for precise machining of large-dimensional parts made of composite materials. In this technological process, the interaction between the robot and the workpiece causes essential elastic deflections of the manipulator components that should be compensated by the robot controller using relevant elastostatic model of this mechanism. To estimate parameters of this model, an advanced calibration technique is applied that is based on the non-linear experiment design theory, which is adopted for this particular application. In contrast to previous works, it is proposed a concept of the user-defined test-pose, which is used to evaluate the calibration experiments quality. In the frame of this concept, the related optimization problem is defined and numerical routines are developed, which allow generating optimal set of manipulator configurations and corresponding forces/torques for a given number of the calibration experiments. Some specific kinematic constraints are also taken into account, which insure feasibility of calibration experiments for the obtained configurations and allow avoiding collision between the robotic manipulator and the measurement equipment. The efficiency of the developed technique is illustrated by an application example that deals with elastostatic calibration of the serial manipulator used for robot-based machining.展开更多
The sea cucumber Apostichopus japonicus is a commercially and ecologically important species in China. A total of 3056 potential unigenes were generated after assembling 7597 A. japonicus expressed sequence tags (ESTs...The sea cucumber Apostichopus japonicus is a commercially and ecologically important species in China. A total of 3056 potential unigenes were generated after assembling 7597 A. japonicus expressed sequence tags (ESTs) downloaded from Gen-Bank. Two hundred and fifty microsatellite-containing ESTs (8.18%) and 299 simple sequence repeats (SSRs) were detected. The average density of SSRs was 1 per 7.403 kb of EST after redundancy elimination. Di-nucleotide repeat motifs appeared to be the most abundant type with a percentage of 69.90%. Of the 126 primer pairs designed, 90 amplified the expected products and 43 showed polymorphism in 30 individuals tested. The number of alleles per locus ranged from 2 to 26 with an average of 7.0 alleles, and the observed and expected heterozygosities varied from 0.067 to 1.000 and from 0.066 to 0.959, respectively. These new EST-derived microsatellite markers would provide sufficient polymorphism for population genetic studies and genome mapping of this sea cucumber species.展开更多
基金NationalBasicResearchProgramofChina (No .G19990 5 44 0 6)NationalNaturalScienceFoundationofChina(No .3 9970 898)
文摘To develop a HPIX-UV-MS method for identifying the constituents in theChinese drug Notoginseng (the root of Panax notoginseng). Methods A Phenomenex Luna C_(18) column(250 mm x 4.6 mm ID, 5 μm) was utilized. Water containing 0.005% formic acid (A) and acetonitrilecontaining 0.005% formic acid (B) were used as gradient eluents. UV spectra were recorded in range195 - 400 nm. Both positive and negative ion ESI modes were used. Results The constituents inNotoginseng were well separated and detected. Fourteen compounds were identified by comparing theirretention time and ESI-MS data with those obtained from the reference compounds. Forty-one compoundswere deduced by data analysis of MS and literature; among them, yesanchinosides-H and -E,chikusetsusaponin-L_5, malonyl-ginsenoside-R_(g_1), the isomers of notoginsenosides-J, -A, -R_1, -G,-R_2, and ginsenoside-Rh_3 were discovered in Notoginseng for the first time. Conclusion Thismethod gives high sensitivity and good separation, and is suitable for identifying the constituentsin Notoginseng. This result is helpful for further phytochemical research on Notoginseng. Based onthis result, further quality control can be studied.
基金Supported by Industrial Scientific Special Project of Ministry of Agriculture(200803034)Postdoctoral Innovation Project of Shandong Province(200903017)~~
文摘[Objective] The aim was to study the feasibility of using colchicine to induce polyploidy from the buds of Shandong Salvia miltiorrhiza.[Method] The buds of Shandong Salvia miltiorrhiza were induced by colchicine.[Result] When the concentration of colchicine supplemented in culture medium was 12 mg/L,the induction rate of polyploidy was the highest(36.6%),but its survival rate was only 16.7%.When the concentration of colchicine was 8 mg/L,the induction rate of polyploidy was 15.9%,and its survival rate was up to 40.0%.Polyploidy plant of Shandong Salvia miltiorrhiza was strong and had thick,large and dark leaves,and long stomata on the lower epidermis of leaves had small density.[Conclusion] Colchicine could induce buds effectively,and the polyploidy breeding material of Salvia miltiorrhiza was obtained,which laid a foundation for shortening breeding process.
