为了改进现有的组反k最近邻查询算法的查询速度与准确度,提出了一种基于Voronoi图的组反k最近邻查询方法(group reverse k nearest neighbor guery method based on Voronoi diagram,V_GRk NN)。该方法获得的结果集是将这组查询点中任...为了改进现有的组反k最近邻查询算法的查询速度与准确度,提出了一种基于Voronoi图的组反k最近邻查询方法(group reverse k nearest neighbor guery method based on Voronoi diagram,V_GRk NN)。该方法获得的结果集是将这组查询点中任意一点作为kN N的数据点集合,在实际应用中可以用来评估一组查询对象的影响力。该方法的特点是首先对查询点集Q进行优化处理,降低查询点数量对查询效率的负面影响;接着对数据点集P进行约减,缩小查询搜索范围;然后根据基于Voronoi图的剪枝策略对候选集进行过滤;最后经过精炼获得GRk NN查询的结果集。该方法在数据集处理阶段很大程度上提高了查询速度,在过滤、精炼阶段利用Voronoi图的特性提高了查询的准确性。理论研究和实验表明,所提方法的效率明显优于可选的已有方法。展开更多
The interfacial reaction behavior of Al and Ti_3AlC_(2)at different pouring temperatures and its effect on the microstructure and mechanical properties of the composites were investigated.The results show that the add...The interfacial reaction behavior of Al and Ti_3AlC_(2)at different pouring temperatures and its effect on the microstructure and mechanical properties of the composites were investigated.The results show that the addition of3.0 wt.%Ti_3AlC_(2)refines the average grain size ofα(Al)in the composite by 50.1%compared to Al6061 alloy.Morphological analyses indicate that an in-situ Al_(3Ti)transition layer of-180 nm in thickness is generated around the edge of Ti_3AlC_(2)at 720℃,forming a well-bonded Al-Al_(3Ti)interface.At this processing temperature,the ultimate tensile strength of A16061-3.0 wt.%Ti_3AlC_(2)composite is 199.2 MPa,an improvement of 41.5%over the Al6061 matrix.Mechanism analyses further elucidate that 720℃is favourable for forming the nano-sized transition layer at the Ti_3AlC_(2)edges.And,the thermal mismatch strengthening plays a dominant role in this state,with a strengthening contribution of about 74.8%.展开更多
Al-Ti diffusion couples were made by embedded technology and treated at the temperature between the melting points of Al and Ti. The microstructure evolution and growth mechanism of the Al-Ti DRZ were investigated. Th...Al-Ti diffusion couples were made by embedded technology and treated at the temperature between the melting points of Al and Ti. The microstructure evolution and growth mechanism of the Al-Ti DRZ were investigated. The result shows that the DRZ, the mixture of TiAl3 and Al, grows layer by layer along their chemical equilibrium zone. In the course, the growth interface moves toward the aluminum side. TiAl3 is the only new phase which forms earliest in the course of heat-treatment. The growth mechanism of the DRZ changes after the phase transition of titanium. Before the phase transition of titanium, the growth of the DRZ is controlled by the dissolution speed of the titanium to the molten aluminum, while after the phase transition of titanium, the growth is controlled by the chemical reaction speed of Al and Ti atoms, and consequently, its growth rate is greatly increased.展开更多
Contact reactive brazing of 6063 Al alloy and 1Cr18Ni9Ti stainless steel was researched by using Cu as interlayer. Effect of brazing time on microstructure of the joints, as well as the dissolution behaviors of Cu int...Contact reactive brazing of 6063 Al alloy and 1Cr18Ni9Ti stainless steel was researched by using Cu as interlayer. Effect of brazing time on microstructure of the joints, as well as the dissolution behaviors of Cu interlayer was analyzed. The results show that the product of reaction zone near 1Cr18Ni9Ti is composed of Fe2Al5, FeAl3 intermetallic compound (IMC), and Cu-Al IMC; the near by area is composed of Al-Cu eutectic structure with Al (Cu) solid solution. With increasing the brazing time, the thickness of IMC layer at the interface increases, while the width of Al-Cu eutectic structure with Al(Cu) solution decreases. Calculation shows the dissolution rate of Cu interlayer is very fast. The complete dissolution time is about 0.47 s for Cu interlayer with 10 μm in thickness used in this study.展开更多
