AIM: To determine the composition of both fecal and duodenal mucosa-associated microbiota in irritable bowel syndrome (IBS) patients and healthy subjects using molecular-based techniques. METHODS: Fecal and duodenal m...AIM: To determine the composition of both fecal and duodenal mucosa-associated microbiota in irritable bowel syndrome (IBS) patients and healthy subjects using molecular-based techniques. METHODS: Fecal and duodenal mucosa brush samples were obtained from 41 IBS patients and 26 healthy subjects. Fecal samples were analyzed for the composition of the total microbiota using fluorescent in situ hybridization (FISH) and both fecal and duodenal brush samples were analyzed for the composition of bif idobacteria using real-time polymerase chain reaction. RESULTS: The FISH analysis of fecal samples revealed a 2-fold decrease in the level of bifidobacteria (4.2 ± 1.3 vs 8.3 ± 1.9, P < 0.01) in IBS patients compared to healthy subjects, whereas no major differences in other bacterial groups were observed. At the species level, Bifidobacterium catenulatum levels were significantly lower (6 ± 0.6 vs 19 ± 2.5, P < 0.001) in the IBS patients in both fecal and duodenal brush samples than in healthy subjects.CONCLUSION: Decreased bifidobacteria levels in both fecal and duodenal brush samples of IBS patients compared to healthy subjects indicate a role for microbiotic composition in IBS pathophysiology.展开更多
Comprehensive utilization of low grade manganese?zinc compound ore containing lead and silver with a method of reductive acid leaching was studied.According to theφ?pH diagram of Mn?Zn?H2O system,Mn and Zn can be lea...Comprehensive utilization of low grade manganese?zinc compound ore containing lead and silver with a method of reductive acid leaching was studied.According to theφ?pH diagram of Mn?Zn?H2O system,Mn and Zn can be leached simultaneously in the pH range of?2to5.61.The results showed that both hydrogen peroxide and sucrose were effective reductants which could intensify the simultaneous leaching of Mn and Zn into leachate as well as enrich Pb and Ag in the residue.95.88%of Mn and99.23%of Zn were extracted when the compound ore was leached with hydrogen peroxide in sulfuric acid media,meanwhile the contents of Pb and Ag in the residue were enriched to13.21%and489.36g/t,respectively.When sucrose was used as the reductant,the leaching efficiencies of Mn and Zn separately achieved98.26%and99.62%,and contents of Pb and Ag in the residue were as high as13.92%and517.87g/t,respectively.展开更多
The doubly curved shell(DCS)is a common structure in the engineering field.In a thermal environment,the vibration characteristics of the DCS will be affected by the thermal effect.The research on the vibration charact...The doubly curved shell(DCS)is a common structure in the engineering field.In a thermal environment,the vibration characteristics of the DCS will be affected by the thermal effect.The research on the vibration characteristics of DCS in thermal environment is relatively limited.In this paper,the thermal strain and the change of Young’s modulus caused by the changing of temperature are studied,and the DCS energy equation is established systematically.The displacement tolerance function of the DCS is constructed by the spectral geometry method,and the natural frequencies and mode shapes of the DCS with different structural parameters,such as thicknesses,ratios of R_(a)/R_(b) and a/b,at different temperatures are solved by the Rayleigh-Ritz method.The results show that the natural frequency of the DCS decreases with the increasing temperature,R_(a)/R_(b) and a/b ratios,and increases with the increasing thickness.展开更多
The utilization of wind generation equipment, such as DFIGs （double fed induction generators）, interconnected to islanded power generation and distribution systems is investigated in order to determine their effects...The utilization of wind generation equipment, such as DFIGs （double fed induction generators）, interconnected to islanded power generation and distribution systems is investigated in order to determine their effects on the overall system operating characteristics and stability. The use of a stable power station （with high speed machines） will be critical in achieving fast and reliable transient response to network events, in particular, when large transient loads are expected on a continuous basis, i.e., industrial mining and mineral processing equipment. Simulation results of this paper assist in understanding how small power stations and wind generation equipment respond to large transients in an islanded network. In particular, detailed simulations and analyses will be presented on impacts of distributed wind generation units （1.5 MW DF1G） on the stability of a small weak network. The novelty of this paper is on detailed analyses and simulation of weak networks with interconnects DFIG＇s including their impacts on system stability under various transient operating conditions.展开更多
