Based on the synchronous joint gravity and magnetic inversion of single interface by Pilkington and the need of revealing Cenozoic and crystalline basement thickness in the new round of oil-gas exploration, we propose...Based on the synchronous joint gravity and magnetic inversion of single interface by Pilkington and the need of revealing Cenozoic and crystalline basement thickness in the new round of oil-gas exploration, we propose a joint gravity and magnetic inversion methodfor two-layer models by concentrating on the relationship between the change of thicknessI and position of the middle layer and anomaly and discuss the effects of the key parameters. Model tests and application to field data show the validity of this method.展开更多
Pretreatment on vegetable and fruit is a key chain of drying process. The research analyzed mechanisms and applications of different pretreatments such as blanching, freezing, liquid glucose pretreatment, NaCI dipping...Pretreatment on vegetable and fruit is a key chain of drying process. The research analyzed mechanisms and applications of different pretreatments such as blanching, freezing, liquid glucose pretreatment, NaCI dipping, and high-voltage elec- tric field, and proposed the development prospect.展开更多
The development of unconventional resources, such as shale gas and tight sane gas, requires the integration of multi-disciplinary knowledge to resolve many engineering problems in order to achieve economic production ...The development of unconventional resources, such as shale gas and tight sane gas, requires the integration of multi-disciplinary knowledge to resolve many engineering problems in order to achieve economic production levels. The reservoir heterogeneit3 revealed by different data sets, such as 3D seismic and microseismic data, can more full3 reflect the reservoir properties and is helpful to optimize the drilling and completioT programs. First, we predict the local stress direction and open or close status of the natura fractures in tight sand reservoirs based on seismic curvature, an attribute that reveals reservoi heterogeneity and geomechanical properties. Meanwhile, the reservoir fracture network is predicted using an ant-tracking cube and the potential fracture barriers which can affec hydraulic fracture propagation are predicted by integrating the seismic curvature attribute anc ant-tracking cube. Second, we use this information, derived from 3D seismic data, to assis in designing the fracture program and adjusting stimulation parameters. Finally, we interpre the reason why sand plugs will occur during the stimulation process by the integration of 3E seismic interpretation and microseismic imaging results, which further explain the hydraulic fracure propagation controlling factors and open or closed state of natural fractures in tigh sand reservoirs.展开更多
This paper focus on the ratoon rice, which with the features of south Chi-na paddy cultivation technology. First, the development of the technology research on ratoon rice in southwest ecotope of China was briefly rev...This paper focus on the ratoon rice, which with the features of south Chi-na paddy cultivation technology. First, the development of the technology research on ratoon rice in southwest ecotope of China was briefly reviewed. Second, the key technical measures of the ratoon rice production were described in detail. At last, the application effect of the ratoon rice production technology was described accu-rately, which based on the production practice in recent years. The purpose of this article is promoting the research and extension of the ratoon rice technology more widely in world’s main rice-producing areas, especial y in the suitable area where the light and heat resources are rich.展开更多
Aim: To compare the contents of nucleosides from natural Cordyceps sinensis and cultured Cordyceps mycelia, and to study the effect of humidity and heat on the content of nucleosides. Methods: The contents of nucleos...Aim: To compare the contents of nucleosides from natural Cordyceps sinensis and cultured Cordyceps mycelia, and to study the effect of humidity and heat on the content of nucleosides. Methods: The contents of nucleosides were determined by using high performance capillary electrophoresis (HPCE). Beckman P/ACE System 5010 apparatus equipped with a UV detector and a Beckman untreated fused-silica capillary (57 cm 75 mm, 50 cm effective length) was used. Before sample injection, the capillary was rinsed with 1 molL-1 sodium hydroxide solution and running buffer for 5 min, respectively. A voltage of 20 kV was applied for the separation. Pressure injection was 586 kPa for 6 seconds, and the wavelength of detector was 254 nm. The running time was 20 min at 20 oC. The effect of humidity and heat on the contents of nucleosides from natural Cordyceps sinensis and cultured Cordyceps mycelia was observed for 1, 3, 5 and 10 days at temperature 40 oC, and relative humidity 75%. Results: The content of nucleosides from natural Cordyceps sinensis was higher than that from cultured Cordyceps mycelia. But the contents of nucleosides from freshly collected natural Cordyceps sinensis were very low, even below the limit of quantitation. The contents of nucleosides from natural Cordyceps sinensis were significantly increased by humidity and heat, but this phenomenon was not observed in cultured Cordyceps mycelia. Conclusion: There are differences between the nucleosides from natural Cordyceps sinensis and cultured Cordyceps mycelia. The nucleosides in natural Cordyceps sinensis may be derived from the degradation of nucleic acids. This implies that adenosine being used for the quality control of natural Cordyceps sinensis may have to be reconsidered.展开更多
