反分析技术及其在巷道稳定性分析中的实用化研究

系统阐述了反分析技术理论及实用化研究 ,并将其用于具体矿山巷道稳定性分析中的地应力和弹模的实测及计算 ,获得了满意的效果。为降低地压稳定性研究获取基础数据的实测费用 ,提供了一种简易可靠方法。

反分析技术及在武钢大冶铁矿的应用研究

本文系统阐述了反分析技术理论及实用化研究，并将其应用于矿山巷道稳定性分析中的地应力和弹模的实测及计算．获得了满意的效果。为降低地压稳定性研究中要获取基础数据而花费的实测费用，提供了一种简易可靠的方法。

隧道施工监控量测及数据反分析技术讨论

随着西部大开发的不断深入，山区当中的交通运输建设工程中，隧道的施工建设正面临着全新的机遇。在现阶段的隧道施工中，经常会运用到监控量测技术，它实际上是新奥法的重要元素之一，在实际施工中具有不可替代的重要作用。本文将高速公路贵州段某隧道工程最为研究对象，对其监控量测的方案进行了细致的分析，并在此基础上，进行了数据反分析，旨在为山区隧道工程建设的发展积累实际经验。

岩体力学参数的损伤─—反分析优化性研究

岩体力学参数一直是困扰岩石力学和数值计算深入研究的难题，它在很大程度上决定了数值计算结果的准确性、客观性和实用性。本文在岩体损伤力学描述的基础上，提出了弹性波波速、损伤力学和反分析相结合的优化方法。

用电检查中窃电与反窃电技术的分析

为进一步提高用电检查质量,落实反窃电技术,本文主要针对用电检查中的常见窃电方法;反窃电技术措施两点内容,从多个角度出发,提出具体的可行性方法,为后续的工作展开提供有效的借鉴和参考。

大坝位移监测分析及稳定性识别研究

本文应用确定性力学来分析大坝的位移监测资料，将反分析技术引入到大坝的安全施工和运行监控中并进行工程的稳定性识别评价。在反分析中作者提出了一种新的岩体多介质弹模识别方法。以湖南东江拱坝的位移监测为研究课题进行了具体的分析和稳定性评价。最后作者提出了一种简便快速的预测评价方法，两者结合应用，使监控工作既快又实用，值得现场技术人员使用。

石灰石矿山排土场滑坡治理措施的研究

某石灰石矿山的排土区域上部已经出现局部滑移,对排土场下方的耕地资源和铁路造成严重的危害。通过两种途径使得排土区域的稳定性大大提高,同时又合理利用排土区域内的有利地形,保护了耕地资源。

Numerical Approach to the Mechanism of Cellulose Pyrolysis

A detailed mechanism analysis of cellulose pyrolysis was carried out according to the previous experimental results. On the basis of the Brodio-Shafizadeh model, a modified two-stage model was proposed to simulate the formation and decomposition of active cellulose (AC) and several main organic compounds, such as levoglucosan (LG), hydroxyl-acetaldehyde (HAA), acetol and furfural etc. During pryolysis, the temperature rise of cellulose can be divided into three stages. In the second stage, cellulose undergoes a main decomposition process in which the reaction temperature remains rather low because of the endothermic cracking of glucosidic bond of AC during the formation of LG. The components density of bio-oil, including LG and other competitive compounds, increased rapidly with the increase of temperature during the first stage. However, in the main decomposition process, LG density in bio-oil had an obvious decrease, while the competitive products appeared to increase gradually, which means the ring-opening and reforming reaction of pyranoid ring are superior to LG formation in high temperature.The secondary reaction of volatile components occurs largely in gaseous phase rather than in the solid phase. Short residence time of volatile materials in high temperature region will be advantageous to a high production of LG,which may otherwise decompose quickly under high temperature. An optimum yield of LG could be obtained when radiant source temperature is in the range of 730---920K and gas residence time is less than 1 s. In addition, the reaction temperature has a stronger effect than gas residence time on the formation of HAA, acetol, formaldehyde and furfural etc.