AZ31-4.6% Mg2Si (mass fraction) composite was prepared by conventional casting method. Repetitive upsetting (RU) was applied to severely deforming the as-cast composite at 400 ℃ for 1, 3, and 5 passes. Finite ele...AZ31-4.6% Mg2Si (mass fraction) composite was prepared by conventional casting method. Repetitive upsetting (RU) was applied to severely deforming the as-cast composite at 400 ℃ for 1, 3, and 5 passes. Finite element analysis of the material flow indicates that deformation concentrates in the bottom region of the sample after 1 pass, and much more uniform deformation is obtained after 5 passes. During multi-pass RU process, both dendritic and Chinese script type Mg2Si phases are broken up into smaller particles owing to the shear stress forced by the matrix. With the increasing number of RU passes, finer grain size and more homogeneous distribution of Mg2Si particles are obtained along with significant enhancement in both strength and ductility. AZ31-4.6%Mg2Si composite exhibits tensile strength of 284 MPa and elongation of 9.8%after 5 RU passes at 400 ℃ compared with the initial 128 MPa and 5.4%of original AZ31-4.6%Mg2Si composite.展开更多
The delay-dependent absolute stability for a class of Lurie systems with interval time-varying delay is studied. By employing an augmented Lyapunov functional and combining a free-weighting matrix approach and the rec...The delay-dependent absolute stability for a class of Lurie systems with interval time-varying delay is studied. By employing an augmented Lyapunov functional and combining a free-weighting matrix approach and the reciprocal convex technique, an improved stability condition is derived in terms of linear matrix inequalities (LMIs). By retaining some useful terms that are usually ignored in the derivative of the Lyapunov function, the proposed sufficient condition depends not only on the lower and upper bounds of both the delay and its derivative, but it also depends on their differences, which has wider application fields than those of present results. Moreover, a new type of equality expression is developed to handle the sector bounds of the nonlinear function, which achieves fewer LMIs in the derived condition, compared with those based on the convex representation. Therefore, the proposed method is less conservative than the existing ones. Simulation examples are given to demonstrate the validity of the approach.展开更多
Equilibrium freezing curve of Mg-2%Ga (mass fraction) alloy was calculated by CALPHALD method. Microstructures of the melted Mg-2%Ga alloys solidified by iron and copper moulds, respectively, were investigated using...Equilibrium freezing curve of Mg-2%Ga (mass fraction) alloy was calculated by CALPHALD method. Microstructures of the melted Mg-2%Ga alloys solidified by iron and copper moulds, respectively, were investigated using OM and SEM. Electrochemical properties of the Mg-2%Ga alloys with different freezing rates were measured by galvanostatic, potentiodynamic and electrochemical impedance spectroscopy tests. The results show that solidification by copper mould leads to intergranular MgsGa2 compounds with small size and large number density. Less adsorbent of Mg^+ and oxide corrosion products occur on the surface of the Mg-2%Ga alloys solidified by copper mould, producing lower corrosion current density of 1.8×10^-5 mA/cm^2. In the galvanostatic tests with 100 mA/cm^2 current density, more negative stable potential of-1.604 V exists in the Mg-2%Ga alloys solidified by iron mould due to the lower freezing rate, which leads to smaller inductive and capacity time constants as well as shorter activity time and better electrochemical activity.展开更多
