目的探讨戊四氮(PTZ)诱发慢性癫痫大鼠学习记忆能力改变及环磷腺苷反应单元结合蛋白(CREB)表达的变化。方法将20只大鼠随机分为正常对照组和观察组,每组10只,观察组按3.5 m L·kg-1连续腹腔注射10 g·L-1的PTZ 30 d建立慢性癫...目的探讨戊四氮(PTZ)诱发慢性癫痫大鼠学习记忆能力改变及环磷腺苷反应单元结合蛋白(CREB)表达的变化。方法将20只大鼠随机分为正常对照组和观察组,每组10只,观察组按3.5 m L·kg-1连续腹腔注射10 g·L-1的PTZ 30 d建立慢性癫痫模型,以出现全身强直-阵挛性发作为模型制备成功;正常对照组每日腹腔注射生理盐水3.5 m L·kg-1,连续30 d。2组大鼠进行Y迷宫实验,记录错误反应次数(EN)、全天总反应时间(TRT),观察各组大鼠学习记忆能力;Western blot方法测定大鼠海马组织内磷酸化CREB(P-CREB)133位的丝氨酸残基(Ser133)蛋白表达水平。结果观察组大鼠的EN显著高于正常对照组,观察组大鼠的TRT显著长于正常对照组,差异均有统计学意义(P<0.05)。观察组大鼠海马组织中P-CREB(Ser133)蛋白表达水平较正常对照组显著降低,差异有统计学意义(P<0.05)。结论海马组织内CREB表达水平降低可能是癫痫大鼠学习记忆等认知功能障碍发生的分子机制之一。展开更多
One-carbon unit transfer reaction of imidazolinium with 1,2-diaminobenzene has been studied with semi-empirical molecular orbital calculations, PM3. The result shows that there are two ways to complete this reaction b...One-carbon unit transfer reaction of imidazolinium with 1,2-diaminobenzene has been studied with semi-empirical molecular orbital calculations, PM3. The result shows that there are two ways to complete this reaction because the imidazolinium ring has two different breaking patterns. These two ways both have seven steps, including two proton-migrating steps that limit the rate of the reaction. The structures and energies of the intermediates and transition states have been calculated.展开更多
文摘目的探讨戊四氮(PTZ)诱发慢性癫痫大鼠学习记忆能力改变及环磷腺苷反应单元结合蛋白(CREB)表达的变化。方法将20只大鼠随机分为正常对照组和观察组,每组10只,观察组按3.5 m L·kg-1连续腹腔注射10 g·L-1的PTZ 30 d建立慢性癫痫模型,以出现全身强直-阵挛性发作为模型制备成功;正常对照组每日腹腔注射生理盐水3.5 m L·kg-1,连续30 d。2组大鼠进行Y迷宫实验,记录错误反应次数(EN)、全天总反应时间(TRT),观察各组大鼠学习记忆能力;Western blot方法测定大鼠海马组织内磷酸化CREB(P-CREB)133位的丝氨酸残基(Ser133)蛋白表达水平。结果观察组大鼠的EN显著高于正常对照组,观察组大鼠的TRT显著长于正常对照组,差异均有统计学意义(P<0.05)。观察组大鼠海马组织中P-CREB(Ser133)蛋白表达水平较正常对照组显著降低,差异有统计学意义(P<0.05)。结论海马组织内CREB表达水平降低可能是癫痫大鼠学习记忆等认知功能障碍发生的分子机制之一。
文摘One-carbon unit transfer reaction of imidazolinium with 1,2-diaminobenzene has been studied with semi-empirical molecular orbital calculations, PM3. The result shows that there are two ways to complete this reaction because the imidazolinium ring has two different breaking patterns. These two ways both have seven steps, including two proton-migrating steps that limit the rate of the reaction. The structures and energies of the intermediates and transition states have been calculated.
