期刊文献+
共找到5篇文章
< 1 >
每页显示 20 50 100
基于TRIZ理论双人脚踏车的创新设计 被引量:2
1
作者 张东生 张璐 +1 位作者 孙伟 闫坤 《机械工程师》 2014年第8期50-52,共3页
通过对传统双人脚踏车传动机构和整体结构的研究和分析,找出了存在的问题,建立了问题模型。根据TRIZ理论中的矛盾矩阵表,选择合适的发明创新原理,并利用原理类比移植的思想,对双人脚踏车进行创新设计,设计出一种具有速度合成功能,休闲... 通过对传统双人脚踏车传动机构和整体结构的研究和分析,找出了存在的问题,建立了问题模型。根据TRIZ理论中的矛盾矩阵表,选择合适的发明创新原理,并利用原理类比移植的思想,对双人脚踏车进行创新设计,设计出一种具有速度合成功能,休闲性更高的新型双人脚踏车,结果表明TRIZ理论在机械创新设计和优化设计的可行性和高效性。 展开更多
关键词 双人脚踏车 TRIZ理论 矛盾冲突 发明原理
下载PDF
liver and the defects of cholesterol and bile acids biosynthesis: rare disorders many diagnostic pitfalls 被引量:2
2
作者 Gaetano Corso Antonio Dello Russo Monica Gelzo 《World Journal of Gastroenterology》 SCIE CAS 2017年第29期5257-5265,共9页
In recent decades, biotechnology produced a growth of knowledge on the causes and mechanisms of metabolic diseases that have formed the basis for their study, diagnosis and treatment. Unfortunately, it is well known t... In recent decades, biotechnology produced a growth of knowledge on the causes and mechanisms of metabolic diseases that have formed the basis for their study, diagnosis and treatment. Unfortunately, it is well known that the clinical features of metabolic diseases can manifest themselves with very different characteristics and escape early detection. Also, it is well known that the prognosis of many metabolic diseases is excellent if diagnosed and treated early. In this editorial we briefly summarized two groups of inherited metabolic diseases, the defects of cholesterol biosynthesis and those of bile acids. Both groups show variable clinical manifestations but some clinical signs and symptoms are common in both the defects of cholesterol and bile acids. The differential diagnosis can be made analyzing sterol profiles in blood and/or bile acids in blood and urine by chromatographic techniques(GC-MS and LC-MS/MS). Several defects of both biosynthetic pathways are treatable so early diagnosis is crucial. Unfortunately their diagnosis is made too late, due either to the clinical heterogeneity of the syndromes(severe, mild and very mild) that to the scarcity of scientific dissemination of these rare diseases. Therefore, the delay in diagnosis leads the patient to the medical observation when the disease has produced irreversible damages to the body. Here, we highlighted simple clinical and laboratory descriptions that can potentially make you to suspect a defect in cholesterol biosynthesis and/or bile acids, as well, we suggest appropriate request of the laboratory tests that along with common clinical features can help to diagnose these defects. 展开更多
关键词 CHOLESTEROL Bile acids Liver metabolism Gas chromatography coupled to mass spectrometry Liquid chromatography coupled to tandem mass spectrometry
下载PDF
Evaluation of the hepatoprotective and antioxidant activities of Rubus parvifolius L. 被引量:7
3
作者 Jie GAO Cui-rong SUN +5 位作者 Jie-hong YANG Jian-mei SHI Yue-guang DU Yu-yan ZHANG Jin-hui LI Hai-tong WAN 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2011年第2期135-142,共8页
The hepatoprotective and antioxidant activities of the n-butanol extract of Rubus parvifolius L. (RPL), a widely used medicinal plant, were evaluated. Results demonstrated that RPL extract possessed pronounced hepat... The hepatoprotective and antioxidant activities of the n-butanol extract of Rubus parvifolius L. (RPL), a widely used medicinal plant, were evaluated. Results demonstrated that RPL extract possessed pronounced hepatoprotective effects against carbon tetrachloride (CCI4)-induced hepatic injury in mice, which was at least partially attributed to its strong antioxidant capacity. Treatment with RPL extract markedly attenuated the increases in serum alanine ami- notransferase (ALT) and aspartate aminotransferase (AST) levels caused by CCI4 intoxication. It also significantly prevented the decrease in superoxide dismutase (SOD) activity and the increase in malondialdehyde (MDA) content of liver tissue. Meanwhile, histopathological changes of hepatic damage were also remarkably ameliorated. Phytochemical analysis based on high-performance liquid chromatography/tandem mass spectrometry (HPLC-MS/MS) revealed the presence of various phenolic compounds, including caffeic acid conjugates, ellagic acid glycosides, and flavonol glycosides, which might be responsible for the hepatoprotective and antioxidant activities of RPL. 展开更多
关键词 Rubus parvifofius L. High-performance liquid chromatography/tandem mass spectrometry (HPLC-MS/MS) Hepatoprotective activity Antioxidant activity
原文传递
