Objective: To evaluate the humoral immune induction in rats of a candidate AIDS vaccine expressing the gag p24 gene froma subtype B HIV-1 isolate. Methods: The amplified p24 gene was inserted into aneukaryotic express...Objective: To evaluate the humoral immune induction in rats of a candidate AIDS vaccine expressing the gag p24 gene froma subtype B HIV-1 isolate. Methods: The amplified p24 gene was inserted into aneukaryotic expression vector to form the supercoiled DNAvaccine. The linearized expressed DNA vaccine was preparedfrom the expression plasmid by polymerase chain reaction(PCR). The antigen gene expression in rats of the linearizedand supercoiled DNA vaccines were in vitro and in vivodetected. Results: In vitro transcription and Northern hybridizationshowed that the linearized DNA vaccine could synthesizeamounts or p24 mRNA similar to the supercoiled DNA vaccine.Antibody assays of inoculated rats confirmed that thelinearized expression DNA could induce a slightly higherantibody titer than the expression plasmid, while the highestautibody titer had been induced by plasmid plus adjuvantinoculation. Conclusion: The construction of a candidate AIDS vaccinebased on the p24 gene could shed light on a potential IV vaccine, meriting evaluation in a rhesus macaque SHIV-AIDSmodel.展开更多
A pot culture experiment was carried out for the Screening of 11 popular genotypes against to Meloidogyne incognita and Fusarium chlamydosporum wilt complex on Coleus forskohlii. Genotype viz., yellow tubers and Rabak...A pot culture experiment was carried out for the Screening of 11 popular genotypes against to Meloidogyne incognita and Fusarium chlamydosporum wilt complex on Coleus forskohlii. Genotype viz., yellow tubers and Rabakavi local showed resistant reaction, Orange tuber and Nimbanur local showed moderately resistant reaction. Rest of the seven genotvDes showed suscentible reactions.展开更多
The high resolution 3D nonlinear integrated inversion method is based on nonlinear theory. Under layer control, the log data from several wells （or all wells） in the study area and seismic trace data adjacent to the...The high resolution 3D nonlinear integrated inversion method is based on nonlinear theory. Under layer control, the log data from several wells （or all wells） in the study area and seismic trace data adjacent to the wells are input to a network with multiple inputs and outputs and are integratedly trained to obtain an adaptive weight function of the entire study area. Integrated nonlinear mapping relationships are built and updated by the lateral and vertical geologic variations of the reservoirs. Therefore, the inversion process and its inversion results can be constrained and controlled and a stable seismic inversion section with high resolution with velocity inversion, impedance inversion, and density inversion sections, can be gained. Good geologic effects have been obtained in model computation tests and real data processing, which verified that this method has high precision, good practicality, and can be used for quantitative reservoir analysis.展开更多
基金Financially supported by Natural Science Foundation of China(No 39870725)Natural Science Foundation of Guangdong(No 980642)Research Foundation of Guangdong Education Buresu(No.20032).
文摘Objective: To evaluate the humoral immune induction in rats of a candidate AIDS vaccine expressing the gag p24 gene froma subtype B HIV-1 isolate. Methods: The amplified p24 gene was inserted into aneukaryotic expression vector to form the supercoiled DNAvaccine. The linearized expressed DNA vaccine was preparedfrom the expression plasmid by polymerase chain reaction(PCR). The antigen gene expression in rats of the linearizedand supercoiled DNA vaccines were in vitro and in vivodetected. Results: In vitro transcription and Northern hybridizationshowed that the linearized DNA vaccine could synthesizeamounts or p24 mRNA similar to the supercoiled DNA vaccine.Antibody assays of inoculated rats confirmed that thelinearized expression DNA could induce a slightly higherantibody titer than the expression plasmid, while the highestautibody titer had been induced by plasmid plus adjuvantinoculation. Conclusion: The construction of a candidate AIDS vaccinebased on the p24 gene could shed light on a potential IV vaccine, meriting evaluation in a rhesus macaque SHIV-AIDSmodel.
文摘A pot culture experiment was carried out for the Screening of 11 popular genotypes against to Meloidogyne incognita and Fusarium chlamydosporum wilt complex on Coleus forskohlii. Genotype viz., yellow tubers and Rabakavi local showed resistant reaction, Orange tuber and Nimbanur local showed moderately resistant reaction. Rest of the seven genotvDes showed suscentible reactions.
基金supported by the Key Project of the National Natural Scientific Foundation(Grant No.40839909)
文摘The high resolution 3D nonlinear integrated inversion method is based on nonlinear theory. Under layer control, the log data from several wells （or all wells） in the study area and seismic trace data adjacent to the wells are input to a network with multiple inputs and outputs and are integratedly trained to obtain an adaptive weight function of the entire study area. Integrated nonlinear mapping relationships are built and updated by the lateral and vertical geologic variations of the reservoirs. Therefore, the inversion process and its inversion results can be constrained and controlled and a stable seismic inversion section with high resolution with velocity inversion, impedance inversion, and density inversion sections, can be gained. Good geologic effects have been obtained in model computation tests and real data processing, which verified that this method has high precision, good practicality, and can be used for quantitative reservoir analysis.
