[Objective] The paper aimed at researching lectins in muscles of Varicorhinus macrolepis and providing scientific basis for researching the adaptation mechanism and immune response of V.macrolepis to environment,which...[Objective] The paper aimed at researching lectins in muscles of Varicorhinus macrolepis and providing scientific basis for researching the adaptation mechanism and immune response of V.macrolepis to environment,which were advantageous for the protection and reproduction of V.macrolepis.[Method] V.macrolepis was used as test materials for the hemagglutination test by dialdehyde fixation to prove the existence of lectins in muscle crude homogenate of Varicorhinus macrolepis and study the physical and chemical characters.[Result] Lectins in muscle crude homogenate of V.macrolepis had shown hemagglutination effects on erythrocytes of six types of animals and had the maximum hemagglutination activity against rabbit erythrocytes,which belonged to the S-type lectins with optimal pH ranged from 4 to 8 and optimal temperature at 60 ℃.Results from the saccharide inhibition test had indicated that the sucrose was the only kind of saccharide which had inhibited the hemagglutination,suggesting that sucrose had played an important role in the process of recognition and aggregation of lectins.[Conclusion] It had been speculated that the optimal pH ranges for thermal sensitivity and hemagglutination activity of lectins in different types of aquatic organisms were similar.展开更多
The purpose of the present paper is twofold. First, the projective Riccati equations (PREs for short) are resolved by means of a linearized theorem, which was known in the literature. Based on the signs and values o...The purpose of the present paper is twofold. First, the projective Riccati equations (PREs for short) are resolved by means of a linearized theorem, which was known in the literature. Based on the signs and values of coeffcients of PREs, the solutions with two arbitrary parameters of PREs can be expressed by the hyperbolic functions, the trigonometric functions, and the rational functions respectively, at the same time the relation between the components of each solution to PREs is also implemented. Second, more new travelling wave solutions for some nonlinear PDEs, such as the Burgers equation, the mKdV equation, the NLS^+ equation, new Hamilton amplitude equation, and so on, are obtained by using Sub-ODE method, in which PREs are taken as the Sub-ODEs. The key idea of this method is that the travelling wave solutions of nonlinear PDE can be expressed by a polynomial in two variables, which are the components of each solution to PREs, provided that the homogeneous balance between the higher order derivatives and nonlinear terms in the equation is considered.展开更多
Isolation and purification of single-walled carbon nanotubes (SWCNTs) are prerequisites for their implementation in various applications. In this work, we present a fast (-5 min), low-cost, and easily scalable ben...Isolation and purification of single-walled carbon nanotubes (SWCNTs) are prerequisites for their implementation in various applications. In this work, we present a fast (-5 min), low-cost, and easily scalable bench-top approach to the extraction of high-quality isolated SWCNTs from bundles and impurities in an aqueous dispersion. The extraction procedure, based on aqueous two-phase (ATP) separation, is widely applicable to any SWCNT source (tested on samples up to 1.7 nm in diameter) and independent of defect density, purity, diameter, and length. The extracted dispersions demonstrate that the removal of large aggregates, small bundles, and impurities is comparable to that by density gradient ultracentrifugation, but without the need for high-end instrumentation. Raman and fluorescence-excitation spectroscopy, single-nanotube fluorescence imaging, atomic force and transmission electron microscopy, and thermogravimetric analysis all confirm the high purity of the isolated SWCNTs. By predispersing the SWCNTs without sonication (only gentle stirring), full-length, pristine SWCNTs can be isolated (tested up to 20 μm). Hence, this simple ATP method will find immediate application in the generation of SWCNT materials for all levels of nanotube research and applications, from fundamental studies to high-performance devices.展开更多
基金Supported by National Natural Science Foundation of China(3070007131172074)National Natural Science Foundation of Shandong Province(ZR2010CL002)~~
文摘[Objective] The paper aimed at researching lectins in muscles of Varicorhinus macrolepis and providing scientific basis for researching the adaptation mechanism and immune response of V.macrolepis to environment,which were advantageous for the protection and reproduction of V.macrolepis.[Method] V.macrolepis was used as test materials for the hemagglutination test by dialdehyde fixation to prove the existence of lectins in muscle crude homogenate of Varicorhinus macrolepis and study the physical and chemical characters.[Result] Lectins in muscle crude homogenate of V.macrolepis had shown hemagglutination effects on erythrocytes of six types of animals and had the maximum hemagglutination activity against rabbit erythrocytes,which belonged to the S-type lectins with optimal pH ranged from 4 to 8 and optimal temperature at 60 ℃.Results from the saccharide inhibition test had indicated that the sucrose was the only kind of saccharide which had inhibited the hemagglutination,suggesting that sucrose had played an important role in the process of recognition and aggregation of lectins.[Conclusion] It had been speculated that the optimal pH ranges for thermal sensitivity and hemagglutination activity of lectins in different types of aquatic organisms were similar.
基金The project supported in part by the Natural Science Foundation of Education Department of Henan Province of China under Grant No. 2006110002 and the Science Foundations of Henan University of Science and Technology under Grant Nos. 2004ZD002 and 2006ZY001
文摘The purpose of the present paper is twofold. First, the projective Riccati equations (PREs for short) are resolved by means of a linearized theorem, which was known in the literature. Based on the signs and values of coeffcients of PREs, the solutions with two arbitrary parameters of PREs can be expressed by the hyperbolic functions, the trigonometric functions, and the rational functions respectively, at the same time the relation between the components of each solution to PREs is also implemented. Second, more new travelling wave solutions for some nonlinear PDEs, such as the Burgers equation, the mKdV equation, the NLS^+ equation, new Hamilton amplitude equation, and so on, are obtained by using Sub-ODE method, in which PREs are taken as the Sub-ODEs. The key idea of this method is that the travelling wave solutions of nonlinear PDE can be expressed by a polynomial in two variables, which are the components of each solution to PREs, provided that the homogeneous balance between the higher order derivatives and nonlinear terms in the equation is considered.
文摘Isolation and purification of single-walled carbon nanotubes (SWCNTs) are prerequisites for their implementation in various applications. In this work, we present a fast (-5 min), low-cost, and easily scalable bench-top approach to the extraction of high-quality isolated SWCNTs from bundles and impurities in an aqueous dispersion. The extraction procedure, based on aqueous two-phase (ATP) separation, is widely applicable to any SWCNT source (tested on samples up to 1.7 nm in diameter) and independent of defect density, purity, diameter, and length. The extracted dispersions demonstrate that the removal of large aggregates, small bundles, and impurities is comparable to that by density gradient ultracentrifugation, but without the need for high-end instrumentation. Raman and fluorescence-excitation spectroscopy, single-nanotube fluorescence imaging, atomic force and transmission electron microscopy, and thermogravimetric analysis all confirm the high purity of the isolated SWCNTs. By predispersing the SWCNTs without sonication (only gentle stirring), full-length, pristine SWCNTs can be isolated (tested up to 20 μm). Hence, this simple ATP method will find immediate application in the generation of SWCNT materials for all levels of nanotube research and applications, from fundamental studies to high-performance devices.