Objective: To construct recombinant retroviral vector containing HIV-1 Tatgene and evaluate the junction of the expressed Tat in target cells. Methods: HIV-1 Tat_(101) genewas recovered from pEV plasmid by Hind Ⅲ dig...Objective: To construct recombinant retroviral vector containing HIV-1 Tatgene and evaluate the junction of the expressed Tat in target cells. Methods: HIV-1 Tat_(101) genewas recovered from pEV plasmid by Hind Ⅲ digestion and cloned into expression plasmid LZESpBMN-Z toconstruct recombinant retroviral expression plasmid named LZRS-Tat_(101). Using the method ofcalcium phosphate, the construct of LZRS-Tat_(101) was then transfected into packaging cell linesPhoenix (ΦNX) which contained env and gal genes encoding structural proteins and pol gene codingfor 3 enzymes ( reverse transcriptase, protease and integrate) essential for retroviral integrationand replication . The stable transfected cell lines was obtained using puromycin to screen for morethan 3 days. Then, immunohistochemical (IHC ) staining was carried out to detect the expressionlevel of Tat_(101) protein in both transiently and stably trancfected ΦNX, respectively. Thesupematants containing recombinant virus collected from transient and stable transfected cells wereemployed to infect 293 cells, respectively, and the expressed Tat in 293 cells was tested by Westernblot. Meantime, the supematants of infected 293 cells was further added to HL3T1 cells which wereHela cell lines containing an HIV-1-LTR/CAT reporter construct to establish a co-culture system.After co-culture for 72 hours, the protein was extracted from HL3T1 cells and used for CAT activityassay. Results: After LZRS- Tat_(101) was transfected into ΦNX, the amount of expressed Tat intransient transfection cells was significantly higher than that in stable transfection cells; Tatcould be detected not only in 293 cells but also in the supematants from 293 cells culture, and Tatin the supematants could activate HIV-1 LTR promoter in HL3T1, resulting in high 'expression of CATlocated at the downstream of LTR. Conclusion: The construct of recombinant retrovirus LZRS-Tat_(101) could express Tat protein in target cells and the expressed Tat was functionally activeand can really exhibit the ability to activate transcription.展开更多
A multidisciplinary approach-anatomy, histochemistry and phytochemistry-was used to investigate the leaf structure, the content and the storage location of barbaloin in the leaves of Aloe vera L. var. chinensis (Haw.)...A multidisciplinary approach-anatomy, histochemistry and phytochemistry-was used to investigate the leaf structure, the content and the storage location of barbaloin in the leaves of Aloe vera L. var. chinensis (Haw.) Berg. Xeromorphic characteristics including secondary thickened epidermal cell walls, thicker cuticle, ambiguous differentiation of spongy and palisade tissues in the chlorenchyma, and well-developed aquiferous tissue could be seen in the leaves. Several large parenchymatous cells were observed at the phloem pole of the first ring of vascular bundles. The secondary ring of vascular bundles in the leaf base and the stomata, which are surrounded by five cells, have some classification significance in this species. The density of vascular bundles, the content of barbaloin and the intensity of histochemical reaction differed among leaf numbers Ll (annual leaf), L2 (biennial leaf), L3 (triennial leaf) and L4 (quadrennial leaf), and in different parts of the leaf. These three factors were highest in the youngest leaf, Ll, and top parts of all the leaves and lowest in the basal parts and the oldest leaf, L4. The density of vascular bundles had a positive correlation to the content of barbaloin. The histochemical results revealed that the small sheath cells that surrounded the bundles might be the location of barbaloin synthesis and the large parenchymatous cells beneath the sheath might be the storage places of this metabolite.展开更多
[Objective] This study aimed to explore an effective method for rapid salt- extraction of high-quality genomic DNA from dried seeds of plants. [Method] Seeds of seven varieties of crops were ground into powder. A hund...