目的以灌注成像为参照,探讨MRI液体衰减反转恢复序列血管高信号征(FVH)与弥散加权成像(DWI)不匹配征象在急性前循环大血管闭塞性缺血性卒中血管内取栓术(EVT)前评估中的作用。方法回顾性分析2020年5月至2021年10月就诊于天津市环湖医院...目的以灌注成像为参照,探讨MRI液体衰减反转恢复序列血管高信号征(FVH)与弥散加权成像(DWI)不匹配征象在急性前循环大血管闭塞性缺血性卒中血管内取栓术(EVT)前评估中的作用。方法回顾性分析2020年5月至2021年10月就诊于天津市环湖医院神经外科、发病时间为6~24 h的急性前循环大血管闭塞性缺血性卒中患者的临床资料,共91例。患者均同时接受MRI平扫和灌注成像(CT灌注成像或MRI灌注加权成像)检查,分别应用两种影像筛选模式判断EVT指征。其中MRI平扫判断有EVT指征的标准为存在FVH-DWI不匹配;灌注成像判断EVT指征的标准为梗死核心体积与缺血半暗带不匹配。评价两种影像筛选模式判断EVT指征的一致性,以及经两种方法筛选后行EVT患者术后90 d预后良好[改良Rankin量表评分(mRS)≤2分]率的差异。结果91例患者中,应用FVH-DWI不匹配筛选73例(80.2%)存在EVT指征,其中49例行EVT,术后90 d 28例(57.1%)预后良好。应用灌注成像不匹配筛选75例(82.4%)存在EVT指征,其中55例行EVT,术后90 d 28例(50.9%)预后良好。70例患者同时存在FVH-DWI不匹配和灌注不匹配,13例FVH-DWI和灌注不匹配均不存在,5例存在灌注不匹配而无FVH-DWI不匹配,3例存在FVH-DWI不匹配而无灌注不匹配。根据FVH-DWI不匹配确定EVT指征与根据灌注不匹配确定EVT指征的差异无统计学意义(P=0.724),一致性较好(κ=0.71,95%CI:0.52~0.90);术后90 d预后良好率差异无统计学意义(P=0.660)。同时存在FVH-DWI不匹配和灌注不匹配者术后预后良好占比最高,FVH-DWI不匹配和灌注不匹配均不存在者术后预后良好占比最低。结论MRI平扫FVH-DWI不匹配征象是急性前循环大血管闭塞缺血性卒中患者术前判断EVT指征简单且较为可靠的方法。展开更多
NAD(P)H oxidases were detected in suspension cultured cells of ginseng (Panax ginseng C. A. Meyer). The activities of these enzymes were induced by an elicitor (Cle) extracted from cell walls of Col-letotrichum lagera...NAD(P)H oxidases were detected in suspension cultured cells of ginseng (Panax ginseng C. A. Meyer). The activities of these enzymes were induced by an elicitor (Cle) extracted from cell walls of Col-letotrichum lagerarium. In addition, Cle induced an oxidative burst and enhanced the synthesis of saponin, activity of phenylalanine ammonialyase (PAL) , accumulation of chalcone synthase (CHS) and the transcription of a hydroxyproline-rich glycoprotein gene ( hrgp ) . Pre-treatments with DPI and quinacrine (two inhibitors of mammalian neutrophil plasma membrane NADPH oxidase) for 30 min prior to Cle addition blocked the NAD(P)H oxidase activity induced by Cle. These inhibitors also inhibited the release of H2C2, the synthesis of saponin, PAL activity and CHS accumulation. Our data revealed homology between plasma membrane NAD(P)H oxidases of mammalian neutrophil cells and ginseng suspension cells. They also indicated that deactivated NAD(P)H oxidases catalysed the release of H2O2 and that H2O2 was functioning as a second messenger stimulating PAL activity, saponin synthesis and hrgp transcription. Elevations of Ca2 + and protein phos-phorylation/dephosphorylation were required for this defense process. We propose that NAD(P)H oxidases mediate the processes of Cle-induced defense responses in ginseng suspensions, and postulate the existence of a signalling cascade including extracellular Cle stimulation, activation of plasma membrane NAD(P)H oxidases, release of H2O2, and the intracellular responses of metabolism and gene transcription in ginseng suspension cells.展开更多
The relationship between Ca 2+ and ethylene response was investigated through analyzing the effect of Ca 2+ on the response to ethylene in etiolated tomato (Lycopersicon esculentum Mill cv. Lichun) seedling grow...The relationship between Ca 2+ and ethylene response was investigated through analyzing the effect of Ca 2+ on the response to ethylene in etiolated tomato (Lycopersicon esculentum Mill cv. Lichun) seedling grown in darkness. When the etiolated tomato seedlings were treated with different concentrations of Ca 2+, the 'triple response' phenotype, ethylene production, the expression of ethylene receptor gene NEVER-RIPE (NR) and the content of cytosolic CaM were determined. With the concentration of Ca 2+ in the