[Objective] This study aimed to construct the full-length cDNA library for ger- minating seeds of Phyllostachys heterocycla [Method] Germinating seeds of P. hetero- cycla were used as experimental materials to constru...[Objective] This study aimed to construct the full-length cDNA library for ger- minating seeds of Phyllostachys heterocycla [Method] Germinating seeds of P. hetero- cycla were used as experimental materials to construct the full-length cDNA library by using Oligo-capping method. [Result] The constructed library has a total capacity of 6.5×10^6 recombinant clones, and a low proportion of clones without inserted frag- ments; the size of inserted fragments ranges between 0.3-5.0 kb, with strict classifi- cation and ideal consistency. Furthermore, the proportion of clones harboring long in- serted fragments (1.0-5.0 kb) is as high as 30%, achieving the standard for high- quality full-length cDNA library. [Conclusion] The full-length cDNA library of germinat- ing seeds of P. heterocycla was successfully constructed, which laid important foun- dation for the functional genomics research of bamboo plants.展开更多
This paper focuses on exporting relational data into extensible markup language (XML). First, the characteristics of both relational schemas represented by E-R diagrams and XML document type definitions (DTDs) are an...This paper focuses on exporting relational data into extensible markup language (XML). First, the characteristics of both relational schemas represented by E-R diagrams and XML document type definitions (DTDs) are analyzed. Secondly, the corresponding mapping rules are proposed. At last an algorithm based on edge tables is presented. There are two key points in the algorithm. One is that the edge table is used to store the information of the relational dictionary, and this brings about the efficiency of the algorithm. The other is that structural information can be obtained from the resulting DTDs and other applications can optimize their query processes using the structural information.展开更多
In 2008, a green tide broke out before the sailing competition of the 29th Olympic Games in Qingdao. The causative species was determined to be Enteromorpha prolifera (Ulva prolifera O. F. Miiller), a familiar green...In 2008, a green tide broke out before the sailing competition of the 29th Olympic Games in Qingdao. The causative species was determined to be Enteromorpha prolifera (Ulva prolifera O. F. Miiller), a familiar green macroalga along the coastline of China. Rapid accumulation of a large biomass of floating U. prolifera prompted research on different aspects of this species. In this study, we constructed a nonnormalized cDNA library from the thalli of U. prolifera and acquired 10072 high-quality expressed sequence tags (ESTs). These ESTs were assembled into 3 519 nonredundant gene groups, including 1 446 clusters and 2 073 singletons. After annotation with the nr database, a large number of genes were found to be related with chloroplast and ribosomal protein, GO functional classification showed 1 418 ESTs participated in photosynthesis and 1 359 ESTs were responsible for the generation of precursor metabolites and energy. In addition, rather comprehensive carbon fixation pathways were found in U. prolifera using KEGG. Some stress-related and signal transduction-related genes were also found in this study. All the evidences displayed that U. prolifera had substance and energy foundation for the intense photosynthesis and the rapid proliferation. Phylogenetic analysis of cytochrome c oxidase subunit I revealed that this green-tide causative species is most closely affiliated to Pseudendoclonium akinetum (Ulvophyceae).展开更多
Objective: The aim of our study was to compare the risk of main postoperative complications of patients un- derwent total thyroidectomy (TT), near total thyroidectomy (NTT) and subtotal thyroidectomy (ST) for t...Objective: The aim of our study was to compare the risk of main postoperative complications of patients un- derwent total thyroidectomy (TT), near total thyroidectomy (NTT) and subtotal thyroidectomy (ST) for treating BMNG. Methods: Electronic databases including Cochrane Controlled Trials Register, EMbase, Medline, SCI Expanded-ISI, NTIS, SIGLE and CNKI were searched. Eligible studies were randomized controlled trials which compared the incidence of postoperative complications of TT with ST/NTT for treating BMNG. Data extraction was using predefined data fields and assessment of methodological quality with the GRADE approach. A ManteI-Haenszel random-effects model was used and the effect sizes were expressed by using relative risks and 95% confidence intervals. Results: Four studies including 881 participants that met inclusion criteria were analyzed. Compared with ST/NTT, TT was associated with increased risk of transient RLNI (recur- rent laryngeal nerve injuries) (relative risk 2.18, 95% confidence interval 1.08 to 4.42; P 〈 0.05) and transient hypocalcemia (3.79, 1.64 to 8.77; P 〈 0.05), but there were no statistically significant differences in permanent RLNI (1.36, 0.31 to 6.02; P 〉 0.05) and permanent hypocalcemia (2.37, 0.35 to 15.97; P 〉 0.05). The quality of evidence for each individual outcome was ultimately rated as moderate (permanent RLNI and permanent hypocalcemia), low (transient RLNI and transient hypocalce- mia). Conclusion: For treating BMNG with thyroidectomy, there were no statistically significant differences in postoperative complications between TT and ST/NTT on a long view.展开更多
In this paper,based on the collected data and earthquake field investigation,characteristics of the MS4.1 Hujiaping earthquake of November 22,2008 at Guizhou town in Zigui county,Hubei Province and the geological and ...In this paper,based on the collected data and earthquake field investigation,characteristics of the MS4.1 Hujiaping earthquake of November 22,2008 at Guizhou town in Zigui county,Hubei Province and the geological and hydrogeological conditions and seismicity background of the area are analyzed,and the earthquake disaster is presented. Some scientific issues relating to earthquake precursors and the cause of the earthquake is discussed. The authors consider that the earthquake is a tectonic type reservoir-induced earthquake,occurring along the Xiannvshan fault under the joint action of reservoir water loading and water infiltration,and that there were certain suspected anomalies appearing in the gravity field before the earthquake. The cause of the earthquake may also be related to the effect of the Wenchuan earthquake on the local stress field.展开更多
基金Supported by Specialized Fund for the Basic Research Operating Expenses Program of International Centre for Bamboo and Rattan(163201300812618-7)Special Fund for Research and Development of Forestry Nonprofit Industry(200704001)~~
文摘[Objective] This study aimed to construct the full-length cDNA library for ger- minating seeds of Phyllostachys heterocycla [Method] Germinating seeds of P. hetero- cycla were used as experimental materials to construct the full-length cDNA library by using Oligo-capping method. [Result] The constructed library has a total capacity of 6.5×10^6 recombinant clones, and a low proportion of clones without inserted frag- ments; the size of inserted fragments ranges between 0.3-5.0 kb, with strict classifi- cation and ideal consistency. Furthermore, the proportion of clones harboring long in- serted fragments (1.0-5.0 kb) is as high as 30%, achieving the standard for high- quality full-length cDNA library. [Conclusion] The full-length cDNA library of germinat- ing seeds of P. heterocycla was successfully constructed, which laid important foun- dation for the functional genomics research of bamboo plants.
