The cylindrical worm processed by annular grinding wheel envelope in two degree of freedom motion state is a novel worm. This paper explains the shaping principle of such a worm. To improve meshing quality and the p...The cylindrical worm processed by annular grinding wheel envelope in two degree of freedom motion state is a novel worm. This paper explains the shaping principle of such a worm. To improve meshing quality and the properties of contact and lubrication, the multi objective optimization has been conducted for the first time to the parameters of such a worm pair by the fuzzy optimal method. The results show that, the shape of the contact line is visibly more sloped than before being optimized, lubrication angle is apparently bigger, and the distribution of contact lines is much improved.展开更多
High-speed vehicle dynamic envelope curve is defined as the maximum limit outline affected by a variety of adverse factors while the train is running. Considering the difficulties in the current measurement system suc...High-speed vehicle dynamic envelope curve is defined as the maximum limit outline affected by a variety of adverse factors while the train is running. Considering the difficulties in the current measurement system such as complicated calibration process,cumbersome aided-instruments,strict limitation of working distance, this paper carries out an optical method in which two high-speed cameras with variable-zoom lenses are adopted as binocular stereo sensors of measurement system and a high-ac-curacy 3D target with fast reconstruction is designed. The intrinsic parameters of the sensors and the relative positions between coordinate systems are solved by the method of colinearity constrained optimization algorithm. The calibration process is easy to operate and the device is also of portability. Most importantly, the severe working distance limitation between sensors and measured body is solved, enhancing the adaptability of measurement system to environment. Experimental results show that when the sensors are in the range of 8 -16 m away from the measured body, system accuracy can reach up to ±0. 5 mm, which meets the requirements to measure the dynamic envelope curve of high-speed vehicle.展开更多
In the present research,enzyme encapsulated hydrogels（single gels and double network gels）and enzyme immobilized magnetic beads,which allow high-throughput screening,were fabricated and evaluated in terms of the pre...In the present research,enzyme encapsulated hydrogels（single gels and double network gels）and enzyme immobilized magnetic beads,which allow high-throughput screening,were fabricated and evaluated in terms of the preservation,precision, and repeatability of enzyme activity.The fabricated gels and magnetic beads were analyzed in a 96-well microassay plate.Trypsin was successfully encapsulated in both types of gels and immobilized to the magnetic beads.However,pepsin,either encapsulated in the gels or immobilized to the magnetic beads,could not react with its substrates.The adaptability to various enzymes （e.g.,trypsin,β-glucuronidase,and CYP1A1）in the single gels and magnetic beads was superior to that in double network gels.However,the soak out of the enzymes was observed in the single gels.The double network gels could encapsulate trypsin,whereas the fabrication of the other enzymes（e.g.β-glucuronidase,CYP1A1,and pepsin）failed because of the inactivation of the enzymes by acryl amide and ammonium peroxodisulfate,which are the components of the gel formulation. The enzyme reaction in the magnetic beads exhibited the highest efficiency among the three fabrication methods.Furthermore, the stability of the enzymes immobilized to the magnetic beads was better than that fabricated by the other methods,and the activities of trypsin andβ-glucuronidase did not decline for up to one week.In addition,in the magnetic beads,the activities of trypsin andβ-glucuronidase can be well repeated.Hence,although the adaptability of the double network gels to various enzymes is currently limited,the efficiency of the enzyme encapsulation can be improved by optimizing the formulation of acryl amide gels.展开更多
文摘The cylindrical worm processed by annular grinding wheel envelope in two degree of freedom motion state is a novel worm. This paper explains the shaping principle of such a worm. To improve meshing quality and the properties of contact and lubrication, the multi objective optimization has been conducted for the first time to the parameters of such a worm pair by the fuzzy optimal method. The results show that, the shape of the contact line is visibly more sloped than before being optimized, lubrication angle is apparently bigger, and the distribution of contact lines is much improved.
基金National Science and Technology Major Project(No.2016ZX04003001)
文摘High-speed vehicle dynamic envelope curve is defined as the maximum limit outline affected by a variety of adverse factors while the train is running. Considering the difficulties in the current measurement system such as complicated calibration process,cumbersome aided-instruments,strict limitation of working distance, this paper carries out an optical method in which two high-speed cameras with variable-zoom lenses are adopted as binocular stereo sensors of measurement system and a high-ac-curacy 3D target with fast reconstruction is designed. The intrinsic parameters of the sensors and the relative positions between coordinate systems are solved by the method of colinearity constrained optimization algorithm. The calibration process is easy to operate and the device is also of portability. Most importantly, the severe working distance limitation between sensors and measured body is solved, enhancing the adaptability of measurement system to environment. Experimental results show that when the sensors are in the range of 8 -16 m away from the measured body, system accuracy can reach up to ±0. 5 mm, which meets the requirements to measure the dynamic envelope curve of high-speed vehicle.
基金The Global COE Program from the Ministry of Education,Science,Sports,and Culture of Japan.
文摘In the present research,enzyme encapsulated hydrogels（single gels and double network gels）and enzyme immobilized magnetic beads,which allow high-throughput screening,were fabricated and evaluated in terms of the preservation,precision, and repeatability of enzyme activity.The fabricated gels and magnetic beads were analyzed in a 96-well microassay plate.Trypsin was successfully encapsulated in both types of gels and immobilized to the magnetic beads.However,pepsin,either encapsulated in the gels or immobilized to the magnetic beads,could not react with its substrates.The adaptability to various enzymes （e.g.,trypsin,β-glucuronidase,and CYP1A1）in the single gels and magnetic beads was superior to that in double network gels.However,the soak out of the enzymes was observed in the single gels.The double network gels could encapsulate trypsin,whereas the fabrication of the other enzymes（e.g.β-glucuronidase,CYP1A1,and pepsin）failed because of the inactivation of the enzymes by acryl amide and ammonium peroxodisulfate,which are the components of the gel formulation. The enzyme reaction in the magnetic beads exhibited the highest efficiency among the three fabrication methods.Furthermore, the stability of the enzymes immobilized to the magnetic beads was better than that fabricated by the other methods,and the activities of trypsin andβ-glucuronidase did not decline for up to one week.In addition,in the magnetic beads,the activities of trypsin andβ-glucuronidase can be well repeated.Hence,although the adaptability of the double network gels to various enzymes is currently limited,the efficiency of the enzyme encapsulation can be improved by optimizing the formulation of acryl amide gels.