[Objective] This study aimed to explore an effective method for rapid salt- extraction of high-quality genomic DNA from dried seeds of plants. [Method] Seeds of seven varieties of crops were ground into powder. A hundred milligrams of seed powder was added to extracting solution for high salt-extraction of genomic DNA. The yield and quality of extracted DNA were determined by using ultramicro UV/Vis spectrophotometer detection method, PCR and restriction enzyme digestion. [Result] About 619.67-1 811.21 ng of genomic DNA was extracted from per 100 mg of dried seed powder of seven varieties of conventional crops. A260/A280 ratios of the obtained DNA solution all ranged from 1.87 to 2.07, the purity and quality of PCR were suitable for PCR and restriction enzyme digestion. Clear target bands of specific endogenous gene fragments of seven varieties of crops were amplified by PCR, and the obtained DNA could be fully digested with EcoRV and Hindlll.[Conclusion] This method could be used for rapid extraction of high-quality genomic DNA from dried seeds.展开更多
Chloroplast genetic engineering, with several advantages over nuclear genetic engineering, is now regarded as an attractive new technology in basic and applied research, including deepening our understanding of plasti...Chloroplast genetic engineering, with several advantages over nuclear genetic engineering, is now regarded as an attractive new technology in basic and applied research, including deepening our understanding of plastid genome, engineering plant metabolic system, generating transplastomic plants with higher resistance to insect, disease, drought and herbicide and bioproducing of antibodies and vaccines. In this review, the principle and operating system for chloroplast genetic engineering and its application in higher plants have been discussed.展开更多
(ZrB2+Al2O3+Al3Zr)/A356 composites were synthesized by melt direct reaction from A356-(K2ZrF6+KBF4+Na2B4O7) system.The phase compositions and the microstructures of the as-prepared composites were investigated...(ZrB2+Al2O3+Al3Zr)/A356 composites were synthesized by melt direct reaction from A356-(K2ZrF6+KBF4+Na2B4O7) system.The phase compositions and the microstructures of the as-prepared composites were investigated by XRD,SEM and TEM.The results show that the reinforcements are composed of ZrB2 and Al2O3 ceramic phase particles and Al3Zr intermetallic particles.The ZrB2 particulates are easy to join together to form some particle clusters and distribute along the α(Al) grain boundary.The morphologies of the ZrB2 particulates are in hexagon-shape with the size of about 50 nm.The TEM investigation results of Al3Zr indicate that Al3Zr grows in the form of facet with the length-diameter ratio of about 20.The morphologies of Al2O3 particles are in rectangular-shape and ellipsoidal-shape,with the size of about 0.1 μm.In addition,the interfaces of the matrix and particles are net and no interfacial outgrowth is observed.展开更多
文摘为了改进现有的组反k最近邻查询算法的查询速度与准确度,提出了一种基于Voronoi图的组反k最近邻查询方法(group reverse k nearest neighbor guery method based on Voronoi diagram,V_GRk NN)。该方法获得的结果集是将这组查询点中任意一点作为kN N的数据点集合,在实际应用中可以用来评估一组查询对象的影响力。该方法的特点是首先对查询点集Q进行优化处理,降低查询点数量对查询效率的负面影响;接着对数据点集P进行约减,缩小查询搜索范围;然后根据基于Voronoi图的剪枝策略对候选集进行过滤;最后经过精炼获得GRk NN查询的结果集。该方法在数据集处理阶段很大程度上提高了查询速度,在过滤、精炼阶段利用Voronoi图的特性提高了查询的准确性。理论研究和实验表明,所提方法的效率明显优于可选的已有方法。
基金financially supported by the National Natural Science Foundation of China(No.51965040)Science and Technology Project of Jiangxi Provincial Department of Transportation,China(No.2022H0048)。
文摘The interfacial reaction behavior of Al and Ti_3AlC_(2)at different pouring temperatures and its effect on the microstructure and mechanical properties of the composites were investigated.The results show that the addition of3.0 wt.%Ti_3AlC_(2)refines the average grain size ofα(Al)in the composite by 50.1%compared to Al6061 alloy.Morphological analyses indicate that an in-situ Al_(3Ti)transition layer of-180 nm in thickness is generated around the edge of Ti_3AlC_(2)at 720℃,forming a well-bonded Al-Al_(3Ti)interface.At this processing temperature,the ultimate tensile strength of A16061-3.0 wt.%Ti_3AlC_(2)composite is 199.2 MPa,an improvement of 41.5%over the Al6061 matrix.Mechanism analyses further elucidate that 720℃is favourable for forming the nano-sized transition layer at the Ti_3AlC_(2)edges.And,the thermal mismatch strengthening plays a dominant role in this state,with a strengthening contribution of about 74.8%.
