Objective:To explore the mechanism of An-pressing manipulation in improving post-stroke muscle spasticity,by observing the changes of γ-aminobutyric acid(GABA)and glycine(GIy)in plasma and gray matter of L1-L3 spinal...Objective:To explore the mechanism of An-pressing manipulation in improving post-stroke muscle spasticity,by observing the changes of γ-aminobutyric acid(GABA)and glycine(GIy)in plasma and gray matter of L1-L3 spinal cord anterior horn in post-stroke rats with muscle spasticity after An-pressing manipulation intervention.Methods:Ten of 80 adult male Sprague-Dawley(SD)rats were randomly selected as the blank group,and the remaining 70 were used for modeling.The middle cerebral artery occlusion(MCAO)rat model was established by insertion suture occlusion method in the left external carotid artery.Thirty rats with a Longa neurological score of 2-3 points and a modified Ashworth spasticity scale score of 1,1+,or 2 were included in the experiment.Using the random number table method,the 30 successfully modeled rats were randomly divided into a model group,an An-pressing tendon group and an An-pressing muscle belly group.Two days after modeling,rats in the An-pressing tendon group and An-pressing muscle belly group received An-pressing manipulation on the tendon and belly of quadriceps femoris muscle respectively,with the pressure of(350±50)g and the frequency of 5 s/time,15 min per session,once a day for 5 continuous days.After the 5th treatment,the tension of the rat quadriceps femoris muscle was evaluated using the modified Ashworth spasticity scale.The Gly levels in rat plasma and L1-L3 segments of spinal cord were determined by enzyme-linked immunosorbent assay(ELISA).The GABA levels in rat plasma and L1-L3 segments of spinal cord were measured by high performance liquid chromatography(HPLC).Results:The decrease in rat muscle tension scored by the modified Ashworth spasticity scale in the An-pressing tendon group was more significant than that in the An-pressing muscle belly group(P<0.01);the increases in Gly and GABA levels in the rat plasma and L1-L3Segments of spinal cord were more significant in the An-pressing tendon group than those in the An-pressing muscle belly group(all P<0.01).展开更多
文摘Objective:To explore the mechanism of An-pressing manipulation in improving post-stroke muscle spasticity,by observing the changes of γ-aminobutyric acid(GABA)and glycine(GIy)in plasma and gray matter of L1-L3 spinal cord anterior horn in post-stroke rats with muscle spasticity after An-pressing manipulation intervention.Methods:Ten of 80 adult male Sprague-Dawley(SD)rats were randomly selected as the blank group,and the remaining 70 were used for modeling.The middle cerebral artery occlusion(MCAO)rat model was established by insertion suture occlusion method in the left external carotid artery.Thirty rats with a Longa neurological score of 2-3 points and a modified Ashworth spasticity scale score of 1,1+,or 2 were included in the experiment.Using the random number table method,the 30 successfully modeled rats were randomly divided into a model group,an An-pressing tendon group and an An-pressing muscle belly group.Two days after modeling,rats in the An-pressing tendon group and An-pressing muscle belly group received An-pressing manipulation on the tendon and belly of quadriceps femoris muscle respectively,with the pressure of(350±50)g and the frequency of 5 s/time,15 min per session,once a day for 5 continuous days.After the 5th treatment,the tension of the rat quadriceps femoris muscle was evaluated using the modified Ashworth spasticity scale.The Gly levels in rat plasma and L1-L3 segments of spinal cord were determined by enzyme-linked immunosorbent assay(ELISA).The GABA levels in rat plasma and L1-L3 segments of spinal cord were measured by high performance liquid chromatography(HPLC).Results:The decrease in rat muscle tension scored by the modified Ashworth spasticity scale in the An-pressing tendon group was more significant than that in the An-pressing muscle belly group(P<0.01);the increases in Gly and GABA levels in the rat plasma and L1-L3Segments of spinal cord were more significant in the An-pressing tendon group than those in the An-pressing muscle belly group(all P<0.01).
