Gram-negative bacteria Helicobacter pylori(H. pylori) colonize gastric mucosa in humans and increase the risk of serious diseases such as gastric and duodenal ulcers, stomach cancers and mucosa associated lymphoid tis...Gram-negative bacteria Helicobacter pylori(H. pylori) colonize gastric mucosa in humans and increase the risk of serious diseases such as gastric and duodenal ulcers, stomach cancers and mucosa associated lymphoid tissue lymphoma. The role of H. pylori infection in the pathogenesis of several extragastric diseases has been suggested including immune thrombocytopenic purpura, iron deficiency anemia, vitamin D deficiency, cardiovascular diseases,diabetes mellitus and dermatological disorders. Also neurological diseases and even lung cancer have attracted researchers concern. The relation between H. pylori infection and a growth retardation in children has also been suggested. Many mechanisms of molecular mimicry between H. pylori and the host have been proposed as a pathogen strategy to manipulate the immune system of the host in order to remain unrecognized and avoid eradication. A lot of effort has been put into the demonstration of homologous sequences between H. pylori and host compounds. However, knowledge about how often autoantibodies or autoreactive T lymphocytes induced during H. pylori infections cause pathological disorders is insufficient. This review provides data on H. pylori antigenic mimicry and possible deleterious effects due to the induction of immune response to the components common to these bacteria and the host.展开更多
Objective To evaluate the effects of environmental factors and microRNAs （miRNAs） （miR-126, miR-143, and miR-145） on the risk of coronary heart disease （CHD）. Methods A frequency-matched case-control study （450...Objective To evaluate the effects of environmental factors and microRNAs （miRNAs） （miR-126, miR-143, and miR-145） on the risk of coronary heart disease （CHD）. Methods A frequency-matched case-control study （450 patients, 450 controls） was conducted from April 2014 to December 2016 in Fuzhou City, China. Environmental factors were investigated using a self-administered questionnaire, and the expression levels of miR-126, rniR-143, and miR-145 were determined by quantitative real-time Polymerase Chain Reaction （PCR） in pe- ripheral blood mononuclear cells. Unconditional logistic regression models were used for statistical evaluation. Results Alcohol consumption, high-salt diets, high-intensity work, and lack of physical activity were significantly associated with increased CHD risk, whereas light diet was significantly associated with decreased risk. MiR-126, miR-143, and miR-145 were highly expressed in the CHD group compared with the control group. After adjustment for other environmental factors, unconditional logistic regression results revealed that miR-126, miR-143, and depression were the independent risk factors of CHD, and light diet was the independent protective factor of CHD. Conclusions Our data suggest that a family history of CHD, anxiety, and alcohol consumption was significantly associated with increased CHD risk, whereas light diet was significantly associated with decreased risk. Furthermore, miR-126 and miR-143 in combination with several risk factors, could play a joint role in the development of CHD. Therefore, it is necessary to manage patients with CHD in all directions and multiple level.展开更多
AIM: To investigate whether the side population (SP) cells possess cancer stem cell-like characteristics in vitro and the role of SP cells in tumorigenic process in gastric cancer. METHODS: We analyzed the presence of...AIM: To investigate whether the side population (SP) cells possess cancer stem cell-like characteristics in vitro and the role of SP cells in tumorigenic process in gastric cancer. METHODS: We analyzed the presence of SP cells indifferent human gastric carcinoma cell lines, and then isolated and identified the SP cells from the KATO Ⅲ human gastric cancer cell line by flow cytometry. The clonogenic ability and self-renewal were evaluated by clone and sphere formation assays. The related genes were determined by reverse transcription polymerase chain reaction. To compare tumorigenic ability, SP and non-side population (NSP) cells from the KATO Ⅲ human gastric cancer cell line were subcutaneously injected into nude mice. RESULTS: SP cells from the total population accounted for 0.57% in KATO Ⅲ, 1.04% in Hs-746T, and 0.02% in AGS (CRL-1739). SP cells could grow clonally and have self-renewal capability in conditioned media. The expression of ABCG2, MDRI, Bmi-1 and Oct-4 was different between SP and NSP cells. However, there was no apparent difference between SP and NSP cells when they were injected into nude mice. CONCLUSION: SP cells have some cancer stem celllike characteristics in vitro and can be used for studying the tumorigenic process in gastric cancer.展开更多
