In order to deal with the dynamic positioning system control problems of dredgers working under strong dredging reaction or harsh environments,an adaptive backstepping method is proposed.Disturbances are estimated and...In order to deal with the dynamic positioning system control problems of dredgers working under strong dredging reaction or harsh environments,an adaptive backstepping method is proposed.Disturbances are estimated and compensated for by the adaptive method without extra sensors on dredging equipment,and the control mechanism is simplified.Adaptive control is used to compensate for the reaction and environmental disturbances on the dredger,so the dredger can maintain the desired position with a minimum error and shock.The proposed adaptive robust controller guarantees the global asymptotic stability of the closed-loop system and rapid position tracking of the dredger.The simulation results show that the proposed controller has superior performance in position tracking and robustness to large disturbances.展开更多
Macroporous methyl acrylate-divinyl benzene copolymers were synthesized and modified by polyethylene polyamine.The aminoacylase from Aspergillus oryzae was adsorded on these modified copolymers.The role of certain fac...Macroporous methyl acrylate-divinyl benzene copolymers were synthesized and modified by polyethylene polyamine.The aminoacylase from Aspergillus oryzae was adsorded on these modified copolymers.The role of certain factors such as degree of cross-linking,amount of poregenic solvent,modifying agent and pore structures,in the activity of immobilized aminoacylase was studied.Effects of adsorption time and amount of enzyme used on the activity of aminocylase immobilized on MMD-12-13 were also investigated.The column of aminoacylase immobilized on MMD-12-13 was prepared and N-acyl-DL-methionine was resolved continuously for a month,the remaining activity was still over 90%.展开更多
AIM: To establish methods for quantitative polymerase chain reaction (PCR) for hepcidin using RNAs isolated from paraffin-embedded sections and in situ hybridization of hepatocellular carcinoma (HCC). METHODS: Total R...AIM: To establish methods for quantitative polymerase chain reaction (PCR) for hepcidin using RNAs isolated from paraffin-embedded sections and in situ hybridization of hepatocellular carcinoma (HCC). METHODS: Total RNA from paraffin-embedded sections was isolated from 68 paraffin-embedded samples of HCC. Samples came from 54 male and 14 female patients with a mean age of 66.8 ± 7.8 years. Quantitative PCR was performed. Immunohistochemistry and in situ hybridization for hepcidin were also performed. RESULTS: Quantitative PCR for hepcidin using RNAs isolated from paraffin-embedded sections of HCC was performed successfully. The expression level of hepcidin mRNA in cancer tissues was significantly higher than that in non-cancer tissues. A method of in situ hybridization for hepcidin was established successfully, and this demonstrated that hepcidin mRNA was expressed in non-cancerous tissue but absent in cancerous tissue. CONCLUSION: We have established novel methods for quantitative PCR for hepcidin using RNAs isolated from paraffin-embedded sections and in situ hybridization of HCC.展开更多
Bioreactor operation requires continuous monitoring of fermentation parameters and real-time control over bioreactor devices. Remote monitoring and control of the bioreactor＇s computer via the Internet avoids the nec...Bioreactor operation requires continuous monitoring of fermentation parameters and real-time control over bioreactor devices. Remote monitoring and control of the bioreactor＇s computer via the Internet avoids the necessity of personnel being continually onsite during operation. A two liter Sartorius-stedim Biostat~ A Plus fermentation system was networked and interfaced with the commercial software from GoToMyPC to allow remote control of the fermentation system utilizing the internet. The fermentation vessel was equipped with hardware calibrated for monitoring and controlling culture parameters during experimentations. The uniform resource locator controlled night-vision web camera allowed continuous monitoring of the glass fermentation vessel during the day and at night. The main window screen of the laboratory computer can be securely accessed from any portable device （i.e. laptop） capable of establishing an Internet connection and executing the commercial software from GoToMyPC. The secured internet protocol address, provided by GoToMyPC, assures that the system can only be controlled by authorized users who have been given access to the account. This interface permits the remote control of the Biostat A Plus fermentation system and possibly other automated or potentially automated culturing systems at the convenience of the user（s）.展开更多
Dear Editor,Hepatitis C virus（HCV）is a positive-strand RNA virus that belongs to the genus Hepacivirus within the Flaviviridae family.HCV causes chronic liver diseases,and185 million people are infected（Messina et ...Dear Editor,Hepatitis C virus（HCV）is a positive-strand RNA virus that belongs to the genus Hepacivirus within the Flaviviridae family.HCV causes chronic liver diseases,and185 million people are infected（Messina et al.,2015）.Currently,there is no approved vaccine to prevent hepatitis C.HCV induces autophagy through elevating reactive oxygen species（ROS）levels via the unfolded protein response （UPR） or via direct interference with the autophagic pathway.展开更多
基金The National Basic Research Program of China (973 Program) (No. 2005CB221505)Open Fund of Provincial Open Laboratory for Control Engineering Key Disciplines (No. KG2009-02)
文摘In order to deal with the dynamic positioning system control problems of dredgers working under strong dredging reaction or harsh environments,an adaptive backstepping method is proposed.Disturbances are estimated and compensated for by the adaptive method without extra sensors on dredging equipment,and the control mechanism is simplified.Adaptive control is used to compensate for the reaction and environmental disturbances on the dredger,so the dredger can maintain the desired position with a minimum error and shock.The proposed adaptive robust controller guarantees the global asymptotic stability of the closed-loop system and rapid position tracking of the dredger.The simulation results show that the proposed controller has superior performance in position tracking and robustness to large disturbances.
