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基于双靶点杂交链式反应的大肠杆菌核酸检测研究
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作者 扈卓然 庄妙慧 +4 位作者 杨晨 翟若男 杨倩思茹 郑雅芳 胡善文 《中国口岸科学技术》 2024年第7期89-96,共8页
本研究建立了一种以杂交链式反应为基础的双靶点检测大肠杆菌核酸的新方法。以大肠杆菌O157:H7的两段特异性核酸序列为靶标,设计了带有FAM荧光基团和猝灭基团标记的发卡探针,通过杂交链式反应扩增靶标序列,并优化了反应条件。结果表明,... 本研究建立了一种以杂交链式反应为基础的双靶点检测大肠杆菌核酸的新方法。以大肠杆菌O157:H7的两段特异性核酸序列为靶标,设计了带有FAM荧光基团和猝灭基团标记的发卡探针,通过杂交链式反应扩增靶标序列,并优化了反应条件。结果表明,在0.15 mol/L Na+盐浓度和0.02 mol/L HEPES缓冲体系下,该方法具有良好的稳定性和选择性,幽门螺旋杆菌、志贺氏菌、金黄色葡萄球菌及沙门氏菌均不产生干扰信号。检测在大肠杆菌靶标核酸浓度为0.001~20 nmol/L范围内显示出良好的线性关系,检出限为0.001 nmol/L。在实际样本检测中,该方法能准确区分真实阳性样本,有望为大肠杆菌检测提供一种新技术。 展开更多
关键词 大肠杆菌 杂交链式反应 双靶点检测
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IMAGING AND MTI PROCESSING BASED ON DUAL-FREQUENCIES DUAL-APERTURES SPACEBORNE SAR 被引量:1
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作者 Yin Jianfeng Li Daojing Wu Yirong 《Journal of Electronics(China)》 2009年第1期38-44,共7页
Based on dual-frequencies dual-apertures spaceborne SAR (Synthetic Aperture Radar), a new SAR system with four receiving channels and two operation modes is presented in this paper, SAR imaging and Moving Target Ind... Based on dual-frequencies dual-apertures spaceborne SAR (Synthetic Aperture Radar), a new SAR system with four receiving channels and two operation modes is presented in this paper, SAR imaging and Moving Target Indication (MTI) are studied in this system. High resolution imaging with wide swath is implemented by the Mode Ⅰ, and MTI is completed by the Mode Ⅱ. High azimuth resolution is achieved by the Displaced Phase Center (DPC) multibeam technique. And the Coherent Accumulation (CA) method, which combines dual channels data of different carrier frequency, is used to enhance the range resolution. For the data of different carrier frequency, the two aperture interferometric processing is executed to implement clutter cancellation, respectively. And the couple of clutter suppressed data are employed to implement Dual Carrier Frequency Conjugate Processing (DCFCP), then both slow and fast moving targets detection can be completed, followed by moving target imaging. The simulation results show the validity of the signal processing method of this new SAR system. 展开更多
关键词 Spaceborne Synthetic Aperture Radar (SAR) Dual-frequencies dual-apertures Enhancing range resolution Slow and fast moving target detection Moving target imaging
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