[Objective] The aim was to isolate and identify a taxol-producing endophytic fungus from Taxus media. [Method] 32 strains of endophytic fungi were identified form the inner bark of T. media,and their fermentation prod...[Objective] The aim was to isolate and identify a taxol-producing endophytic fungus from Taxus media. [Method] 32 strains of endophytic fungi were identified form the inner bark of T. media,and their fermentation products were detected by high performance liquid chromatography (HPLC). [Result] Through the screening,a strain of taxol-producing endophytic fungi M57 was obtained,which could produce 45-50 μg/L of taxol,and M57 was defined as Rhizopus sp. through morphological observation and 18S rDNA sequence analysis. [Conclusion] The finding of Rhizopus sp. M57 provided a promising strain for producing taxol with taxol-producing fungi fermentation process.展开更多
The research explored adaptability maize seed coating agent in spring sowing and effects of ultra-fine powder shaped areas in northeast China. The results showed that germination potential and rate both improved aroun...The research explored adaptability maize seed coating agent in spring sowing and effects of ultra-fine powder shaped areas in northeast China. The results showed that germination potential and rate both improved around 5% and the num- ber of root increased by 4% in the treatments with ultra-fine powder shaped maize seed coating agent compared with the control group without seed coating. What's more, the treatments with ultra-fine powder shaped maize seed coating agent took advantages in terms of fresh weight of seeding, stem diameter, and dry weight. It is notable that control effects on underground insects performed the best, with per- centage over 94%, which is more excellent relative to other agents in markets. Therefore, ultra-fine powder shaped maize seed coating agent can be widely applied in spring sowing areas in northeast China, without side effects.展开更多
The paper analyzes the definite place of industrial building in the history of urban development. Due to the development of urban economy and the transition of the traditional industrial structure, many historic citie...The paper analyzes the definite place of industrial building in the history of urban development. Due to the development of urban economy and the transition of the traditional industrial structure, many historic cities and towns both at home and abroad have witnessed a large scale "demolition" and abandonment of industrial buildings in urban renewal during last 30 years. Consequently, it has been leading to the discontinuity of urban cultural and historic context. The paper discusses and expounds the clas...展开更多
Fenlong green ecological agriculture technology (Fenlong technology), a new smash ridging farming method developed by Guangxi Academy of Agdcultural Sciences, has been elected as the recommended cultivation techniqu...Fenlong green ecological agriculture technology (Fenlong technology), a new smash ridging farming method developed by Guangxi Academy of Agdcultural Sciences, has been elected as the recommended cultivation technique by the Ministry of Agriculture of China. It replaces the traditional plowshare with spiral drill, and its tilth depth is twice deeper than that by tractor tilthing. It also extends soil nutrient, moisture, oxygen and microorganism, the so-called "Four pools". Soil nutrient, oxygen, microorganism, light and rainfall use ratio is increased by 10%-100%, creating a platform for natural increase of more than 10% of crop yield. Its application to over 20 kinds of crops in 21 provinces has proved that the yield increases 10-30% with quality enhancing 5% and double water retaining capacity but no more input. When the application area of Fenlong could reach 67 million hm2, the amount of fertilizer can be reduced by 40-50 billion kg, saving 120-150 billion Yuan. In this paper, we put forward the strategy of "4+1" (arable, saline-alkali soil, grasslands, Sponge City + rivers) green development in China, and deepened the Fenlong cultivated tilled layer from 16.5 cm to 35 cm for 67 million hm2 arable land, ridged 13.3 million hm2 of saline-alkali soil for 35 cm, and also 35 cm for 67 million hm2 degraded steppe, which could have the following 3 effects: first, the 147 million hm2 of land with Fenlong cultivation could increase loosing soil to 315.491 billion m3, in* creasing by 159.26% for 120 million hm2 of arable land with the average tilled layer of 16.5 cm, which has loosing soil of only 198.1 billion m3, that is, the space of the land increases 1.6 times. Second, every hectare of plowland could store up to 450 m3/hm2 of natural rainfall, and the unused 60 m3 of saline-alkali soil and grasslands could store water of 102 billion m3, showing an increase of over 88.89% for the current plowland storage of 54 billion m3 at now, that is, double the natural rainfall storage capacity. Third, the two multiple increase of natural resources application can bring trillions of resource activation, environmental cleaning, food security, citizens, health, economic, ecological and social benefits, and makes the Chinese nation move forward in green development. Its application in "big scientific research" and "One Belt And One Road" will contribute Chinese strength to the world.展开更多
