现有的硬质红粒春小麦(Triticum aestivum L. em Thell.)品种对于由病原菌Cochliobolus sativus (Ito和Kurib) Dreschl.ex Das ur引起的根腐病仅表现中抗水平.本研究的目的是鉴定来自方穗山羊草(节节麦)和冰草属几个种的小麦异源双倍体...现有的硬质红粒春小麦(Triticum aestivum L. em Thell.)品种对于由病原菌Cochliobolus sativus (Ito和Kurib) Dreschl.ex Das ur引起的根腐病仅表现中抗水平.本研究的目的是鉴定来自方穗山羊草(节节麦)和冰草属几个种的小麦异源双倍体和染色体代换系是否能作为根腐病的新抗原.建立了携带根腐病抗性的Agrotana即小麦-茸毛冰草双倍体的温室研究系列.对由方穗山羊草第1-7条染色体代换冬小麦品种Winalta D组染色体的代换系的试验表明,它们对根腐病严重程度的影响不稳定.同样,长穗冰草部分同源染色体代换Winalta 4D、5D和6D染色体的代换系,以及中间冰草的第4条染色体代换Winalta 4B染色体的代换系,对根腐病严重程度没有影响.地下根颈节间的分离培养表明,用C.sativus侵染Agrotana,植株发病率明显比其他外来品系和普通小麦品种都低,这表明Agrotana可能对根腐病具有特异的抗性机制.展开更多
Genomic triplication of the α synuclein gene (SNCA) has been reported to cau se hereditary early onset parkinsonism with dementia. These findings prompted u s to screen for multiplication of the SNCA locus in nine fa...Genomic triplication of the α synuclein gene (SNCA) has been reported to cau se hereditary early onset parkinsonism with dementia. These findings prompted u s to screen for multiplication of the SNCA locus in nine families in whom parkin sonism segregates as an autosomal dominant trait. One kindred was identified wit h SNCA duplication by semiquantitative PCR and confirmed by fluorescent in situ hybridisation analysis in peripheral leucocytes. By contrast with SNCA triplica tion families, the clinical phenotype of SNCA duplication closely resembles idio pathic Parkinsons disease, which has a late age of onset, progresses slowly, and in which neither cognitive decline nor dementia are prominent. These findin gs suggest a direct relation between SNCA gene dosage and disease progression.展开更多
The α synuclein gene (SNCA) has been implicated in autosomal dominant forms of Parkinsons disease. We screened 119 individuals from families with this rar e form of the disease for SNCA duplications by semiquantita...The α synuclein gene (SNCA) has been implicated in autosomal dominant forms of Parkinsons disease. We screened 119 individuals from families with this rar e form of the disease for SNCA duplications by semiquantitative multiplex PCR. T wo patients had duplications, which were confirmed by analysis of intragenic and flanking microsatellite markers. The phenotype in both patients was indistingui shable from idiopathic Parkinsons disease and no atypical features were presen t, by contrast with reports of families with triplication of the same gene. Thes e results indicate that SNCA is more frequently associated with familial Parkins ons disease than previously thought, and that there is a clear dosage effect a ccording to the number of supernumerary copies of this gene.展开更多
Background and aim: The importance of the hepatocyte ploidisation pattern to the control of cell proliferation and differentiation has been well established. However, there are no data that have characterised hepatocy...Background and aim: The importance of the hepatocyte ploidisation pattern to the control of cell proliferation and differentiation has been well established. However, there are no data that have characterised hepatocyte ploidy at various stages of chronic liver inflammation and fibrosis in vivo. Methods: We therefore investigated hepatocyte ploidy/binuclearity patterns in 57 patients with chronic hepatitis, using a recently developed methodology which allows simultaneous hepatocyte ploidy and binuclearity analyses on the same liver section. Results: The percentage of mononuclear diploid hepatocytes was significantly reduced in patients with high hepatitis activity and marked fibrosis (low activity: 75.1 (18.8)%v high activity: 61.8 (21.6)%, p = 0.0111, and low fibrosis: 77.3 (13.8)%v high fibrosis: 57.4 (23.3)%, p = 0.0002). Accordingly, the percentage of mononuclear polyploid hepatocytes increased in patients with high hepatitis activity and marked fibrosis (low activity: 11.9 (15.5)%v high activity: 22.2 (20.1)%, p = 0.0166, and low fibrosis: 9.4 (10.7)%v high fibrosis: 26.4 (21.6)%, p = 0.0001). In addition, the fraction of binuclear hepatocytes was significantly higher in patients with hepatitis B virus (HBV) than in those with hepatitis C virus (HCV) infections (HBV: 18.2 (7.6)%v HCV: 12.0 (4.8)%; p = 0.0020). Under multivariate analysis, HBV infection was an independent factor accounting for the larger binuclear hepatocyte fraction (p = 0.0294). Conclusion: Our results revealed an increase in the polyploid hepatocyte fraction which correlates with the severity of chronic hepatitis; moreover, we demonstrated that HBV and HCV related chronic hepatitis exhibited distinctive hepatocyte ploidy patterns, thus allowing the suggestion that these two viral infections may modulate liver ploidy through different mechanisms.展开更多