基金Project (ZR2011EL023) supported by the Natural Science Foundation of Shandong Province,ChinaProject (12CX04057A) supported by the Fundamental Research Funds for the Central Universities,China
文摘Al-Ti diffusion couples were made by embedded technology and treated at the temperature between the melting points of Al and Ti. The microstructure evolution and growth mechanism of the Al-Ti DRZ were investigated. The result shows that the DRZ, the mixture of TiAl3 and Al, grows layer by layer along their chemical equilibrium zone. In the course, the growth interface moves toward the aluminum side. TiAl3 is the only new phase which forms earliest in the course of heat-treatment. The growth mechanism of the DRZ changes after the phase transition of titanium. Before the phase transition of titanium, the growth of the DRZ is controlled by the dissolution speed of the titanium to the molten aluminum, while after the phase transition of titanium, the growth is controlled by the chemical reaction speed of Al and Ti atoms, and consequently, its growth rate is greatly increased.
文摘Contact reactive brazing of 6063 Al alloy and 1Cr18Ni9Ti stainless steel was researched by using Cu as interlayer. Effect of brazing time on microstructure of the joints, as well as the dissolution behaviors of Cu interlayer was analyzed. The results show that the product of reaction zone near 1Cr18Ni9Ti is composed of Fe2Al5, FeAl3 intermetallic compound (IMC), and Cu-Al IMC; the near by area is composed of Al-Cu eutectic structure with Al (Cu) solid solution. With increasing the brazing time, the thickness of IMC layer at the interface increases, while the width of Al-Cu eutectic structure with Al(Cu) solution decreases. Calculation shows the dissolution rate of Cu interlayer is very fast. The complete dissolution time is about 0.47 s for Cu interlayer with 10 μm in thickness used in this study.
基金Projects(51074106,51374145)supported by the National Natural Science Foundation of ChinaProject(09JC1408200)supported by the Science and Technology Commission of Shanghai Municipality,China+1 种基金Project(2014M561466)supported by China Postdoctoral Science FoundationProject(14R21411000)supported by Shanghai Postdoctoral Scientific Program,China
文摘AZ31-4.6% Mg2Si (mass fraction) composite was prepared by conventional casting method. Repetitive upsetting (RU) was applied to severely deforming the as-cast composite at 400 ℃ for 1, 3, and 5 passes. Finite element analysis of the material flow indicates that deformation concentrates in the bottom region of the sample after 1 pass, and much more uniform deformation is obtained after 5 passes. During multi-pass RU process, both dendritic and Chinese script type Mg2Si phases are broken up into smaller particles owing to the shear stress forced by the matrix. With the increasing number of RU passes, finer grain size and more homogeneous distribution of Mg2Si particles are obtained along with significant enhancement in both strength and ductility. AZ31-4.6%Mg2Si composite exhibits tensile strength of 284 MPa and elongation of 9.8%after 5 RU passes at 400 ℃ compared with the initial 128 MPa and 5.4%of original AZ31-4.6%Mg2Si composite.
