Three new nicotinamide adenine dinucleotide(NAD) analogs were synthesized,and their characteristics as cofactors for Escherichia coli malic enzyme(ME) and its double mutant ME L310R/Q401C were analyzed.Each pair of th...Three new nicotinamide adenine dinucleotide(NAD) analogs were synthesized,and their characteristics as cofactors for Escherichia coli malic enzyme(ME) and its double mutant ME L310R/Q401C were analyzed.Each pair of the NAD analog and the double mutant showed good orthogonality to the natural pair of NAD and ME in terms of catalyzing oxidative decarboxylation of L-malic acid.Results indicated that molecular interactions between redox enzyme and cofactor could be further explored to generate new bioorthogonal redox systems.展开更多
In the structural design of tall buildings, peak factors have been widely used to predict mean extreme responses of tall buildings under wind excitations. Vanmarcke＇s peak factor is directly related to an explicit me...In the structural design of tall buildings, peak factors have been widely used to predict mean extreme responses of tall buildings under wind excitations. Vanmarcke＇s peak factor is directly related to an explicit measure of structural reliability against a Gaussian response process. We review the use of this factor for time-variant reliability design by comparing it to the conven- tional Davenport＇s peak factor. Based on the asymptotic theory of statistical extremes, a new closed-form peak factor, the so-called Gamma peak factor, can be obtained for a non-Gaussian resultant response characterized by a Rayleigh distribution process. Using the Gamma peak factor, a combined peak factor method was developed for predicting the expected maximum resultant responses of a building undergoing lateral-torsional vibration. The effects of the standard deviation ratio of two sway components and the inter-component correlation on the evaluation of peak resultant response were also investigated. Utilizing wind tunnel data derived from synchronous multi-pressure measurements, we carried out a wind-induced time history response analysis of the Common- wealth Advisory Aeronautical Research Council （CAARC） standard tall building to validate the applicability of the Gamma peak factor to the prediction of the peak resultant acceleration. Results from the building example indicated that the use of the Gamma peak factor enables accurate predictions to be made of the mean extreme resultant acceleration responses for dynamic service- ability performance design of modem tall buildings.展开更多
基金supported by the National Basic Research Program of China (2012CB721103)the National Natural Science Foundation of China (21102143)
文摘Three new nicotinamide adenine dinucleotide(NAD) analogs were synthesized,and their characteristics as cofactors for Escherichia coli malic enzyme(ME) and its double mutant ME L310R/Q401C were analyzed.Each pair of the NAD analog and the double mutant showed good orthogonality to the natural pair of NAD and ME in terms of catalyzing oxidative decarboxylation of L-malic acid.Results indicated that molecular interactions between redox enzyme and cofactor could be further explored to generate new bioorthogonal redox systems.
基金Project supported by the National Natural Science Foundation of China (No. 51008275)the China Postdoctoral Science Foundation (No.201104736)
文摘In the structural design of tall buildings, peak factors have been widely used to predict mean extreme responses of tall buildings under wind excitations. Vanmarcke＇s peak factor is directly related to an explicit measure of structural reliability against a Gaussian response process. We review the use of this factor for time-variant reliability design by comparing it to the conven- tional Davenport＇s peak factor. Based on the asymptotic theory of statistical extremes, a new closed-form peak factor, the so-called Gamma peak factor, can be obtained for a non-Gaussian resultant response characterized by a Rayleigh distribution process. Using the Gamma peak factor, a combined peak factor method was developed for predicting the expected maximum resultant responses of a building undergoing lateral-torsional vibration. The effects of the standard deviation ratio of two sway components and the inter-component correlation on the evaluation of peak resultant response were also investigated. Utilizing wind tunnel data derived from synchronous multi-pressure measurements, we carried out a wind-induced time history response analysis of the Common- wealth Advisory Aeronautical Research Council （CAARC） standard tall building to validate the applicability of the Gamma peak factor to the prediction of the peak resultant acceleration. Results from the building example indicated that the use of the Gamma peak factor enables accurate predictions to be made of the mean extreme resultant acceleration responses for dynamic service- ability performance design of modem tall buildings.