For a long time, there have been extensive and deep studies and researches about how to upgrade the level of language education in universities. Now people have realized that the key factor that influences the languag...For a long time, there have been extensive and deep studies and researches about how to upgrade the level of language education in universities. Now people have realized that the key factor that influences the language education most is teachers＇ self-development and the promotion of teachers＇ personalities. A new teaching concept -- reflective teaching has become very popular in the educational circles. It emphasizes that students should become positive and active learners while teachers should bear more dynamic and capacities for self-development.展开更多
Animal intestine is a favorable habitat to microbes. It facilitates the evolution of dense and diversified microbial communities that are highly active and persistent throughout life span. Here, we stimulate this uniq...Animal intestine is a favorable habitat to microbes. It facilitates the evolution of dense and diversified microbial communities that are highly active and persistent throughout life span. Here, we stimulate this unique biosystem to develop high-efficient continuous bio-manufacturing processes. The pig small intestine was explored as a novel bioreactor with industrial Saccharornyces cerevisiae for biofuel production. Results showed that the small intestine was a beneficial material for cell adherence. The cells on the intestine exhibited the abilities of self- immobilization, self-duplication and self-repairing. Therefore the intestine-based S. cerevisiae could be continu- ously used for a long time at high metabolic activities. Both the fermentation speed and ethanol yield were im- proved. This study provides valuable insights into the functions of intestine-based biosystem and should inspire the development of bionic industrial processes. Future dissection of the interface mechanism and design of more bionic materials will make bioprocesses more economically favorable and environmentally sustainable.展开更多
Corrective feedback is crucial for pronunciation teaching.However,in current pronunciation teaching practice,the corrective feedback provided usually fails to locate pronunciation problems and inform learners of the d...Corrective feedback is crucial for pronunciation teaching.However,in current pronunciation teaching practice,the corrective feedback provided usually fails to locate pronunciation problems and inform learners of the differences between their mispronunciations and the correct form.Based on the motor theory,this study attempted to explore a new way of corrective feedback for pronunciation teaching.Specifically,the learners’ speech output was modified and then was played back to them as an input model for learning.In this way,the learners can imitate the pronunciation model of their own voices,achieving self-imitation.This study included two experiments.The first explored the viability of obtaining one’s self-perceived voice through delayed feedback paradigm.The second experiment examined the effectiveness of self-imitation for English intonation learning.Results showed that imitating the pronunciation model of one’s own voice can reduce the learners’ phonological memory load,assist critical listening and facilitate accurate phonetic realizations of the target intonation.展开更多
基金Supported by the National Science Center grants,No.UMO-2013/09/N/NZ6/00805 and No.UMO-2015/17/N/NZ6/03490
文摘Gram-negative bacteria Helicobacter pylori(H. pylori) colonize gastric mucosa in humans and increase the risk of serious diseases such as gastric and duodenal ulcers, stomach cancers and mucosa associated lymphoid tissue lymphoma. The role of H. pylori infection in the pathogenesis of several extragastric diseases has been suggested including immune thrombocytopenic purpura, iron deficiency anemia, vitamin D deficiency, cardiovascular diseases,diabetes mellitus and dermatological disorders. Also neurological diseases and even lung cancer have attracted researchers concern. The relation between H. pylori infection and a growth retardation in children has also been suggested. Many mechanisms of molecular mimicry between H. pylori and the host have been proposed as a pathogen strategy to manipulate the immune system of the host in order to remain unrecognized and avoid eradication. A lot of effort has been put into the demonstration of homologous sequences between H. pylori and host compounds. However, knowledge about how often autoantibodies or autoreactive T lymphocytes induced during H. pylori infections cause pathological disorders is insufficient. This review provides data on H. pylori antigenic mimicry and possible deleterious effects due to the induction of immune response to the components common to these bacteria and the host.