Sweepovirus is an important monopartite begomovirus that infects plants of the genus Ipomoea worldwide. Development of artificial infection methods for sweepovirus using agroinoculation is a highly efficient means of ...Sweepovirus is an important monopartite begomovirus that infects plants of the genus Ipomoea worldwide. Development of artificial infection methods for sweepovirus using agroinoculation is a highly efficient means of studying infectivity in sweet potato. Unlike other begomoviruses, it has proven difficult to infect sweet potato plants with sweepoviruses using infectious clones. A novel sweepovirus, called Sweet potato leaf curl virus-Jiangsu(SPLCV-JS), was recently identified in China. In addition, the infectivity of the SPLCV-JS clone has been demonstrated in Nicotiana benthamiana. Here we describe the agroinfection of the sweet potato cultivar Xushu 22 with the SPLCV-JS infectious clone using vacuum infiltration. Yellowing symptoms were observed in newly emerged leaves. Molecular analysis confirmed successful inoculation by the detection of viral DNA. A synergistic effect of SPLCV-JS and the heterologous betasatellite DNA-β of Tomato yellow leaf curl China virus isolate Y10(TYLCCNV-Y10) on enhanced symptom severity and viral DNA accumulation was confirmed. The development of a routine agroinoculation system in sweet potato with SPLCV-JS using vacuum infiltration should facilitate the molecular study of sweepovirus in this host and permit the evaluation of virus resistance of sweet potato plants in breeding programs.展开更多
This article describes some dynamic aspects on dynastic utility incorporating two-sided altruism with an OLG setting. The special case is analyzed where the weights of two-sided altruism are dynamically inconsistent. ...This article describes some dynamic aspects on dynastic utility incorporating two-sided altruism with an OLG setting. The special case is analyzed where the weights of two-sided altruism are dynamically inconsistent. The Bellman equation for two-sided altruism proves to be reduced to one-sided dynamic problem, but the effective discount factor is different only in the current generation. It is shown that a contraction mapping result of value function cannot be achieved in general, and that there can locally exist an infinite number of self-consistent policy functions of the class C＂ with distinct steady states （indeterminacy of self-consistent, differentiable policy functions）.展开更多
文摘AIM: To determine the composition of both fecal and duodenal mucosa-associated microbiota in irritable bowel syndrome (IBS) patients and healthy subjects using molecular-based techniques. METHODS: Fecal and duodenal mucosa brush samples were obtained from 41 IBS patients and 26 healthy subjects. Fecal samples were analyzed for the composition of the total microbiota using fluorescent in situ hybridization (FISH) and both fecal and duodenal brush samples were analyzed for the composition of bif idobacteria using real-time polymerase chain reaction. RESULTS: The FISH analysis of fecal samples revealed a 2-fold decrease in the level of bifidobacteria (4.2 ± 1.3 vs 8.3 ± 1.9, P < 0.01) in IBS patients compared to healthy subjects, whereas no major differences in other bacterial groups were observed. At the species level, Bifidobacterium catenulatum levels were significantly lower (6 ± 0.6 vs 19 ± 2.5, P < 0.001) in the IBS patients in both fecal and duodenal brush samples than in healthy subjects.CONCLUSION: Decreased bifidobacteria levels in both fecal and duodenal brush samples of IBS patients compared to healthy subjects indicate a role for microbiotic composition in IBS pathophysiology.
基金Projects(51574284,51504293)supported by the National Natural Science Foundation of ChinaProject(2013IB020)supported by the Science and Technology Program of Yunnan Province,ChinaProject(CSUZC201606)supported by the Open-End Fund for the Valuable and Precision Instruments of Central South University,China
文摘Comprehensive utilization of low grade manganese?zinc compound ore containing lead and silver with a method of reductive acid leaching was studied.According to theφ?pH diagram of Mn?Zn?H2O system,Mn and Zn can be leached simultaneously in the pH range of?2to5.61.The results showed that both hydrogen peroxide and sucrose were effective reductants which could intensify the simultaneous leaching of Mn and Zn into leachate as well as enrich Pb and Ag in the residue.95.88%of Mn and99.23%of Zn were extracted when the compound ore was leached with hydrogen peroxide in sulfuric acid media,meanwhile the contents of Pb and Ag in the residue were enriched to13.21%and489.36g/t,respectively.When sucrose was used as the reductant,the leaching efficiencies of Mn and Zn separately achieved98.26%and99.62%,and contents of Pb and Ag in the residue were as high as13.92%and517.87g/t,respectively.
