目的：探讨切除上唇系带及周围痔疮反应结节内服麻仁滋脾治疗痔疮的临床效果。方法将300例痔疮患者按随机数字表法分为2组，每组150例，两组运用祖国医学整体观念思想的指导下，以肛门镜检查“痔”反应点，口腔镜检查上唇系带“痔”反...目的：探讨切除上唇系带及周围痔疮反应结节内服麻仁滋脾治疗痔疮的临床效果。方法将300例痔疮患者按随机数字表法分为2组，每组150例，两组运用祖国医学整体观念思想的指导下，以肛门镜检查“痔”反应点，口腔镜检查上唇系带“痔”反应点，确认“痔”反应点，分别进行手术治疗。A 组患者采用单纯的切除痔疮反应结节手术治疗；B 组患者给予上唇系带及周围痔疮反应结节，同时服用麻仁滋脾加减进行治疗，比较两组疗效。结果 B 组痔疮患者治疗后临床治愈率[92．29％（138／150）]及总有效率[96．0％（144／150）]显著高于 A 组[76．67％（115／150），85．33％（128／150），χ2＝10．08，10．60，P ＜0．01]。结论切除上唇系带痔疮反应结节及周围组织联合口服用麻仁滋脾加减治疗，其效果好，内服麻仁滋脾，润肠通便，减少局部刺激以利痔核消散吸收。本治疗方法简便易操作，实用、费用低廉，值得临床推广。展开更多
Objective To explore the role of the extracellular signal-regulated kinase （ERK）/cAMP response element binding protein （CREB） pathway in the induction of long-term potentiation （LTP） in the anterior cingulate co...Objective To explore the role of the extracellular signal-regulated kinase （ERK）/cAMP response element binding protein （CREB） pathway in the induction of long-term potentiation （LTP） in the anterior cingulate cortex （ACC） that may be implicated in pain-related negative emotion. Methods LTP of field potential was recorded in ACC slice and the expressions of phospho-ERK （pERK） and phospho-CREB （pCREB） were examined using immunohistochemistry method. Results LTP could be induced stably in ACC slice by high frequency stimulation （2-train, 100 Hz, 1 s）, while APv （an antagonist of NMDA receptor） could block the induction of LTP in the ACC, indicating that LTP in this experiment was NMDA receptor-dependent. Bath application of PD98059 （50 μmol/L）, a selective MEK inhibitor, at 30 min before tetanic stimulation could completely block the induction of LTP. Moreover, the protein level of pERK in the ACC was transiently increased after LTP induction, starting at 5 rain and returning to basal at 1 h after tetanic stimulation. The protein level of pCREB was also increased after LTP induction. The up-regulation in pERK and pCREB expressions could be blocked by pretreatment of PD98059. Double immunostaining showed that after LTP induction, most pERK was co-localized with pCREB. Conclusion NMDA receptor and ERK-CREB pathway are necessary for the induction of LTP in rat ACC and may play important roles in pain emotion.展开更多
文摘目的：探讨切除上唇系带及周围痔疮反应结节内服麻仁滋脾治疗痔疮的临床效果。方法将300例痔疮患者按随机数字表法分为2组，每组150例，两组运用祖国医学整体观念思想的指导下，以肛门镜检查“痔”反应点，口腔镜检查上唇系带“痔”反应点，确认“痔”反应点，分别进行手术治疗。A 组患者采用单纯的切除痔疮反应结节手术治疗；B 组患者给予上唇系带及周围痔疮反应结节，同时服用麻仁滋脾加减进行治疗，比较两组疗效。结果 B 组痔疮患者治疗后临床治愈率[92．29％（138／150）]及总有效率[96．0％（144／150）]显著高于 A 组[76．67％（115／150），85．33％（128／150），χ2＝10．08，10．60，P ＜0．01]。结论切除上唇系带痔疮反应结节及周围组织联合口服用麻仁滋脾加减治疗，其效果好，内服麻仁滋脾，润肠通便，减少局部刺激以利痔核消散吸收。本治疗方法简便易操作，实用、费用低廉，值得临床推广。
基金supported by National Natural Science Fundation of China (No.30870835,30821002,and 30900444)National Basic Research Program of China (No. 2007CB512303,2007CB512502,and 2006CB500807)Postdoctoral Fundation of China (No.20080440578)
文摘Objective To explore the role of the extracellular signal-regulated kinase （ERK）/cAMP response element binding protein （CREB） pathway in the induction of long-term potentiation （LTP） in the anterior cingulate cortex （ACC） that may be implicated in pain-related negative emotion. Methods LTP of field potential was recorded in ACC slice and the expressions of phospho-ERK （pERK） and phospho-CREB （pCREB） were examined using immunohistochemistry method. Results LTP could be induced stably in ACC slice by high frequency stimulation （2-train, 100 Hz, 1 s）, while APv （an antagonist of NMDA receptor） could block the induction of LTP in the ACC, indicating that LTP in this experiment was NMDA receptor-dependent. Bath application of PD98059 （50 μmol/L）, a selective MEK inhibitor, at 30 min before tetanic stimulation could completely block the induction of LTP. Moreover, the protein level of pERK in the ACC was transiently increased after LTP induction, starting at 5 rain and returning to basal at 1 h after tetanic stimulation. The protein level of pCREB was also increased after LTP induction. The up-regulation in pERK and pCREB expressions could be blocked by pretreatment of PD98059. Double immunostaining showed that after LTP induction, most pERK was co-localized with pCREB. Conclusion NMDA receptor and ERK-CREB pathway are necessary for the induction of LTP in rat ACC and may play important roles in pain emotion.