文摘现有的硬质红粒春小麦(Triticum aestivum L. em Thell.)品种对于由病原菌Cochliobolus sativus (Ito和Kurib) Dreschl.ex Das ur引起的根腐病仅表现中抗水平.本研究的目的是鉴定来自方穗山羊草(节节麦)和冰草属几个种的小麦异源双倍体和染色体代换系是否能作为根腐病的新抗原.建立了携带根腐病抗性的Agrotana即小麦-茸毛冰草双倍体的温室研究系列.对由方穗山羊草第1-7条染色体代换冬小麦品种Winalta D组染色体的代换系的试验表明,它们对根腐病严重程度的影响不稳定.同样,长穗冰草部分同源染色体代换Winalta 4D、5D和6D染色体的代换系,以及中间冰草的第4条染色体代换Winalta 4B染色体的代换系,对根腐病严重程度没有影响.地下根颈节间的分离培养表明,用C.sativus侵染Agrotana,植株发病率明显比其他外来品系和普通小麦品种都低,这表明Agrotana可能对根腐病具有特异的抗性机制.
文摘Genomic triplication of the α synuclein gene (SNCA) has been reported to cau se hereditary early onset parkinsonism with dementia. These findings prompted u s to screen for multiplication of the SNCA locus in nine families in whom parkin sonism segregates as an autosomal dominant trait. One kindred was identified wit h SNCA duplication by semiquantitative PCR and confirmed by fluorescent in situ hybridisation analysis in peripheral leucocytes. By contrast with SNCA triplica tion families, the clinical phenotype of SNCA duplication closely resembles idio pathic Parkinsons disease, which has a late age of onset, progresses slowly, and in which neither cognitive decline nor dementia are prominent. These findin gs suggest a direct relation between SNCA gene dosage and disease progression.
文摘The α synuclein gene (SNCA) has been implicated in autosomal dominant forms of Parkinsons disease. We screened 119 individuals from families with this rar e form of the disease for SNCA duplications by semiquantitative multiplex PCR. T wo patients had duplications, which were confirmed by analysis of intragenic and flanking microsatellite markers. The phenotype in both patients was indistingui shable from idiopathic Parkinsons disease and no atypical features were presen t, by contrast with reports of families with triplication of the same gene. Thes e results indicate that SNCA is more frequently associated with familial Parkins ons disease than previously thought, and that there is a clear dosage effect a ccording to the number of supernumerary copies of this gene.
文摘Background and aim: The importance of the hepatocyte ploidisation pattern to the control of cell proliferation and differentiation has been well established. However, there are no data that have characterised hepatocyte ploidy at various stages of chronic liver inflammation and fibrosis in vivo. Methods: We therefore investigated hepatocyte ploidy/binuclearity patterns in 57 patients with chronic hepatitis, using a recently developed methodology which allows simultaneous hepatocyte ploidy and binuclearity analyses on the same liver section. Results: The percentage of mononuclear diploid hepatocytes was significantly reduced in patients with high hepatitis activity and marked fibrosis (low activity: 75.1 (18.8)%v high activity: 61.8 (21.6)%, p = 0.0111, and low fibrosis: 77.3 (13.8)%v high fibrosis: 57.4 (23.3)%, p = 0.0002). Accordingly, the percentage of mononuclear polyploid hepatocytes increased in patients with high hepatitis activity and marked fibrosis (low activity: 11.9 (15.5)%v high activity: 22.2 (20.1)%, p = 0.0166, and low fibrosis: 9.4 (10.7)%v high fibrosis: 26.4 (21.6)%, p = 0.0001). In addition, the fraction of binuclear hepatocytes was significantly higher in patients with hepatitis B virus (HBV) than in those with hepatitis C virus (HCV) infections (HBV: 18.2 (7.6)%v HCV: 12.0 (4.8)%; p = 0.0020). Under multivariate analysis, HBV infection was an independent factor accounting for the larger binuclear hepatocyte fraction (p = 0.0294). Conclusion: Our results revealed an increase in the polyploid hepatocyte fraction which correlates with the severity of chronic hepatitis; moreover, we demonstrated that HBV and HCV related chronic hepatitis exhibited distinctive hepatocyte ploidy patterns, thus allowing the suggestion that these two viral infections may modulate liver ploidy through different mechanisms.