文摘Macroporous methyl acrylate-divinyl benzene copolymers were synthesized and modified by polyethylene polyamine.The aminoacylase from Aspergillus oryzae was adsorded on these modified copolymers.The role of certain factors such as degree of cross-linking,amount of poregenic solvent,modifying agent and pore structures,in the activity of immobilized aminoacylase was studied.Effects of adsorption time and amount of enzyme used on the activity of aminocylase immobilized on MMD-12-13 were also investigated.The column of aminoacylase immobilized on MMD-12-13 was prepared and N-acyl-DL-methionine was resolved continuously for a month,the remaining activity was still over 90%.
基金Supported by A research grant from the Biomarker Society
文摘AIM: To establish methods for quantitative polymerase chain reaction (PCR) for hepcidin using RNAs isolated from paraffin-embedded sections and in situ hybridization of hepatocellular carcinoma (HCC). METHODS: Total RNA from paraffin-embedded sections was isolated from 68 paraffin-embedded samples of HCC. Samples came from 54 male and 14 female patients with a mean age of 66.8 ± 7.8 years. Quantitative PCR was performed. Immunohistochemistry and in situ hybridization for hepcidin were also performed. RESULTS: Quantitative PCR for hepcidin using RNAs isolated from paraffin-embedded sections of HCC was performed successfully. The expression level of hepcidin mRNA in cancer tissues was significantly higher than that in non-cancer tissues. A method of in situ hybridization for hepcidin was established successfully, and this demonstrated that hepcidin mRNA was expressed in non-cancerous tissue but absent in cancerous tissue. CONCLUSION: We have established novel methods for quantitative PCR for hepcidin using RNAs isolated from paraffin-embedded sections and in situ hybridization of HCC.
文摘Bioreactor operation requires continuous monitoring of fermentation parameters and real-time control over bioreactor devices. Remote monitoring and control of the bioreactor＇s computer via the Internet avoids the necessity of personnel being continually onsite during operation. A two liter Sartorius-stedim Biostat~ A Plus fermentation system was networked and interfaced with the commercial software from GoToMyPC to allow remote control of the fermentation system utilizing the internet. The fermentation vessel was equipped with hardware calibrated for monitoring and controlling culture parameters during experimentations. The uniform resource locator controlled night-vision web camera allowed continuous monitoring of the glass fermentation vessel during the day and at night. The main window screen of the laboratory computer can be securely accessed from any portable device （i.e. laptop） capable of establishing an Internet connection and executing the commercial software from GoToMyPC. The secured internet protocol address, provided by GoToMyPC, assures that the system can only be controlled by authorized users who have been given access to the account. This interface permits the remote control of the Biostat A Plus fermentation system and possibly other automated or potentially automated culturing systems at the convenience of the user（s）.
基金supported by grants from the National Natural Science Foundation of China(81471955,81672035,81301438)the CAMS Innovation Fund for Medical Sciences(CIFMS)(2016-I2M-3-020)+2 种基金the National Key Plan for Research and Development of China(2016YFD0500300)the Program for Changjiang Scholars and Innovative Research Team in University(IRT13007)the Fundamental Research Funds for the Central Universities,the PUMC Youth Fund(3332016085)
文摘Dear Editor,Hepatitis C virus（HCV）is a positive-strand RNA virus that belongs to the genus Hepacivirus within the Flaviviridae family.HCV causes chronic liver diseases,and185 million people are infected（Messina et al.,2015）.Currently,there is no approved vaccine to prevent hepatitis C.HCV induces autophagy through elevating reactive oxygen species（ROS）levels via the unfolded protein response （UPR） or via direct interference with the autophagic pathway.