文摘Objective To evaluate the effects of environmental factors and microRNAs （miRNAs） （miR-126, miR-143, and miR-145） on the risk of coronary heart disease （CHD）. Methods A frequency-matched case-control study （450 patients, 450 controls） was conducted from April 2014 to December 2016 in Fuzhou City, China. Environmental factors were investigated using a self-administered questionnaire, and the expression levels of miR-126, rniR-143, and miR-145 were determined by quantitative real-time Polymerase Chain Reaction （PCR） in pe- ripheral blood mononuclear cells. Unconditional logistic regression models were used for statistical evaluation. Results Alcohol consumption, high-salt diets, high-intensity work, and lack of physical activity were significantly associated with increased CHD risk, whereas light diet was significantly associated with decreased risk. MiR-126, miR-143, and miR-145 were highly expressed in the CHD group compared with the control group. After adjustment for other environmental factors, unconditional logistic regression results revealed that miR-126, miR-143, and depression were the independent risk factors of CHD, and light diet was the independent protective factor of CHD. Conclusions Our data suggest that a family history of CHD, anxiety, and alcohol consumption was significantly associated with increased CHD risk, whereas light diet was significantly associated with decreased risk. Furthermore, miR-126 and miR-143 in combination with several risk factors, could play a joint role in the development of CHD. Therefore, it is necessary to manage patients with CHD in all directions and multiple level.
文摘AIM: To investigate whether the side population (SP) cells possess cancer stem cell-like characteristics in vitro and the role of SP cells in tumorigenic process in gastric cancer. METHODS: We analyzed the presence of SP cells indifferent human gastric carcinoma cell lines, and then isolated and identified the SP cells from the KATO Ⅲ human gastric cancer cell line by flow cytometry. The clonogenic ability and self-renewal were evaluated by clone and sphere formation assays. The related genes were determined by reverse transcription polymerase chain reaction. To compare tumorigenic ability, SP and non-side population (NSP) cells from the KATO Ⅲ human gastric cancer cell line were subcutaneously injected into nude mice. RESULTS: SP cells from the total population accounted for 0.57% in KATO Ⅲ, 1.04% in Hs-746T, and 0.02% in AGS (CRL-1739). SP cells could grow clonally and have self-renewal capability in conditioned media. The expression of ABCG2, MDRI, Bmi-1 and Oct-4 was different between SP and NSP cells. However, there was no apparent difference between SP and NSP cells when they were injected into nude mice. CONCLUSION: SP cells have some cancer stem celllike characteristics in vitro and can be used for studying the tumorigenic process in gastric cancer.
文摘For a long time, there have been extensive and deep studies and researches about how to upgrade the level of language education in universities. Now people have realized that the key factor that influences the language education most is teachers＇ self-development and the promotion of teachers＇ personalities. A new teaching concept -- reflective teaching has become very popular in the educational circles. It emphasizes that students should become positive and active learners while teachers should bear more dynamic and capacities for self-development.
文摘Animal intestine is a favorable habitat to microbes. It facilitates the evolution of dense and diversified microbial communities that are highly active and persistent throughout life span. Here, we stimulate this unique biosystem to develop high-efficient continuous bio-manufacturing processes. The pig small intestine was explored as a novel bioreactor with industrial Saccharornyces cerevisiae for biofuel production. Results showed that the small intestine was a beneficial material for cell adherence. The cells on the intestine exhibited the abilities of self- immobilization, self-duplication and self-repairing. Therefore the intestine-based S. cerevisiae could be continu- ously used for a long time at high metabolic activities. Both the fermentation speed and ethanol yield were im- proved. This study provides valuable insights into the functions of intestine-based biosystem and should inspire the development of bionic industrial processes. Future dissection of the interface mechanism and design of more bionic materials will make bioprocesses more economically favorable and environmentally sustainable.
文摘Corrective feedback is crucial for pronunciation teaching.However,in current pronunciation teaching practice,the corrective feedback provided usually fails to locate pronunciation problems and inform learners of the differences between their mispronunciations and the correct form.Based on the motor theory,this study attempted to explore a new way of corrective feedback for pronunciation teaching.Specifically,the learners’ speech output was modified and then was played back to them as an input model for learning.In this way,the learners can imitate the pronunciation model of their own voices,achieving self-imitation.This study included two experiments.The first explored the viability of obtaining one’s self-perceived voice through delayed feedback paradigm.The second experiment examined the effectiveness of self-imitation for English intonation learning.Results showed that imitating the pronunciation model of one’s own voice can reduce the learners’ phonological memory load,assist critical listening and facilitate accurate phonetic realizations of the target intonation.
