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海洋尖尾藻对2种海洋微藻的摄食特征研究 被引量:2
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作者 张艳苓 李雪梅 +1 位作者 李志伟 安鑫龙 《水产学报》 CAS CSCD 北大核心 2014年第4期516-524,共9页
为研究海洋尖尾藻对双色藻和齿状纹石藻的摄食特征,分别采用2种海洋微藻为饵料培养海洋尖尾藻,分析海洋尖尾藻对2种海洋微藻的摄食过程、培养液颜色变化特征和摄食规律。结果显示,海洋尖尾藻摄食双色藻时将藻丝一段一段慢慢裹入纵沟,而... 为研究海洋尖尾藻对双色藻和齿状纹石藻的摄食特征,分别采用2种海洋微藻为饵料培养海洋尖尾藻,分析海洋尖尾藻对2种海洋微藻的摄食过程、培养液颜色变化特征和摄食规律。结果显示,海洋尖尾藻摄食双色藻时将藻丝一段一段慢慢裹入纵沟,而齿状纹石藻整个细胞同时被裹入纵沟。海洋尖尾藻接种6 h后,对2种海洋微藻的摄食率均为100%。随着海洋尖尾藻细胞初始密度(双色藻培养液中分别为0.8×104、2.4×103和0.8×103个/mL,齿状纹石藻培养液中分别为3.9×103、2.4×103和1.7×103个/mL)降低,其种群在初始密度为3.4×107个/mL的双色藻培养液中达到稳定期所需时间增加,分别为接种后第3、4和6天,双色藻被摄食完毕所需时间也增加,分别为接种后第5、10和15天;海洋尖尾藻种群在初始密度为6.6×105个/mL的齿状纹石藻培养液中达到稳定期所需时间增加,分别为接种后第4、5和12天,齿状纹石藻被摄食完毕所需时间也增加,分别为接种后第6、7和13天。研究表明,海洋尖尾藻对不同大小和体制类型的双色藻和齿状纹石藻的摄食过程不同,培养方式对培养液颜色变化产生影响,饵料藻种类以及饵料藻和海洋尖尾藻最初浓度配比会影响海洋尖尾藻摄食,15 d培养过程中双色藻种群和齿状纹石藻种群均向海洋尖尾藻种群发生了演替。 展开更多
关键词 海洋尖尾藻 双色藻 齿状纹石藻 摄食过程 摄食规律 颜色变化
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Identification of the nuclear matrix and chromosome scaffold in dinoflagellate Crypthecodinium cohnii^1
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作者 CAISHUTAO CONGMEIZENG 《Cell Research》 SCIE CAS CSCD 1992年第2期165-182,共18页
Dinoflagellate is one of the primitive eukaryotes, whose nucleus may represent one of the transition stages from prokaryotic nucleoid to typical eukaryotic nucleus. Using selective extraction together with embeddment ... Dinoflagellate is one of the primitive eukaryotes, whose nucleus may represent one of the transition stages from prokaryotic nucleoid to typical eukaryotic nucleus. Using selective extraction together with embeddment - free section and whole mount electron microscopy, a delicate nuclear matrix filament network was shown, for the first time, in dinoflagellate Crypthecodinium cohnii nucleus. Chromosome residues are connected with nuclear matrix filaments to form a complete network spreading over the nucleus. Moreover, we demonstrated that the dinoflagellate chromosome retains a protein scaffold after the depletion of DNA and soluble proteins. This scaffold preserves the characteristic morphology of the chromosome. Two dimensional elec-trophoreses indicated that the nuclear matrix and chromosome scaffold are mainly composed of acidic proteins. Our results demonstrated that a framework similar to the nuclear matrix and chromosome scaffold in mammalian cells appears in this primitive eukaryote,suggesting that these structures may have been originated from the early stages of eukaryote evolution. 展开更多
关键词 nuclear matrix chromosome scaffold DINOFLAGELLATE Crypthecodinium cohnii.
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