culture medium increasing from 0 mmol/L to 3.8 mmol/L, the 'triple response' phenotype of etiolated tomato seedling was correspondingly strengthened; meanwhile the ethylene production, the amount of NR gene expression and the concentration of CaM increased respectively. However, when the concentration of Ca 2+ was increased from 3.8 mmol/L to 10 mmol/L, the phenotype of 'triple response', ethylene production, NR gene expression, and the CaM content didn't increase further, but decreased consequently. The results indicated that the effect of Ca 2+ on the ethylene triple response in etiolated tomato seedling was relevant to ethylene biosynthesis and ethylene receptor gene expression which were influenced by applied Ca 2+, and these effects might be mediated through the change of CaM concentration in plant cell.展开更多
AIM： To explore the effect of Astraga/us mongholicus polysaccharide （APS） on gene expression and mitogenactivated protein kinase （MAPK） transcriptional activity in intestinal epithelial cells （IEC）. METHODS： I...AIM： To explore the effect of Astraga/us mongholicus polysaccharide （APS） on gene expression and mitogenactivated protein kinase （MAPK） transcriptional activity in intestinal epithelial cells （IEC）. METHODS： IEC were divided into control group, lipopolysaccharide （LPS） group, LPS＋ 50 μg/mL APS group, LPS＋ 100 μg/mL APS group, LPS＋ 200 μg/mL APS group, and LPS＋ 500 μg/mL APS group. Levels of mRNAs in LPS-induced inflammatory factors, tumor necrosis factor （TNF）-α and interleukin （IL）-8, were measured by reverse transcription-polymerase chain reaction. MAPK protein level was measured by Western blotting. RESULTS： The levels of TNF-α and IL-8 mRNAs were significantly higher in IEC with LPS-induced damage than in control cells. APS significantly abrogated the LPS-induced expression of the TNF-α and IL-8 genes. APS did not block the activation of extracellular signal- regulated kinase or c Jun amino-terminal kinase, but inhibited the activation of p38, suggesting that APS inhibits LPS-induced production of TNF-α and IL-8 mRNAs, possibly by suppressing the p38 signaling pathway.CONCLUSION： APS-modulated bacterial productmediated p38 signaling represents an attractive strategy for prevention and treatment of intestinal inflammation.展开更多
AIM： To analyze the relevance of the microRNA miR-196a for colorectal oncogenesis.METHODS： The impact of miR-196a on the restriction targets HoxA7, HoxBS, HoxC8 and HoxD8 was analyzed by reverse transcription polyme...AIM： To analyze the relevance of the microRNA miR-196a for colorectal oncogenesis.METHODS： The impact of miR-196a on the restriction targets HoxA7, HoxBS, HoxC8 and HoxD8 was analyzed by reverse transcription polymerase chain reaction（RT-PCR） after transient transfection of SW480 cancer cells. The miR-196a transcription profile in colorectalcancer samples, mucosa samples and diverse cancercell lines was quantified by RT-PCR. Transiently miR-196a-transfected colorectal cancer cells were used for diverse functional assays in vitro and for a xenograft lung metastasis model in vivo.RESULTS： HoxA7, HoxB8, HoxC8 and HoxD8 wererestricted by miR-196a in a dose-dependent andgene-specific manner. High levels of miR-196aactivated the AKT signaling pathway as indicated byincreased phosphorylation of AKT. In addition, highlevels of miR-196a promoted cancer cell detachment,migration, invasion and chemosensitivity towardsplatin derivatives but did not impact on proliferationor apoptosis. Furthermore, miR-196a increased thedevelopment of lung metastases in mice after tail veininjection.展开更多