In order to develop an anti-FMDV Asial type monoclonal antibody (mAb), BABL/c mice were immunized with recombinant FMDV VP1 protein. Three mAbs, 1B8, 5El and 5E2, were then further optimized. The result indicated th...In order to develop an anti-FMDV Asial type monoclonal antibody (mAb), BABL/c mice were immunized with recombinant FMDV VP1 protein. Three mAbs, 1B8, 5El and 5E2, were then further optimized. The result indicated that prepared anti-FMDV Asial mAbs had no cross-reactivity with Swine vesicular disease (SVD) and FMDV O, A and C type antigen. Their titers in abdomen liquor were 1:5×10^6, 1:2×10^6 and 1:5×10^6, respectively. 1B8 was found to be of IgG1 subtype, 5El and 5E2 belonged to IgG2b subtype. In this study, the prepared mAbs are specific for detecting FMDV type Asia1, and is potentially useful for pen-side diagnosis.展开更多
A model of an irreversible proton exchange membrane (PEM) fuel cell working at steady-state was established, in which overpotenfials, internal currents, and crossover losses were taken into account. The expressions ...A model of an irreversible proton exchange membrane (PEM) fuel cell working at steady-state was established, in which overpotenfials, internal currents, and crossover losses were taken into account. The expressions of some key parameters of the fuel cell were derived from the point of electrochemistry and thermodynamics. Based on the irreversible model of the PEM fuel ceil, the influence of multi-irreversibilities on fuel cell performance were characterized and compared systematically. The general performance characteristic curves were generated. Moreover, when the electrical circuit was dosed with a load in it, the relations between the load resistance and power output density and efficiency were analyzed. The results provide a theoretical basis for both the operation and optimal design of real PEM fuel cells.展开更多
A synthetic technology for the production of methyl-ethyl-ketone (MEK) is introduced, which makes use of butylene catalytic hydration to produce secondary butanol (SBA) and this is followed by dehydrogenation of SBA. ...A synthetic technology for the production of methyl-ethyl-ketone (MEK) is introduced, which makes use of butylene catalytic hydration to produce secondary butanol (SBA) and this is followed by dehydrogenation of SBA. By optimizing the operating conditions of a commercial unit, the running results showed that the recom-mended process was successful and that the catalysts had high activity, good selectivity, and long lifespan, which ensured long-term running in commercial units.展开更多
1,3-Propanediol,traditionally obtained from fossils,has numerous industrial applications,including use in the production of high performance polymers.The microbial production of 1,3-propanediol presents several opport...1,3-Propanediol,traditionally obtained from fossils,has numerous industrial applications,including use in the production of high performance polymers.The microbial production of 1,3-propanediol presents several opportunities,and the final purity grade determines its price and commercial viability.The development of novel separation technology could improve the economic viability of the bioproduction of 1,3-propanediol.Thus,we investigated salting-out extraction as a novel process for 1,3-propanediol recovery from fermentation broth.Initially,a screening for the best salt/solvent combination was conducted and then optimized using the response surface methodology.The solvents studied were methanol,ethanol,isopropanol and acetone,and the salts examined were K_2HPO_4,Na_2CO_3,K_2CO_3,(NH_4)_2SO_4,NaHPO_4,K_3PO_4 and C_6H_5NaO_7.The optimal extraction system consisted of 34 wt%K_3PO_4,28 wt% ethanol,and 38 wt% fermentation broth containing 23.0 g·L^(-1)1,3-propanediol,which gave the highest partition coefficient of 33 and recovery yield of 97%.The results demonstrated that salting-out extraction was a promising method for 1,3-propanediol recovery from fermentation broth.展开更多
基金Supported by the National Natural Science Foundation of China(Grant No.40674063)National Hi-tech Research and Development Program of China(863Program)(Grant No.2006AA09Z311)
文摘Based on the synchronous joint gravity and magnetic inversion of single interface by Pilkington and the need of revealing Cenozoic and crystalline basement thickness in the new round of oil-gas exploration, we propose a joint gravity and magnetic inversion methodfor two-layer models by concentrating on the relationship between the change of thicknessI and position of the middle layer and anomaly and discuss the effects of the key parameters. Model tests and application to field data show the validity of this method.
