A method about the isolation of heterotrophic nitrosobactria and the characteristics of heterotrophic nitrosobactria were studied.It can be seen from the fluorescence in situ hybridization results of the bio-membrane ...A method about the isolation of heterotrophic nitrosobactria and the characteristics of heterotrophic nitrosobactria were studied.It can be seen from the fluorescence in situ hybridization results of the bio-membrane sample from the bio-ceramic reactor,the spots of green nitrosobacteria are obviously more than those of orange nitrobacteria.Two heterotrophic nitrobacteria were isolated from the bio-ceramic reactor.By sequencing 16SrDNA and establishing the phylogenic tree,they were identified physiologically and biochemically as Pseudomonas sp..After 12 d,the COD removal efficiency of wgy55 and wgy68 were 45.03% and 50.85%,the NH4-N removal efficiency of them were 80.12% and 85.93%,and the TN removal efficiency of them were 69.71% and 64.7%.The final concentration of NO2-N of wgy55 and wgy68 was 0.753 mg/L and 0.601 mg/L,and that of NO3-N was 3.21 mg/L and 3.38 mg/L.These results show that wgy55 and wgy68 have the capability of nitrification and they are heterotrophic nitrobacteria.展开更多
基金Sponsored by the National Natural Science Foundation of China(Grant No.50521140075)
文摘A method about the isolation of heterotrophic nitrosobactria and the characteristics of heterotrophic nitrosobactria were studied.It can be seen from the fluorescence in situ hybridization results of the bio-membrane sample from the bio-ceramic reactor,the spots of green nitrosobacteria are obviously more than those of orange nitrobacteria.Two heterotrophic nitrobacteria were isolated from the bio-ceramic reactor.By sequencing 16SrDNA and establishing the phylogenic tree,they were identified physiologically and biochemically as Pseudomonas sp..After 12 d,the COD removal efficiency of wgy55 and wgy68 were 45.03% and 50.85%,the NH4-N removal efficiency of them were 80.12% and 85.93%,and the TN removal efficiency of them were 69.71% and 64.7%.The final concentration of NO2-N of wgy55 and wgy68 was 0.753 mg/L and 0.601 mg/L,and that of NO3-N was 3.21 mg/L and 3.38 mg/L.These results show that wgy55 and wgy68 have the capability of nitrification and they are heterotrophic nitrobacteria.
