高氯·马乳油的反相液相色谱分析

高氯·马乳油是由高效氯氰菊酯和马拉硫磷复配而成的广谱、高效的杀虫剂。本文采用反相液相色谱外标法,以甲醇和水作流动相对该产品进行定量分析,方法操作简便,精密度、准确度均符合分析要求。

氟草烟的反相高效液相色谱分析

文章报道了除草剂氟草烟的反相高效液相色谱分析方法.选用以ODS为填料的250mm×4.6mm(id)不锈钢柱,以邻苯二甲酸二甲酯为内标物,进行定量分析测定,氟草烟的线性回归系数分别为0.9997,方法回收率在99.43%～100.32%之间,标准偏差小于0.10%,变异系数为0.4%.

添加蛋白酶微胶囊干酪的反相高效液相色谱分析

利用阿拉伯胶和明胶为壁材,以毛霉蛋白酶为芯材,采用冷冻干燥技术制备蛋白酶微胶囊,用于干酪制备,促进其成熟。在干酪成熟过程中采用反相高效液相色谱技术分析添加蛋白酶微胶囊的干酪中水溶性氮提取液,并对色谱峰的分布情况进行主成分分析。试验结果表明:在干酪成熟期间,随着成熟时间的延长,亲水性小分子质量肽含量逐渐增大;亲水性中分子质量肽的含量先增加后降低;大分子质量疏水性肽的含量无太大变化。

HPLC测定冬虫夏草菌粉中腺苷含量

介绍HPLC测定冬虫夏草菌粉中腺苷含量的分析方法。确定苷在在HPLC上的线性关系和最低检出限等。该方法灵敏度高、选择性好,样品前处理方法简单快速,回收率符合试验要求,结果令人满意。

Creation of reversed phase high-performance liquid chromatographic technique to assay platelet-activating factor

Objective: To establish a new assay for platelet-activating factor (PAF), to compare it with bio-assay; and to discuss its significance in some elderly people diseases such as cerebral infarction and coronary heart disease. Methods: To measure PAF levels in 100 controls, 23 elderly patients with cerebral infarction and 65 cases with coronary heart disease by reversed phase high-performance liquid chromatographic technique (rHPLC). Results:rHPLC is more convenient, sensitive,specific, and less confusing, compared with bio-assay. The level of plasma PAF in patients with cerebral infarction was higher than that in the controls (P<0.01), and in patients with coronary heart disease. Conclusion: Detection of PAF with rHPLC is more reliable and more accurate. The new assay has important significance in PAF research.