基金Supported by National Natural Science Foundation of China(31360399)~~
文摘Pretreatment on vegetable and fruit is a key chain of drying process. The research analyzed mechanisms and applications of different pretreatments such as blanching, freezing, liquid glucose pretreatment, NaCI dipping, and high-voltage elec- tric field, and proposed the development prospect.
文摘The development of unconventional resources, such as shale gas and tight sane gas, requires the integration of multi-disciplinary knowledge to resolve many engineering problems in order to achieve economic production levels. The reservoir heterogeneit3 revealed by different data sets, such as 3D seismic and microseismic data, can more full3 reflect the reservoir properties and is helpful to optimize the drilling and completioT programs. First, we predict the local stress direction and open or close status of the natura fractures in tight sand reservoirs based on seismic curvature, an attribute that reveals reservoi heterogeneity and geomechanical properties. Meanwhile, the reservoir fracture network is predicted using an ant-tracking cube and the potential fracture barriers which can affec hydraulic fracture propagation are predicted by integrating the seismic curvature attribute anc ant-tracking cube. Second, we use this information, derived from 3D seismic data, to assis in designing the fracture program and adjusting stimulation parameters. Finally, we interpre the reason why sand plugs will occur during the stimulation process by the integration of 3E seismic interpretation and microseismic imaging results, which further explain the hydraulic fracure propagation controlling factors and open or closed state of natural fractures in tigh sand reservoirs.
基金Supported by Chinese Agriculture Special Scientific Research(200903002)The SpecialFunds for Major Chongqing Science and Technology Projects(cstc2012ggB8005)The Special Funds for Chongqing Agriculture Scientific Research and Extention~~
文摘This paper focus on the ratoon rice, which with the features of south Chi-na paddy cultivation technology. First, the development of the technology research on ratoon rice in southwest ecotope of China was briefly reviewed. Second, the key technical measures of the ratoon rice production were described in detail. At last, the application effect of the ratoon rice production technology was described accu-rately, which based on the production practice in recent years. The purpose of this article is promoting the research and extension of the ratoon rice technology more widely in world’s main rice-producing areas, especial y in the suitable area where the light and heat resources are rich.