基金The National Natural Science Foundation of China(No.60835001,60875035,60905009,61004032,61004064,11071001)China Postdoctoral Science Foundation(No.201003546)+2 种基金the Ph.D.Programs Foundation of Ministry of Education of China(No.20093401110001)the Major Program of Higher Education of Anhui Province(No.KJ2010ZD02)the Natural Science Research Project of Higher Education of Anhui Province(No.KJ2011A020)
文摘The delay-dependent absolute stability for a class of Lurie systems with interval time-varying delay is studied. By employing an augmented Lyapunov functional and combining a free-weighting matrix approach and the reciprocal convex technique, an improved stability condition is derived in terms of linear matrix inequalities (LMIs). By retaining some useful terms that are usually ignored in the derivative of the Lyapunov function, the proposed sufficient condition depends not only on the lower and upper bounds of both the delay and its derivative, but it also depends on their differences, which has wider application fields than those of present results. Moreover, a new type of equality expression is developed to handle the sector bounds of the nonlinear function, which achieves fewer LMIs in the derived condition, compared with those based on the convex representation. Therefore, the proposed method is less conservative than the existing ones. Simulation examples are given to demonstrate the validity of the approach.
基金Project (JPPT-115-4-1682) supported by the National Defense Science and Technology Industry Committee of China
文摘Equilibrium freezing curve of Mg-2%Ga (mass fraction) alloy was calculated by CALPHALD method. Microstructures of the melted Mg-2%Ga alloys solidified by iron and copper moulds, respectively, were investigated using OM and SEM. Electrochemical properties of the Mg-2%Ga alloys with different freezing rates were measured by galvanostatic, potentiodynamic and electrochemical impedance spectroscopy tests. The results show that solidification by copper mould leads to intergranular MgsGa2 compounds with small size and large number density. Less adsorbent of Mg^+ and oxide corrosion products occur on the surface of the Mg-2%Ga alloys solidified by copper mould, producing lower corrosion current density of 1.8×10^-5 mA/cm^2. In the galvanostatic tests with 100 mA/cm^2 current density, more negative stable potential of-1.604 V exists in the Mg-2%Ga alloys solidified by iron mould due to the lower freezing rate, which leads to smaller inductive and capacity time constants as well as shorter activity time and better electrochemical activity.
基金National Natural Science Foundation of China(30100160,30271179)
文摘Objective: To construct recombinant retroviral vector containing HIV-1 Tatgene and evaluate the junction of the expressed Tat in target cells. Methods: HIV-1 Tat_(101) genewas recovered from pEV plasmid by Hind Ⅲ digestion and cloned into expression plasmid LZESpBMN-Z toconstruct recombinant retroviral expression plasmid named LZRS-Tat_(101). Using the method ofcalcium phosphate, the construct of LZRS-Tat_(101) was then transfected into packaging cell linesPhoenix (ΦNX) which contained env and gal genes encoding structural proteins and pol gene codingfor 3 enzymes ( reverse transcriptase, protease and integrate) essential for retroviral integrationand replication . The stable transfected cell lines was obtained using puromycin to screen for morethan 3 days. Then, immunohistochemical (IHC ) staining was carried out to detect the expressionlevel of Tat_(101) protein in both transiently and stably trancfected ΦNX, respectively. Thesupematants containing recombinant virus collected from transient and stable transfected cells wereemployed to infect 293 cells, respectively, and the expressed Tat in 293 cells was tested by Westernblot. Meantime, the supematants of infected 293 cells was further added to HL3T1 cells which wereHela cell lines containing an HIV-1-LTR/CAT reporter construct to establish a co-culture system.After co-culture for 72 hours, the protein was extracted from HL3T1 cells and used for CAT activityassay. Results: After LZRS- Tat_(101) was transfected into ΦNX, the amount of expressed Tat intransient transfection cells was significantly higher than that in stable transfection cells; Tatcould be detected not only in 293 cells but also in the supematants from 293 cells culture, and Tatin the supematants could activate HIV-1 LTR promoter in HL3T1, resulting in high 'expression of CATlocated at the downstream of LTR. Conclusion: The construct of recombinant retrovirus LZRS-Tat_(101) could express Tat protein in target cells and the expressed Tat was functionally activeand can really exhibit the ability to activate transcription.