文摘目的以灌注成像为参照,探讨MRI液体衰减反转恢复序列血管高信号征(FVH)与弥散加权成像(DWI)不匹配征象在急性前循环大血管闭塞性缺血性卒中血管内取栓术(EVT)前评估中的作用。方法回顾性分析2020年5月至2021年10月就诊于天津市环湖医院神经外科、发病时间为6~24 h的急性前循环大血管闭塞性缺血性卒中患者的临床资料,共91例。患者均同时接受MRI平扫和灌注成像(CT灌注成像或MRI灌注加权成像)检查,分别应用两种影像筛选模式判断EVT指征。其中MRI平扫判断有EVT指征的标准为存在FVH-DWI不匹配;灌注成像判断EVT指征的标准为梗死核心体积与缺血半暗带不匹配。评价两种影像筛选模式判断EVT指征的一致性,以及经两种方法筛选后行EVT患者术后90 d预后良好[改良Rankin量表评分(mRS)≤2分]率的差异。结果91例患者中,应用FVH-DWI不匹配筛选73例(80.2%)存在EVT指征,其中49例行EVT,术后90 d 28例(57.1%)预后良好。应用灌注成像不匹配筛选75例(82.4%)存在EVT指征,其中55例行EVT,术后90 d 28例(50.9%)预后良好。70例患者同时存在FVH-DWI不匹配和灌注不匹配,13例FVH-DWI和灌注不匹配均不存在,5例存在灌注不匹配而无FVH-DWI不匹配,3例存在FVH-DWI不匹配而无灌注不匹配。根据FVH-DWI不匹配确定EVT指征与根据灌注不匹配确定EVT指征的差异无统计学意义(P=0.724),一致性较好(κ=0.71,95%CI:0.52~0.90);术后90 d预后良好率差异无统计学意义(P=0.660)。同时存在FVH-DWI不匹配和灌注不匹配者术后预后良好占比最高,FVH-DWI不匹配和灌注不匹配均不存在者术后预后良好占比最低。结论MRI平扫FVH-DWI不匹配征象是急性前循环大血管闭塞缺血性卒中患者术前判断EVT指征简单且较为可靠的方法。
文摘目的探讨液体衰减反转恢复序列血管高信号(fluid-attenuated inversion recovery vascular hyperintensity,FVH)在未接受再灌注治疗的轻度症状大脑中动脉闭塞的卒中患者预后中的价值。材料与方法前瞻性纳入2017年1月至2019年3月在本院就诊的具有轻度症状的急性卒中患者[入院美国国立卫生研究院卒中量表(National Institute of Health stroke scale;NIHSS)评分≤5分]。所有患者均于治疗前接受磁共振成像检查,且经磁共振血管造影(MR angiography,MRA)检查为大脑中动脉闭塞。根据FVH显著存在与否,将FVH分为两组:FVH(-)和FVH(+)组。收集所有患者的弥散张量成像(diffusionweighted imaging,DWI)梗死体积、3个月功能功能预后(mRS评分)及一般临床资料等。统计学分析FVH在未接受再灌注治疗的轻度症状大脑中动脉闭塞的卒中患者预后中的价值。结果在未接受再灌注治疗的患者中(48例),FVH(-)组FVH-DWI不匹配率(23.81%)、不良功能预后比例(14.29%)低于FVH(+)组(66.67%;51.85%),差异有统计学意义(t=8.694,P=0.004;t=7.288,P=0.014)。与预后良好组相比,不良功能预后组具有较低的FVH-DWI不匹配率(23.81%与61.29%)、较大的DWI梗死体积(6.60±1.11与4.43±2.20)及较高的FVH(+)率(82.35%与41.94%),差异有统计学意义(t=6.273,P=0.017;t=4.447,P=0.000;t=7.288,P=0.014)。逻辑回归分析结果显示DWI梗死体积[OR(95%CI)]:0.327[(0.154~0.698),P=0.004]、FVH[OR(95%CI)]:6.462[(1.536~27.179),P=0.011]及FVH-DWI不匹配[OR(95%CI)]:0.099[(0.025~0.389),P=0.001]为预测未接受再灌注治疗患者卒中预后的独立预测因子。结论对于未接受再灌注治疗的轻度症状大脑中动脉闭塞的卒中患者,FVH显著比FVH不显著的患者更可能出现不良的功能预后。
文摘NAD(P)H oxidases were detected in suspension cultured cells of ginseng (Panax ginseng C. A. Meyer). The activities of these enzymes were induced by an elicitor (Cle) extracted from cell walls of Col-letotrichum lagerarium. In addition, Cle induced an oxidative burst and enhanced the synthesis of saponin, activity of phenylalanine ammonialyase (PAL) , accumulation of chalcone synthase (CHS) and the transcription of a hydroxyproline-rich glycoprotein gene ( hrgp ) . Pre-treatments with DPI and quinacrine (two inhibitors of mammalian neutrophil plasma membrane NADPH oxidase) for 30 min prior to Cle addition blocked the NAD(P)H oxidase activity induced by Cle. These inhibitors also inhibited the release of H2C2, the synthesis of saponin, PAL activity and CHS accumulation. Our data revealed homology between plasma membrane NAD(P)H oxidases of mammalian neutrophil cells and ginseng suspension cells. They also indicated that deactivated NAD(P)H oxidases catalysed the release of H2O2 and that H2O2 was functioning as a second messenger stimulating PAL activity, saponin synthesis and hrgp transcription. Elevations of Ca2 + and protein phos-phorylation/dephosphorylation were required for this defense process. We propose that NAD(P)H oxidases mediate the processes of Cle-induced defense responses in ginseng suspensions, and postulate the existence of a signalling cascade including extracellular Cle stimulation, activation of plasma membrane NAD(P)H oxidases, release of H2O2, and the intracellular responses of metabolism and gene transcription in ginseng suspension cells.