文摘Aim: To compare the contents of nucleosides from natural Cordyceps sinensis and cultured Cordyceps mycelia, and to study the effect of humidity and heat on the content of nucleosides. Methods: The contents of nucleosides were determined by using high performance capillary electrophoresis (HPCE). Beckman P/ACE System 5010 apparatus equipped with a UV detector and a Beckman untreated fused-silica capillary (57 cm 75 mm, 50 cm effective length) was used. Before sample injection, the capillary was rinsed with 1 molL-1 sodium hydroxide solution and running buffer for 5 min, respectively. A voltage of 20 kV was applied for the separation. Pressure injection was 586 kPa for 6 seconds, and the wavelength of detector was 254 nm. The running time was 20 min at 20 oC. The effect of humidity and heat on the contents of nucleosides from natural Cordyceps sinensis and cultured Cordyceps mycelia was observed for 1, 3, 5 and 10 days at temperature 40 oC, and relative humidity 75%. Results: The content of nucleosides from natural Cordyceps sinensis was higher than that from cultured Cordyceps mycelia. But the contents of nucleosides from freshly collected natural Cordyceps sinensis were very low, even below the limit of quantitation. The contents of nucleosides from natural Cordyceps sinensis were significantly increased by humidity and heat, but this phenomenon was not observed in cultured Cordyceps mycelia. Conclusion: There are differences between the nucleosides from natural Cordyceps sinensis and cultured Cordyceps mycelia. The nucleosides in natural Cordyceps sinensis may be derived from the degradation of nucleic acids. This implies that adenosine being used for the quality control of natural Cordyceps sinensis may have to be reconsidered.
基金Supported by National Natural Science Foundation of China(20776058)New Century Training Programme Foundation for the Talents by the State Education Commission (NCET-06-0646)~~
文摘[Objective] The aim was to isolate and identify a taxol-producing endophytic fungus from Taxus media. [Method] 32 strains of endophytic fungi were identified form the inner bark of T. media,and their fermentation products were detected by high performance liquid chromatography (HPLC). [Result] Through the screening,a strain of taxol-producing endophytic fungi M57 was obtained,which could produce 45-50 μg/L of taxol,and M57 was defined as Rhizopus sp. through morphological observation and 18S rDNA sequence analysis. [Conclusion] The finding of Rhizopus sp. M57 provided a promising strain for producing taxol with taxol-producing fungi fermentation process.
基金Supported by Key Projects in the National Science&Technology Pillar Program~~
文摘The research explored adaptability maize seed coating agent in spring sowing and effects of ultra-fine powder shaped areas in northeast China. The results showed that germination potential and rate both improved around 5% and the num- ber of root increased by 4% in the treatments with ultra-fine powder shaped maize seed coating agent compared with the control group without seed coating. What's more, the treatments with ultra-fine powder shaped maize seed coating agent took advantages in terms of fresh weight of seeding, stem diameter, and dry weight. It is notable that control effects on underground insects performed the best, with per- centage over 94%, which is more excellent relative to other agents in markets. Therefore, ultra-fine powder shaped maize seed coating agent can be widely applied in spring sowing areas in northeast China, without side effects.
文摘The paper analyzes the definite place of industrial building in the history of urban development. Due to the development of urban economy and the transition of the traditional industrial structure, many historic cities and towns both at home and abroad have witnessed a large scale "demolition" and abandonment of industrial buildings in urban renewal during last 30 years. Consequently, it has been leading to the discontinuity of urban cultural and historic context. The paper discusses and expounds the clas...
文摘Fenlong green ecological agriculture technology (Fenlong technology), a new smash ridging farming method developed by Guangxi Academy of Agdcultural Sciences, has been elected as the recommended cultivation technique by the Ministry of Agriculture of China. It replaces the traditional plowshare with spiral drill, and its tilth depth is twice deeper than that by tractor tilthing. It also extends soil nutrient, moisture, oxygen and microorganism, the so-called "Four pools". Soil nutrient, oxygen, microorganism, light and rainfall use ratio is increased by 10%-100%, creating a platform for natural increase of more than 10% of crop yield. Its application to over 20 kinds of crops in 21 provinces has proved that the yield increases 10-30% with quality enhancing 5% and double water retaining capacity but no more input. When the application area of Fenlong could reach 67 million hm2, the amount of fertilizer can be reduced by 40-50 billion kg, saving 120-150 billion Yuan. In this paper, we put forward the strategy of "4+1" (arable, saline-alkali soil, grasslands, Sponge City + rivers) green development in China, and deepened the Fenlong cultivated tilled layer from 16.5 cm to 35 cm for 67 million hm2 arable land, ridged 13.3 million hm2 of saline-alkali soil for 35 cm, and also 35 cm for 67 million hm2 degraded steppe, which could have the following 3 effects: first, the 147 million hm2 of land with Fenlong cultivation could increase loosing soil to 315.491 billion m3, in* creasing by 159.26% for 120 million hm2 of arable land with the average tilled layer of 16.5 cm, which has loosing soil of only 198.1 billion m3, that is, the space of the land increases 1.6 times. Second, every hectare of plowland could store up to 450 m3/hm2 of natural rainfall, and the unused 60 m3 of saline-alkali soil and grasslands could store water of 102 billion m3, showing an increase of over 88.89% for the current plowland storage of 54 billion m3 at now, that is, double the natural rainfall storage capacity. Third, the two multiple increase of natural resources application can bring trillions of resource activation, environmental cleaning, food security, citizens, health, economic, ecological and social benefits, and makes the Chinese nation move forward in green development. Its application in "big scientific research" and "One Belt And One Road" will contribute Chinese strength to the world.
