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用锻造冲挤反印法修复采煤机链轮模套
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作者 隋晓堂 徐玉海 《黑龙江科技信息》 2010年第3期4-4,共1页
链牵引采煤机牵引部分上的零件链轮,是一个受牵引载荷很大的零件。由于链轮安装受空间位置的限制,结构设计体积不能太大,链轮齿面彩局部热处,工件磨损快,链轮使用寿命短,返修模套不同于一般反印法,而是利用微量变形的冲挤原理进行修复。
关键词 链轮模套 冲挤反印法修复 锻造
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反印法在胎模制造中的应用
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作者 陈国庆 《机械工人(热加工)》 2000年第11期53-53,共1页
我厂是生产破磨设备的企业,需要大量的非标准螺栓,如破碎机边护板、球磨机衬板所用的固定螺栓,以及一些精度要求不高的用于包装箱的方头螺栓(见图1),一直采用图2所示的胎模生产。
关键词 螺栓 反印法 胎模锻造 胎膜制造
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反印法制简易锻模的技术经济分析
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作者 杨建良 《锻压机械》 1993年第5期17-18,共2页
当今,市场竞争激烈,如何降低产品成本已成为十分突出的紧迫问题。在生产中,尽量采用先进可靠的经济合理的工艺方法是进一步降低产品成本的有效途径。为此,我厂在锻件生产,特别是模锻件生产中,试验掌握并广泛应用了用反印法制造简易锻模。
关键词 锻模 制造 反印法
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胎模反印制造存在的缺陷及改进
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作者 郜保国 《铁道机车车辆工人》 1995年第4期11-12,共2页
一般来说,反印法制造胎模模膛,不需复杂的加工设备,整个过程可由锻工自己进行,周期短而实用,制造成本低,模膛表面金属组织致密,纤维连续,锻件易脱模,模具寿命高。因此反印法在国内许多锻造厂都得以广泛应用。但在此法的应用过程中,常存... 一般来说,反印法制造胎模模膛,不需复杂的加工设备,整个过程可由锻工自己进行,周期短而实用,制造成本低,模膛表面金属组织致密,纤维连续,锻件易脱模,模具寿命高。因此反印法在国内许多锻造厂都得以广泛应用。但在此法的应用过程中,常存在以下几方面缺陷。 展开更多
关键词 锻造 胎模锻 反印法 缺陷 改进
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现代金箔印花技术
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作者 岑乐衍 《纺织导报》 CAS 2004年第4期86-88,共3页
金箔印花工艺虽然历史悠久,但发展到今天,无论在所用原料、粘合剂、面料乃至生产设备各方面都有了很大的变化。文章介绍了用辊筒式凹印印花机生产金箔印花(反印法)产品的各个技术环节,并指出金箔印花产品属个性化、小品种产品,市场前景... 金箔印花工艺虽然历史悠久,但发展到今天,无论在所用原料、粘合剂、面料乃至生产设备各方面都有了很大的变化。文章介绍了用辊筒式凹印印花机生产金箔印花(反印法)产品的各个技术环节,并指出金箔印花产品属个性化、小品种产品,市场前景不可低估。 展开更多
关键词 金箔印花 反印法 溶剂型粘合剂 印花机 生产工艺
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超塑成形技术及其制模工艺
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《中国高校技术市场》 2001年第6期48-48,共1页
关键词 超塑成形技术 制模工艺 成果简介 模具 加工方法 反印法原理 技术指标
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Clinical significance of human kallikrein 12 gene expression in gastric cancer 被引量:5
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作者 En-Hao Zhao Zhi-Yong Shen +2 位作者 Hua Liu Xin Jin Hui Cao 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第45期6597-6604,共8页
AIM:To investigate whether the expression of kallikrein 12(KLK12) is related to the development of gastric cancer(GC) and to determine the role of KLK12 in gastric cancer cells growth,invasion and migration.METHODS:Be... AIM:To investigate whether the expression of kallikrein 12(KLK12) is related to the development of gastric cancer(GC) and to determine the role of KLK12 in gastric cancer cells growth,invasion and migration.METHODS:Between September 2007 and March 2008,133 patients with histologically confirmed GC were recruited for the study.Expression of KLK12 was detected in samples from GC patients by quantitative real-time reverse transcription polymerase chain reaction and immunohistochemistry.The relationship between KLK12 protein expression and clinicopathological features of GC was analyzed.The difference in 5-year survival rates between the high KLK12 protein expression group and the low KLK12 expression group was compared.Additionally,the expression of KLK12 was examined in various human GC cell lines,including MKN-28,SGC-7901 and MKN-45.Small interfering RNA(siRNA) was used to inhibit KLK12 expression in MKN-45 cells.Cell clones stably transfected with KLK12 siRNA were tested for KLK12 expression by quantitative real-time reverse transcription-polymerase chain reaction and Western blotting.Furthermore,a series of functional assays were performed in this study to assess the biological features of transfected cells.Cell proliferation was assessed using the methylthiazolyltetrazoliumassay.Finally,cell migration and invasion were assessed using transwell chamber assays.RESULTS:Of the 133 GC patients included in the study,126(94.7%) showed a higher expression level of KLK12 mRNA when compared to noncancerous tissue specimens.Expression of KLK12 mRNA was significantly higher in GC tissues than in normal tissue(P < 0.001).KLK12 protein expression was detected in 96 of 133(72.2%) GC samples with moderate or strong staining primarily in the cytoplasm.In contrast,negative immunostaining for KLK12 protein was observed in the corresponding normal gastric mucosal tissue.Overexpression of KLK12 protein was significantly associated with lymph node metastasis(P = 0.001),histological type(P < 0.001) and tumor-node-metastasis stage(P = 0.005),while no significant correlation was observed between expression of KLK12 protein and sex,age,depth of invasion,tumor size or lymphatic invasion.Furthermore,patients with high KLK12 expression had a significantly poorer 5-year survival rate than those with low KLK12 expression(P = 0.002).Expression of KLK12 mRNA was significantly higher in MKN-45 GC cells compared to normal mucosal cells or two other GC cell lines(P < 0.01).Expression of KLK12 in MKN-45 cells was downregulated after transfection with siRNA.Knockdown of KLK12 markedly decreased the proliferation of MKN-45 cells when compared with parent or mock-transfected cells(P = 0.001),especially from the 3rd to the 5th day of the assay.In migration assays,fewer KLK12 siRNA cells migrated through the chambers(22.00 ± 1.81) when compared to the parent(46.47 ± 2.42) or mock-transfected cells(45.40 ± 1.99);these differences were statistically significant(P < 0.001).However,in the invasion assay,the number of KLK12 siRNA cells that invaded the chambers was 18.40 ± 1.12,closely similar to both the parent(18.67 ± 0.98) and mock-transfected cells(18.53 ± 0.92).There was no significantly difference between the three groups in the invasion assay(P = 0.054).CONCLUSION:The KLK12 gene is markedly overexpressed in GC tissue,and its expression status may be a powerful prognostic indicator for patients with GC.KLK12 might serve as a novel diagnosis and prognosis biomarker in GC. 展开更多
关键词 Gastric cancer Human kallikrein 12 IMMUNO-HISTOCHEMISTRY Prognosis Small interfering RNA Migra-tion INVASION
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