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双鞭甲藻液体菌种培养条件优化 被引量:1
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作者 李正鹏 尚耘 《食品工业科技》 CAS CSCD 北大核心 2010年第3期228-229,238,共3页
以微藻生物量为指标,对产DHA藻类液体培养条件进行优化。在正交实验和单因素实验的基础上,确定发酵培养条件为:葡萄糖40g/L、谷氨酸钠10g/L、蛋白胨2g/L,人工海水500mL/L,转速160r/min,初始pH6.5,接种量10%,装液量100mL/250mL,28℃培养... 以微藻生物量为指标,对产DHA藻类液体培养条件进行优化。在正交实验和单因素实验的基础上,确定发酵培养条件为:葡萄糖40g/L、谷氨酸钠10g/L、蛋白胨2g/L,人工海水500mL/L,转速160r/min,初始pH6.5,接种量10%,装液量100mL/250mL,28℃培养5d,微藻生物量达1.63g/100mL。 展开更多
关键词 双鞭甲藻 液体菌种 培养条件
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美发现来源于海洋双鞭甲藻的聚酮化合物合酶基因
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《生物技术通报》 CAS CSCD 2005年第4期80-80,共1页
Marine Biotechnology 2003年5卷1期1~12页报道:基因组水平上聚酮化合物(多聚乙酰)生物合成途径操作技术的快速发展,为建立其新的生物合成途径提供了基础。
关键词 海洋生物 双鞭甲藻 聚酮化合物合酶基因 美国 生物合成
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Identification of the nuclear matrix and chromosome scaffold in dinoflagellate Crypthecodinium cohnii^1
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作者 CAISHUTAO CONGMEIZENG 《Cell Research》 SCIE CAS CSCD 1992年第2期165-182,共18页
Dinoflagellate is one of the primitive eukaryotes, whose nucleus may represent one of the transition stages from prokaryotic nucleoid to typical eukaryotic nucleus. Using selective extraction together with embeddment ... Dinoflagellate is one of the primitive eukaryotes, whose nucleus may represent one of the transition stages from prokaryotic nucleoid to typical eukaryotic nucleus. Using selective extraction together with embeddment - free section and whole mount electron microscopy, a delicate nuclear matrix filament network was shown, for the first time, in dinoflagellate Crypthecodinium cohnii nucleus. Chromosome residues are connected with nuclear matrix filaments to form a complete network spreading over the nucleus. Moreover, we demonstrated that the dinoflagellate chromosome retains a protein scaffold after the depletion of DNA and soluble proteins. This scaffold preserves the characteristic morphology of the chromosome. Two dimensional elec-trophoreses indicated that the nuclear matrix and chromosome scaffold are mainly composed of acidic proteins. Our results demonstrated that a framework similar to the nuclear matrix and chromosome scaffold in mammalian cells appears in this primitive eukaryote,suggesting that these structures may have been originated from the early stages of eukaryote evolution. 展开更多
关键词 nuclear matrix chromosome scaffold DINOFLAGELLATE Crypthecodinium cohnii.
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