基金The National high Technology Research and Development Program of China (No.2006AA10A204)The National science & Technology Pillar Program (No. 2006BAD06A17)
文摘In order to develop an anti-FMDV Asial type monoclonal antibody (mAb), BABL/c mice were immunized with recombinant FMDV VP1 protein. Three mAbs, 1B8, 5El and 5E2, were then further optimized. The result indicated that prepared anti-FMDV Asial mAbs had no cross-reactivity with Swine vesicular disease (SVD) and FMDV O, A and C type antigen. Their titers in abdomen liquor were 1:5×10^6, 1:2×10^6 and 1:5×10^6, respectively. 1B8 was found to be of IgG1 subtype, 5El and 5E2 belonged to IgG2b subtype. In this study, the prepared mAbs are specific for detecting FMDV type Asia1, and is potentially useful for pen-side diagnosis.
基金National Natural Science Foundation of China (No. 51078068)Fundamental Research Funds for the Central Universities,China (No. 11D11314)Natural Science Foundation of Shanghai,China (No. 10ZR1401300)
文摘A model of an irreversible proton exchange membrane (PEM) fuel cell working at steady-state was established, in which overpotenfials, internal currents, and crossover losses were taken into account. The expressions of some key parameters of the fuel cell were derived from the point of electrochemistry and thermodynamics. Based on the irreversible model of the PEM fuel ceil, the influence of multi-irreversibilities on fuel cell performance were characterized and compared systematically. The general performance characteristic curves were generated. Moreover, when the electrical circuit was dosed with a load in it, the relations between the load resistance and power output density and efficiency were analyzed. The results provide a theoretical basis for both the operation and optimal design of real PEM fuel cells.
文摘A synthetic technology for the production of methyl-ethyl-ketone (MEK) is introduced, which makes use of butylene catalytic hydration to produce secondary butanol (SBA) and this is followed by dehydrogenation of SBA. By optimizing the operating conditions of a commercial unit, the running results showed that the recom-mended process was successful and that the catalysts had high activity, good selectivity, and long lifespan, which ensured long-term running in commercial units.
基金CNPq,FAPERJ and CAPES through the PDSE and Program and Human Resources Program 13 of the National Petroleum Agency (ANP-PRH 13)
文摘1,3-Propanediol,traditionally obtained from fossils,has numerous industrial applications,including use in the production of high performance polymers.The microbial production of 1,3-propanediol presents several opportunities,and the final purity grade determines its price and commercial viability.The development of novel separation technology could improve the economic viability of the bioproduction of 1,3-propanediol.Thus,we investigated salting-out extraction as a novel process for 1,3-propanediol recovery from fermentation broth.Initially,a screening for the best salt/solvent combination was conducted and then optimized using the response surface methodology.The solvents studied were methanol,ethanol,isopropanol and acetone,and the salts examined were K_2HPO_4,Na_2CO_3,K_2CO_3,(NH_4)_2SO_4,NaHPO_4,K_3PO_4 and C_6H_5NaO_7.The optimal extraction system consisted of 34 wt%K_3PO_4,28 wt% ethanol,and 38 wt% fermentation broth containing 23.0 g·L^(-1)1,3-propanediol,which gave the highest partition coefficient of 33 and recovery yield of 97%.The results demonstrated that salting-out extraction was a promising method for 1,3-propanediol recovery from fermentation broth.