文摘The relationship between Ca 2+ and ethylene response was investigated through analyzing the effect of Ca 2+ on the response to ethylene in etiolated tomato (Lycopersicon esculentum Mill cv. Lichun) seedling grown in darkness. When the etiolated tomato seedlings were treated with different concentrations of Ca 2+, the 'triple response' phenotype, ethylene production, the expression of ethylene receptor gene NEVER-RIPE (NR) and the content of cytosolic CaM were determined. With the concentration of Ca 2+ in the culture medium increasing from 0 mmol/L to 3.8 mmol/L, the 'triple response' phenotype of etiolated tomato seedling was correspondingly strengthened; meanwhile the ethylene production, the amount of NR gene expression and the concentration of CaM increased respectively. However, when the concentration of Ca 2+ was increased from 3.8 mmol/L to 10 mmol/L, the phenotype of 'triple response', ethylene production, NR gene expression, and the CaM content didn't increase further, but decreased consequently. The results indicated that the effect of Ca 2+ on the ethylene triple response in etiolated tomato seedling was relevant to ethylene biosynthesis and ethylene receptor gene expression which were influenced by applied Ca 2+, and these effects might be mediated through the change of CaM concentration in plant cell.
文摘AIM： To explore the effect of Astraga/us mongholicus polysaccharide （APS） on gene expression and mitogenactivated protein kinase （MAPK） transcriptional activity in intestinal epithelial cells （IEC）. METHODS： IEC were divided into control group, lipopolysaccharide （LPS） group, LPS＋ 50 μg/mL APS group, LPS＋ 100 μg/mL APS group, LPS＋ 200 μg/mL APS group, and LPS＋ 500 μg/mL APS group. Levels of mRNAs in LPS-induced inflammatory factors, tumor necrosis factor （TNF）-α and interleukin （IL）-8, were measured by reverse transcription-polymerase chain reaction. MAPK protein level was measured by Western blotting. RESULTS： The levels of TNF-α and IL-8 mRNAs were significantly higher in IEC with LPS-induced damage than in control cells. APS significantly abrogated the LPS-induced expression of the TNF-α and IL-8 genes. APS did not block the activation of extracellular signal- regulated kinase or c Jun amino-terminal kinase, but inhibited the activation of p38, suggesting that APS inhibits LPS-induced production of TNF-α and IL-8 mRNAs, possibly by suppressing the p38 signaling pathway.CONCLUSION： APS-modulated bacterial productmediated p38 signaling represents an attractive strategy for prevention and treatment of intestinal inflammation.
基金Supported by The University of Mainz Project Grant
文摘AIM： To analyze the relevance of the microRNA miR-196a for colorectal oncogenesis.METHODS： The impact of miR-196a on the restriction targets HoxA7, HoxBS, HoxC8 and HoxD8 was analyzed by reverse transcription polymerase chain reaction（RT-PCR） after transient transfection of SW480 cancer cells. The miR-196a transcription profile in colorectalcancer samples, mucosa samples and diverse cancercell lines was quantified by RT-PCR. Transiently miR-196a-transfected colorectal cancer cells were used for diverse functional assays in vitro and for a xenograft lung metastasis model in vivo.RESULTS： HoxA7, HoxB8, HoxC8 and HoxD8 wererestricted by miR-196a in a dose-dependent andgene-specific manner. High levels of miR-196aactivated the AKT signaling pathway as indicated byincreased phosphorylation of AKT. In addition, highlevels of miR-196a promoted cancer cell detachment,migration, invasion and chemosensitivity towardsplatin derivatives but did not impact on proliferationor apoptosis. Furthermore, miR-196a increased thedevelopment of lung metastases in mice after tail veininjection.