文摘A multidisciplinary approach-anatomy, histochemistry and phytochemistry-was used to investigate the leaf structure, the content and the storage location of barbaloin in the leaves of Aloe vera L. var. chinensis (Haw.) Berg. Xeromorphic characteristics including secondary thickened epidermal cell walls, thicker cuticle, ambiguous differentiation of spongy and palisade tissues in the chlorenchyma, and well-developed aquiferous tissue could be seen in the leaves. Several large parenchymatous cells were observed at the phloem pole of the first ring of vascular bundles. The secondary ring of vascular bundles in the leaf base and the stomata, which are surrounded by five cells, have some classification significance in this species. The density of vascular bundles, the content of barbaloin and the intensity of histochemical reaction differed among leaf numbers Ll (annual leaf), L2 (biennial leaf), L3 (triennial leaf) and L4 (quadrennial leaf), and in different parts of the leaf. These three factors were highest in the youngest leaf, Ll, and top parts of all the leaves and lowest in the basal parts and the oldest leaf, L4. The density of vascular bundles had a positive correlation to the content of barbaloin. The histochemical results revealed that the small sheath cells that surrounded the bundles might be the location of barbaloin synthesis and the large parenchymatous cells beneath the sheath might be the storage places of this metabolite.
基金Supported by Project of Common Safety Assessment Technology for Genetically Modified Organisms of the Ministry of Agriculture of PRC (2011ZX08011-006)~~
文摘[Objective] This study aimed to explore an effective method for rapid salt- extraction of high-quality genomic DNA from dried seeds of plants. [Method] Seeds of seven varieties of crops were ground into powder. A hundred milligrams of seed powder was added to extracting solution for high salt-extraction of genomic DNA. The yield and quality of extracted DNA were determined by using ultramicro UV/Vis spectrophotometer detection method, PCR and restriction enzyme digestion. [Result] About 619.67-1 811.21 ng of genomic DNA was extracted from per 100 mg of dried seed powder of seven varieties of conventional crops. A260/A280 ratios of the obtained DNA solution all ranged from 1.87 to 2.07, the purity and quality of PCR were suitable for PCR and restriction enzyme digestion. Clear target bands of specific endogenous gene fragments of seven varieties of crops were amplified by PCR, and the obtained DNA could be fully digested with EcoRV and Hindlll.[Conclusion] This method could be used for rapid extraction of high-quality genomic DNA from dried seeds.
文摘Chloroplast genetic engineering, with several advantages over nuclear genetic engineering, is now regarded as an attractive new technology in basic and applied research, including deepening our understanding of plastid genome, engineering plant metabolic system, generating transplastomic plants with higher resistance to insect, disease, drought and herbicide and bioproducing of antibodies and vaccines. In this review, the principle and operating system for chloroplast genetic engineering and its application in higher plants have been discussed.
基金Project(50971066) supported by the National Natural Science Foundation of ChinaProject(20070299004) supported by Research Fund for the Doctoral Program of Higher Education of China+1 种基金Project(2008-46) supported by Jiangsu Provincial '333' Project of training the High-level Talents Foundation,ChinaProject(BE2009127) supported by Jiangsu Provincial Science Supporting Item,China
文摘(ZrB2+Al2O3+Al3Zr)/A356 composites were synthesized by melt direct reaction from A356-(K2ZrF6+KBF4+Na2B4O7) system.The phase compositions and the microstructures of the as-prepared composites were investigated by XRD,SEM and TEM.The results show that the reinforcements are composed of ZrB2 and Al2O3 ceramic phase particles and Al3Zr intermetallic particles.The ZrB2 particulates are easy to join together to form some particle clusters and distribute along the α(Al) grain boundary.The morphologies of the ZrB2 particulates are in hexagon-shape with the size of about 50 nm.The TEM investigation results of Al3Zr indicate that Al3Zr grows in the form of facet with the length-diameter ratio of about 20.The morphologies of Al2O3 particles are in rectangular-shape and ellipsoidal-shape,with the size of about 0.1 μm.In addition,the interfaces of the matrix and particles are net and no interfacial outgrowth is observed.