文摘This paper focuses on exporting relational data into extensible markup language (XML). First, the characteristics of both relational schemas represented by E-R diagrams and XML document type definitions (DTDs) are analyzed. Secondly, the corresponding mapping rules are proposed. At last an algorithm based on edge tables is presented. There are two key points in the algorithm. One is that the edge table is used to store the information of the relational dictionary, and this brings about the efficiency of the algorithm. The other is that structural information can be obtained from the resulting DTDs and other applications can optimize their query processes using the structural information.
基金Supported by the Scientific and Technical Supporting Programs of China (2008BAC49B01)the National Natural Science Foundation of China (No. 30830015)
文摘In 2008, a green tide broke out before the sailing competition of the 29th Olympic Games in Qingdao. The causative species was determined to be Enteromorpha prolifera (Ulva prolifera O. F. Miiller), a familiar green macroalga along the coastline of China. Rapid accumulation of a large biomass of floating U. prolifera prompted research on different aspects of this species. In this study, we constructed a nonnormalized cDNA library from the thalli of U. prolifera and acquired 10072 high-quality expressed sequence tags (ESTs). These ESTs were assembled into 3 519 nonredundant gene groups, including 1 446 clusters and 2 073 singletons. After annotation with the nr database, a large number of genes were found to be related with chloroplast and ribosomal protein, GO functional classification showed 1 418 ESTs participated in photosynthesis and 1 359 ESTs were responsible for the generation of precursor metabolites and energy. In addition, rather comprehensive carbon fixation pathways were found in U. prolifera using KEGG. Some stress-related and signal transduction-related genes were also found in this study. All the evidences displayed that U. prolifera had substance and energy foundation for the intense photosynthesis and the rapid proliferation. Phylogenetic analysis of cytochrome c oxidase subunit I revealed that this green-tide causative species is most closely affiliated to Pseudendoclonium akinetum (Ulvophyceae).
文摘Objective: The aim of our study was to compare the risk of main postoperative complications of patients un- derwent total thyroidectomy (TT), near total thyroidectomy (NTT) and subtotal thyroidectomy (ST) for treating BMNG. Methods: Electronic databases including Cochrane Controlled Trials Register, EMbase, Medline, SCI Expanded-ISI, NTIS, SIGLE and CNKI were searched. Eligible studies were randomized controlled trials which compared the incidence of postoperative complications of TT with ST/NTT for treating BMNG. Data extraction was using predefined data fields and assessment of methodological quality with the GRADE approach. A ManteI-Haenszel random-effects model was used and the effect sizes were expressed by using relative risks and 95% confidence intervals. Results: Four studies including 881 participants that met inclusion criteria were analyzed. Compared with ST/NTT, TT was associated with increased risk of transient RLNI (recur- rent laryngeal nerve injuries) (relative risk 2.18, 95% confidence interval 1.08 to 4.42; P 〈 0.05) and transient hypocalcemia (3.79, 1.64 to 8.77; P 〈 0.05), but there were no statistically significant differences in permanent RLNI (1.36, 0.31 to 6.02; P 〉 0.05) and permanent hypocalcemia (2.37, 0.35 to 15.97; P 〉 0.05). The quality of evidence for each individual outcome was ultimately rated as moderate (permanent RLNI and permanent hypocalcemia), low (transient RLNI and transient hypocalce- mia). Conclusion: For treating BMNG with thyroidectomy, there were no statistically significant differences in postoperative complications between TT and ST/NTT on a long view.
文摘In this paper,based on the collected data and earthquake field investigation,characteristics of the MS4.1 Hujiaping earthquake of November 22,2008 at Guizhou town in Zigui county,Hubei Province and the geological and hydrogeological conditions and seismicity background of the area are analyzed,and the earthquake disaster is presented. Some scientific issues relating to earthquake precursors and the cause of the earthquake is discussed. The authors consider that the earthquake is a tectonic type reservoir-induced earthquake,occurring along the Xiannvshan fault under the joint action of reservoir water loading and water infiltration,and that there were certain suspected anomalies appearing in the gravity field before the earthquake. The cause of the earthquake may also be related to the effect of the Wenchuan earthquake on the local stress field.