Ovomermis sinensis (Nematode,Mermithidae) is a parasitic nematode of ar myworm. But during its culture in vitro and rearing in vivo, we found that we co uld not attain the suitable proportion of male and female nemato...Ovomermis sinensis (Nematode,Mermithidae) is a parasitic nematode of ar myworm. But during its culture in vitro and rearing in vivo, we found that we co uld not attain the suitable proportion of male and female nematodes. In order to disclose the reason why the number of the filial generation was imbalanced betw een two sexes and get basic data on genetic development, we analyzed dissoluble proteins of Ovomermis sinensis imagoes with electrophoresis. Firstly, throug h SD S-PAGE, we found more than three specific proteins were detected in female imag o with the approximate molecular weight of 15-16 kD, 21-24 kD, 72-76 kD and on e abou t 26 kD specific protein in male imago. Secondly, we used a new improved two-di me nsional electrophoresis method to analyze the proteins and found that the result acco rds with those of SDS-PAGE, which showed that this 2-DE method is practicable. A lso, in this 2-DE, proteins can be well separated and plenty of different pr oteins can be clearly displayed .展开更多
用焦锑酸钾沉淀法对45℃和28℃下黄瓜(新泰密刺)授粉后柱头及子房中C a2+分布进行了电镜观察,并采用酶联免疫吸附测定法(EL ISA)测定了叶片中的ABA含量及用等电聚焦聚丙烯酰胺双向电泳(IEF-SDS PAGE)方法对其蛋白合成的变化进行了研究....用焦锑酸钾沉淀法对45℃和28℃下黄瓜(新泰密刺)授粉后柱头及子房中C a2+分布进行了电镜观察,并采用酶联免疫吸附测定法(EL ISA)测定了叶片中的ABA含量及用等电聚焦聚丙烯酰胺双向电泳(IEF-SDS PAGE)方法对其蛋白合成的变化进行了研究.结果表明,28℃时柱头中的C a2+主要分布在细胞间隙中,经45℃处理后细胞间隙和胞内C a2+水平均显著升高,胞内外C a2+浓度梯度逐步丧失;45℃处理1 h后柱头和子房中内源ABA含量显著提高;高温处理后柱头和子房中产生了一些新的蛋白质,如柱头中的(MW26.0 kD,P I 5.4)、(MW90.0 kD,P I5.1)、(MW54.0 kD,P I 4.6)、(MW41.0 kD,P I 6.2),子房中的(MW90.0 kD,P I5.2)、(MW61.0 kD,P I 5.4)、(MW48.0 kD,P I 6.4)、(MW40.5 kD,P I 4.9);另有一些蛋白质与常温相比表达量大幅上调,如柱头中的(MW67.0kD,P I 5.8)、(MW56.5 kD,P I 5.8),子房中的(MW70.0 kD,P I 5.7)、(MW57.0 kD,P I 5.7).上述结果表明,C a2+和ABA信号系统均参与了授粉后黄瓜雌性器官对高温胁迫的反应调节并最终导致基因表达发生变化.展开更多
文摘Ovomermis sinensis (Nematode,Mermithidae) is a parasitic nematode of ar myworm. But during its culture in vitro and rearing in vivo, we found that we co uld not attain the suitable proportion of male and female nematodes. In order to disclose the reason why the number of the filial generation was imbalanced betw een two sexes and get basic data on genetic development, we analyzed dissoluble proteins of Ovomermis sinensis imagoes with electrophoresis. Firstly, throug h SD S-PAGE, we found more than three specific proteins were detected in female imag o with the approximate molecular weight of 15-16 kD, 21-24 kD, 72-76 kD and on e abou t 26 kD specific protein in male imago. Secondly, we used a new improved two-di me nsional electrophoresis method to analyze the proteins and found that the result acco rds with those of SDS-PAGE, which showed that this 2-DE method is practicable. A lso, in this 2-DE, proteins can be well separated and plenty of different pr oteins can be clearly displayed .
文摘用焦锑酸钾沉淀法对45℃和28℃下黄瓜(新泰密刺)授粉后柱头及子房中C a2+分布进行了电镜观察,并采用酶联免疫吸附测定法(EL ISA)测定了叶片中的ABA含量及用等电聚焦聚丙烯酰胺双向电泳(IEF-SDS PAGE)方法对其蛋白合成的变化进行了研究.结果表明,28℃时柱头中的C a2+主要分布在细胞间隙中,经45℃处理后细胞间隙和胞内C a2+水平均显著升高,胞内外C a2+浓度梯度逐步丧失;45℃处理1 h后柱头和子房中内源ABA含量显著提高;高温处理后柱头和子房中产生了一些新的蛋白质,如柱头中的(MW26.0 kD,P I 5.4)、(MW90.0 kD,P I5.1)、(MW54.0 kD,P I 4.6)、(MW41.0 kD,P I 6.2),子房中的(MW90.0 kD,P I5.2)、(MW61.0 kD,P I 5.4)、(MW48.0 kD,P I 6.4)、(MW40.5 kD,P I 4.9);另有一些蛋白质与常温相比表达量大幅上调,如柱头中的(MW67.0kD,P I 5.8)、(MW56.5 kD,P I 5.8),子房中的(MW70.0 kD,P I 5.7)、(MW57.0 kD,P I 5.7).上述结果表明,C a2+和ABA信号系统均参与了授粉后黄瓜雌性器官对高温胁迫的反应调节并最终导致基因表达发生变化.
