AIM： To search for and validate differentially expressed proteins in patients with gastric adenocarcinoma.METHODS： We used two-dimensional gel electrophoresis and mass spectrometry to search for differentially expre...AIM： To search for and validate differentially expressed proteins in patients with gastric adenocarcinoma.METHODS： We used two-dimensional gel electrophoresis and mass spectrometry to search for differentially expressed proteins in patients with gastric adenocarcinoma. A set of proteins was validated with immunoblotting.RESULTS： We identified 30 different proteins involved in various biological processes： metabolism, development, death, response to stress, cell cycle, cell communication, transport, and cell motility. Eight proteinswere chosen for further validation by immunoblotting. Our results show that gastrokine-1, 39S ribosomal protein L12 （mitochondrial precursor）, plasma cell-induced resident endoplasmic reticulum protein, and glutathione S-transferase mu 3 were significantly underexpressed in gastric adenocarcinoma relative to adjacent non-tumor tissue samples. On the other hand, sep- tin-2, ubiquitin-conjugating enzyme E2 N, and transaldolase were significantly overexpressed. Translationally controlled tumor protein was shown to be differentially expressed only in patients with cancer of the gastric cardia/esophageal border.CONCLUSION： This work presents a set of possible diagnostic biomarkers, validated for the first time. It might contribute to the efforts of understanding gastric cancer carcinogenesis,展开更多
Today, the most commonly used bi-directional airfoil in hydraulic machinery is the S-shaped airfoil(SA). A new bi-directional airfoil, obtained by lowering the bent airfoil camber and thickening the bone line bi-direc...Today, the most commonly used bi-directional airfoil in hydraulic machinery is the S-shaped airfoil(SA). A new bi-directional airfoil, obtained by lowering the bent airfoil camber and thickening the bone line bi-directionally, is proposed. Two bi-directional axial flow pumps, with the SA blades and the arc airfoil(AA) blades, respectively, were designed to verify the applicability of this airfoil. By comparing the hydraulic performances of the two pumps obtained from the experiments, and the cavitation performance and flow field of the two pumps obtained from numerical simulations, it was found that AA blades can improve both the hydraulic and cavitation performances under a low flow rate and near the best efficiency flow point(BEP), whether in forward or reverse operation for the bi-directional axial-flow pump. Furthermore, the hydraulic performance of the AA blade pump declines sharply in a large flow rate compared to that of the SA blade pump. The location and composition of the inhomogeneous flows inside the impellers of the two pumps are similar. However, the SA blades work on fluid, mainly at the front half of the blades, which is a distinctive feature from the AA blades and may be a universal phenomenon in all SA-type blades.展开更多
基金Supported by Research grant,No.P1-0104-0386the Junior Researcher Grant,No.1000-07-310086,awarded to Kocevar N,both from the Slovenian Research Agency
文摘AIM： To search for and validate differentially expressed proteins in patients with gastric adenocarcinoma.METHODS： We used two-dimensional gel electrophoresis and mass spectrometry to search for differentially expressed proteins in patients with gastric adenocarcinoma. A set of proteins was validated with immunoblotting.RESULTS： We identified 30 different proteins involved in various biological processes： metabolism, development, death, response to stress, cell cycle, cell communication, transport, and cell motility. Eight proteinswere chosen for further validation by immunoblotting. Our results show that gastrokine-1, 39S ribosomal protein L12 （mitochondrial precursor）, plasma cell-induced resident endoplasmic reticulum protein, and glutathione S-transferase mu 3 were significantly underexpressed in gastric adenocarcinoma relative to adjacent non-tumor tissue samples. On the other hand, sep- tin-2, ubiquitin-conjugating enzyme E2 N, and transaldolase were significantly overexpressed. Translationally controlled tumor protein was shown to be differentially expressed only in patients with cancer of the gastric cardia/esophageal border.CONCLUSION： This work presents a set of possible diagnostic biomarkers, validated for the first time. It might contribute to the efforts of understanding gastric cancer carcinogenesis,
基金supported by the Production,Education and Scientific Research Program of the Baoshan District,Shanghai City(Grant No.CXY2012-12)
文摘Today, the most commonly used bi-directional airfoil in hydraulic machinery is the S-shaped airfoil(SA). A new bi-directional airfoil, obtained by lowering the bent airfoil camber and thickening the bone line bi-directionally, is proposed. Two bi-directional axial flow pumps, with the SA blades and the arc airfoil(AA) blades, respectively, were designed to verify the applicability of this airfoil. By comparing the hydraulic performances of the two pumps obtained from the experiments, and the cavitation performance and flow field of the two pumps obtained from numerical simulations, it was found that AA blades can improve both the hydraulic and cavitation performances under a low flow rate and near the best efficiency flow point(BEP), whether in forward or reverse operation for the bi-directional axial-flow pump. Furthermore, the hydraulic performance of the AA blade pump declines sharply in a large flow rate compared to that of the SA blade pump. The location and composition of the inhomogeneous flows inside the impellers of the two pumps are similar. However, the SA blades work on fluid, mainly at the front half of the blades, which is a distinctive feature from the AA blades and may be a universal phenomenon in all SA-type blades.