RAPID SCREENING OF AN ARRAYED cDNA LIBRARY BY IMPROVED PCR-BASED METHOD

The present study reports an improved PCR-based technique that allows quick and effective screening of cDNA libraries. First, the cDNA library was arrayed as follow: about 3 X 10’ cDNA clones were multiplied as individual plaques on solid medium in 24-well culture dishes at 1 200 plaque forming units per well. The phage suspension of each well was transferred to an individual microcentrifuge tube in 72-tube box. Then, box pool, row pools and column pools were set up that respectively represent a 72-tube box, rows and columns within the box. To screen a specific target cDNA,primers specific for novel ESTs ob- tained in our laboratory were employed to conduct PCR in a hierarchy mode. PCR began with the box pools, resulting in the identification of some Positive box pools. Then PCR went down to the row and col- umn pools of the positive box. The intersection of the positive row (s) and column (s) revealed the candi- date positive tubes. The specificity of PCR products were meanwhile checked by restriction enzyme diges- tion. Finally, hybridization was carried out to get single specific cDNA clomes from the positive tubes. This PCR-based technique features high specificity, high efficiency and less-cost in large-scale cDNA library screening. Our initial implementation of the technique resulted in the isolation of three longer different cD- NA clones from a human fetal brain cDNA library. Thus this improved technique can serve as an alterna-tive to the time-consuming and laborious conventional hybridization-based method for screening cDNA li-brary.

Optimization design of deep lean-ore mining and safety evaluation

In order to settle the mining optimization design and safety problem of the above 1 150 m pillar of No.1 ore-body in No.H Mining Jinchuan, the lean-ore above 1 250 m, the 1 150 m horizontal pillar and the ore-body below 1 100 m regarded as research objects based on the original design project, and nine calculation schemes on different mining sequence and different fill body strength were put forward based on cement-sand ratio of 1 ： 4, 1： 12 and 1 ： 24. Calculation parameters were got by the back analysis method of field monitoring data, and the FLAC2D program was applied to compute for these schemes, stress and displacement of ground settlement, shaft and stope roof were analyzed, and some conclusions were got. Results show that the intensity of filling body and the mining technique have very important effect on controlling settlement and stability of surrounding rock; Developing of lean ore have some influences to the 16th return air filling shaft, especially for 1 500--1 400 m of the shaft; The best project is the first project. This research supply some technique references and safety appraisals for the mining of lean-ore of No.II Mining Jinchuan.

Fault Analysis of 10-kV Switch Cabinet

Considering the actual operating experience of the Daode Wind Farm, this paper analyzes the rea-sons for the fault of the lO-kV switch cabinet. Some technical improvements and management measures, such as implementing the anti-accident technical measures, improving the installation and debugging level, and strengthening operation inspection, are proposed.

Recent advance in porous coordination polymers from the viewpoint of crystalline-state transformation

Recently,research of crystalline-state transformation involving the removal/inclusion of guest molecules in porous coordination polymers(PCPs) was underway.Crystalline-state transformation,especially,single-crystal to single-crystal(SC-SC) transformation as new method for the direct observation of host-guest chemistry,can reveal the intrinsic relevance and interaction between the framework and guest molecules.This review describes our work concerning PCPs and recent investigations of others,within the last four years,from the viewpoint of crystalline-state transformations of PCPs on guest removal or inclusion processes.Ligand substitution reaction and postsynthetic modification of PCPs in SC-SC fashion which were distinguished from conventional crystalline-state transformation triggered by guest removal or exchange were highlighted in this review.The research status of crystalline-state transformation in China was briefly introduced as well.Series of structure analysis techniques including single-crystal X-ray diffraction,powder X-ray diffraction,neutron diffraction,inelastic neutron scattering as well as the application of synchrotron radiation light source will inevitably promote the advance of study of crystalline-state transformation.And as a hotspot,deep investigations of crystalline-state transformation also help us to overcome the challenge of achieving multifunction and the correlation among them,such as sorption,magnetism,optical or electrical properties simultaneously in PCPs and contribute to design stimulate-oriented porous intelligent materials in the future.

Parallel detection and quantitative analysis of specific binding of proteins by oblique-incidence reflectivity difference technique in label-free format

In this work,we parallelly detected the specific binding between microarray targets including 12 different kinds of proteins and the probe solution containing five corresponding antibodies and quantitatively analyzed the interactions between CDH13 and solution phase anti-CDH13 at six different probe concentrations by oblique-incidence reflectivity difference(OIRD)method in label-free format.The detection sensitivity reached 10 ng/mL.The experimental results indicate that the OIRD method is a promising and competing technique not only in research work but also in clinic.