基金Projects(50735007,51005253) supported by the National Natural Science Foundation of ChinaProject(2007AA04Z344) supported by the National High-Tech Research and Development Program of China
文摘A novel maglev transportation system was proposed for large travel range ultra precision motion.The system consists of a levitation subsystem and a propulsion subsystem.During the propulsion subsystem driving the moving platform along the guideway,the levitation subsystem uses six pairs of electromagnets to steadily suspend the moving platform over the guideway.The model of the levitation system,which is a typical nonlinear multi-input multi-output coupling system and has many inner nonlinear coupling characteristics,was deduced.For testifying the model,the levitation mechanism was firstly controlled by proportional-integral-differential(PID) control,and then a lot of input-output data were collected for model parameter identification.The least-square parameter identification method was used.The identification results prove that the model is feasible and suitable for the real system.
基金Under the auspices of the National Natural Science Foundation of China(No.71371160)the Program for Changjiang Youth Scholars(No.Q2016131)the Program for New Century Excellent Talents in University(No.NCET-13-0509)
文摘In this article, we propose a novel, multilevel, dynamic factor model, to determine endogenously clustered regions for the investigation of regional clustering and synchronization of provincial business fluctuations in China. The parameter identification and model estimation was conducted using the Markov Chain Monte Carlo method. We then conducted an empirical study of the provincial business fluctuations in China(31 Chinese provinces are considered except Hong Kong, Macao, and Taiwan due to the data unavailability), which were sampled from January 2000 to December 2015. Our results indicated that these provinces could be clustered into four regions: leading, coincident, lagging, and overshooting. In comparison with traditional geographical divisions, this novel clustering into four regions enabled the regional business cycle synchronization to be more accurately captured. Within the four regional clusters it was possible to identify substantial heterogeneities among regional business cycle fluctuations, especially during the periods of the 2008 financial crisis and the ‘four-trillion economic stimulus plan'.
基金Supported by the National High Technology Research and Development Program of China(863 Program)(No.2012AA10A412)the National Natural Science Foundation of China(No.41106145)+2 种基金the National Key Technology R&D Program of China(No.2011BAD13B02)the Science and Technology Development Planning Project of Shandong Province(No.2012GGA06021)the Key Laboratory of Mariculture&Stock Enhancement in North China’s Sea,Ministry of Agriculture,China(No.2014-MSENC-KF-03)
文摘Gut microorganisms play an important role in the digestion of their host animals. The purpose of this research was to isolate and assess the enzyme-producing microbes from the Apostichopusjaponicus gut. Thirty-nine strains that can produce at least one of the three digestive enzymes (protease, amylase, and cellulase) were qualitatively screened based on their extracellular enzyme-producing abilities. The enzyme-producing strains clustered into eight groups at the genetic similarity level of 100% by analyzing the restriction patterns of 16S rDNA amplified with Mbo L Phylogenetic analysis revealed that 37 strains belonged to the genus Bacillus and two were members of the genus Virgibacillus. Enzyme-producing capability results indicate that the main enzyme-producing microflora in the A.japonicus gut was Bacillus, which can produce protease, amylase, and cellulase. Virgibacillus, however, can only produce protease. The high enzyme-producing capability of the isolates suggests that the gut microbiota play an important role in the sea cucumber digestive process.
基金Project(61533021)supported by the National Natural Science Foundation of ChinaProject(R201706)supported by Hunan Food Pharmaceutical,China
文摘A new reaction system to determine nonlinear chemical fingerprint(NCF)and its use in identification method based on double reaction system was researched.Panax ginsengs,such as ginseng,American ginseng and notoginseng were identified by the method.The NCFs of the three samples of Panax ginsengs were determined through two nonlinear chemical systems,namely system 1 consisting of sample components,H2SO4,MnSO4,NaBrO3,acetone and the new system,system 2 consisting of sample components,H2SO4,(NH4)4Ce(SO4)2,NaBrO3 and citric acid.The comparison between the results determined through systems 1 and 2 shows that the speed to determine NCF through system 2 is much faster than that through system 1;for systems 1 and 2,the system similarities of the same kind of samples are≥98.09%and 99.78%,respectively,while those of different kinds of samples are≤63.04%and 86.34%,respectively.The results to identify the kinds of some samples by system similarity pattern show that both the accuracies of identification methods based on single system 1 and 2 are≥95.6%,and the average values are 97.1%and 96.3%,respectively;the accuracy of the method based on double system is≥97.8%,and the average accuracy is 99.3%.The accuracy of the method based on double system is higher than that based on any single system.
文摘A model to describe the hysteresis damper character of rubber material is presented in this paper. It consists of a parallel spring and damper, whose coefficients change with vibration frequencies. In order to acquire these relations, the force decomposition is carried out according to some sine vibration measurement data about nonlinear forces changing with deformations of the rubber material. The nonlinear force is decomposed into a spring force and a damper force, which are represented by a frcquency-dependent spring and damper coefficient, respectively. Repeating this step for different measurements will give different coefficients corresponding to different frequencies. Then, application of a parameter identification method will provide the requested functions over frequency. Using those formulae, as an example, the dynamic character of a hollow shaft system supported by rubber rings is analyzed and the acceleration response curve in the centroid position is calculated. Comparisons with sine vibration experiments of the real system show a maximal inaccuracy of 8. 8 %. Application of this model and procedure can simplify the modeling and analysis of mechanical systems including rubber materials.
