The effect of magnetic field on a-amylase was studied. Under the experimental conditions, a-amylase solution was treated by 0.15 T, 0.30 T and 0.45 T static magnetic fields for a known period of time, then the activit...The effect of magnetic field on a-amylase was studied. Under the experimental conditions, a-amylase solution was treated by 0.15 T, 0.30 T and 0.45 T static magnetic fields for a known period of time, then the activity, kinetic parameters, and the secondary conformation were investigated. The results showed that there was a considerable effect of the magnetic exposure on the α-amylase. The activity was increased by 27%, 34.1%, 37.8% compared with the control. It was also found that both kinetic parameters Km and Vm could be decreased due to the increasing magnetic field, Km decreased from 2.20×10^2 to 0.87×10^2, whereas Vm decreased from 2.0×10^3 g/min to 1.1 ×10^3 g/min. At the same time, there were some irregular changes in a-amylase secondary conformation.展开更多
基金Supported by Tianjin Natural Science Foundation (No033603611)
文摘The effect of magnetic field on a-amylase was studied. Under the experimental conditions, a-amylase solution was treated by 0.15 T, 0.30 T and 0.45 T static magnetic fields for a known period of time, then the activity, kinetic parameters, and the secondary conformation were investigated. The results showed that there was a considerable effect of the magnetic exposure on the α-amylase. The activity was increased by 27%, 34.1%, 37.8% compared with the control. It was also found that both kinetic parameters Km and Vm could be decreased due to the increasing magnetic field, Km decreased from 2.20×10^2 to 0.87×10^2, whereas Vm decreased from 2.0×10^3 g/min to 1.1 ×10^3 g/min. At the same time, there were some irregular changes in a-amylase secondary conformation.