FLT3 and NPM1 mutations in Chinese patients with acute myeloid leukemia and normal cytogenetics 被引量:4
4
作者 Lei WANG Wei-lai XU +10 位作者 Hai-tao MENG Wen-bin QIAN Wen-yuan MAI Hong-yan TONG Li-ping MAO Yin TONG Jie-jing QIAN Yin-jun LOU Zhi-mei CHEN Yun-gui WANG Jie JIN 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2010年第10期762-770,共9页
Mutations of fins-like tyrosine kinase 3 (FLT3) and nucleophosmin (NPM1) exon 12 genes are the most common abnormalities in adult acute myeloid leukemia (AML) with normal cytogenetics. To assess the prognostic i... Mutations of fins-like tyrosine kinase 3 (FLT3) and nucleophosmin (NPM1) exon 12 genes are the most common abnormalities in adult acute myeloid leukemia (AML) with normal cytogenetics. To assess the prognostic impact of the two gene mutations in Chinese AML patients, we used multiplex polymerase chain reaction (PCR) and capillary electrophoresis to screen 76 AML patients with normal cytogenetics for mutations in FLT3 internal tandem duplication (FLT3/ITD) and exon 12 of the NPM1 gene. FLT3/ITD mutation was detected in 15 (19.7%) of 76 subjects, and NPM1 mutation in 20 (26.3%) subjects. Seven (9.2%) cases were positive for both FLT3/ITD and NPM1 mutations Significantly more FLT3/ITD aberration was detected in subjects with French-American-British (FAB) M1 (42.8%). NPM1 mutation was frequently detected in subjects with M5 (47.1%) and infrequently in subjects with M2 (11.1%). FLT3 and NPM1 mutations were significantly associated with a higher white blood cell count in peripheral blood and a lower CD34 antigen expression, but not age, sex, or platelet count. Statistical analysis revealed that the FLT3/ITD- positive group had a lower complete remission (CR) rate (53.3% vs. 83.6%). Survival analysis showed that the FLT3/ITD-positive/NPM1 mutation-negative group had worse overall survival (OS) and relapse-free survival (RFS). The FLT3/ITD-positive/NPM1 mutation-positive group showed a trend towards favorable survival compared with the FLT3/ITD-positive/NPM1 mutation-negative group (P=0.069). Our results indicate that the FLT3/ITD mutation might be a prognostic factor for an unfavorable outcome in Chinese AML subjects with normal cytogenetics, while NPM1 mutation may be a favorable prognostic factor for OS and RFS in the presence of FLT3/ITD. 展开更多
关键词 Acute myeloid leukemia (AML) Normal cytogenetics Prognosis fms-like tyrosine kinase 3 interna tandem duplication (FLT3/ITD) Nucleophosmin (NPM1) Mutation
原文传递
Analysis of 19-nortestosterone residue in animal tissues by ion-trap gas chromatography-tandem mass spectrometry
5
作者 Jin-qing JIANG Lei ZHANG +6 位作者 Guang-ling LI Hai-tang ZHANG Xue-feng YANG Jun-wei LIU Ren-feng LI Zi-liang WANG Jian-hua WANG 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2011年第6期460-467,共8页
A rapid sample treatment procedure for the gas chromatography-tandem mass spectrometry (GC-MS) determination of 19-nortestosterone (19-NT) in animal tissues has been developed. In our optimized procedures, enzymat... A rapid sample treatment procedure for the gas chromatography-tandem mass spectrometry (GC-MS) determination of 19-nortestosterone (19-NT) in animal tissues has been developed. In our optimized procedures, enzymatic hydrolysis with β-glucuronidase from Escherichia coliwas performed in an acetate buffer (pH 5.2, 0.2 mol/L) Next, the homogenate was mixed with methanol and heated at 60℃ for 15 min, then placed in an ice-bath at -18℃ for 2 h. After liquid-liquid extraction with n-hexane, the analytes were subjected to a normal-phase solid phase ex- traction (SPE) C18 cartridge for clean-up. The dded organic extracts were derivatized with heptafluorobutydc anhydride (HFBA), and then the products were injected into GC-MS. Using electron impact mass spectrometry (El-MS) with positive chemical ionization (PCI), four diagnostic ions (mlz 666, 453, 318, and 306) were determined. A standard calibration curve over the concentration range of 1-20 ng/g was reached, with Y=467084X-68354 (R^2=0.9997) for 19-NT, and the detection limit was 0.3 ng. When applied to spiked samples collected from bovine and ovine, the recoveries ranged from 63% to 101% with relative standard deviation (RSD) between 2.7% and 8.9%. The procedure is a highly efficient, sensitive, and more economical method which offers considerable potential to resolve cases of suspected nandrolone doping in husbandry animals. 展开更多
关键词 19-Nortestosterone (19-NT) Gas chromatography-tandem mass spectrometry (GC-MS) Animal tissues
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部