基金supported by National Natural Science Foundation of China (Nos. 31101281 and 31071525)National Marine Public Welfare Scientific Research Project of China (No. 201105029)
文摘Sea cucumbers are traditional marine food and Chinese medicine in Asia. The rapid expansion of sea cucumber market has resulted in various problems, such as commercial fraud and mislabeling. Conventionally, sea cucumber species could be distinguished by their morphological and anatomical characteristics; however, their identification becomes difficult when they are processed. The aim of this study was to develop a new convenient method of identifying and distinguishing sea cucumber species. Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) analysis of mitochondrial cytochrome oxidase I gene(COI) was used to identifing five sea cucumber species(Apostichopus japonicus, Cucumaria frondosa, Thelenota ananas, Parastichopus californicus and Actinopyga lecanora). A 692 bp fragment of COI was searched for BamHI, KpnI, PstI, XbaI and Eco31 I restriction sites with DNAMAN 6.0, which were then used to PCR-RFLP analysis. These five sea cucumber species can be discriminated from mixed sea cucumbers. The developed PCR-RFLP assay will facilitate the identification of sea cucumbers, making their source tracing and quality controlling feasible.
文摘In this study,the fatty acids(FAs) of the organs and tissues of sea cucumber(Apostichopus japonicus) were profiled in order to compare the FA composition of sea cucumber collected from natural habitat(wild) and cages(cultured).The differences in FA contents in dermomuscular tube,peripharyngeal annulus,gonad and intestine(with or without content) between the wild and the cultured were determined.The main fatty acids in all organs and tissues were 20:5n-3,16:1n-7,20:4n-6,22:6n-3,18:0,and 18:1n-7.The basically different FAs of body wall and digestive tube were 16:1n-7,18:1n-9 and 20:1n-11.The ratio of saturated to mono-and polyunsaturated FAs in digestive tube was independent on inside content while there was a redistribution of the total amount of n-3 and n-6 fatty acids.The comparison of FA composition of the wild and the cultured sea cucumber showed that 20:5n-3,16:1n-7 and 18:1n-7 predominated the wild while 20:4n-6 predominated the cultured.The content of branched-chain fatty acids in the wild was 3%–4% and about 9% in the cultured.The possible FAs for identifying the wild and the cultured sea cucumbers were selected.It was suggested that the indexes such as the ratio of either(n-3:n-6) to(n-7:n-6) or(n-3) +(n-7) to(n-6) may serve as the biomarkers distinguishing the wild and the cultured sea cucumber.
文摘The paper is devoted to the elastostatic calibration of industrial robots, which is used for precise machining of large-dimensional parts made of composite materials. In this technological process, the interaction between the robot and the workpiece causes essential elastic deflections of the manipulator components that should be compensated by the robot controller using relevant elastostatic model of this mechanism. To estimate parameters of this model, an advanced calibration technique is applied that is based on the non-linear experiment design theory, which is adopted for this particular application. In contrast to previous works, it is proposed a concept of the user-defined test-pose, which is used to evaluate the calibration experiments quality. In the frame of this concept, the related optimization problem is defined and numerical routines are developed, which allow generating optimal set of manipulator configurations and corresponding forces/torques for a given number of the calibration experiments. Some specific kinematic constraints are also taken into account, which insure feasibility of calibration experiments for the obtained configurations and allow avoiding collision between the robotic manipulator and the measurement equipment. The efficiency of the developed technique is illustrated by an application example that deals with elastostatic calibration of the serial manipulator used for robot-based machining.
基金supported by the grants from the Science and Technology Development Program of Shandong Province (2009GG10005013)Doctoral Program of Ministry of Education of China (20090132110018)
文摘The sea cucumber Apostichopus japonicus is a commercially and ecologically important species in China. A total of 3056 potential unigenes were generated after assembling 7597 A. japonicus expressed sequence tags (ESTs) downloaded from Gen-Bank. Two hundred and fifty microsatellite-containing ESTs (8.18%) and 299 simple sequence repeats (SSRs) were detected. The average density of SSRs was 1 per 7.403 kb of EST after redundancy elimination. Di-nucleotide repeat motifs appeared to be the most abundant type with a percentage of 69.90%. Of the 126 primer pairs designed, 90 amplified the expected products and 43 showed polymorphism in 30 individuals tested. The number of alleles per locus ranged from 2 to 26 with an average of 7.0 alleles, and the observed and expected heterozygosities varied from 0.067 to 1.000 and from 0.066 to 0.959, respectively. These new EST-derived microsatellite markers would provide sufficient polymorphism for population genetic studies and genome mapping of this sea cucumber species.