目的建立常山胡柚果皮中3种主要成分的含量测定方法。方法采用RP-HPLC法测定含常山胡柚果皮中芸香柚皮苷、柚皮苷和新橙皮苷的含量,色谱柱为安捷伦ZORBAX Eclipse Plus C_(18)(4.6×250mm,5μm),流动相为甲醇(A)-0.1%乙酸-水溶液(B)...目的建立常山胡柚果皮中3种主要成分的含量测定方法。方法采用RP-HPLC法测定含常山胡柚果皮中芸香柚皮苷、柚皮苷和新橙皮苷的含量,色谱柱为安捷伦ZORBAX Eclipse Plus C_(18)(4.6×250mm,5μm),流动相为甲醇(A)-0.1%乙酸-水溶液(B),流速为1.0mL·min^(-1),于283nm波长下检测,柱温35℃,进样量5μL。结果常山胡柚果皮中芸香柚皮苷、柚皮苷和新橙皮苷在一定范围内线性良好(r>0.9992)。结论该法操作简便,灵敏度高,检测快速,适用于常山胡柚果皮中黄酮类成分的含量测定。展开更多
In the present study,we aimed to investigate the pharmacokinetics and dosage proportionality for a single,intravenous utilization of Pazufloxacin Mesilate Sodium Chloride,an injectable synthetic fluoroquinolone antiba...In the present study,we aimed to investigate the pharmacokinetics and dosage proportionality for a single,intravenous utilization of Pazufloxacin Mesilate Sodium Chloride,an injectable synthetic fluoroquinolone antibacterial agent,in healthy Chinese volunteers.In this open-labeled,three-dosage parallel study,subjects were randomized to receive a single dose of Pazufloxacin Mesilate at 150,300 or 600 mg(n=10,10 and 10,respectively)administered as a 30-min intravenous infusion.Blood and urine samples were serially collected from 0 to 24 h after drug administration.Moreover,the sample’s drug concentrations were analyzed via validated RP-HPLC method.Subjects receiving a single dose of Pazufloxacin Mesilate 150,300 or 600 mg were in accordance with the two compartment model.The Cmax for each dosage group was 2.37±0.89,4.27±0.74 and 10.74±4.06 mg·mL(–1),respectively;and the AUC0→∞was 3.24±1.2,5.89±1.51 and 13.32±2.35 mg·h·mL–1,respectively.In addition,Tmax for groups treated with 150,300 and 600 mg was 0.48±0.08,0.50±0.00 and 0.53±0.08 h,respectively.The correlation analysis for AUC0→∞,Cmax and dosage suggested that pazufolxacin mesilate displayed dose proportion at the dose ranging from 150 to 600 mg.The data suggested that all three different dosage regimens fit with the two compartment model.Meanwhile,it presented a linear correlation between AUC0→∞,Cmax and dosage over the range of 150–600 mg.展开更多
文摘目的建立常山胡柚果皮中3种主要成分的含量测定方法。方法采用RP-HPLC法测定含常山胡柚果皮中芸香柚皮苷、柚皮苷和新橙皮苷的含量,色谱柱为安捷伦ZORBAX Eclipse Plus C_(18)(4.6×250mm,5μm),流动相为甲醇(A)-0.1%乙酸-水溶液(B),流速为1.0mL·min^(-1),于283nm波长下检测,柱温35℃,进样量5μL。结果常山胡柚果皮中芸香柚皮苷、柚皮苷和新橙皮苷在一定范围内线性良好(r>0.9992)。结论该法操作简便,灵敏度高,检测快速,适用于常山胡柚果皮中黄酮类成分的含量测定。
基金the support of Laboratory of National Institution of Drug Clinical Trial,Xiangya Hospital,Central South University
文摘In the present study,we aimed to investigate the pharmacokinetics and dosage proportionality for a single,intravenous utilization of Pazufloxacin Mesilate Sodium Chloride,an injectable synthetic fluoroquinolone antibacterial agent,in healthy Chinese volunteers.In this open-labeled,three-dosage parallel study,subjects were randomized to receive a single dose of Pazufloxacin Mesilate at 150,300 or 600 mg(n=10,10 and 10,respectively)administered as a 30-min intravenous infusion.Blood and urine samples were serially collected from 0 to 24 h after drug administration.Moreover,the sample’s drug concentrations were analyzed via validated RP-HPLC method.Subjects receiving a single dose of Pazufloxacin Mesilate 150,300 or 600 mg were in accordance with the two compartment model.The Cmax for each dosage group was 2.37±0.89,4.27±0.74 and 10.74±4.06 mg·mL(–1),respectively;and the AUC0→∞was 3.24±1.2,5.89±1.51 and 13.32±2.35 mg·h·mL–1,respectively.In addition,Tmax for groups treated with 150,300 and 600 mg was 0.48±0.08,0.50±0.00 and 0.53±0.08 h,respectively.The correlation analysis for AUC0→∞,Cmax and dosage suggested that pazufolxacin mesilate displayed dose proportion at the dose ranging from 150 to 600 mg.The data suggested that all three different dosage regimens fit with the two compartment model.Meanwhile,it presented a linear correlation between AUC0→∞,Cmax and dosage over the range of 150–600 mg.