基金supported by the National Natural Science Foundation of China(No.51805341)the Natural Science Foundation of Jiangsu Province(No.BK20180843).
文摘The doubly curved shell(DCS)is a common structure in the engineering field.In a thermal environment,the vibration characteristics of the DCS will be affected by the thermal effect.The research on the vibration characteristics of DCS in thermal environment is relatively limited.In this paper,the thermal strain and the change of Young’s modulus caused by the changing of temperature are studied,and the DCS energy equation is established systematically.The displacement tolerance function of the DCS is constructed by the spectral geometry method,and the natural frequencies and mode shapes of the DCS with different structural parameters,such as thicknesses,ratios of R_(a)/R_(b) and a/b,at different temperatures are solved by the Rayleigh-Ritz method.The results show that the natural frequency of the DCS decreases with the increasing temperature,R_(a)/R_(b) and a/b ratios,and increases with the increasing thickness.
文摘The utilization of wind generation equipment, such as DFIGs （double fed induction generators）, interconnected to islanded power generation and distribution systems is investigated in order to determine their effects on the overall system operating characteristics and stability. The use of a stable power station （with high speed machines） will be critical in achieving fast and reliable transient response to network events, in particular, when large transient loads are expected on a continuous basis, i.e., industrial mining and mineral processing equipment. Simulation results of this paper assist in understanding how small power stations and wind generation equipment respond to large transients in an islanded network. In particular, detailed simulations and analyses will be presented on impacts of distributed wind generation units （1.5 MW DF1G） on the stability of a small weak network. The novelty of this paper is on detailed analyses and simulation of weak networks with interconnects DFIG＇s including their impacts on system stability under various transient operating conditions.
基金supported by grants from the National High Technology Research and Development Program of China (2012AA101204)Shanghai Municipal Afforestation and City Appearance and Environmental Sanitation Administration (G102410, F132427)
文摘Sweepovirus is an important monopartite begomovirus that infects plants of the genus Ipomoea worldwide. Development of artificial infection methods for sweepovirus using agroinoculation is a highly efficient means of studying infectivity in sweet potato. Unlike other begomoviruses, it has proven difficult to infect sweet potato plants with sweepoviruses using infectious clones. A novel sweepovirus, called Sweet potato leaf curl virus-Jiangsu(SPLCV-JS), was recently identified in China. In addition, the infectivity of the SPLCV-JS clone has been demonstrated in Nicotiana benthamiana. Here we describe the agroinfection of the sweet potato cultivar Xushu 22 with the SPLCV-JS infectious clone using vacuum infiltration. Yellowing symptoms were observed in newly emerged leaves. Molecular analysis confirmed successful inoculation by the detection of viral DNA. A synergistic effect of SPLCV-JS and the heterologous betasatellite DNA-β of Tomato yellow leaf curl China virus isolate Y10(TYLCCNV-Y10) on enhanced symptom severity and viral DNA accumulation was confirmed. The development of a routine agroinoculation system in sweet potato with SPLCV-JS using vacuum infiltration should facilitate the molecular study of sweepovirus in this host and permit the evaluation of virus resistance of sweet potato plants in breeding programs.
文摘This article describes some dynamic aspects on dynastic utility incorporating two-sided altruism with an OLG setting. The special case is analyzed where the weights of two-sided altruism are dynamically inconsistent. The Bellman equation for two-sided altruism proves to be reduced to one-sided dynamic problem, but the effective discount factor is different only in the current generation. It is shown that a contraction mapping result of value function cannot be achieved in general, and that there can locally exist an infinite number of self-consistent policy functions of the class C＂ with distinct steady states （indeterminacy of self-consistent, differentiable policy functions）.
