The exact solutions of the general nonlinear dynamic system in a new double-chain model of DNA are studied by using both the Conte's Painlevé truncation expansion and the Pickering's truncation expansion....The exact solutions of the general nonlinear dynamic system in a new double-chain model of DNA are studied by using both the Conte's Painlevé truncation expansion and the Pickering's truncation expansion. The symmetric kink-kink shape excitations can be found in both the Conte's truncation expansion and the Pickering's truncation expansion. Three types of new localized excitations, the asymmetric kink-kink excitations, the soliton-kink excitation, and the kink-soliton excitations, are found by using the Pickering's nonstandard truncation expansion.展开更多
Plant RNA N-glycosidase specifically hydrolyzes the N-C glycosidic bond of a conserved adenosine in the sarcin/ricin domain of the largest RNA in ribosome, releasing an adenine base and thus inhibiting protein synthes...Plant RNA N-glycosidase specifically hydrolyzes the N-C glycosidic bond of a conserved adenosine in the sarcin/ricin domain of the largest RNA in ribosome, releasing an adenine base and thus inhibiting protein synthesis. This substrate specificity was challenged later by discovery that various RNA derivatives and DNAs, especially the double-stranded supercoiled DNA could be used as substrate by RNA N-glycosidase. Thus, it was argued whether the DNA-cleaving activity was an intrinsic feature of RNA N-glycosidase or it was contaminated by DNase. In this article, several lines of evidence are presented to show that RNA N-glycosidase can really release the adenine base from the double-stranded supercoi/ed DNA. It was proposed that the cleavage mechanism of supercoiled DNA was the phosphodiester bonds in enzymatically deadenylated regions of the supercoiled DNA would become fragile and liable to produce nicked or linear form owing to the existence of tension in the supercoiled DNA molecule, not direct result of enzymatic action on the phosphodiester bond.展开更多
基金国家杰出青年科学基金，the Research Fund for the Doctoral Program of Higher Education of China，国家自然科学基金
文摘The exact solutions of the general nonlinear dynamic system in a new double-chain model of DNA are studied by using both the Conte's Painlevé truncation expansion and the Pickering's truncation expansion. The symmetric kink-kink shape excitations can be found in both the Conte's truncation expansion and the Pickering's truncation expansion. Three types of new localized excitations, the asymmetric kink-kink excitations, the soliton-kink excitation, and the kink-soliton excitations, are found by using the Pickering's nonstandard truncation expansion.
文摘Plant RNA N-glycosidase specifically hydrolyzes the N-C glycosidic bond of a conserved adenosine in the sarcin/ricin domain of the largest RNA in ribosome, releasing an adenine base and thus inhibiting protein synthesis. This substrate specificity was challenged later by discovery that various RNA derivatives and DNAs, especially the double-stranded supercoiled DNA could be used as substrate by RNA N-glycosidase. Thus, it was argued whether the DNA-cleaving activity was an intrinsic feature of RNA N-glycosidase or it was contaminated by DNase. In this article, several lines of evidence are presented to show that RNA N-glycosidase can really release the adenine base from the double-stranded supercoi/ed DNA. It was proposed that the cleavage mechanism of supercoiled DNA was the phosphodiester bonds in enzymatically deadenylated regions of the supercoiled DNA would become fragile and liable to produce nicked or linear form owing to the existence of tension in the supercoiled DNA molecule, not direct result of enzymatic action on the phosphodiester bond.
